LEAP Works! Outcomes of a Family-Based Nutrition Education and Physical Activity Promotion Program

Overweight is an endemic public health concern for children, adolescents, and adults. Reducing the prevalence of childhood overweight is a national health objective and nine million U.S. children, currently classified as overweight, require effective nutrition education and physical activity promotion services. The intent of this study was to evaluate the effectiveness of a family-based nutrition education and physical activity promotion program, Lifelong Eating and Activity Patterns (LEAP), as an effective approach for the prevention and treatment of child overweight. The study design was an eight-week intervention and participant files were analyzed retrospectively to ascertain information for the study variables. The main outcome measures were changes in body mass index (BMI), percent body fat, fitness level, and eating and activity behaviors for child and parent participants. Qualitative data gathered from an exit survey for both children and parents were used to assess perceived benefits, value, and effectiveness of the program. Participants included 107 boys (n=48) and girls (n=59) ages 7-14, mean age 10.6 years, and their accompanying parents. The child participants were either “at risk for overweight” (3%) or “overweight” (97%). The results showed a significant decrease in BMI for both child and parent participants and percent body fat for child participants. There was a significant increase in reported fruit and vegetable consumption, regular physical activity, and in fitness parameters for both child and parent participants. The qualitative exit survey results indicated that the LEAP program resulted in significant improvements in knowledge, attitude, and behavior. In conclusion, this family-based nutrition education and physical activity promotion program was effective in decreasing BMI and body fat, increasing fitness level, and eliciting positive changes in eating and activity attitudes and behavior in both child and parent participants

LEAP Works! Outcomes of a Family-based Nutrition Education and Physical Activity Promotion Program

Abstract Overweight is an endemic public health concern for children, adolescents, and adults. Reducing the prevalence of childhood overweight is a national health objective and nine million U.S. children, currently classified as overweight, require effective nutrition education and physical activity promotion services. The intent of this study was to evaluate the effectiveness of a family-based nutrition education and physical activity promotion program, Lifelong Eating and Activity Patterns (LEAP), as an effective approach for the prevention and treatment of child overweight. The study design was an eight-week intervention and participant files were analyzed retrospectively to ascertain information for the study variables. The main outcome measures were changes in body mass index (BMI), percent body fat, fitness level, and eating and activity behaviors for child and parent participants. Qualitative data gathered from an exit survey for both children and parents were used to assess perceived benefits, value, and effectiveness of the program. Participants included 107 boys (n=48) and girls (n=59) ages 7-14, mean age 10.6 years, and their accompanying parents. The child participants were either "at risk for overweight" (3%) or "overweight" (97%). The results showed a significant decrease in BMI for both child and parent participants and percent body fat for child participants. There was a significant increase in reported fruit and vegetable consumption, regular physical activity, and in fitness parameters for both child and parent participants. The qualitative exit survey results indicated that the LEAP program resulted in significant improvements in knowledge, attitude, and behavior. In conclusion, this family-based nutrition education and physical activity promotion program was effective in decreasing BMI and body fat, increasing fitness level, and eliciting positive changes in eating and activity attitudes and behavior in both child and parent participants

Rapid genome editing by CRISPR-Cas9-POLD3 fusion

Precision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR) to introduce custom DNA sequences to target sites. The HDR editing efficiency varies between cell types and genomic sites, and the sources of this variation are incompletely understood. Here, we have studied the effect of 450 DNA repair protein-Cas9 fusions on CRISPR genome editing outcomes. We find the majority of fusions to improve precision genome editing only modestly in a locus- and cell-type specific manner. We identify Cas9-POLD3 fusion that enhances editing by speeding up the initiation of DNA repair. We conclude that while DNA repair protein fusions to Cas9 can improve HDR CRISPR editing, most need to be optimized to the cell type and genomic site, highlighting the diversity of factors contributing to locus-specific genome editing outcomes.Peer reviewe

The Helicobacter pylori Genome Project : insights into H. pylori population structure from analysis of a worldwide collection of complete genomes

Helicobacter pylori, a dominant member of the gastric microbiota, shares co-evolutionary history with humans. This has led to the development of genetically distinct H. pylori subpopulations associated with the geographic origin of the host and with differential gastric disease risk. Here, we provide insights into H. pylori population structure as a part of the Helicobacter pylori Genome Project (HpGP), a multi-disciplinary initiative aimed at elucidating H. pylori pathogenesis and identifying new therapeutic targets. We collected 1011 well-characterized clinical strains from 50 countries and generated high-quality genome sequences. We analysed core genome diversity and population structure of the HpGP dataset and 255 worldwide reference genomes to outline the ancestral contribution to Eurasian, African, and American populations. We found evidence of substantial contribution of population hpNorthAsia and subpopulation hspUral in Northern European H. pylori. The genomes of H. pylori isolated from northern and southern Indigenous Americans differed in that bacteria isolated in northern Indigenous communities were more similar to North Asian H. pylori while the southern had higher relatedness to hpEastAsia. Notably, we also found a highly clonal yet geographically dispersed North American subpopulation, which is negative for the cag pathogenicity island, and present in 7% of sequenced US genomes. We expect the HpGP dataset and the corresponding strains to become a major asset for H. pylori genomics

Rapid genome editing by CRISPR-Cas9-POLD3 fusion.

Precision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR) to introduce custom DNA sequences to target sites. The HDR editing efficiency varies between cell types and genomic sites, and the sources of this variation are incompletely understood. Here, we have studied the effect of 450 DNA repair protein-Cas9 fusions on CRISPR genome editing outcomes. We find the majority of fusions to improve precision genome editing only modestly in a locus- and cell-type specific manner. We identify Cas9-POLD3 fusion that enhances editing by speeding up the initiation of DNA repair. We conclude that while DNA repair protein fusions to Cas9 can improve HDR CRISPR editing, most need to be optimized to the cell type and genomic site, highlighting the diversity of factors contributing to locus-specific genome editing outcomes

Role of proton pump inhibitors dosage and duration in Helicobacter pylori eradication treatment: Results from the European Registry on H. pylori management

Background: Management of Helicobacter pylori (H. pylori) infection requires co-treatment with proton pump inhibitors (PPIs) and the use of antibiotics to achieve successful eradication.Aim: To evaluate the role of dosage of PPIs and the duration of therapy in the effectiveness of H. pylori eradication treatments based on the 'European Registry on Helicobacter pylori management' (Hp-EuReg).Methods: Hp-EuReg is a multicentre, prospective, non-interventionist, international registry on the routine clinical practice of H. pylori management by European gastroenterologists. All infected adult patients were systematically registered from 2013 to 2022.Results: Overall, 36,579 patients from five countries with more than 1000 patients were analysed. Optimal (>= 90%) first-line-modified intention-to-treat effectiveness was achieved with the following treatments: (1) 14-day therapies with clarithromycin-amoxicillin-bismuth and metronidazole-tetracycline-bismuth, both independently of the PPI dose prescribed; (2) All 10-day (except 10-day standard triple therapy) and 14-day therapies with high-dose PPIs; and (3) 10-day quadruple therapies with clarithromycin-amoxicillin-bismuth, metronidazole-tetracycline-bismuth, and clarithromycin-amoxicillin-metronidazole (sequential), all with standard-dose PPIs. In first-line treatment, optimal effectiveness was obtained with high-dose PPIs in all 14-day treatments, in 10- and 14-day bismuth quadruple therapies and in 10-day sequential with standard-dose PPIs. Optimal second-line effectiveness was achieved with (1) metronidazole-tetracycline-bismuth quadruple therapy for 14- and 10 days with standard and high-dose PPIs, respectively; and (2) levofloxacin-amoxicillin triple therapy for 14 days with high-dose PPIs. None of the 7-day therapies in both treatment lines achieved optimal effectiveness.Conclusions: We recommend, in first-line treatment, the use of high-dose PPIs in 14-day triple therapy and in 10-or 14-day quadruple concomitant therapy in first-line treatment, while standard-dose PPIs would be sufficient in 10-day bismuth quadruple therapies. On the other hand, in second-line treatment, high-dose PPIs would be more beneficial in 14-day triple therapy with levofloxacin and amoxicillin or in 10-day bismuth quadruple therapy either as a three-in-one single capsule or in the traditional scheme

Supervisory alliance: Key to positive alliances and outcomes in home-based parenting support?

Objectives: This study investigated whether the supervisory alliance between professionals and supervisors contributes to strong client-professional alliances and positive outcomes of home-based parenting support provided by youth care organizations. Methods: Multi-informant self-report supervisory alliance, alliance, and outcome data from 124 parents (M age = 39.83 years, SD = 6.98), professionals (n = 84, M age = 43.66 years, SD = 10.46), and supervisors (n = 26, M age = 47.18 years, SD = 8.28) collected early and late in care were analyzed using structural equation modeling. Results: A stronger professional-reported supervisory alliance was related to a stronger professional-reported alliance early in care (beta = 0.27, p < 0.01), and predicted higher levels of parent-reported satisfaction with care (beta = 0.19, p < .05; beta = 0.25, p < 0.01), and professional-reported satisfaction with care (beta = 0.21, p < 0.01). A stronger supervisory alliance reported by supervisors predicted parent-reported improvement in parent functioning (beta = 0.26, p < 0.05), and higher levels of professional-reported satisfaction with care (beta = 0.19, p < 0.05; beta = .14, p < 0.05). Finally, effects of professional-reported supervisory alliance on professional-reported satisfaction with care were mediated through higher levels of professional-reported alliance (beta = 0.06, p < 0.05; beta = .07, p < 0.05). Conclusions: A strong supervisory alliance may relate to strong alliances and positive outcomes of home-based parenting support. Future research needs to identify factors that contribute to strong supervisory alliances and explain linkages between the supervisory alliance, the alliance, and outcomes