Obtención y análisis de los espectros de luminiscencia de iones de tierras raras en solución sólida

Tesis Univ. Complutense de Madrid, 1982.Depto. de ÓpticaFac. de Ciencias FísicasTRUEProQuestpu

Estado situacional del clima laboral del área académica de la Pre Universitaria “La de Lima, Grupo de Estudios y Asesoría en Matemáticas” en la ciudad de Cajamarca - 2016

RESUMEN En la actualidad, el clima laboral es una de las partes más importantes de las organizaciones tanto a nivel internacional como nacional, este terminó se viene utilizando y ejecutando desde hace muchos años atrás para lograr siempre el mejoramiento continuo, y es de mucho éxito en las empresas que aplican el bienestar del recurso humano para garantizar el éxito de la organización. En este trabajo de tesis, se va a analizar, determinar y a comparar los resultados del análisis de las tres dimensiones de estudio: Métodos de convivencia, liderazgo y trabajo en equipo, del problema que viene aquejando a la Academia Pre Universitaria La de Lima, Grupo de Estudios y Asesoría en Matemáticas en la Ciudad de Cajamarca sobre su deficiente clima laboral, el cual tiene su origen en el área académica de dicha institución. En base al problema que hemos analizado en la academia: “cual es el estado situacional del clima laboral del área Académica de la Pre Universitaria La de Lima, Grupo de Estudios y Asesoría en Matemáticas de la Ciudad de Cajamarca en el año 2016”, vamos a utilizar métodos y herramientas de coaching para solucionar este inconveniente que perjudica el ambiente de trabajo y también de alguna manera al estudiante, lo cual es parte importante de dicha institución. El objetivo primordial de este trabajo es “determinar el estado situacional del clima laboral del área Académica de la Pre Universitaria La de Lima, Grupo de Estudios y Asesoría en Matemáticas de la ciudad de Cajamarca en el año 2016” y en este nos vamos a apoyar para plantear estrategias de solución y métodos y/o herramientas con las cuales se pueda contrarrestar dicho problema. Al hablar de la hipótesis del trabajo, se muestra claramente que en las dimensiones de métodos de convivencia y trabajo en equipo existen deficiencias las cuales afectan la integridad del clima laboral, en cambio en liderazgo si se tiene una buena perspectiva. Se ha realizado una encuesta en donde se refleja lo dicho en el párrafo anterior y en base a estos resultados se puede comentar que en cuanto a la situación actual de la Academia Pre Universitaria La de Lima, Grupo de Estudios y Asesoría en Matemáticas en le Ciudad de Cajamarca, el clima laboral definitivamente no es bueno y que se debe de implementar las 11 herramientas de coaching para solucionar cuanto antes este problema y no dejar que se alargue demasiado porque esto puede ser demasiado perjudicial para el cumplimiento de metas y objetivos personales, profesionales y organizacionales. Los cuadros reflejan lo problemas y luego se hace una comparación para poder ver en qué momento se rompe el clima laboral y así de esta manera buscar la mejor solución inmediata, luego del análisis, determinación, propuesta y aplicación, esto último no se va a poder realizar por falta de tiempo, pero se va a demostrar luego de la aplicación del proceso de coaching con el post test y la encuesta, los resultados de mejora de dicho problema, dejando la institución investigada con un ambiente de compañerismo y concordia siendo este momento, el después de todo el proceso.ABSTRACT At present, the working environment is one of the most important parts of organizations both internationally and nationally, this term has been used and running since many years ago to always achieve continuous improvement, and is very successful in companies that apply the wellbeing of human resources to ensure the success of the organization. In this thesis, it is to analyze, determine and compare the results of analysis of the three dimensions of study: Methods of coexistence, leadership and teamwork, the problem that is afflicting the pre university academy the lime, group studies and consultancy in mathematics in the city of Cajamarca on their poor working environment, which has its origin in the academic area of the institution. Based on the problem we have analyzed in the academy, "which is the situational state of the labor climate in the academic area of the university pre La Lima, Group Research and Advisory Mathematics, of the city of Cajamarca in 2016" we will use coaching methods and tools to solve this problem that harms the working environment and somehow the student, which is an important part of that institution. The primary objective of this work is to "determine the situational state of the labor climate in the academic area of the university pre La Lima, Group Research and Advisory Mathematics of the city of Cajamarca in 2016" and in this we will support to raise solving strategies and methods and / or tools with which you can counter this problem. Speaking of the working hypothesis, it is clearly shown that there are deficiencies which affect the integrity of the work environment, leadership instead if you have a good perspective on the dimensions of methods of coexistence and teamwork. It has conducted a survey in which it said is reflected in the preceding paragraph and based on these results we can mention that regarding the current situation of the academy, the working environment is definitely not good and should be implemented 11 coaching tools to solve this problem as soon as possible and not let too much extra time because this may be too detrimental to the achievement of goals and personal, professional and organizational goals. The tables reflect the problems and then a comparison to see when the work environment is broken and so in this way find the best immediate solution, after analysis, determination, proposal and application is made, the latter will not be able perform for lack of time, but after the application of the coaching process is to demonstrate the post-test and survey results to improve this problem, leaving the research institution with an atmosphere of friendship and harmony and this time, after all the process

Estructura y dinámica de ribonucleasa A por espectroscopia de RMN bidimensional

Depto. de Química FísicaFac. de Ciencias QuímicasTRUEpu

The C Terminus of the Ribosomal-Associated Protein LrtA Is an Intrinsically Disordered Oligomer

The 191-residue-long LrtA protein of Synechocystis sp. PCC 6803 is involved in post-stress survival and in stabilizing 70S ribosomal particles. It belongs to the hibernating promoting factor (HPF) family, intervening in protein synthesis. The protein consists of two domains: The N-terminal region (N-LrtA, residues 1-101), which is common to all the members of the HPF, and seems to be well-folded; and the C-terminal region (C-LrtA, residues 102-191), which is hypothesized to be disordered. In this work, we studied the conformational preferences of isolated C-LrtA in solution. The protein was disordered, as shown by computational modelling, 1D-H-1 NMR, steady-state far-UV circular dichroism (CD) and chemical and thermal denaturations followed by fluorescence and far-UV CD. Moreover, at physiological conditions, as indicated by several biochemical and hydrodynamic techniques, isolated C-LrtA intervened in a self-association equilibrium, involving several oligomerization reactions. Thus, C-LrtA was an oligomeric disordered protein.This research was funded by Spanish Ministry of Economy and Competitiveness [CTQ2015-64445-R (to J.L.N.) and MAT2015-63704-P (to A.A.), with Fondo Social Europeo (ESF)], and by the Basque Government [IT-654-13 (to A.A.)

The C Terminus of the Ribosomal-Associated Protein LrtA Is an Intrinsically Disordered Oligomer

The 191-residue-long LrtA protein of Synechocystis sp. PCC 6803 is involved in post-stress survival and in stabilizing 70S ribosomal particles. It belongs to the hibernating promoting factor (HPF) family, intervening in protein synthesis. The protein consists of two domains: The N-terminal region (N-LrtA, residues 1-101), which is common to all the members of the HPF, and seems to be well-folded; and the C-terminal region (C-LrtA, residues 102-191), which is hypothesized to be disordered. In this work, we studied the conformational preferences of isolated C-LrtA in solution. The protein was disordered, as shown by computational modelling, 1D-H-1 NMR, steady-state far-UV circular dichroism (CD) and chemical and thermal denaturations followed by fluorescence and far-UV CD. Moreover, at physiological conditions, as indicated by several biochemical and hydrodynamic techniques, isolated C-LrtA intervened in a self-association equilibrium, involving several oligomerization reactions. Thus, C-LrtA was an oligomeric disordered protein.This research was funded by Spanish Ministry of Economy and Competitiveness [CTQ2015-64445-R (to J.L.N.) and MAT2015-63704-P (to A.A.), with Fondo Social Europeo (ESF)], and by the Basque Government [IT-654-13 (to A.A.)

Pancreatic Cancer: Molecular, Biochemical, Chemopreventive, and Therapeutic Aspects

Pancreatic cancer (PC) is the fourth leading cause of cancer death, with a median survival of 6 months and a dismal 5-year survival rate of 35%, a figure which has remained relatively unchanged over the past 25 years. PC is one of the most difficult diseases to treat due to late initial diagnosis and to resistance to the usual treatments. The presence of occult or clinical metastases at the time of diagnosis, together with the lack of effective chemotherapies, contributes to the high mortality in patients with PC. Its lethal nature stems from its propensity to disseminate rapidly to the lymphatic system and distant organs. Yet, understanding and stopping metastasis may prove to be one of the great potential strategies of treating PC. There is a dire need for the design of new and targeted therapeutic strategies that can overcome the drug resistance and improve the clinical outcome for patients diagnosed with the illness. The knowledge of the molecular aspects of PC is very important, and it is likely to be helpful in the design of newer drugs and the molecular selection of existing agents for targeted therapy. The inhibition of signal pathways can be carried out not only by small molecules, able to bind to selected regions of the target protein, but also by using large molecules as antibodies. The pathway to successful new therapies has been inhibited because of the rapidity with which agents tend to move into randomized, controlled trials without the extensive early testing necessary to optimize treatment regimens. However, lessons have been learned and our collective research effort has generated a substantial platform of knowledge from which further work will spring. The bioavailability of compounds such as antisense oligonucleotides and siRNAs in humans remains a big hurdle, which will require further improvement of gene-delivery strategies. Finally, the long-term goal of the therapy individualization for patients is possible if factors that predict treatment response, such as biological markers, could be determined accurately. These approaches are likely to comprise a mixture of targeted agents in combination with conventional chemotherapy and radiotherapy. For a clinically significant effect to be achieved, treatment strategies should either be in the form of (1) a horizontal approach, in which several oncogenic pathways (as those described in this series of reviews) are inhibited; or (2) a vertical approach, whereby multiple levels of a major pathway are targeted. Combination therapies, together with improved diagnostic tools and predictive markers, are ultimately desired in order to improve the bleak outlook for patients diagnosed with PC

Phosphorylation of Thr9 Affects the Folding Landscape of the N-Terminal Segment of Human AGT Enhancing Protein Aggregation of Disease-Causing Mutants

The mutations G170R and I244T are the most common disease cause in primary hyperoxaluria type I (PH1). These mutations cause the misfolding of the AGT protein in the minor allele AGT-LM that contains the P11L polymorphism, which may affect the folding of the N-terminal segment (NTT-AGT). The NTT-AGT is phosphorylated at T9, although the role of this event in PH1 is unknown. In this work, phosphorylation of T9 was mimicked by introducing the T9E mutation in the NTT-AGT peptide and the full-length protein. The NTT-AGT conformational landscape was studied by circular dichroism, NMR, and statistical mechanical methods. Functional and stability effects on the full-length AGT protein were characterized by spectroscopic methods. The T9E and P11L mutations together reshaped the conformational landscape of the isolated NTT-AGT peptide by stabilizing ordered conformations. In the context of the full-length AGT protein, the T9E mutation had no effect on the overall AGT function or conformation, but enhanced aggregation of the minor allele (LM) protein and synergized with the mutations G170R and I244T. Our findings indicate that phosphorylation of T9 may affect the conformation of the NTT-AGT and synergize with PH1-causing mutations to promote aggregation in a genotype-specific manner. Phosphorylation should be considered a novel regulatory mechanism in PH1 pathogenesis.Comunidad Valenciana CIAICO/2021/135 AULA FUNCANIS-UGRERDF/Spanish Ministry of Science, Innovation, and Universities-State Research Agency RTI2018-096246-B-I00Junta de Andalucia P18-RT-2413 ERDF/ Counseling of Economic transformation, Industry, Knowledge, and Universities B-BIO-84-UGR2

Influence of Small Quantities of Water on the Physical Properties of Alkylammonium Nitrate Ionic Liquids

This paper presents a comprehensive study of two alkylammonium nitrate ionic liquids. As part of this family of materials, mainly ethylammonium nitrate (EAN) and also propylammonium nitrate (PAN) have attracted a great deal of attention during the last decades due to their potential applications in many fields. Although there have been numerous publications focused on the measurement of their physical properties, a great dispersion can be observed in the results obtained for the same magnitude. One of the critical points to be taken into account in their physical characterization is their water content. Thus, the main objective of this work was to determine the degree of influence of the presence of small quantities of water in EAN and PAN on the measurement of density, viscosity, electrical conductivity, refractive index and surface tension. For this purpose, the first three properties were determined in samples of EAN and PAN with water contents below 30,000 ppm in a wide range of temperatures, between 5 and 95 °C, while the last two were obtained at 25 °C. As a result of this study, it has been concluded that the presence of water is critical in those physical properties that involve mass or charge transport processes, resulting in the finding that the absolute value of the average percentage change in both viscosity and electrical conductivity is above 40%. Meanwhile, refractive index (≤0.3%), density (≤0.5%) and surface tension (≤2%) present much less significant changesThis research was funded by the Spanish MINISTRY OF ECONOMY AND COMPETITIVENESS, grant numbers MAT2017-89239-C2-1-P and MAT2017-89239-C2-2-P. D. Ausín thanks the funding support of Fundación Segundo Gil Dávila. J.J. Parajó thanks the funding support of I2C postdoctoral program of Xunta de GaliciaS

Valorización de la Sociedad Minera Cerro Verde (al primer trimestre del 2016)

El presente estudio pretende realizar la valorización de la Sociedad Minera Cerro Verde (SMCV), empresa dedicada a la extracción, producción y comercialización de minerales: cobre, molibdeno y plata. Forma parte del grupo Freeport-McMoRan (Freeport) con sede principal en Arizona, Estados Unidos. Para realizar la valorización se emplearon dos metodologías: Flujo de Caja Descontado (FCD) y Múltiplos. La metodología del Modelo de Dividendos Descontados (MDD) no fue empleada debido a que SMCV mantiene como política no repartir dividendos. SMCV es el tercer productor de cobre del Perú con el 15,05% de la producción nacional en 2015, luego de la Compañía Minera Antamina (24,22%) y Southern Perú Copper (18,92%). Produce principalmente cobre (concentrado y en cátodos), alcanzando ventas por US$1.119,4 millones en 2015. A principios de 2016 fue culminado el proyecto de expansión de la planta ubicada en Arequipa, lo que demandó una inversión de US$ 4.600 millones. Luego de ello, la capacidad instalada de la planta concentradora se elevó de 120.000 a 360.000 TM/día. Esta inversión reduciría además los costos de producción debido a mejoras en la eficiencia del proceso productivo. Para realizar la valorización, se tomaron en cuenta los estados financieros al primer trimestre de 2016, la información histórica de los periodos previos y las memorias anuales. Una fuente importante de información fueron los documentos emitidos por Freeport, tanto a la Security Exchange Comission (SEC), como por los informes dirigidos a los inversionistas publicados en su página web, donde se brinda información sobre las perspectivas empresariales de la empresa y los detalles de las inversiones recientes. Para la información financiera de SMCV y de las empresas comparables se hizo uso de las herramientas financieras de la plataforma Bloomberg y de la información disponible en la Superintendencia de Mercados y Valores (SMV). Los principales factores tomados en cuenta para realizar la valorización por FCD fueron la estructura de costos luego de culminada la ampliación de la planta, el precio del cobre, el CAPEX proyectado, las obligaciones financieras futuras y la perspectiva de la economía mundial en el mediano y largo plazo

The CBS domain protein MJ0729 of Methanocaldococcus jannaschii binds DNA

AbstractThe cystathionine beta-synthase (CBS) domains function as regulatory motifs in several proteins. Elucidating how CBS domains exactly work is relevant because several genetic human diseases have been associated with mutations in those motifs. Here, we show, for the first time, that a CBS domain binds calf-thymus DNA and E-boxes recognized by transcription factors. We have carried out the DNA-binding characterization of the CBS domain protein MJ0729 from Methanocaldococcus jannaschii by biochemical and spectroscopic techniques. Binding induces conformational changes in the protein, and involves the sole tryptophan residue. The apparent dissociation constant for the E-boxes is ∼10μM. These results suggest that CBS domains might interact with DNA