Corruption and Development: The Need for International Investigations with a Multijurisdictional Approach Involving Multilateral Development Banks and National Authorities

We argue that while Multilateral Development Banks (“MDBs”) and national governments have mechanisms to fight corruption, the objectives and outcomes of these enforcement mechanisms diverge. MDBs are interested in the causes and effects of corruption from a development perspective and, as such, tend to sanction small and medium enterprises and individuals, while national governments are focused on a more punitive outcome, targeting larger multinational corporations. This article examines the enforcement objectives articulated in national legislation, namely the US Foreign and Corrupt Practices Act and its Canadian counterpart, the Corruption of Foreign Public Officials Act, as well as several Canadian cases, on the one hand, and the tools and outcomes of MDBs’ sanctions systems on the other. We conclude that national enforcement efforts and MDBs’ sanctions outcomes intersect in their fight against international corruption in that their results are complementary; the former punishing large-scale offenders while the latter ensuring the integrity of development projects

Corruption and Development: The Need for International Investigations with a Multijurisdictional Approach Involving Multilateral Development Banks and National Authorities

We argue that while Multilateral Development Banks (“MDBs”) and national governments have mechanisms to fight corruption, the objectives and outcomes of these enforcement mechanisms diverge. MDBs are interested in the causes and effects of corruption from a development perspective and, as such, tend to sanction small and medium enterprises and individuals, while national governments are focused on a more punitive outcome, targeting larger multinational corporations. This article examines the enforcement objectives articulated in national legislation, namely the US Foreign and Corrupt Practices Act and its Canadian counterpart, the Corruption of Foreign Public Officials Act, as well as several Canadian cases, on the one hand, and the tools and outcomes of MDBs’ sanctions systems on the other. We conclude that national enforcement efforts and MDBs’ sanctions outcomes intersect in their fight against international corruption in that their results are complementary; the former punishing large-scale offenders while the latter ensuring the integrity of development projects

Nanoscale Enzymatic Compartments in Tandem Support Cascade Reactions in Vitro

Compartmentalization at the nanoscale is fundamental in nature, where the spatial segregation of biochemical reactions within cells ensures optimal conditions for regulating metabolic pathways. Here, we present a nature-inspired approach to engineer enzymatic cascade reactions taking place between separate vesicular nanocompartments (polymersomes), each containing one enzyme type. We propose, by the selected combination of enzymes, an efficient solution to detoxify the harmful effect of uric acid and prevent the accumulation of the derived H2O2, both being associated with various pathological conditions (e.g., gout and oxidative stress). Fungal uricase and horseradish peroxidase combined to act in tandem, and they were separately encapsulated within nanocompartments that were equipped with channel porins as gates to allow passage of substrates and products from each step of the reaction. We established the molecular factors affecting the efficiency of the overall reaction, and the protective role of the compartments. Interestingly, the cascade reaction between separate nanocompartments was as efficient as for free enzymes in complex media, such as human serum. The nanocompartments were nontoxic toward cells, and more importantly, addition of the tandem catalytic nanocompartments to cells exposed to uric acid provided simultaneous detoxification of uric acid and the H2O2. Such catalytic nanocompartments can be used as a platform for understanding fundamental factors affecting intracellular communication and can introduce non-native metabolic reactions into living systems for therapeutic applications

Color heterogeneity of building surfaces: lean image processing approach for visible reflectance characterization performance

Daylighting availability and uniformity depend on the interior surface reflectance (). Currently, is obtained through suggested reference values (Illuminating Engineering Society 2012; CIBSE 2015; CIBSE/SLL 2011; CIBSE/SLL 2005), laboratory tests (ASTM E 903 (2012) describes a standardized procedure requiring a calibrated instrument and an as-built surface sample), or by on-site measures (based on luminance and illuminance differences). Novel methodologies compute it by integrating image processing and/or photometry, applied on false colour or HDR images. A simple procedure is needed for accurately assess, even in preliminary design phases, the reflectance of heterogeneous surface areas for new and historical buildings. In fact, heterogeneous surfaces (colour, texture, composition, ageing) difficult the accurate estimation of a representative reflectance value (̅) for building simulation, leading to daylighting performance deviation. This work presents a methodology, based on a per-pixel colour reflectivity () evaluation, to easily acquire an approximate value of the surface visible reflectance (̅). This approach gives a more global ̅ of all surface components, aiming to improve the accuracy of the modelled daylighting analysis. Additionally, the procedure is experimented over a sample reference test room

Differential expression of exosomal microRNAs in prefrontal cortices of schizophrenia and bipolar disorder patients

Exosomes are cellular secretory vesicles containing microRNAs (miRNAs). Once secreted, exosomes are able to attach to recipient cells and release miRNAs potentially modulating the function of the recipient cell. We hypothesized that exosomal miRNA expression in brains of patients diagnosed with schizophrenia (SZ) and bipolar disorder (BD) might differ from controls, reflecting either disease-specific or common aberrations in SZ and BD patients. The sources of the analyzed samples included McLean 66 Cohort Collection (Harvard Brain Tissue Resource Center), BrainNet Europe II (BNE, a consortium of 18 brain banks across Europe) and Boston Medical Center (BMC). Exosomal miRNAs from frozen postmortem prefrontal cortices with well-preserved RNA were isolated and submitted to profiling by Luminex FLEXMAP 3D microfluidic device. Multiple statistical analyses of microarray data suggested that certain exosomal miRNAs were differentially expressed in SZ and BD subjects in comparison to controls. RT-PCR validation confirmed that two miRNAs, miR-497 in SZ samples and miR-29c in BD samples, have significantly increased expression when compared to control samples. These results warrant future studies to evaluate the potential of exosome-derived miRNAs to serve as biomarkers of SZ and BD

Intoxicação experimental por Ipomoea carnea subsp. fistulosa em caprinos da Argentina: correlação clínico-patológica, com especial atenção ao sistema nervoso central

Ipomoea carnea subsp. fistulosa, aguapei or mandiyura, is responsible for lysosomal storage in goats. The shrub contains several alkaloids, mainly swansonine which inhibits lysosomal α-mannosidase and Golgi mannosidase II. Poisoning occurs by inhibition of these hydrolases. There is neuronal vacuolation, endocrine dysfunction, cardiovascular and gastrointestinal injury, and immune disorders. Clinical signs and pathology of the experimental poisoning of goats by Ipomoea carnea in Argentina are here described. Five goats received fresh leaves and stems of Ipomoea. At the beginning, the goats did not consume the plant, but later, it was preferred over any other forage. High dose induced rapid intoxication, whereas with low doses, the course of the toxicosis was more protracted. The goats were euthanized when they were recumbent. Cerebrum, cerebellum, medulla oblongata, pons and colliculi, were routinely processed for histology. In nine days, the following clinical signs developed: abnormal fascies, dilated nostrils and abnormal postures of the head, cephalic tremors and nystagmus, difficulty in standing. Subsequently, the goats had a tendency to fall, always to the left, with spastic convulsions. There was lack in coordination of voluntary movements due to Purkinje and deep nuclei neurons damage. The cochlear reflex originated hyperreflexia, abnormal posture, head movements and tremors. The withdrawal reflex produced flexor muscles hypersensitivity at the four legs, later depression and stupor. Abnormal responses to sounds were related to collicular lesions. Thalamic damage altered the withdrawal reflex, showing incomplete reaction. The observed cervical hair bristling was attributed to a thalamic regulated nociceptive response. Depression may be associated with agonists of lysergic acid contained in Ipomoea. These clinical signs were correlated with lesions in different parts of the CNS.Ipomoea carnea subsp. fistulosa, aguapeí ou mandiyura, causa uma doença de depósito lisossomal em caprinos. A planta contém vários alcalóides, principalmente swansonine, que inibe uma α-mannosidase lisossomal e uma mannosidase II do Golgi. A intoxicação ocorre pela inibição dessas hidrolases. Há vacuolização neuronal, disfunção endócrina, lesões cardiovasculares e gastrointestinais e distúrbios imunológicos. No presente trabalho é descrita a intoxicação experimental por Ipomoea carnea subsp. fistulosa em caprinos da Argentina. Cinco cabras receberam folhas frescas e ramos de Ipomoea. No começo eles não consumiram a planta, mas depois ela foi preferida a qualquer outras forrageiras. Altas doses induziram uma Pesq. Vet. Bras. 32(1):37-42, janeiro 2012 38 Elvio E. Ríos et al. intoxicação rápida, enquanto que com doses baixas demora mais tempo. As cabras foram sacrificadas quando ficavam em decúbito. Cérebro, cerebelo, medula oblonga, ponte e colículos foram processados rotineiramente para histologia. Aos nove dias, encontramos: fascie anormal, narinas dilatadas, posturas anormais da cabeça, tremores cefálicos e nistagmo, dificuldade para permanecer na estação. Posteriormente, presentearam a tendência de queda, sempre à esquerda, com convulsões espásticas. Os neurônios de Purkinje e os os núcleos profundos foram danificados. Como conseqüência, emergiu a falta de coordenação de movimentos voluntários. A hiper-reflexo coclear originou postura anormal, movimentos de cabeça e tremores. A retirada produz reflexos hipersensibilidade nos flexores dos quatro membros, depois depressão e letargia. As respostas anormais estão ligadas a lesões do colículo. Injurias no tálamo alteram o reflexo de retirada, mostrando uma reação incompleta. Os pelos eriçados da região cervical podem representar uma resposta nociceptiva também regulada pelo tálamo. A depressão pode estar associada com agonistas do ácido lisérgico presentes na Ipomoea. Esses sintomas estão relacionados com lesões em diferentes partes do SNC.Fil: Ríos, Elvio E.. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiologia del Nordeste; ArgentinaFil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiologia del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gimeno, Eduardo Juan. Universidad Nacional de la Plata; ArgentinaFil: Guidi, María G.. Universidad Nacional de la Plata; ArgentinaFil: Acosta de Pérez, Ofelia C.. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiologia del Nordeste; Argentin

Ouabain Enhances Cell-Cell Adhesion Mediated by beta1 Subunits of the Na(+),K(+)-ATPase in CHO Fibroblasts

Adhesion is a crucial characteristic of epithelial cells to form barriers to pathogens and toxic substances from the environment. Epithelial cells attach to each other using intercellular junctions on the lateral membrane, including tight and adherent junctions, as well as the Na(+),K(+)-ATPase. Our group has shown that non-adherent chinese hamster ovary (CHO) cells transfected with the canine beta1 subunit become adhesive, and those homotypic interactions amongst beta1 subunits of the Na(+),K(+)-ATPase occur between neighboring epithelial cells. Ouabain, a cardiotonic steroid, binds to the alpha subunit of the Na(+),K(+)-ATPase, inhibits the pump activity and induces the detachment of epithelial cells when used at concentrations above 300 nM. At nanomolar non-inhibiting concentrations, ouabain affects the adhesive properties of epithelial cells by inducing the expression of cell adhesion molecules through the activation of signaling pathways associated with the alpha subunit. In this study, we investigated whether the adhesion between beta1 subunits was also affected by ouabain. We used CHO fibroblasts stably expressing the beta1 subunit of the Na(+),K(+)-ATPase (CHO beta1), and studied the effect of ouabain on cell adhesion. Aggregation assays showed that ouabain increased the adhesion between CHO beta1 cells. Immunofluorescence and biotinylation assays showed that ouabain (50 nM) increases the expression of the beta1 subunit of the Na(+),K(+)-ATPase at the cell membrane. We also examined the effect of ouabain on the activation of signaling pathways in CHO beta1 cells, and their subsequent effect on cell adhesion. We found that cSrc is activated by ouabain and, therefore, that it likely regulates the adhesive properties of CHO beta1 cells. Collectively, our findings suggest that the beta1 subunit adhesion is modulated by the expression levels of the Na(+),K(+)-ATPase at the plasma membrane, which is regulated by ouabain

Current knowledge in pathophysiology and management of Budd-Chiari syndrome and non-cirrhotic non-tumoral splanchnic vein thrombosis

Budd-Chiari Syndrome (BCS) and non-cirrhotic non-tumoral portal vein thrombosis (NCPVT) are two rare disorders, with several similarities that are categorized under the term splanchnic vein thrombosis. Both disorders are frequently associated with an underlying pro-thrombotic disorder. They can cause severe portal hypertension and usually affect oung patients, negatively influencing life expectancy when the diagnosis and treatment is not done at an early stage. Yet, they have specific features that require individual considerations. The current review will focus on the available knowledge on pathophysiology, diagnosis and management of both entities. BCS is defined as the obstruction of hepatic venous outflow regardless of its causative mechanism or level of obstruction. This obstruction can be traced to the small hepatic venules up to the entrance of the inferior vein cava (IVC) into the right atrium. Hepatic outflow obstruction related to cardiac disease, pericardial disease or sinusoidal obstruction syndrome have different pathophysiological and clinical implications and are excluded from this definition. BCS is classified as primary when the obstruction originates in the vein and thrombosis is the main cause, or secondary when the vein is externally compressed (abscess, tumor). The focus of this review is on primary BCS. NCPVT refers to the presence of a thrombus in the main portal vein trunk and/or the left or right intrahepatic portal vein branches that may extend to the splenic vein and/or the superior or inferior mesenteric veins. Isolated splenic or mesenteric vein thrombosis are out of the scope of this review.Copyright © 2019. Published by Elsevier B.V

Revisión de tecnologías de membranas en la eliminación de metales pesados en aguas superficiales para consumo humano

[EN] Several sources contaminate heavy metals in surface waters. Among the main ones are industries and mining, in addition to natural geological pollution. Several international organizations consider heavy metals as toxic and dangerous. Globally, many bodies of surface water have concentrations of metal ions that exceed the allowed limits. This problem is more significant when water bodies contaminated with metals are used as sources of supply for cities. Therefore, there is an imminent need to apply efficient treatment technologies and with a high metal removal rate. This work makes a bibliographic review of the results obtained in the various experiences of the last 20 years, to identify which of the membrane filtration processes is the most promising for its application, considering different metal ions.[ES] Existen diversas fuentes que contaminan con metales pesados en las aguas superficiales. Entre las principales se encuentran las industrias y la minería, además de la contaminación geológica natural. Los metales pesados son considerados por varias organizaciones internacionales como tóxicos y/o peligrosos. A nivel mundial, muchos cuerpos de agua superficial presentan concentraciones de iones metálicos que exceden los límites permitidos. Esta problemática es mayor cuando los cuerpos de agua contaminados con metales se utilizan como fuentes de abastecimiento para ciudades. Observando lo anterior, existe la necesidad inminente de aplicar tecnologías de tratamiento eficientes y con alta tasa de remoción de metales. Este trabajo hace una revisión bibliográfica de los resultados obtenidos en las diversas experiencias de los últimos 20 años, con el fin de identificar cuál de los procesos de filtración por membranas es el más prometedor para su aplicación, considerando diferentes iones metálicos.Alvizuri-Tintaya, PA.; Villena Martínez, EM.; Torregrosa López, JI.; Lo-Iacono-Ferreira, VG.; Lora-García, J. (2020). Review of membrane technologies in the removal of heavy metals in surface waters for human consumption. Asociación Española de Dirección e Ingeniería de Proyectos (AIEPRO). 1130-1141. http://hdl.handle.net/10251/181059S1130114

Energy distribution of maxima and minima in a one-dimensional random system

We study the energy distribution of maxima and minima of a simple one-dimensional disordered Hamiltonian. We find that in systems with short range correlated disorder there is energy separation between maxima and minima, such that at fixed energy only one kind of stationary points is dominant in number over the other. On the other hand, in the case of systems with long range correlated disorder maxima and minima are completely mixed.Comment: 4 pages RevTeX, 1 eps figure. To appear in Phys. Rev.