Heterologous expression of AtNPR1 gene in olive for increasing fungal tolerance

The NPR1 gene encodes a key component of SAR signaling mediated by salicylic acid (SA). After a pathogen infection, the accumulation of SA releases NPR1 monomers in the cytosol that are translocated to the nucleus, activating the expression of pathogenesis-related (PR) genes. Overexpression of NPR1 has conferred resistance to fungal, viral and bacterial pathogens in several plant species. The aim of this research was to generate transgenic olive plants expressing the gene AtNPR1 from Arabidopsis thaliana to obtain material resistant to fungal pathogens. Three transgenic lines expressing AtNPR1 gene under the control of the constitutive promoter CaMV35S were obtained following the protocol of Torreblanca et al. (2010), using an embryogenic line derived from a seed of cv. Picual. Level of AtNPR1 expression in transgenic calli varied greatly among the different lines, being higher in the line NPR1-780. The elicitation of embryogenic calli in liquid medium with AS did not increase endochitinase activity, a PR protein. However, jasmonic acid induced a transient increase in chitinase activity after 24 h of treatment in all the lines, being the increment higher in transgenic NPR1 than in control. After maturation and germination of transgenic somatic embryos, plants were micropropagated and acclimated to ex vitro conditions. The expression of AtNPR1 did not alter the growth of transgenic plants neither in vitro nor in the greenhouse. Experiments are in progress to determine the resistance of transgenic AtNPR1 plants to V. dalihae and R. necatrix.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Research projects: Plan Nacional AGL2014-52518-C2-1-R; AGL2017-83368-C2-1-R and Junta de Andalucía P11-AGR799

Teaching Style in Physical Education and Changes of Daily Physical Activity after One Academic Year in Adolescents: GEOS Study

Increased Moderate-Vigorous physical activity (MVPA) and reduced sedentary time (ST) are key factors for a healthy lifestyle during childhood and adolescence. Studies have suggested that schools may be effective resources to promote healthy habits (Sallis, McKenzie et al. 2012). Therefore, in PE, is important to assess how teachers use strategies and provide students tools to engage in PA with the purpose of reduce the risk of sedentary behavior and contribute to promotion MVPA habits for a healthy lifestyle (Lonsdale, C. et al., 2013). Many factors may be involved in the successful PE class to promote healthy out-school behaviors, as teaching styles (TS), learning styles, learning time, motivation and so on (Mosston, M. 1966). Regarding TS, there is a lack of knowledge about influence of the teaching style (TS) in the promotion of daily MVPA. It was our aim to observe the differences of total daily PA dimensions between two groups of adolescents who were taught during a whole academic year using reproducing (RK) or producing knowledge (PK) TSs.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Immobilization of Pyrene-Tagged Palladium and Ruthenium Complexes onto Reduced Graphene Oxide: An Efficient and Highly Recyclable Catalyst for Hydrodefluorinatio

The co-immobilization of palladium and ruthenium complexes with pyrene-tagged Nheterocyclic carbene ligands onto reduced grahene oxide allows the formation of a highly efficient catalyst for the hydrodefluorination of a series of fluoroarenes. This procedure constitutes an easy one-pot preparation of materials with homogeneously distributed polymetallic catalysts. The catalytic system can be recycled for up to twelve times without measurable loss of activity. The activity of the catalyst is attributed to the synergistic action of the two metals.Ministerio de Ciencia e Innovación of Spain (CTQ2011- 24055/BQU)

Personalised and Adjustable Interval Type-2 Fuzzy-Based PPG Quality Assessment for the Edge

Most of today's wearable technology provides seamless cardiac activity monitoring. Specifically, the vast majority employ Photoplethysmography (PPG) sensors to acquire blood volume pulse information, which is further analysed to extract useful and physiologically related features. Nevertheless, PPG-based signal reliability presents different challenges that strongly affect such data processing. This is mainly related to the fact of PPG morphological wave distortion due to motion artefacts, which can lead to erroneous interpretation of the extracted cardiac-related features. On this basis, in this paper, we propose a novel personalised and adjustable Interval Type-2 Fuzzy Logic System (IT2FLS) for assessing the quality of PPG signals. The proposed system employs a personalised approach to adapt the IT2FLS parameters to the unique characteristics of each individual's PPG signals.Additionally, the system provides adjustable levels of personalisation, allowing healthcare providers to adjust the system to meet specific requirements for different applications. The proposed system obtained up to 93.72\% for average accuracy during validation. The presented system has the potential to enable ultra-low complexity and real-time PPG quality assessment, improving the accuracy and reliability of PPG-based health monitoring systems at the edge

Steganography and watermarking on BMP images

In this work we perform some tests of steganography and watermarking on BMP images. We use the Discrete Cosine Transform (DCT) and Wavelets in order to insert information into high and medium frequencies. When steganography is used, we explain a method how to introduce secret data into an image and we show the capacity of the image to accept these data. For the watermarking technique we indicate where the data should be placed in order to achieve a robust insertion of the data even in the presence of image compression. Finally, we make a comparison between these two techniques

Impact on the Hipparcos2-UCAC4 geometric relation from some physical properties of the stars

[EN] The aim of this paper is the study of the impact that the consideration of different physical properties as magnitude and spectral type of stars has on the geometric relations between Hipparcos2 and UCAC4. In this sense, the pairs of residuals ¿¿ ¿ and ¿¿ can be considered as functions of (¿, ¿, r) and for each fixed r, we can fit a vector field on the sphere from which to obtain its components in the VSH basis. The same can be done by grouping the stars considering their magnitudes, spectral types (or mixing them) and then studying the variations in the mentioned geometry. We must not forget that ¿¿ ¿ and ¿¿ are numerical random variables whose regression on the magnitude m, for example, can be estimated. The results will be computed taking into account r as well as the physical mentionThis work was supported by a grant UJI-B2016-18Marco, FJ.; Martínez Uso, MJ.; López, JA. (2018). Impact on the Hipparcos2-UCAC4 geometric relation from some physical properties of the stars. Proceedings of the International Astronomical Union. 12(S330):237-238. https://doi.org/10.1017/S1743921317006457S23723812S330Marco F. J. , Martínez M. J. & López J. A. 2017, Astrometry and Astrophysics in the Gaia Sky, In Press

A 3D-Study of the residual vector field

[EN] One of the important challenges that Gaia imposes on the Astrometric Catalogs, is a careful study in everything affected by parallax. A particularly important case is the necessary linkage Gaia - HCRF - ICRF2, which require methods of analysis that are accurate enough so that the provided results are at the same precision level as the work data.This work was supported by a grant UJI-B2016-18Marco, FJ.; Martínez Uso, MJ.; López, JA. (2018). A 3D-Study of the residual vector field. Proceedings of the International Astronomical Union. 12(S330):235-236. https://doi.org/10.1017/S1743921317006536S23523612S33

Use of a PSNM to Increase Precocity and Its Benefits in Greenhouse-Grown Sweet Pepper

The types and varieties of peppers grown in Mediterranean areas are a response to the demand of European markets, although in each Autonomous Community local varieties are grown to satisfy the national demand. Nowadays, the range of shapes, colours, tastes and uses is wider than ever as a result of greenhouse cultivation, national and international tendencies and increased demand. In Murcia, the growing cycle runs from December to July or August, depending on the market and the growth of the crop. Sweet pepper is normally grown in greenhouses, using a variety of technologies: from simple shaded greenhouses, to the most-advanced multitunnels (large, in the form of a round arch or Gothic arch and with sophisticated ventilation). Due to the high cost of fuel, it is impossible to use heating during winter after transplanting, so alternative techniques are used to raise the temperature a few degrees and improve crop production. The aim of this work was to increase the precocity and productivity of sweet pepper grown in greenhouses. The effect of a Polypropylene Spunbonded Nonwoven Microtunnel (PSNM) was studied. The results show that, although the increase in production was not great (lower than 5% in both years of the study), precocity increased by 16% in both years. Since the increased cost of using this technology is not excessive, crop profitability increases if precocity is taken into account, as all our indicators show. The study suggests that the use of a PSNM raises the marketable production and brings forward the first harvests

Enhancement of gold catalytic activity and stability by immobilization on the surface of graphene

The catalytic performance of gold complexes is evaluated at the molecular level and when supported onto reduced graphene oxide (rGO). Gold complexes of general formula [(NHC)AuX] catalyse the synthesis of indoles via intramolecular hydroamination reaction of alkynes. The catalytic properties of the molecular gold complexes are highly improved when supported onto graphene. Faster reaction rates and higher catalyst stability are observed for the immobilized gold complexes. The use of graphene as support of molecular complexes has a positive benefit in the catalytic gold properties in terms of activity and stabilityThe authors thank the financial support from MINECO (CTQ2015-69153-C2-2-R), Generalitat Valenciana (AICO/2015/039) and Universitat Jaume I (P1.1B2015-09). D. V-E thanks MINECO for a FPU grant (FPU15/03011