Autophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barley

Microspores are reprogrammed towards embryogenesis by stress. Many microspores die after this stress, limiting the efficiency of microspore embryogenesis. Autophagy is a degradation pathway that plays critical roles in stress response and cell death. In animals, cathepsins have an integral role in autophagy by degrading autophagic material; less is known in plants. Plant cathepsins are papain-like C1A cysteine proteases involved in many physiological processes, including programmed cell death. We have analysed the involvement of autophagy in cell death, in relation to cathepsin activation, during stress-induced microspore embryogenesis in Hordeum vulgare. After stress, reactive oxygen species (ROS) and cell death increased and autophagy was activated, including HvATG5 and HvATG6 up-regulation and increase of ATG5, ATG8, and autophagosomes. Concomitantly, cathepsin L/F-, B-, and H-like activities were induced, cathepsin-like genes HvPap-1 and HvPap-6 were up-regulated, and HvPap-1, HvPap-6, and HvPap-19 proteins increased and localized in the cytoplasm, resembling autophagy structures. Inhibitors of autophagy and cysteine proteases reduced cell death and promoted embryogenesis. The findings reveal a role for autophagy in stress-induced cell death during microspore embryogenesis, and the participation of cathepsins. Similar patterns of activation, expression, and localization suggest a possible connection between cathepsins and autophagy. The results open up new possibilities to enhance microspore embryogenesis efficiency with autophagy and/or cysteine protease modulators.España, MINECO AGL2014-52028-R and AGL2017-82447-

Eficacia de los programas de desarrollo socioemocional aplicados en educación primaria: Una revisión sistemática paraguas

En la actualidad ha aumentado el desarrollo de programas de entrenamiento en habilidades socioemocionales en la infancia, debido a que se los considera como una herramienta válida para la adaptación y afrontamiento de una gran variedad de situaciones, tanto académicas como personales. Sin embargo, son escasos los estudios que aporten una visión integral de las evidencias disponibles en el contexto de educación primaria. Se presenta una revisión sistemática de tipo paraguas basada en el método PRISMA, que incluye revisiones sobre programas de desarrollo socioemocional aplicados en educación primaria, con el objetivo de sintetizar sus características y recopilar los principales resultados reportados. Se utilizaron las bases de datos: ERIC, WOS, PSYCINFO, SCOPUS y COCHRANE. Tras un proceso por pares ciegos se seleccionaron y analizaron 15 revisiones. Utilizando las herramientas AMSTAR-2 y SANRA se encontró que el 60% de los estudios secundarios presenta una calidad críticamente baja o baja. Se identificaron 39 programas reportados en revisiones de buena calidad, un 51.2% presentaron evidencias moderadas o fuertes e informaron efectos significativos principalmente en ajuste del comportamiento, competencia social y emocional y habilidades académicas. Se discute el impacto de la calidad metodológica encontrada y las evidencias reportados en la interpretación y generalización de los hallazgosCurrently, the development of training programs in socioemotional skills in childhood has increased because they are considered as a valid tool for adaptation and coping with a variety of situations, both academic and personal. However, there are few studies that show a comprehensive view of available evidences. This research presents an umbrella review based on PRISMA method guidelines. It includes reviews on socioemotional development programs applied in Primary Education with the aim of synthesizing their characteristics and compiling the main results on their effectiveness. The following databases were used: ERIC, WOS, PSYCINFO, SCOPUS and COCHRANE. After a blind peer process, 15 reviews that met the inclusion criteria were selected and analysed. Using the AMSTAR-2 and SANRA tools, it was found that 60% of secondary studies have critically low or low quality. Thirty nine programs reported in good quality reviews were identified, 51.2% presented moderate or strong evidence and reported significant effectsmainly on behavioral adjustment, social and emotional competencies and academic skills. The impact of the methodological quality found and the evidences on the interpretation and generalization of the findings is discussed

Phosphorus Availability Regulates TORC1 Signaling via LST8 in Chlamydomonas

Target of rapamycin complex 1 (TORC1) is a central regulator of cell growth. It balances anabolic and catabolic processes in response to nutrients, growth factors, and energy availability. Nitrogen- and carbon-containing metabolites have been shown to activate TORC1 in yeast, animals, and plants. Here, we show that phosphorus (P) regulates TORC1 signaling in the model green alga Chlamydomonas (Chlamydomonas reinhardtii) via LST8, a conserved TORC1 subunit that interacts with the kinase domain of TOR. P starvation results in a sharp decrease in LST8 abundance and downregulation of TORC1 activity. A hypomorphic lst8 mutation resulted in decreased LST8 abundance, and it both reduced TORC1 signaling and altered the cellular response to P starvation. Additionally, we found that LST8 levels and TORC1 activity were not properly regulated in a mutant defective in the transcription factor PSR1, which is the major mediator of P deprivation responses in Chlamydomonas. Unlike wild-type cells, the psr1 mutant failed to downregulate LST8 abundance and TORC1 activity when under P limitation. These results identify PSR1 as an upstream regulator of TORC1 and demonstrate that TORC1 is a key component in P signaling in Chlamydomonas.España Ministerio de Economía y Competitividad (grants BFU2015-68216-P and PGC2018-099048- B-100 to J.L.C. and grant BIO2015-74432-JIN to M.E.P.-P.)National Science Foundation (CAREER award MCB-1552522 to L.M.H. and grant MCB-1616820 to J.G.U.)European Commission (grant number 750996

Desalination and water security in Southeastern Spain

The Segura Hydrographic Demarcation (DHS), in Southeastern Spain, is an area of hydric deficit caused by low and irregular rainfall and a dense population. In this region water scarcity is a burning issue that polarizes society for or against different models of ensuring water supply. Given the current demand for water throughout Spain, desalination has been used to increase water supply, and as insurance against drought. Ten seawater desalination plants and hundreds of brackish aquifer desalination plants treat water in the Southeast of the Iberian Peninsula, both for human consumption and for irrigation, contributing to economic, energetic, processual and economic aspects of territorial organization in the Southeast of Spain. This article reviews debates over desalination, water transfers, and the best way to meet water demands in that region. While desalination allows an increase in water supply, dependence on that source increases energy costs and may lead to mistaken assumptions among users about water scarcity and availabilityLa Demarcación Hidrográfica del Segura (DHS) comprende un área hídricamente deficitaria motivada por una reducida e irregular pluviometría y una intensa ocupación del territorio, en la que el recurso agua ha sido y es un bien limitado y muy preciado desde la antigüedad, y es un candente asunto de la actualidad que polariza a la sociedad a favor o en contra de un modelo u otro de abastecimiento. Ante las actuales demandas de este recurso, se hace necesario recurrir a la desalación para un incremento de la oferta hídrica que actúa como un seguro ante situaciones de sequía en España. Un total de diez plantas desaladoras y cientos de desalobradoras suministran agua desalada procedente del mar o de acuíferos salobres en el Sureste de la Península Ibérica, tanto para consumo humano como para la irrigación de los fértiles campos, aportando su idiosincrasia económica, energética, procesual y de ordenación territoria

Identification of Key Molecules Involved in the Protection of Vultures Against Pathogens and Toxins

This is an open access article distributed under the terms of the Creative Commons Attribution License.This work was supported by the Junta de Comunidades de Castilla-La Mancha (JCCM), project PII1I09-0243-4350.Peer Reviewe

A Molecular Counterpart to the Herbig-Haro 1-2 Flow

We present high angular resolution (12"-24") and high sensitivity 12CO and 13CO J=2-1 and J=1-0 observations of the HH 1-2 outflow. The observations show the molecular counterpart, moving with a velocity of approx. 30 km/s, of the optical bipolar system driven by the VLA 1 embedded source. Along the optical jet there are certain regions where the molecular gas reaches deprojected velocities of 100-200 km/s, and that we interpret as the molecular jet. The bipolar CO outflow has a length of approx. 260" with a curved morphology towards the North where it extends beyond the HH 1 object (approx. 120") . Two new molecular outflows have been detected, one arising from IRAS 05339-0647 which excites the HH 147 optical flow and another powered by VLA 2 which drives the HH 144 optical outflow. The molecular outflow driven by the VLA 3 source is also clearly detected and spatially resolved from the VLA 1 main outflow.Comment: 14 pages, 4 figures, accepted ApJLet

Effectiveness and Efficiency of Drug Eluting Stents

Coronary artery disease (CAD), also known as ischemic heart disease (IHD) and coronary heart disease (CHD), is caused by the narrowing (stenosis) of one or more coronary arteries, due to atherosclerosis, restricting blood flow and reducing the supply of oxygen to the heart muscle. Transient shortages in blood flow and oxygen lead to angina pectoris and chest pain, which may radiate to the left shoulder, arms, neck, back or jaw. Stable angina symptoms do not tend to progress in intensity over time. More seriously, the rupturing of an atherosclerotic plaque (causing a thrombotic occlusion) and stenosis of the vessel can result in acute myocardial infarction (AMI) due to a critical reduction in the blood supply to the heart muscle (myocardial ischemia). High levels of morbidity and mortality associated with this infarction are a consequence of ischemia. It is vital to promptly re-establish coronary blood flow after an infarction, because sustained ischemic damages and injuries to the heart muscle may lead to sudden death or heart failure. In addition to infarction, acute symptomatic manifestations of ischemic heart disease include unstable angina, and less common conditions such as cardiogenic shock and sudden death (Thygesen, 2007)..