Transient exposure to miR-203 enhances the differentiation capacity of established pluripotent stem cells

Artículo escrito por un elevado número de autores, solo se referencian el que aparece en primer lugar, el nombre del grupo de colaboración, si le hubiere, y los autores pertenecientes a la UAMFull differentiation potential along with self-renewal capacity is a major property of pluripotent stem cells (PSCs). However, the differentiation capacity frequently decreases during expansion of PSCs in vitro. We show here that transient exposure to a single microRNA, expressed at early stages during normal development, improves the differentiation capacity of already-established murine and human PSCs. Short exposure to miR-203 in PSCs (miPSCs) induces a transient expression of 2C markers that later results in expanded differentiation potency to multiple lineages, as well as improved efficiency in tetraploid complementation and human–mouse interspecies chimerism assays. Mechanistically, these effects are at least partially mediated by direct repression of de novo DNA methyltransferases Dnmt3a and Dnmt3b, leading to transient and reversible erasure of DNA methylation. These data support the use of transient exposure to miR-203 as a versatile method to reset the epigenetic memory in PSCs, and improve their effectiveness in regenerative medicineM.A.F. was supported by MICIU (SAF2014-60442-JIN). J.F.P. was funded by MICIU (RTI2018-093330-B/FEDER-EU), RamónAreces Foundation (CIVP19S7917), CAM (B2017/BMD-3778), and AECC (2018,PROYE18054PIRI). F.V.M was supported by the National Breast Cancer Foundation/Cure Cancer Australia Foundation Postdoctoral Training Fellow. Work in S.J.C laboratory was supported by the National Health and Medical ResearchCouncil (NHMRC1063560). M.A.B. laboratory is funded by SAF2013-45111-R from MICIU, Fundación Botín and Banco Santander, and Worldwide CancerResearch (WCR16-1177). Work in S.O. laboratory was funded by MICIU grant(SAF2013-44866-R). Work in the M.M. laboratory was supported by grantsfrom the MICIU (RTI2018-095582-B-I00, RED2018-102723-T, and SAF2017-92729-EXP), and the iLUNG Programme (B2017/BMD-3884) from the Comunidad de Madrid. The CNIO is a Severo Ochoa Centers of Excellence (MICIUaward SEV-2015-0510

A possible flyby anomaly for Juno at Jupiter

[EN] In the last decades there have been an increasing interest in improving the accuracy of spacecraft navigation and trajectory data. In the course of this plan some anomalies have been found that cannot, in principle, be explained in the context of the most accurate orbital models including all known effects from classical dynamics and general relativity. Of particular interest for its puzzling nature, and the lack of any accepted explanation for the moment, is the flyby anomaly discovered in some spacecraft flybys of the Earth over the course of twenty years. This anomaly manifest itself as the impossibility of matching the pre and post-encounter Doppler tracking and ranging data within a single orbit but, on the contrary, a difference of a few mm/s in the asymptotic velocities is required to perform the fitting. Nevertheless, no dedicated missions have been carried out to elucidate the origin of this phenomenon with the objective either of revising our understanding of gravity or to improve the accuracy of spacecraft Doppler tracking by revealing a conventional origin. With the occasion of the Juno mission arrival at Jupiter and the close flybys of this planet, that are currently been performed, we have developed an orbital model suited to the time window close to the perijove. This model shows that an anomalous acceleration of a few mm/s(2) is also present in this case. The chance for overlooked conventional or possible unconventional explanations is discussed. (C) 2018 COSPAR. Published by Elsevier Ltd. All rights reserved.Acedo Rodríguez, L.; Piqueras, P.; Moraño Fernández, JA. (2018). A possible flyby anomaly for Juno at Jupiter. Advances in Space Research. 61(10):2697-2706. https://doi.org/10.1016/j.asr.2018.02.037S26972706611

Genetic material manipulation and modification by optical trapping and Nanosurgery-A perspective

Light can be employed as a tool to alter and manipulate matter in many ways. An example has been the implementation of optical trapping, the so called optical tweezers, in which light can hold and move small objects with 3D control. Of interest for the Life Sciences and Biotechnology is the fact that biological objects in the size range from tens of nanometers to hundreds of microns can be precisely manipulated through this technology. In particular, it has been shown possible to optically trap and move genetic material (DNA and chromatin) using optical tweezers. Also, these biological entities can be severed, rearranged and reconstructed by the combined use of laser scissors and optical tweezers. In this review, the background, current state and future possibilities of optical tweezers and laser scissors to manipulate, rearrange and alter genetic material (DNA, chromatin and chromosomes) will be presented. Sources of undesirable effects by the optical procedure and measures to avoid them will be discussed. In addition, first tentative approaches at cellular-level genetic and organelle surgery, in which genetic material or DNA-carrying organelles are extracted out or introduced into cells, will be presente

Evaluation of Web Accessibility of Higher Education Institutions in Chile

The aim of this study is to assess the web accessibility concerning the websites of Chilean universities which are listed in The World University Rankings. Web accessibility is a fundamental factor in achieving a true educational inclusion. It is especially important in the light of the current trend of expanding not only the online content, but also online learning. What makes this even more essential is the Chilean legislation which under Law 20422 establishes the regulations regarding equality of opportunity and social inclusion of people with disabilities. The analysis has been conducted on the basis of the international standard set by the World Wide Web Consortium (W3C), version WCAG 2.0. Evaluation methodology called WCAG-EM created by the same entity, has been applied in the analysis. Various automatic web accessibility evaluation tools have also been used, apart from manual verifications. The study reveals that the websites of Chilean universities have hardly complied with the regulation and that there are barriers and difficulties of access for the elderly and/or people with disabilities.Educació

S194-P-FADD as a marker of aggressiveness and poor prognosis in human T-cell lymphoblastic lymphoma

T-cell lymphoblastic lymphoma is a haematological disease with an urgent need for reliable prognostic biomarkers that allow therapeutic stratification and dose adjustment. The scarcity of human samples is responsible for the delayed progress in the study and the clinical management of this disease, especially compared with T-cell acute lymphoblastic leukaemia, its leukemic counterpart. In the present work, we have determined by immunohistochemistry that S194-P-FADD protein is significantly reduced in a cohort of 22 samples from human T-cell lymphoblastic lymphoma. Notably, the extent of such reduction varies significantly among samples and has revealed determinant for the outcome of the tumour. We demonstrate that Fas-associated protein with death domain (FADD) phosphorylation status affects protein stability, subcellular localization and non-apoptotic functions, specifically cell proliferation. Phosphorylated FADD would be more stable and preferentially localized to the cell nucleus; there, it would favour cell proliferation. We show that patients with higher levels of S194-P-FADD exhibit more proliferative tumours and that they present worse clinical characteristics and a significant enrichment to an oncogenic signature. This supports that FADD phosphorylation may serve as a predictor for T-cell lymphoblastic lymphoma aggressiveness and clinical status. In summary, we propose FADD phosphorylation as a new biomarker with prognostic value in T-cell lymphoblastic lymphoma

Capacidad predictiva de la Batería ENFEN en el diagnóstico del Trastorno por Déficit de Atención e Hiperactividad

Attention deficit hyperactivity disorder (ADHD) is considered to be one of the most common disorders during children and adolescents’ neurodevelopment. Given how much the individuals’ executive functions are affected, the objective of the present study was to verify the validity of the Neuropsychological Assessment of Executive Functions battery for Children (ENFEN for its acronym in Spanish) as a predictor in the diagnosis of ADHD. The sample consisted of 175 participants from southeast Spain of both sexes aged between 6 and 12 (clinical M=8.39, SD=1.87, and control M=8.78, SD=1.91). The clinical group was composed of 89 (79.50%) males and 23 (20.50%) females, and the control group consisted of 37 (58.70%) males and 26 (41.30%) females. ENFEN is an individual applications battery that allows the executive functions to be globally evaluated using a wide range of elements. We followed a non-experimental research design for this comparative descriptive study. The results indicate that the phonological fluency, colour naming path, and interference scales are closely associated with the diagnosis of ADHD as they provide data on elements including inhibition, mental flexibility, sustained and selective attention, verbal fluency, and working memory. In general, this study supports the usefulness and validity of the ENFEN battery as a tool to clinically diagnose ADHD.El trastorno por déficit de atención en hiperactividad (TDAH) es uno de los trastornos más frecuentes en el neurodesarrollo de niños y adolescentes. Las personas que lo padecen se caracterizan por presentar dificultades en los procesos de atención sostenida, ser muy activos y tener un deficiente control de sus impulsos. Pese a su elevada prevalencia y la existencia de diversas pruebas utilizadas para su diagnóstico, se conocen pocos datos sobre la utilidad y validez diagnóstica de estas herramientas. Dada la gran afectación que estos sujetos presentan en las funciones ejecutivas, el objetivo de este estudio es comprobar la utilidad y validez de la batería de Evaluación Neuropsicológica de las Funciones Ejecutivas en Niños (ENFEN), como predictora herramienta de apoyo al diagnóstico de TDAH. La muestra de estudio estuvo compuesta por 175 participantes de ambos sexos, con edades comprendidas entre los 6 y 12 años (grupo clínico M=8.39, SD=1.87 y grupo control M=8.78, SD=1.91). El grupo clínico estaba compuesto por 89 (79.50%) hombres y 23 (20,50%) mujeres, y el grupo de control por 37 (58,70%) hombres y 26 (41,30%) mujeres. ENFEN es una batería de aplicación individual, compuesta por varias subpruebas que requieren el empleo de funciones ejecutivas para resolver los elementos que las componen. Se siguió un diseño no-experimental para llevar a cabo un estudio descriptivo comparativo. Los resultados señalaron que las escalas Fluidez Fonológica, Sendero Color e Interferencia que requieren en mayor medida inhibición, flexibilidad mental, atención sostenida y selectiva, fluidez verbal y memoria de trabajo, entre otras aptitudes cognitivas, son las que mejor se asocian al diagnóstico de TDAH. En general, este estudio apoya la utilidad y validez de la prueba ENFEN como herramienta para el diagnóstico clínico del TDAH

Study of structural-changes in YBa_2Cu_3O_(7-x) by cathodoluminescence in the scanning electron-microscope

Changes in the visible and infrared cathodoluminescence(CL)spectra of YBa2Cu3O7−x are detected during irradiation in the scanning electron microscope. Results indicate the influence of oxygen content on the appearance of a CL band at 450–600 nm. In irradiated samples an infrared band at 1600–1700 nm has been observed

‘Saluda’, a programme to prevent the drug consumption: a evaluation of a new version in Spanish adolescents

En la actualidad se han evaluado numerosas intervenciones preventivas de drogodependencias en el medio escolar. Sin embargo, en España son pocos los estudios que analizan la influencia precisa de cada uno de los componentes específicos a los programas con el fin de determinar cuáles se consideran cruciales para el cambio de conducta de consumo. El objetivo del presente estudio fue evaluar los efectos de una nueva versión del programa de prevención del consumo de drogas Saluda en la que se prescinde del componente fomento del ocio saludable y las tareas para casa. Esta versión reducida fue dirigida a una muestra de 106 escolares (44.6 % chicos) entre 14 y 17 años (M = 15.20; DT = 0.92) de un centro de Educación Secundaria. Se trata de un estudio exploratorio de tipo cuasi experimental y un diseño pre-post de un único grupo. Se realizó diferencia de proporciones mediante la prueba Z y diferencia de medias mediante la prueba T para muestras relacionadas. Los resultados muestran una reducción en el porcentaje de episodios de embriaguez, así como un efecto de mejora significativa sobre las variables protectoras del consumo. Se discuten estos hallazgos y se realizan propuestas para la mejora de futuras intervenciones.In Spain, as in the majority of countries in the world, drug use is a public health problem. Adolescence is considered to be a period at risk for the onset of drug use; thus, it becomes essential to intensify efforts and reduce the demand for drugs. For this purpose, numerous interventions have been evaluated, finding several sources of deficiency: among them, it is worth mentioning the lack of studies that analyzing the most effective components on drug prevention programs. In Spain, only Saluda program provides empirical evidence on the contribution of its components to the program. The objective of this study was to evaluate the effects of a new version of Saluda program on a sample of 106 students (44.6% boys) between 14 and 17 years (M = 15.20, SD = 0.92). A pilot study was conducted, in which the components “healthy leisure promotion” and “homework” were omitted. Concerning intragroup comparisons, analyses of variance on repeated measures and difference of proportions were performed. The results showed a reduction in alcohol consumption and cannabis as well as a significant improving effect on consumption protective variables. These findings were made in order to improve future preventive interventions

A delay-dependent dual-rate PID controller over an ethernet network

n this paper, a methodology to design controllers able to cope with different load conditions on an Ethernet network is introduced. Load conditions induce time-varying delays between measurements and control. To face these variations an interpolated, delay-dependent gain scheduling law is used. The lack of synchronization is solved by adopting an event-based control approach. The dual-rate control action computation is carried out at a remote controller, whereas control actions and measurements are taken out locally at the controlled process site. Stability is proved in terms of probabilistic linear matrix inequalities. TrueTime simulations in an Ethernet case show the benefit of the proposal, which is later validated on an experimental test-bed Ethernet environment.Manuscript received June 07, 2010; revised September 05, 2010; accepted September 15, 2010. Date of publication October 18, 2010; date of current version February 04, 2011. The authors A. Cuenca, J. Salt, and R. Piza are grateful to Grant PAID06-08 by the Universidad Politecnica de Valencia, Grant dpi2009-14744-c03-03 from the Spanish Ministry of Education, and Grant gv/2010/018 by Generalitat Valenciana. In addition, A. Cuenca is grateful to Grant dpi2008-06737-c02-01 by the Spanish Ministry of Education, and A. Sala is grateful to the financial support of the Spanish Ministry of Education Research Grant dpi2008-06731-c02-01, and Generalitat Valenciana Grant prometeo/2008/088. Paper no. TII-10-06-0127.Cuenca Lacruz, ÁM.; Salt Llobregat, JJ.; Sala Piqueras, A.; Pizá Fernández, R. (2011). A delay-dependent dual-rate PID controller over an ethernet network. IEEE Transactions on Industrial Informatics. 7(1):18-29. doi:10.1109/TII.2010.2085007S18297