Confirmacion del informe dado en 26 de abril del presente año 1735 por Thomas Comes ... y Doña Theresa Meseguer, viuda, y heredera de Don Francisco Comes ... en el pleyto con ... Pedro Juan Comes ... Francisco Comes ... y Esperanza Comes, hijos de Migue

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Memorial ajustado del pleyto que en este S.S.R.C. de Aragon pende por letras causavidendi, & recognocendi, entre ... Ines Maria de Palafox, Condesa de Buñol, como madre ... del Egregio Conde de Buñol, y Cerbellò D. Franco Maria Pasqual Mercader y Cerbe

A 109/113(14

Separation of Isomeric Forms of Urolithin Glucuronides Using Supercritical Fluid Chromatography

Urolithins are gut microbiota metabolites produced in humans after consuming foods containing ellagitannins and ellagic acid. Three urolithin metabotypes have been reported for different individuals depending on the final urolithins produced. After absorption, they are conjugated with glucuronic acid (phase II metabolism), and these are the main circulating metabolites in plasma and reach different tissues. Different regioisomeric isomers of urolithin glucuronides have been described. Still, their identification and quantification in humans have not been properly reported due to resolution limitations in their analysis by reversed-phase high-performance liquid chromatography. In the present study, we report a novel method for separating these isomers using supercritical fluid chromatography. With this method, urolithin A 3- and 8-glucuronide, isourolithin A 3- and 9- glucuronide, and urolithin B 3-glucuronide (8-hydroxy urolithin 3-glucuronide; 3-hydroxy urolithin 8-glucuronide; 3-hydroxyurolithin 9-glucuronide; 9-hydroxyurolithin 3-glucuronide; and urolithin 3-glucuronide) were separated in less than 15 min. The proposed method was applied to successfully analyze these metabolites in urine samples from different volunteers belonging to different metabotypes

Magnetite Nanoparticles for Stem Cell Labeling with High Efficiency and Long-Term in Vivo Tracking

Superparamagnetic iron oxide nanoparticles (<b>SPIO-PAA</b>), ultrasmall iron oxide nanoparticles (<b>USPIO-PAA</b>), and glucosamine-modified iron oxide nanoparticles (<b>USPIO-PAA-GlcN</b>) were studied as mesenchymal stem cell (MSCs) labels for cell tracking applications by magnetic resonance imaging (MRI). Pronounced differences were found in the labeling performance of the three samples in terms of cellular dose and labeling efficiency. In combination with polylysine, <b>SPIO-PAA</b> showed nonhomogeneous cell internalization, while for <b>USPIO-PAA</b> no uptake was found. On the contrary, <b>USPIO-PAA-GlcN</b> featured high cellular uptake and biocompatibility, and sensitive detection in both in vitro and in vivo experiments was found by MRI, showing that glucosamine functionalization can be an efficient strategy to increase cell uptake of ultrasmall iron oxide nanoparticles by MSCs

New Advances in the One-Dimensional Coordination Polymer Copper(II) Alkanoates Series: Monotropic Polymorphism and Mesomorphism

The polymorphism in the copper­(II) alkanoates, recently discovered for one member, has been thoroughly studied for the whole series, from 3 to 16 C atoms. Three polymorphic phases have been found, all of them sharing the same molecular unit, the <i>paddle-wheel</i>, which grows forming a 1D coordination polymer or <i>catena</i>. The three polymorphs are defined by a different packing of these catenae and a specific arrangement of the alkyl chains. Ten new crystal structures of those compounds have been solved by high resolution powder diffraction and presented in this paper. The polymorphism in this series has been found to be monotropic and is responsible for the complex thermal behavior observed. The most characteristic feature, the endothermic–exothermic effect, has been explained for the first time in these compounds by a combination of data from differential scanning calorimetry (in normal and modulated modes), powder X-ray diffraction and Fourier transform infrared spectroscopy. These techniques, together with small-angle X-ray scattering and optical microscopy, were used to analyze the hexagonal columnar discotic liquid crystal phase of copper­(II) alkanoates. Thus, new information has been found in the packing and stacking of the discs formed by the paddle-wheel units, also maintained in the mesophase

A Dietary Resveratrol-Rich Grape Extract Prevents the Developing of Atherosclerotic Lesions in the Aorta of Pigs Fed an Atherogenic Diet

The presence of grape and wine polyphenol resveratrol (RES) in the diet is negligible. Therefore, the cardiovascular benefits of this molecule, in a dietary context, remain to be established. We aimed to investigate, through dietary intervention, the effects of a resveratrol-rich grape extract (GE-RES) on the prevention of early aortic lesions in pigs fed an atherogenic diet (AD). These effects were compared with those produced by a grape extract lacking RES (GE) or RES alone. Pigs fed the AD for 4 months showed early atherosclerotic lesions in the thoracic aorta: degeneration and fragmentation of elastic fibers, increase of intima thickness, subendothelial fibrosis, and accumulation of fatty cells and anion superoxide radicals. GE-RES was the most effective treatment and prevented the disruption of aortic elastic fibers, decreased their alteration (57%), and reduced the intima thickness (33%) and the accumulation of fatty cells (42%) and O₂^•– (38%) in aortic tissue. In addition, GE-RES moderately downregulated the expression of the suppressors of cytokine signaling 1 (<i>SOCS1</i>) and 3 (<i>SOCS3</i>), key regulators of vascular cell responses, in peripheral mononuclear blood cells. Our results suggest that the consumption of this GE-RES nutraceutical, in a dietary prevention context, could prevent early atherosclerotic events. The presence of RES in the grape extract strengthened these effects