Volatile oil composition and antioxidant activity of leaf of Chaerophyllum villosum Wall. ex DC from Uttrakhand, India

The genus Chaerophyllum, belonging to Apiaceae family, comprised of about 110 species which includes annual and perennial herbal plants widely distributed in temperate and sub temperate zones of Asia, Africa and Europe. Chaerophyllum villosum Wall. ex DC. was widely distributed in E. Asia Himalayas from India to Bhutan, Nepal and China and widely grows in moist shady places, road sides or open grassy places at elevations of 2100-3500 m. In high altitude tribes of Uttarakhand Himalaya (India) it was commonly known and sold in the name of ‘Ganjari’ widely used by people in food, spice and also as medicine. The volatile oil composition of leaf of Chaerophyllum villosum Wall. ex DC. (family: Apiaceae) were analyzed and compared using capillary GC and GC-MS. The leaf essential oil of C. villosum was dominated by monoterpene hydrocarbons (91.34%) represented by γ-terpinene (74.93%) as single major constituent followed by p-cymene (10.00%), terpinolene (2.93%) and β-pinene (2.54%), the antioxidant activity of leaf essential oil also evaluated. &nbsp

Vacuum thermal deposition of crystalline, uniform and stoichiometric CdS thin films in ambient H2S atmosphere

Crystalline, uniform and stoichiometric thin films of CdS have been fabricated on soda lime glass (SLG) substrates using vacuum thermal deposition method in the presence of hydrogen sulphide (H2S) atmosphere. The consequence of ambient H2S on the growth, quality and structure-property relationship of vacuum deposited CdS thin films has been investigated. The deposited films have been characterized by XRD, SEM with EDX analysis, AFM, XPS and optical spectroscopy. The physical characterization of as-deposited CdS films reveals that the films deposited in controlled H2S ambient are more crystalline, highly uniform and stoichiometric in comparison to films deposited without H2S atmosphere

Comparative Clinical study of Jatyadi Taila and Jatyadi Ghrita in the management of Dushta Vrana

Dushta Vrana is a common and frequently encountered problem faced in surgical practice. The presence of Dushta Vrana worsens the condition of the patient with different complications and may become fatal. Local factors on wound like slough, infection and foreign body, affect the normal process of healing. A healthy wound in a normal body heals earlier with a minimum scar as compared to a contaminated wound. Therefore in this study all the efforts are made to make a Dushta Vrana into a Shuddha Vrana. Once the Vrana becomes Shuddha, Ropana of the Vrana will start. The objective of the study was to evaluate the clinical efficacy of Jatyadi Taila and Jatyadi Ghrita in Dushta Vrana. Clinically diagnosed 60 Patients of Dushta Vrana were randomly divided into two groups, each consisting of 30 Patients. Group A were treated with the Jatyadi Taila and Group B was treated by Jatyadi Ghrita. The results observed was based on the relief obtained on the subjective and objective parameters taken for consideration for this study viz, size of ulcer, discharge, smell, pain, burning sensation, itching and granulation were found significant (P<0.05). On the basis of assessment criteria and overall result of treatment, the patients of Jatyadi Taila group showed better results when compared to Jatyadi Ghrita group. Even though statistically there is no much significant difference between the two groups, but by seeing the effect on individual parameters (subjective and objective) and over all response, Jatyadi Taila seems to be effective when compared to Jatyadi Ghrita. It is having more Ropana qualities when compared to Shodhana

Pathological and molecular characterizations of slow leaf rusting in fifteen wheat (Triticum aestivum L. em Thell) genotypes

Leaf rust caused by Puccinia triticina, is a globally important fungal disease of wheat (Triticum aestivum L. em. Thell), resulting in significant yield losses, sometimes up to 40% worldwide. In this study we investigated slow rusting resistance at pathological and molecular level. Fifteen (15) wheat genotypes which also included multiple crosses with the aim to characterize pyramid resistance genes, including slow rusting genes like Lr46 and Lr50 were evaluated for disease severity percent, latent period and incubation period under field conditions. Detached leaf assay was also performed with three virulent pathotypes viz., 21R55 (104-2), 121R63-1 (77-5) and 29R45 (12-5), under controlled laboratory conditions. Genotypes, KIRITATAI//HUW234+LR34/PRINIA, WAXWING*2/TUKURU, WBLLI*2/KIRITATI, KAMBI*2/-BRAMBLING and KAMBI*2/KIRITATI were very close to near immunity and showed comparatively higher level of resistance against all the three pathotypes. Disease severity in resistant genotypes was traced type 5 to 6% in both years, while it was 60 to 80% in the case of susceptible genotypes, that is, ‘Agra Local’ (S1). Similar pattern was observed for AUDPC, that is, <250.0 in the resistant genotypes, while it was beyond 1000.0 in ‘Agra Local’. The shorter mean latent (7.67) and incubation period (6.0) was observed in susceptible genotypes, that is, ‘Agra Local’ to all the resistant genotypes, that is, LP (10 to 12) and IP (9 to 10); while testing against all the three different pathotypes. Linked microsatellite markers were used to confirm the presence of different rust resistance genes required to achieve near immunity. Out of 10 primers, nine produced gene specific bands with all genotypes except the control, that is, Agra Local. Genotypes which showed slow rusting, had longer latent period and incubation period as well as reduced percent disease severity and confirmed the presence of four to five resistance genes including slow rusting genes, that is, Lr46 and Lr50. This indicates that these genotypes have potential durable resistance and can be used as parental lines in the development of more durable rust resistance.Key words: Near immunity, pathotypes, Puccinia triticina, SSR

Effect of Silver Addition on the Ethanol-Sensing Properties of Indium Oxide Nanoparticle Layers: Optical Absorption Study

In2O3 and In2O3:Ag nanoparticle layers have been deposited using a two-step method consisting of chemical capping and dip coating techniques. The result of optical absorption analysis of In2O3:Ag samples shows the presence of Ag2O and Ag in air-annealed and vacuum-annealed samples, respectively. These results have been correlated with the gas sensing properties of these layers towards ethanol and support the proposed mechanism that increase in sensor response on Ag addition is due to the conversion of Ag2O to Ag in the presence of ethanol

Influence of different organic sources of nutrients on growth and flowering behaviour of pomegranate (Punica granatum L.) cv. Bhagwa

A field experiment on the influence of different organic sources of nutrients on growth and flowering behaviour of pomegranate (Punica granatum L.) cv. Bhagwa was conducted at College Farm, College of Horticulture, Sardarkrushinagar Dantiwada Agricultural University, Jagudan, District-Mehsana in Gujarat, India during Mrig bahar (June-January)  2017-18 and 2018-19. The present investigation was laid out in Randomized Block Design with three replications consisting of twenty-two treatments with four different organic manures viz., farmyard manure, vermicompost, poultry manure and neem cake as a source of nitrogen and recommended dose of manure and fertilizers based on plant age with or without biofertilizers (Azotobacter, PSB & KMB) and biopesticides (Trichoderma viride and Paecilomyces lilacinus). The results based on pooled data revealed that a maximum number of hermaphrodite flowers (85.17) and incomplete flowers (96.50) up to two months after treatment application, fruit set (69.45 %) along with minimum fruit drop (13.18 %) and days taken for marketable picking (168.83) were significantly (@5%) noted under treatment 100 % RDN through poultry manure + 50 ml PSB + 25 ml KMB + 5 g Trichoderma viride + 5 ml Paecilomyces lilacinus. However, significantly (@5%) highest incremental primary growth parameters viz., plant height (0.60 m), plant spread (E-W: 0.41 m and N-S: 0.43 m) and stem girth (1.47 cm) after two months of treatment application were observed under treatment 100 % RDN through vermicompost + 50 ml PSB + 25 ml KMB + 5 g Trichoderma viride + 5 ml Paecilomyces lilacinus. Thus, the organic sources viz., vermicompost and poultry manure with biofertilizers and biopesticides would be very helpful for enhancing vegetative growth and flowering behaviour of the pomegranate

Dehydrocostus lactone from the root of Ajuga integrifolia (Buch.-Ham. Ex D. Don): Quantitative determination and in- silico study for anti-breast cancer activity

Many biological activities were reported for the Ajuga species, specifically for Ajuga integrifolia and its synonyms. These include antioxidant, anti-inflammatory, antidiabetic, antibacterial, blood purifier effects, and anticancer activity. This study quantitatively determines dehydrocostus lactone (DHCL) from the root of Ajuga integrifolia and its in silico study for anti-breast cancer activity. Camag HPTLC was used for TLC – densitometric estimation of dehydrocostus lactone. Estrogen receptor alpha (ER?) protein (PDB ID: 3ERT) was selected for its involvement in cell proliferation within the breast cancer cell. Tamoxifen is a reference drug commonly used in hormonal therapy, and DHCL was used as a ligand. Molecular docking was performed using AutoDock Vina in PyRx v.0.8 to get the bestconformational pose for forming the expected receptor-ligand complex. The docking result visualization was performed using LigPlot v.1.4.5 software for 2D, and the interactive visualization in 3D was done using Biovia Discovery Studio software. The presence of DHCL in the root of A. integrifolia was not reported so far. DHCL content in the root of A. integrifolia was estimated to be 16.5 ± 0.25 mg/g of crude extract using the TLC- densitometric method. From the molecular docking study, DHCL was found to be a promising inhibitor for estrogen receptor interaction in the breast cell and can be selected for further in vivo research to develop an anti-breast cancer drug