Vacinação - Febre amarela

Esta webaula aborda as principais informações sobre a vacinação contra a febre amarela

Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGF.

Ovarian tissue transplantation methods using cooled and cryopreserved samples have been attractive options for fertility preservation in animal models and humans. The aim of this study was to evaluate the impact of previous exposure to cooling, cryopreservation, and VEGF on the overall efficiency of equine ovarian tissue after heterotopic xenotransplantation in mice. The end points evaluated were follicular morphology and development, follicular and stromal cell densities, angiogenesis (i.e. the density of new and mature blood vessels), collagen types I and III fiber densities, and total fibrosis. Ovaries of adult mares were harvested after ovariectomy, and ovarian fragments were xenografted in the i.p. wall of BALB nude mice. Ten types of treatments involving different combinations of cooling, cryopreservation, xenografting procedures, and VEGF exposure were compared. The novel aspect of this study was the use of equine ovarian tissue xenotransplantation in mice, challenging the fragments with different combinations of treatments. The main findings were (i) cooling but not cryopreservation was effective in preserving the follicular morphology, (ii) a greater percentage of developing follicles but lower follicular and stromal cell densities were observed after ovarian tissue engraftment, (iii) exposure to VEGF increased new and mature vessels in cryopreserved-transplanted tissue, and (iv) an appropriate balance in the collagen types I and III fiber ratio in cooling-transplanted tissue was observed after exposure to VEGF. This study contributes to advancing knowledge in the preservation of ovarian tissue after cooling-cryopreservation and transplantation aiming to be applied to genetically superior/valuable horses, livestock, endangered animals, and, possibly, humans. LAY SUMMARY: Due to ethical limitations involving humans, the female horse (mare) has recently emerged as an alternative model for reproductive comparisons with women to optimize fertility restoration using ovarian tissue transplantation techniques. This study determined if ovarian tissue from donor mares (n = 3), exposed or not to vascular endothelial growth factor (VEGF) before transplantation, better survives for 7 days after transplantation into mouse hosts (n = 12). Tissues submitted to different combinations of cooling, freezing, and transplanting treatments, along with control groups, were evaluated using the parameters morphology, development, the density of immature eggs (follicles), the density of supportive (stromal) cells, collagen protein proportions, and density of blood vessels. Frozen-thawed treatments had lower percentages of normal follicles. Exposure to VEGF increased blood vessel densities in frozen tissue and favored adequate collagen levels in cooled-transplanted treatments. In conclusion, VEGF exposure seems to be beneficial for mare ovarian tissue transplantation and warrants further investigation

A ética do silêncio racial no contexto urbano: políticas públicas e desigualdade social no Recife, 1900-1940

Mais de meio século após o preconceito racial ter se tornado o principal alvo dos movimentos urbanos pelos direitos civis nos Estados Unidos e na África do Sul, e décadas depois do surgimento dos movimentos negros contemporâneos no Brasil, o conjunto de ferramentas legislativas criado no Brasil para promover o direito à cidade ainda adere à longa tradição brasileira de silêncio acerca da questão racial. Este artigo propõe iniciar uma exploração das raízes históricas desse fenômeno, remontando ao surgimento do silêncio sobre a questão racial na política urbana do Recife, Brasil, durante a primeira metade do século XX. O Recife foi eé um exemplo paradigmático do processo pelo qual uma cidade amplamente marcada por traços negros e africanos chegou a ser definida política e legalmente como um espaço pobre, subdesenvolvido e racialmente neutro, onde as desigualdades sociais originaram na exclusão capitalista, e não na escravidão e nas ideologias do racismo científico. Neste sentido, Recife lança luzes sobre a política urbana que se gerou sob a sombra do silêncio racial.More than half a century after racial prejudice became central to urban civil rights movements in the United States and South Africa, and decades after the emergence of Brazil’s contemporary Black movements, Brazil's internationally recognized body of rights-to-the-city legislation still adheres to the country's long historical tradition of racial silence. This article explores the historical roots of this phenomenon by focusing on the emergence of racial silence in Recife, Brazil during the first half of the 20th Century. Recife was and remains a paradigmatic example of the process through which a city marked by its Black and African roots came to be legally and politically defined as a poor, underdeveloped and racially neutral space, where social inequalities derived from capitalist exclusion rather than from slavery and scientific racism. As such, Recife'sexperience sheds light on the urban policies that were generated in the shadow of racial silence

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Anorectal melanoma - histopathological and immunohistochemical features and treatment

Anorectal melanomas should be characterized by location (anal, rectal and anorectal), color, size, shape and mobility and microscopically, by melanocyte subtypes, grade of melanin pigmentation, junctional changes in the squamous epithelium, atypical mitotic index, cellular atypia, inflammatory infiltrate, vascular and perineural invasion, sentinel lymph node, and anorectal parietal penetration. Anorectal melanomas must be staged by American Joint Committee on Cancer (AJCC) and/or TNM Classification of Malignant Tumours (TNM) criteria. As melanocytes can present with several shapes, sometimes the differential diagnosis with other tumors in this region may be difficult. Because of this, immunohistochemistry is mandatory to attain a precise diagnosis. This study is a report of 14 patients with anorectal melanoma, in whom histological examinations were remade and immunohistochemistry was performed with several markers for melanocytes and for other tumor cells of the anorectal region, properly establishing the diagnosis. The most rational surgery is the extended local resection, when the disease is restricted to the area or the abdominoperineal resection to advanced lesions. Regardless of the technique used, the results are always poor. The authors deny any efficacy of current radio and/or chemotherapy as part of treatment of anorectal melanoma. Target-therapy for metastatic disease has been considered a good strategy, but the results are still inconclusive

Efeitos da prática de exercício físico sobre o desempenho da marcha e da mobilidade funcional em idosos

Introdução: A população idosa apresenta-se em um acelerado processo de crescimento associado ao aumento da expectativa de vida. As alterações no envelhecimento podem comprometer o padrão de marcha e equilíbrio dos idosos, predispondo-os a quedas. Como estratégia de prevenção, indica-se a atividade física regular direcionada para ganho de força, equilíbrio, propriocepção e melhoria da marcha. Objetivo: Verificar os efeitos de um programa de exercícios físicos na marcha e na mobilidade funcional de idosos. Materiais e métodos: Participaram do estudo oito idosos com mais de 60 anos (três homens e cinco mulheres). Os participantes realizaram duas avaliações antes e após a intervenção de seis meses: (1) marcha, pelo método de marcação de passarelas e (2) equilíbrio, por meio do Time Up and Go Test (TUGT). Para análise dos dados utilizou-se o software R (2.9.0). Para comparação dos dados, foi utilizado o teste t de Student pareado e o teste de correlação de Pearson, com p elt; 0,05. Resultados: Verificou-se aumento no comprimento dos passos E (de 0,71 ± 0,19 para 0,80 ± 0,19 cm) e D (de 0,73 ± 0,17 para 0,81 ± 0,17 cm), e das passadas E (de 1,44 ± 0,36 para 1,59 ± 0,32 cm). Para o TUGT, além de forte correlação entre idade e velocidade da marcha e base de suporte, observou-se diminuição no tempo de realização do teste (de 13,92 ± 3,84 para 9,46 ± 1,68 segundos). Conclusão: O programa de exercícios físicos direcionados para a prevenção de quedas melhorou o desempenho funcional de idosos e alterou positivamente as variáveis da marcha

Efeitos da prática de exercício físico sobre o desempenho da marcha e da mobilidade funcional em idosos

INTRODUÇÃO: A população idosa apresenta-se em um acelerado processo de crescimento associado ao aumento da expectativa de vida. As alterações no envelhecimento podem comprometer o padrão de marcha e equilíbrio dos idosos, predispondo-os a quedas. Como estratégia de prevenção, indica-se a atividade física regular direcionada para ganho de força, equilíbrio, propriocepção e melhoria da marcha. OBJETIVO: Verificar os efeitos de um programa de exercícios físicos na marcha e na mobilidade funcional de idosos. MATERIAIS E MÉTODOS: Participaram do estudo oito idosos com mais de 60 anos (três homens e cinco mulheres). Os participantes realizaram duas avaliações antes e após a intervenção de seis meses: (1) marcha, pelo método de marcação de passarelas e (2) equilíbrio, por meio do Time Up and Go Test (TUGT). Para análise dos dados utilizou-se o software R (2.9.0). Para comparação dos dados, foi utilizado o teste t de Student pareado e o teste de correlação de Pearson, com p < 0,05. RESULTADOS: Verificou-se aumento no comprimento dos passos E (de 0,71 ± 0,19 para 0,80 ± 0,19 cm) e D (de 0,73 ± 0,17 para 0,81 ± 0,17 cm), e das passadas E (de 1,44 ± 0,36 para 1,59 ± 0,32 cm). Para o TUGT, além de forte correlação entre idade e velocidade da marcha e base de suporte, observou-se diminuição no tempo de realização do teste (de 13,92 ± 3,84 para 9,46 ± 1,68 segundos). CONCLUSÃO: O programa de exercícios físicos direcionados para a prevenção de quedas melhorou o desempenho funcional de idosos e alterou positivamente as variáveis da marcha

Seasonal Abundance of Galling Insects (Hymenoptera) on Caryocar brasiliense

Caryocar brasiliense Camb. (Malpighiales: Caryocaraceae) trees have a wide distribution in the Cerrado, a tropical Brazilian savanna, with high diversity and endemism. This plant is protected by federal laws and is untouched in deforested areas of the Cerrado. This situation increases the damage to leaves from galling insects (Hymenoptera). We studied populations of galling insects and their natural enemies on C. brasiliense trees for 3 successive yr during each season in the Cerrado. A globoid gall-inducing Eurytoma sp. (Hymenoptera: Eury- tomidae) and its parasitoid Sycophila sp. (Hymenoptera: Eurytomidae) adults and predator Zelus armillatus (Lepeletier and Serville) (Hemiptera: Reduviidae) on the leaves were most abundant in the winter. The numbers of vein galls correlated negatively with the numbers of discoid and spherical galls, and the numbers of spherical galls correlated negatively with the numbers of discoid galls on C. brasiliense leaflets. Increased percentages of defoliation were correlated with reductions in the percentages of leaflets with total galls and leaflet area with total galls. Increased numbers of Sycophila sp. and decreased numbers of Ablerus magistretti Blanchard (Hymenoptera: Aphelinidae) were correlated with reduction in the numbers of Eurytoma sp. Numbers of Quadrastichus sp. (Hymenoptera: Eulophidae) and A. magistretti correlated negatively with the numbers of Sycophila sp. Increased numbers of Z. armillatus were correlated with reduction in the numbers of Eurytoma sp. and its galls and parasitoids. We concluded that this differential temporal distribution of galling insects and their natural enemies was influenced by plant phenology and time of colonization on C. brasiliense leaves.Los árboles de Caryocar brasiliense Camb. (Malpighiales: Caryocaraceae) tienen una amplia distribución en lo Cerrado, una sabana tropical de Brasil, con una alta diversidad y ende- mismo. Esta planta está protegida por las leyes federales y se deja en las áreas deforestadas de lo Cerrado. Esta situación aumenta el daño a las hojas de los insectos que causan agallas (Hymenoptera). Se estudiaron las poblaciones de insectos que causan agallas y sus enemigos naturales de los árboles de C. brasiliense durante 3 años sucesivos durante cada temporada en el Cerrado. Agallas globosas inducido por Eurytoma sp. y su parasitoide Sycophila sp. (Hymenoptera: Eurytomidae) - adultos - y depredador Zelus armillatus (Lepeletier and Serville) (Hemiptera: Reduviidae) en las hojas fueron más abundantes en el invierno. El número de agallas vena correlacionó negativamente con el número de agallas discoidales y esféricas, así como el número de agallas esféricas correlacionaron negativamente con el número de agallas discoidales en hojas del C. brasiliense. El aumento de porcentajes de defoliación se correlacionó con la reducción en los porcentajes de hojas con agallas totales y el área de la hoja con agallas totales. Aumento del número de Sycophila sp. y disminución del número de Ablerus Magistretti Blanchard (Hymenoptera: Aphelinidae) se correlacionó con la reducción en el número de Eurytoma sp. Números de Quadrastichus sp. (Hymenoptera: Eulophidae) y A. Magistretti se correlacionó negativamente con el número de Sycophila sp.. Aumento del número de Z. armillatus se correlacionó con la reducción en el número de Eurytoma sp. y sus agallas y parasitoides. Se argumenta que esta distribución diferencial temporal de insectos que causan agallas y sus enemigos naturales se vio influenciado por la fenología de la planta y el tiempo de la colonización en las hojas de C. brasiliense

Seasonal Abundance of Hemipterans on Caryocar brasiliense

Caryocar brasiliense Camb. (Malpighiales: Caryocaraceae) trees have a wide distribution in the cerrado. This plant is protected by federal laws and is untouched in deforested areas of the cerrado. Under these circumstances, the damage to leaves, flowers, and fruits from sucking hemipterans has increased. We studied populations of sucking insects and their predators on C. brasiliense trees in the cerrado during each season for 3 successive years. The numbers of sucking insect individuals on C. brasiliense trees were similar among the seasons of the year. However, the highest number of species and greatest diversity occurred in winter. Predators were most abundant in spring and winter, with highest diversity and number of species in winter. We observed 7 rare, 2 common, and 1 constant species of sucking insects; and 4 rare, 8 common, and 1 constant species of predators on C. brasiliense trees. The greatest numbers of various sucking insect species were observed by seasons as follows: Aconophora sp. (Membracidae) on fruits and Frequenamia sp. (Cicadellidae) on leaves in the winter; Aphis gossypii (Glover) (Aphididae) and Mahanarva sp. (Cercopidae) in the spring; and Dikrella sp. (Hemiptera: Cicadellidae) on the leaves in the summer and autumn. For predators, Crematogaster sp. (Hymenoptera: Formicidae) had the lowest abundance on the leaves in the summer, and highest abundance in the flowers in the winter and spring, while in spring it was most abundant on the fruits, and in the autumn Trybonia sp. (Thysanoptera: Phlaeothripidae) on the leaves was the most abundant. Higher number of ants Crematogaster sp. was observed in Caryocar brasiliense trees that presented large numbers of Dikrella sp.. Higher numbers of predators Trybonia sp., Chrysoperla sp. (Neuroptera: Chrysopidae), and total of predator thrips were associated with decreasing numbers of Dikrella sp., A. gossypii, and total of sucking insects respectively. The increase in the numbers of individuals and species of predators were correlated with the reduction of these same ecological parameters of the sucking insects. We argue that this differential temporal distribution of sucking insects and their predators was influenced for phenology of plant and weather.Los árboles de Caryocar brasiliense Camb. (Malpighiales: Caryocaraceae) tienen una amplia distribución en lo cerrado. Esta planta está protegida por las leyes federales y se deja en las áreas deforestadas de lo cerrado. Esta situación aumenta el daño a las hojas, flores y frutos de los insectos chupadores (Hemiptera). Se estudiaron las poblaciones de insectos chupadores y sus depredadores en árboles de C. brasiliense durante tres años consecutivos durante cada temporada en el cerrado. Número de ejemplares de insectos chupadores de árboles de C. brasiliense fue similar entre las estaciones del año. Sin embargo, había más especies y mayor diversidad en el invierno. Los depredadores fueron más abundantes en primavera e invierno, con mayor diversidad y número de especies durante el invierno. Hemos observado 7 raras, 2 comunes, y 1 especie constante de los insectos chupadores, y 4 raras, 8 comunes y 1 especie constante de los depredadores en árboles de C. brasiliense. El número de insectos chupadores Aconophora sp. (Membracidae) en frutas y Frequenamia sp. (Cicadellidae) en las hojas fue mayor en el invierno; Aphis gossypii (Glover) (Aphididae) y Mahanarva sp. (Cercopidae) en la primavera, y Dikrella sp. (Hemiptera: Cicadellidae) en verano y otoño en las hojas. Para los depredadores, Crematogaster sp. (Hymenoptera: Formicidae) tuvo la menor abundancia en las hojas en el verano, y la mayor abundancia en las flores en el invierno y la primavera, mientras estaba en los frutos en la primavera, y Trybonia sp. (Thysanoptera: Phlaeothripidae) en las hojas en otoño. Mayor número de hormigas Crematogaster sp. se observó en árboles del C. brasiliense que presentan un gran número de Dikrella sp.. Los números más altos de los depredadores Trybonia sp., Chrysoperla sp. (Neuroptera: Chrysopidae), y el total de los trips depredadores se asociaron con disminución del número de Dikrella sp, A. gossypii, y total de los insectos chupadores, respectivamente. El aumento en el número de individuos y especies de depredadores se correlaciona con la disminución de estos mismos parámetros ecológicos de los insectos chupadores. Nosotros sostenemos que esta distribución diferencial temporal de los insectos chupadores y sus depredadores fue influenciado por la fenología de la planta y el clima