124 research outputs found
Relaxation Scenarios in a Mixture of Large and Small Spheres: Dependence on the Size Disparity
We present a computational investigation on the slow dynamics of a mixture of
large and small soft spheres. By varying the size disparity at a moderate fixed
composition different relaxation scenarios are observed for the small
particles. For small disparity density-density correlators exhibit moderate
stretching. Only small quantitative differences are observed between dynamic
features for large and small particles. On the contrary, large disparity
induces a clear time scale separation between the large and the small
particles. Density-density correlators for the small particles become extremely
stretched, and display logarithmic relaxation by properly tuning the
temperature or the wavevector. Self-correlators decay much faster than
density-density correlators. For very large size disparity, a complete
separation between self- and collective dynamics is observed for the small
particles. Self-correlators decay to zero at temperatures where density-density
correlations are frozen. The dynamic picture obtained by varying the size
disparity resembles features associated to Mode Coupling transition lines of
the types B and A at, respectively, small and very large size disparity. Both
lines might merge, at some intermediate disparity, at a higher-order point, to
which logarithmic relaxation would be associated. This picture resembles
predictions of a recent Mode Coupling Theory for fluids confined in matrixes
with interconnected voids [V. Krakoviack, Phys. Rev. Lett. {\bf 94}, 065703
(2005)].Comment: Journal of Chemical Physics 125, 164507 (2006
Anomalous Dynamic Arrest in a Mixture of Big and Small Particles
We present molecular dynamics simulations on the slow dynamics of a mixture
of big and small soft-spheres with a large size disparity. Dynamics are
investigated in a broad range of temperature and mixture composition. As a
consequence of large size disparity, big and small particles exhibit very
different relaxation times. As previously reported for simple models of
short-ranged attractive colloids and polymer blends, several anomalous dynamic
features are observed: i) sublinear behavior for mean squared displacements,
ii) concave-to-convex crossover for density-density correlators, by varying
temperature or wavevector, iii) logarithmic decay for specific wavevectors of
density-density correlators. These anomalous features are observed over time
intervals extending up to four decades, and strongly resemble predictions of
the Mode Coupling Theory (MCT) for state points close to higher-order MCT
transitions, which originate from the competition between different mechanisms
for dynamic arrest. For the big particles we suggest competition between
soft-sphere repulsion and depletion effects induced by neighboring small
particles. For the small particles we suggest competition between bulk-like
dynamics and confinement, respectively induced by neighboring small particles
and by the slow matrix of big particles. By increasing the size disparity, a
new relaxation scenario arises for the small particles. Self-correlators decay
to zero at temperatures where density-density correlations are frozen. The
behavior of the latters resembles features characteristic of type-A MCT
transitions, defined by a zero value of the critical non-ergodicity parameter.Comment: Version 2. Added major new result
Rapid Differential Diagnosis between Extrapulmonary Tuberculosis and Focal Complications of Brucellosis Using a Multiplex Real-Time PCR Assay
BACKGROUND: Arduous to differ clinically, extrapulmonary tuberculosis and focal complications of brucellosis remain important causes of morbidity and mortality in many countries. We developed and applied a multiplex real-time PCR assay (M RT-PCR) for the simultaneous detection of Mycobacterium tuberculosis complex and Brucella spp. METHODOLOGY: Conventional microbiological techniques and M RT-PCR for M. tuberculosis complex and Brucella spp were performed on 45 clinical specimens from patients with focal complications of brucellosis or extrapulmonary tuberculosis and 26 control samples. Fragments of 207 bp and 164 bp from the conserved region of the genes coding for an immunogenic membrane protein of 31 kDa of B. abortus (BCSP31) and the intergenic region SenX3-RegX3 were used for the identification of Brucella and M. tuberculosis complex, respectively. CONCLUSIONS: The detection limit of the M RT-PCR was 2 genomes per reaction for both pathogens and the intra- and inter-assay coefficients of variation were 0.44% and 0.93% for Brucella and 0.58% and 1.12% for Mycobacterium. M RT-PCR correctly identified 42 of the 45 samples from patients with tuberculosis or brucellosis and was negative in all the controls. Thus, the overall sensitivity, specificity, PPV and NPV values of the M RT PCR assay were 93.3%, 100%, 100% and 89.7%, respectively, with an accuracy of 95.8% (95% CI, 91.1%-100%). Since M RT-PCR is highly reproducible and more rapid and sensitive than conventional microbiological tests, this technique could be a promising and practical approach for the differential diagnosis between extrapulmonary tuberculosis and focal complications of brucellosis
Effect of biochar amendment on morphology, productivity and water relations of sunflower plants under non-irrigation conditions
páginas.-- 6 figuras.-- 2 tablas.-- 56 referencias.-- Supplementary data to this article can be found online at http://dx.doi.org/10.1016/j.catena.2016.07.037.Three biochars (B1: pine wood, B2: paper-sludge, B3: sewage-sludge) produced under controlled pyrolysis conditions and one produced in kilns (B4: grapevine wood) were used as organic ameliorants in a Calcic Cambisol, which represents a typical agricultural soil of the Mediterranean region. This field study was performed with plants of sunflower (Helianthus annuus L.) at the experimental station “La Hampa”, located in the Guadalquivir river valley (SW Spain). The soil was amended with doses equivalent to 1.5 and 15 t ha− 1 of the four biochars in two independent plantations. In addition, un-amended plots were prepared in both experiments for comparison purposes. The major goal of this study was the assessment of the effect of biochar amendment on the physiology and development of sunflower plants at field conditions. During most of the growing period plants of un-amended and amended plots showed no stress symptoms either by their appearance or by stress-sensitive biochemical parameters such as the stability of the photosystem II (QY). Biochar addition had no effect on seed germination. Addition of 1.5 t ha− 1 biochar did not significantly change the pH of the soil, its electrical conductivity (EC) or its water holding capacity (WHC). Concomitantly the plant development and plant biomass production remained unaltered. Amendments with 15 t ha− 1 slightly increased the WHC of the soil but showed no lasting impact on the soil pH. It stimulated plant growth and led to a greater leaf area, larger plant stems and wider inflorescences of the sunflower plants than those cultivated on the un-amended soil. At the end of the experiment, biochar amendment of soil caused no significant increase of the total biomass production excepting B4, the biochar with the lowest capacity of water retention, which exhibited the highest vegetative growth and seed production. The lack of rain during the last weeks caused a water shortage in the culture that produced greater QY loss in non-amended plants. Interestingly, better growth of amended plants during the drought period correlated with higher reduction of stomatal conductance, indicating that the greater water use efficiency is at the origin of the better crop performance of biochar-amended plants. This finding points to the agronomic relevance of biochar amendment of Mediterranean rain fed crops.The Marie Skłodowska-Curie actions of the European Union's FP7 People Programme (REA grant agreement no PCIG12-GA-2012-333784-Biocharisma project) and the Spanish Ministry of Economy and Competitiveness (MINECO) (project PCGL2012-37041) are thanked for the financial support of the present study. The Spanish Ministry of Education, Culture and Sport is acknowledged for funding the FPU fellowship (FPU 13/05831) of Marina Paneque, and the contract of Juan De Dios Franco-Navarro (AGL2009-08339/AGR). The European Biochar Network (Biochar as option for sustainable resource management-COST action TD1107) and Bodegas Torres (Spain) are acknowledged for providing the biochar samples. M. Velasco is gratefully acknowledged for her technical assistance at field.Peer reviewe
Chloride regulates leaf cell size and water relations in tobacco plants
19 páginas.-- 9 figuras.-- 5 tablas.-- 77 referencias.-- Supplementary Data: Supplementary_figures_S1_S7___Tables_S1_S7.pdfChloride (Cl–) is a micronutrient that accumulates to macronutrient levels since it is normally available in nature and actively taken up by higher plants. Besides a role as an unspecific cell osmoticum, no clear biological roles have been explicitly associated with Cl– when accumulated to macronutrient concentrations. To address this question, the glycophyte tobacco (Nicotiana tabacum L. var. Habana) has been treated with a basal nutrient solution supplemented with one of three salt combinations containing the same cationic balance: Cl–-based (CL), nitrate-based (N), and sulphate+phosphate-based (SP) treatments. Under non-saline conditions (up to 5mM Cl–) and no water limitation, Cl– specifically stimulated higher leaf cell size and led to a moderate increase of plant fresh and dry biomass mainly due to higher shoot expansion. When applied in the 1–5mM range, Cl– played specific roles in regulating leaf osmotic potential and turgor, allowing plants to improve leaf water balance parameters. In addition, Cl– also altered water relations at the whole-plant level through reduction of plant transpiration. This was a consequence of a lower stomatal conductance, which resulted in lower water loss and greater photosynthetic and integrated water-use efficiency. In contrast to Cl–, these effects were not observed for essential anionic macronutrients such as nitrate, sulphate, and phosphate. We propose that the abundant uptake and accumulation of Cl– responds to adaptive functions improving water homeostasis in higher plants.This work was supported by the Spanish Ministry of Science and Innovation-FEDER grant AGL2009-08339/AGR. The help, expertise, and technical assistance of C. Rivero, A. Vázquez, S. Luque, B.J. Sañudo, F.J. Durán, Y. Pinto, and J. Espartero are gratefully acknowledged. We would like to extend our gratitude to the valuable reviews and contributions by the anonymous referees and the editor, Timothy Colmer, which helped us to improve the manuscript substantially.Peer reviewe
High and low molecular weight crossovers in the longest relaxation time dependence of linear cis-1,4 polyisoprene by dielectric relaxations
The dielectric relaxation of cis-1,4 Polyisoprene [PI] is sensitive not only to the local and segmental dynamics but also to the larger scale chain (end-to-end) fluctuations. We have performed a careful dielectric investigation on linear PI with various molecular weights in the range of 1 to 320 kg/mol. The broadband dielectric spectra of all samples were measured isothermally at the same temperature to avoid utilizing shift factors. For the low and medium molecular weight range, the comparisons were performed at 250 K to access both the segmental relaxation and normal mode peaks inside the available frequency window (1 mHz–10 MHz). In this way, we were able to observe simultaneously the effect of molecular mass on the segmental dynamics—related with the glass transition process—and on the end-to-end relaxation time of PI and thus decouple the direct effect of molecular weight on the normal mode from that due to the effect on the monomeric friction coefficient. The latter effect is significant for low molecular weight (M w < 33 kg/mol), i.e., in the range where the crossover from Rouse dynamics to entanglement limited flow occurs. Despite the conductivity contribution at low frequency, careful experiments allowed us to access to the normal mode signal for molecular weights as high as M w = 320 kg/mol, i.e., into the range of high molecular weights where the pure reptation behavior could be valid, at least for the description of the slowest chain modes. The comparison between the dielectric relaxations of PI samples with medium and high molecular weight was performed at 320 K. We found two crossovers in the molecular weight dependence of the longest relaxation time, the first around a molecular weight of 6.5 ± 0.5 kg/mol corresponding to the end of the Rouse regime and the second around 75 ± 10 kg/mol. Above this latter value, we find a power law compatible with exponent 3 as predicted by the De Gennes theory
The Role of Intramolecular Barriers on the Glass Transition of Polymers: Computer Simulations vs. Mode Coupling Theory
We present computer simulations of a simple bead-spring model for polymer
melts with intramolecular barriers. By systematically tuning the strength of
the barriers, we investigate their role on the glass transition. Dynamic
observables are analyzed within the framework of the Mode Coupling Theory
(MCT). Critical nonergodicity parameters, critical temperatures and dynamic
exponents are obtained from consistent fits of simulation data to MCT
asymptotic laws. The so-obtained MCT -exponent increases from standard
values for fully-flexible chains to values close to the upper limit for stiff
chains. In analogy with systems exhibiting higher-order MCT transitions, we
suggest that the observed large -values arise form the interplay
between two distinct mechanisms for dynamic arrest: general packing effects and
polymer-specific intramolecular barriers. We compare simulation results with
numerical solutions of the MCT equations for polymer systems, within the
polymer reference interaction site model (PRISM) for static correlations. We
verify that the approximations introduced by the PRISM are fulfilled by
simulations, with the same quality for all the range of investigated barrier
strength. The numerical solutions reproduce the qualitative trends of
simulations for the dependence of the nonergodicity parameters and critical
temperatures on the barrier strength. In particular, the increase of the
barrier strength at fixed density increases the localization length and the
critical temperature. However the qualitative agreement between theory and
simulation breaks in the limit of stiff chains. We discuss the possible origin
of this feature
Chloride Nutrition Regulates development, Water Balance and Drought Resistance in Plants
6 páginas.-- 5 figuras.-- 9 referencias.-- Poster presentado en el XII Luso-Spanish Symposium on Plant Water Relations – Water to Feed the World. 30th of September – 3rd of October (Evora) PortugalCl- is a strange micronutrient since actual Cl- concentration in plants is about two orders of magnitude higher than the content required as essential micronutrient. This accumulation requires a high cost of energy, and since Cl- is a major osmotically active solute in the vacuole, we propose that Cl- plays a role in the regulation of water balance in plants. We show here that, when accumulated to macronutrient levels, Cl- specifically regulates leaf cell elongation and water balance parameters, improving water relations at both the leaf tissue and the whole plant levels, increasing drought resistance in higher plants.This work was supported by the Spanish Ministry of Science and Innovation-FEDER grant AGL2009-08339/AGR.Peer Reviewe
Identification of Brucella by MALDI-TOF Mass Spectrometry. Fast and Reliable Identification from Agar Plates and Blood Cultures
BACKGROUND: MALDI-TOF mass spectrometry (MS) is a reliable method for bacteria identification. Some databases used for this purpose lack reference profiles for Brucella species, which is still an important pathogen in wide areas around the world. We report the creation of profiles for MALDI-TOF Biotyper 2.0 database (Bruker Daltonics, Germany) and their usefulness for identifying brucellae from culture plates and blood cultures. METHODOLOGY/PRINCIPAL FINDINGS: We created MALDI Biotyper 2.0 profiles for type strains belonging to B. melitensis biotypes 1, 2 and 3; B. abortus biotypes 1, 2, 5 and 9; B. suis, B. canis, B ceti and B. pinnipedialis. Then, 131 clinical isolates grown on plate cultures were used in triplicate to check identification. Identification at genus level was always correct, although in most cases the three replicates reported different identification at species level. Simulated blood cultures were performed with type strains belonging to the main human pathogenic species (B. melitensis, B. abortus, B. suis and B. canis), and studied by MALDI-TOF MS in triplicate. Identification at genus level was always correct. CONCLUSIONS/SIGNIFICANCE: MALDI-TOF MS is reliable for Brucella identification to the genus level from culture plates and directly from blood culture bottles
A school-based physical activity promotion intervention in children: rationale and study protocol for the PREVIENE Project
The lack of physical activity and increasing time spent in sedentary behaviours during childhood
place importance on developing low cost, easy-toimplement school-based interventions to increase physical
activity among children. The PREVIENE Project will evaluate the effectiveness of five innovative, simple, and feasible
interventions (active commuting to/from school, active Physical Education lessons, active school recess, sleep health
promotion, and an integrated program incorporating all 4 interventions) to improve physical activity, fitness,
anthropometry, sleep health, academic achievement, and health-related quality of life in primary school children. The PREVIENE Project will provide the information about the effectiveness and implementation of
different school-based interventions for physical activity promotion in primary school children.The PREVIENE Project was funded by the Spanish Ministry of Economy and
Competitiveness (DEP2015-63988-R, MINECO-FEDER).
MAG is supported by grants from the Spanish Ministry of Economy and
Competitivenes
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