Prevalencia de periodontite juvenil localizada, generalizada e incipiente e presença de Actinobacillus actinomycetemcomitans em individuos de 15 a 25 anos de idade

Orientador: Antonio Olavo Cardoso JorgeTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: As periodontites que atingem indivíduos jovens podem ser classificadas em periodontite pré-pubertal, periodontite juvenil localizada ou generalizada e periodontite incipiente. O estudo da prevalência destas patologias apresenta extensa variação nos valores encontrados por diversos autores. O objetivo do presente estudo foi avaliar a condição clínica periodontal de indivíduos entre 15 e 25 anos de idade da região do Vale do Paraíba, estado de São Paulo, nos quais observou-se a prevalência de periodontite juvenil localizada, generalizada e incipiente e sua correlação com a presença de Actinobacillus actinomycetemcomitans. A condição periodontal dos indivíduos foi estabelecida através de procedimentos de sondagem e a presença de A. actinomycetemcomitans determinada através de cultura bacteriana. Para a análise dos dados foram empregados os testes Qui-quadrado e t de Student, e o nível de significância considerado foi p < 0,05. Dos 600 indivíduos analisados, com média de idade de 19,46::t 3,44 anos, 10 apresentaram periodontite juvenil localizada. Periodontite juvenil generalizada e incipiente foram observadas respectivamente em 22 e 86 indivíduos. Correlação positiva foi observada entre sexo feminino e as três formas de doença periodontal (x2 = 5,27 e p = 0,02). A. actinomycetemcomitans foi encontrado em 28 de 93 indivíduos com doença periodontal não havendo correlação entre a presença do patógeno e as variáveis analisadas. Através dos resultados observados no presente estudo pode-se verificar que a prevalência de periodontite juvenil localizada, generalizada e incipiente foram respectivamente 1,66, 3,66 e 14,3%. A. ctinomycetemcomitans foi encontrado em 60% dos indivíduos com periodontite juvenil localizada, 54,5% dos que apresentaram periodontite juvenil generalizada e em 16,4% dos que apresentaram periodontite incipienteAbstract: The periodontal diseases that affected young people have been classified in prepubertal, localized, generalized and incidental periodontitis. Epidemiological studies have been showed different data when these pathologies are studied. The aim of the present study was evaluated clinical conditions of 600 subjects between 15 - 25 years old from Vale do Paraíba, São Paulo to detect the prevalence of localized, generalized and incidental periodontitis and the presence of A. actinomycetemcomitans. The periodontal status was determined by periodontal probing and the presence of A. actinomycetemcomitans was observed by culture features. Chi-square and t Student were applied to verify the data (p < 0,05). The mean age of the population studied was 19,46 :t 3,44. Ten subjects showed localized juvenile periodontitis. Generalized juvenile periodontitis and incidental periodontitis were observed in 22 and 86 subjects respectively. Females showed a positive correlation with periodontal disease (,.l =5,27 e p =0,02). In 28 out of 93 subjects were found A. actinomycetemcomitans and a negative correlation was observed between this pathogen and other variables. In conclusion the prevalence of localized, generalized and incidental periodontitis were 1,66, 3,66 and 14,3% respectively and the presence of A. actinomycetemcomitans was found in 60% of localized juvenile periodontitis, 54,5% of generalized juvenile periodontits and 16,4% of incidental periodontitisDoutoradoDoutor em Biologia e Patologia Buco-Denta

Epidemiology of Periodontal Disease

848641Made available in DSpace on 2019-09-11T20:51:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2012[No abstract available]Costa, F.O., Federal University of Minas Gerais, Contorno Avenue 4849, 30110031 Belo Horizonte, MG, BrazilSusin, C., Laboratory for Applied Periodontal and Craniofacial Regeneration, College of Dental Medicine, Georgia Health Sciences University, United StatesCortelli, J.R., University of Taubaté, Taubaté, SP, BrazilAlmeida Pordeus, I., Federal University of Minas Gerais, Contorno Avenue 4849, 30110031 Belo Horizonte, MG, Brazi

Identification of genetic variants associated with Huntington's disease progression: a genome-wide association study

Background Huntington's disease is caused by a CAG repeat expansion in the huntingtin gene, HTT. Age at onset has been used as a quantitative phenotype in genetic analysis looking for Huntington's disease modifiers, but is hard to define and not always available. Therefore, we aimed to generate a novel measure of disease progression and to identify genetic markers associated with this progression measure. Methods We generated a progression score on the basis of principal component analysis of prospectively acquired longitudinal changes in motor, cognitive, and imaging measures in the 218 indivduals in the TRACK-HD cohort of Huntington's disease gene mutation carriers (data collected 2008–11). We generated a parallel progression score using data from 1773 previously genotyped participants from the European Huntington's Disease Network REGISTRY study of Huntington's disease mutation carriers (data collected 2003–13). We did a genome-wide association analyses in terms of progression for 216 TRACK-HD participants and 1773 REGISTRY participants, then a meta-analysis of these results was undertaken. Findings Longitudinal motor, cognitive, and imaging scores were correlated with each other in TRACK-HD participants, justifying use of a single, cross-domain measure of disease progression in both studies. The TRACK-HD and REGISTRY progression measures were correlated with each other (r=0·674), and with age at onset (TRACK-HD, r=0·315; REGISTRY, r=0·234). The meta-analysis of progression in TRACK-HD and REGISTRY gave a genome-wide significant signal (p=1·12 × 10−10) on chromosome 5 spanning three genes: MSH3, DHFR, and MTRNR2L2. The genes in this locus were associated with progression in TRACK-HD (MSH3 p=2·94 × 10−8 DHFR p=8·37 × 10−7 MTRNR2L2 p=2·15 × 10−9) and to a lesser extent in REGISTRY (MSH3 p=9·36 × 10−4 DHFR p=8·45 × 10−4 MTRNR2L2 p=1·20 × 10−3). The lead single nucleotide polymorphism (SNP) in TRACK-HD (rs557874766) was genome-wide significant in the meta-analysis (p=1·58 × 10−8), and encodes an aminoacid change (Pro67Ala) in MSH3. In TRACK-HD, each copy of the minor allele at this SNP was associated with a 0·4 units per year (95% CI 0·16–0·66) reduction in the rate of change of the Unified Huntington's Disease Rating Scale (UHDRS) Total Motor Score, and a reduction of 0·12 units per year (95% CI 0·06–0·18) in the rate of change of UHDRS Total Functional Capacity score. These associations remained significant after adjusting for age of onset. Interpretation The multidomain progression measure in TRACK-HD was associated with a functional variant that was genome-wide significant in our meta-analysis. The association in only 216 participants implies that the progression measure is a sensitive reflection of disease burden, that the effect size at this locus is large, or both. Knockout of Msh3 reduces somatic expansion in Huntington's disease mouse models, suggesting this mechanism as an area for future therapeutic investigation

Human beta-defensin 2 and protease activated receptor-2 expression in patients with chronic periodontitis

Objective: Some previous studies have shown that gingipains, trypsin-like proteases produced by Porphyromonas gingivalis, up-regulate human beta defensin-2 (HBD-2) mRNA expression through protease-activated receptor-2 (PAR(2)) in gingival epithelial cells. This study aimed at investigating salivary HBD-2 levels and crevicular PAR(2) mRNA expression in human chronic periodontitis and evaluating whether periodontal treatment affected this process. Methods: Salivary and gingival crevicular fluid (GCF) samples were collected from periodontally healthy (control) and chronic periodontitis patients at baseline and 50 days after nonsurgical periodontal treatment. Salivary HBD-2, and GCF TNF-alpha levels were analysed by ELISA, and PAR(2) mRNA at the GCF was evaluated by RT-PCR. Results: P. gingivalis was significantly (p &lt; 0.05) more prevalent in patients with chronic periodontitis when compared to controls. This prevalence decreased after periodontal therapy (p &lt; 0.0001). The control group showed statistically significant lower levels of HBD-2, TNF-alpha, and PAR(2) expression when compared to the chronic periodontitis group. In addition, periodontal treatment significantly reduced PAR(2) expression and HBD-2 levels in chronic periodontitis patients (p &lt; 0.001). Conclusions: Our results suggest that salivary HBD-2 levels and PAR(2) mRNA expression from GCF are higher in subjects with chronic periodontitis than in healthy subjects, and that periodontal treatment decreases both HBD-2 levels and PAR(2) expression. (C) 2012 Elsevier Ltd. All rights reserved.State University of Sao Paulo Research Foundation, Sao Paulo, Brazil (FAPESP) [07/50665-8]State University of Sao Paulo Research Foundation, Sao Paulo, Brazil (FAPESP

Association between severity of body mass index and periodontal condition in women

Made available in DSpace on 2019-09-12T16:53:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2012Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)This study evaluated the association between body mass index (BMI) and periodontal condition in a population of Brazilian women. A hospital convenience sample of 594 eligible women was recruited from a women's health reference center of Belo Horizonte, Brazil. Four groups were formed considering BMI levels: BMI normal group ( = 352), overweight ( = 54), obesity level I ( = 48), obesity level II ( = 56), and obesity level III ( = 74). Full-mouth periodontal examination was performed and biological, demographic, and behavioral risk variables were evaluated. Obese and overweight women showed statistically significant differences in bleeding on probing, probing depth and clinical attachment level a parts per thousand yen4 mm, and frequency of periodontitis ( < 0.05) compared to women showing normal BMI. The final multivariate model for the occurrence of periodontitis revealed that obesity groups were significantly associated with periodontitis. In addition, age (25-45), smoking, diabetes, and hypertension remained significantly associated with the occurrence of periodontitis ( < 0.05). Periodontitis was positively associated with obesity, and this association was more evident as obesity levels increases. These findings indicate the need for early diagnosis and the inclusion of periodontal care in health care programs for obese women.[Pataro, Andre Luiz; Costa, Fernando Oliveira; Costa, Jose Eustaquio] Univ Fed Minas Gerais, Sch Dent, Dept Periodontol, BR-31270901 Belo Horizonte, MG, Brazil[Cortelli, Sheila Cavalca; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Dept Dent, Periodont Res Div, Sao Paulo, Brazil[Nogueira Guimaraes Abreu, Mauro Henrique] Univ Fed Minas Gerais, Dept Social & Prevent Dent, BR-31270901 Belo Horizonte, MG, Brazi

Prospective Study in Periodontal Maintenance Therapy: Comparative Analysis Between Academic and Private Practices

Made available in DSpace on 2019-09-12T16:56:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2012Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Background: This prospective study aims to evaluate and compare the periodontal status, periodontitis progression, tooth loss, and influence of predictable risk variables of two periodontal maintenance therapy programs over a 12-month period. Methods: A total of 288 individuals diagnosed with chronic moderate-to-advanced periodontitis, who had finished active periodontal treatment, were evaluated in a public academic environment (AG) (n = 138), as well as in a private clinic (PG) (n = 150). A full-mouth periodontal examination was performed at baseline and at quadrimestral recalls, evaluating plaque index, probing depth, clinical attachment level, furcation involvement, bleeding on probing (BOP), and suppuration. Individuals' social, demographic, and biologic data, as well as compliance with recalls, were recorded. The effect of variables of interest and confounders were tested by univariate and multivariate analysis. Results: The PG demonstrated lower rates of periodontitis progression and tooth loss than did the AG. After adjusting for confounders, the risk variables of BOP (P=0.047), smoking (P = 0.003), and diabetes (P = 0.028) for the PG and smoking (P=0.047) for the AG showed a negative influence on periodontal status. Conclusions: In both groups, the periodontal maintenance therapy minimized the negative effect of the risk variables. However, PG showed significantly less progression of periodontitis and tooth loss compared to AG. J Periodontol 2012;83:301-311.[Costa, Fernando Oliveira; Santuchi, Camila Carvalho; Pereira Lages, Eugenio Jose; Miranda Cota, Luis Otavio; Medeiros Lorentz, Telma Campos; Costa, Jose Eustaquio] Univ Fed Minas Gerais, Dept Periodontol, Sch Dent, BR-31270901 Belo Horizonte, MG, Brazil[Cortelli, Sheila Cavalca; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Dept Dent, Periodont Res Div, Sao Paulo, Brazi

Oral Impact on Daily Performance, Personality Traits, and Compliance in Periodontal Maintenance Therapy

Made available in DSpace on 2019-09-12T16:56:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2011Fundação de Amparo a Pesquisa do Estado de Minas Gerais (Fapemig) (FAPEMIG-Brazil) [10137]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Background: To our knowledge, the association between personality traits and oral health impacts on daily activities among patients under periodontal maintenance was not previously evaluated. The present study aims to determine the oral health impacts among regular- and erratic-complier patients and their correlations with personality traits by means of the oral impacts on daily performance (OIDP) and the neuroticism extraversion openness five-factor inventory (NEO FFI-R) instruments. Methods: A total of 58 regular and 58 erratic compliers matched by sex, diabetes, and smoking were recruited from a cohort of 238 participants under periodontal maintenance. Periodontal examinations and questionnaire applications were performed in recalls during a 3-year interval. The influence of variables of interest on the OIDP was evaluated through univariate and multivariate linear regression analyses. Results: The periodontal status of regular compliers was significantly better than that of erratic compliers. Erratic compliers presented higher OIDP scores (36.34 +/- 6.64) compared to regular compliers (30.45 +/- 7.72). Higher scores of neuroticism and conscientiousness (R(2) = 68%; P <0.001) were associated with higher OIDP scores among regular compliers, whereas among erratic compliers, lower scores of neuroticism, higher openness, and extraversion (R(2) = 77%; P = 0.001) were associated with higher OIDP scores. Conclusion: Personality traits, mainly neuroticism, showed significant influences on OIDP among patients under periodontal maintenance. J Periodontol 2011;82:1146-1154.[Costa, Fernando Oliveira; Miranda Cota, Luis Otavio; Pereira Lages, Eugenio Jose; Costa, Jose Eustaquio; Medeiros Lorentz, Telma Campos] Univ Fed Minas Gerais, Dept Periodontol, Sch Dent, BR-31270901 Belo Horizonte, MG, Brazil[Vilela Camara, Gislene Clemente] Pontifical Univ Catholic Minas Gerais, Inst Psychol, Belo Horizonte, MG, Brazil[Cortelli, Sheila Cavalca; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Periodont Res Div, Dept Den

Periodontal Risk Assessment Model in a Sample of Regular and Irregular Compliers Under Maintenance Therapy: A 3-Year Prospective Study

Made available in DSpace on 2019-09-12T16:56:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2012Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Background: The purpose of this study is to investigate the association of the periodontal risk assessment (PRA) model with the recurrence of periodontitis and tooth loss during periodontal maintenance therapy (PMT). Methods: In a prospective PMT program, 75 regular complier (RC) and 89 erratic complier (EC) patients were selected. A periodontal examination and PRA were performed after active periodontal therapy and after 3 years of PMT. Risk profiles (low, moderate, or high) of participants were evaluated, and the recurrence of periodontitis and tooth loss were analyzed using univariate and multivariate analyses. Results: RCs showed less recurrence of periodontitis and tooth loss than ECs (P<0.05). Rates of periodontitis recurrence in RCs and ECs were 2.7% and 3.4%, respectively, for the moderate-risk profile and 6.7% and 11.2%, respectively, for the high-risk profile. During PMT, 49 teeth (0.65 +/- 1.4 teeth per participant) were lost in the RC group, and 70 teeth (0.78 +/- 2.1 teeth per participant) were lost in the EC group. High-risk profile participants showed more recurrence of periodontitis and lost significantly more teeth than did participants with moderate- or low-risk profiles in RC and EC groups (P<0.05). Conclusion: The risk profile influenced the recurrence of periodontitis and tooth loss. RCs had less recurrence of periodontitis and less tooth loss. The PRA model can be useful in particularizing the risk of patients and adjusting recall intervals. J Periodontol 2012;83:292-300.[Costa, Fernando Oliveira; Miranda Cota, Luis Otavio; Pereira Lages, Eugenio Jose; Lima Oliveira, Ana Paula; Medeiros Lorentz, Telma Campos; Costa, Jose Eustaquio] Univ Fed Minas Gerais, Dept Periodontol, Sch Dent, BR-31270901 Belo Horizonte, MG, Brazil[Cortelli, Sheila Cavalca; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Periodont Res Div, Dept Den

Porphyromonas Gingivalis is Associated With Protease-Activated Receptor-2 Upregulation in Chronic Periodontitis

Made available in DSpace on 2019-09-12T16:56:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2011Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade de Taubaté (Unitau)Background: We previously reported a higher expression of protease-activated receptor-2 (PAR(2)) together with higher interleukin (IL)-1 alpha, IL-6, IL-8, and tumor necrosis factor-alpha levels, total proteolytic activity, Porphyromonas gingivalis (Pg) prevalence, and neutrophil-protease 3 messenger RNA (mRNA) expression in patients with chronic periodontitis compared to healthy control patients. The aim of the present study is to expand this observation by considering the site level according to the presence of Pg. Methods: Microbiologic and gingival crevicular fluid samples were collected from patients with chronic periodontitis. Pg presence was evaluated by polymerase chain reaction and PAR(2) mRNA expression was evaluated by reverse-transcription polymerase chain reaction. Total proteolytic activity in the crevicular fluid was analyzed by using a specific substrate benzoylarginine nitroanilide, and the proinflammatory mediators IL-1 alpha, IL-6, IL-8, and tumor necrosis factor-alpha were analyzed by enzyme-linked immunosorbent assay. Results: In Pg-positive periodontal sites, the mean probing depth and clinical attachment level, the prevalence of bleeding on probing sites, and crevicular fluid volume were higher (P < 0.05) compared to Pg-negative sites. In addition, with the exception of IL-8, all other inflammatory mediators were positively (P < 0.05) associated with Pg presence. Pg presence was also positively associated with a higher proteolytic activity (P = 0.0037) and higher PAR(2) mRNA expression (P = 0.0271). Conclusions: We conclude that in chronic periodontitis, periodontal pockets presenting Pg show an upregulation of PAR(2) gene expression, and higher proinflammatory profile associated with advanced clinical destruction, therefore suggesting that Pg plays a pivotal role on PAR(2)-mediated periodontal inflammation in humans. J Periodontol 2011;82:1596-1601.[Fagundes, Jose Americo G.; Monoo, Leticia D.; Cortelli, Sheila Cavalca; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Dept Periodontol, Sao Paulo, Brazil[Euzebio Alves, Vanessa Tubero; Pannuti, Claudio Mendes; Holzhausen, Marinella] Univ Sao Paulo, Div Periodont, Dept Stomatol, Sch Dent, Sao Paulo, Brazi