Isolamento e identificação de microorganismos endofíticos de Campomanesia adamantium (Cambess) O. Berg (Myrtaceae) e seus metabólitos

Campomanesia adamantium (Cambess.) O. Berg (Myrtaceae) is a shrub used in folk medicine for stomach disorders and urinary tract infections. The aim of this study was isolate and identify endophytic microorganisms from C. adamantium leaves and their metabolites. The botanical material composed of C. adamantium leaves was collected near Bela Vista, Goiás. Four actinobacteria, 10 bacteria and 60 fungi were isolated. Three fungi were able to maintain the feasibility of experimentation in the laboratory: Cercospora zebrina, Agaricales sp, and Passarola daleae. Crude ethyl acetate extract was obtained from strains cultured in Czapek broth, and the produced metabolites were analyzed by electrospray ionization mass spectrometry (ESI MS). 36 metabolites were identified. This work represents the first isolation of endophytic fungi and their metabolites from C. adamantium leaves, opening future perspectives of researches with biotechnological interest in medicine, industry or agriculture.Campomanesia adamantium (Cambess.) O. Berg (Myrtaceae) é um arbusto usado na medicina popular para distúrbios do estômago e infecções do trato urinário.. O objetivo deste estudo foi isolar e identificar microorganismos endofíticos das folhas de  C. Adamantium e seus metabolitos. O material botânico composto por folhas de C. adamantium foi coletado próximo a Bela Vista, Goiás. Foram isoladas quatro actinobactérias, 10 bactérias e 60 fungos. Três fungos foram capazes de manter a viabilidade da experimentação em laboratório: Cercospora zebrina, Agaricales sp e Passarola daleae. O extrato acetato de etila foi obtido a partir de linhagens cultivadas em caldo Czapek, e os metabólitos produzidos foram analisados ​​por espectrometria de massa por ionização por electropulverização (ESI MS). 36 metabolitos foram identificados. Este trabalho representa o primeiro isolamento de fungos endofíticos e seus metabólitos das folhas de C. adamantium, abrindo perspectivas futuras de pesquisas com interesse biotecnológico na medicina, indústria ou agricultur

Carcass characteristics and meat quality of heifers fed marandu grass silage

O objetivo deste trabalho foi avaliar as características de carcaça e da carne de novilhas ¾ Zebu x Holandês submetidas a dieta com substituição da silagem de sorgo por silagem de capim‑marandu. Utilizou-se o delineamento experimental inteiramente casualizado. Os tratamentos consistiram da substituição da fração volumosa da dieta de 100, 70 e 30% de silagem de sorgo por, respectivamente, 100, 70 e 30% de silagem de capim‑marandu. Foi adicionado concentrado na proporção de 1,2% de peso corporal, e as dietas foram isoproteicas. A substituição da silagem de sorgo pela silagem de capim‑marandu aumentou a perda de água por cozimento até o ponto máximo de 49,98% de substituição, elevou a força de cisalhamento e a capacidade de retenção de água da carne, mas não influenciou os pesos e os rendimentos das carcaças, os percentuais de cortes, as medidas de carcaça, a área de olho de lombo, o pH das carcaças, a espessura de gordura e as características de cor de músculo e gordura. A substituição da silagem de sorgo por silagem de capim‑marandu diminui o ganho de peso de corpo vazio e reduz a maciez da carne de novilhas ¾ Zebu x Holandês, mas não afeta as características quantitativas da carcaça.The objective of this work was to evaluate the carcass characteristics and meat quality of ¾ Zebu x Holstein heifers subjected to a diet with the substitution of sorghum silage by marandu grass silage. A completely randomized experimental design was used. Treatments consisted of replacing the bulky fraction of the diet of 100, 70, and 30% of sorghum silage by, respectively, 100, 70, and 30% of marandu grass silage. Concentrate was added in the proportion of 1.2% of live weight, and diets were isoproteic. The substitution of sorghum silage by marandu grass silage increased water loss by cooking to the maximum point of 49.98% of substitution, elevated average shear force and water holding capacity of meat, but did not influence carcass weight and yield, cut percentiles, carcass measures, loin eye area, pH of carcasses, fat thickness, and characteristics of muscle and fat color. The substitution of sorghum silage by marandu grass silage decreases the gain of empty body weight and reduces the tenderness of meat from ¾ Zebu x Holstein heifers, but does not affect quantitative carcass traits

Comparative Effectiveness of COVID-19 Vaccines in Preventing Infections and Disease Progression from SARS-CoV-2 Omicron BA.5 and BA.2, Portugal

We estimated comparative primary and booster vaccine effectiveness (VE) of SARS-CoV-2 Omicron BA.5 and BA.2 lineages against infection and disease progression. During April-June 2022, we implemented a case-case and cohort study and classified lineages using whole-genome sequencing or spike gene target failure. For the case-case study, we estimated the adjusted odds ratios (aORs) of vaccination using a logistic regression. For the cohort study, we estimated VE against disease progression using a penalized logistic regression. We observed no reduced VE for primary (aOR 1.07 [95% CI 0.93-1.23]) or booster (aOR 0.96 [95% CI 0.84-1.09]) vaccination against BA.5 infection. Among BA.5 case-patients, booster VE against progression to hospitalization was lower than that among BA.2 case-patients (VE 77% [95% CI 49%-90%] vs. VE 93% [95% CI 86%-97%]). Although booster vaccination is less effective against BA.5 than against BA.2, it offers substantial protection against progression from BA.5 infection to severe disease.The acquisition of sequencing equipment and reagents used in this study by the Instituto Nacional de Saúde Doutor Ricardo Jorge was partially funded by the HERA project (grant no. 2021/PHF/23776) supported by the European Commission through the European Centre for Disease Control, and also partially funded by the GenomePT project (grant no. POCI-01-0145-FEDER-022184), supported by COMPETE 2020–Operational Programme for Competitiveness and Internationalisation, Lisboa Portugal Regional Operational Programme, Algarve Portugal Regional Operational, under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund, and by the Portuguese Science and Technology Foundation. Algarve Biomedical Center Laboratory received public funding through the Project ALG-D2-2021-06 Variants Screen in Southern Portugal– Monitoring Variants of Concern in Southern Portugal and the Portuguese Science and Technology Foundation national support through the Comprehensive Health Research Center (grant no. UIDP/04923/2020)info:eu-repo/semantics/publishedVersio

CURSOS DE APRIMORAMENTO EM CONSTRUÇÃO CIVIL E INSTALAÇÕES ELÉTRICAS PREDIAIS

UNIPAMPA ALEGRET

Candida albicans isolated from buccal cavity of children with Down's syndrome: occurrence and growth inhibition by Streptomyces sp

Comparação entre a presença de leveduras de Candida na cavidade bucal de crianças sem e com síndrome de Down mostrou-se estatisticamente significante no caso de crianças afetadas por esta cromossomopatia, tornando-as mais predispostas à candidíase bucal, provavelmente favorecida pelas alterações anátomo-fisiológicas da boca em decorrência da trissomia do cromossomo 21. Recidivas constantes de candidíase bucal em crianças portadoras desta alteração cromossômica levou a busca de prováveis alternativas terapêuticas. Visando determinar a atividade antifúngica de Streptomyces sp isolados de diferentes solos brasileiros, 5 cepas foram testadas frente a Candida albicans, oriundas da cavidade bucal de crianças com síndrome de Down. Observou-se que os isolados apresentaram uma diversidade de tamanho dos halos (9-31mm de diâmetro) de inibição de crescimento das leveduras, sugerindo uma possível utilização em terapêutica antifúngica.Comparison of the presence of Candida yeasts in the buccal cavity of children without and with Down's syndrome showed a statistically significant difference in the case of children that were affected by this chromosomopathy, rendering them more predisposed to buccal candidiasis, probably due to anatomicophysiologic alterations of the mouth resulting from trisomy of chromosome 21. Constant recurrence of buccal candidiasis in children with this chromosomal alteration lead to the search for a possible therapeutic alternative. Seeking to determine the antifungal activity of Streptomyces sp isolated from various Brazilian soils, 5 strains have been tested for Candida albicans isolated from the buccal cavity of children with Down's syndrome. It was observed that the isolate presented a diversity in the size of the halos (9-31mm in diameter) of growth inhibition of the yeasts, suggesting a possible use as a therapeutic antifungal

Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in Portugal

Pathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the highest impact on case numbers and fitted them into a global context, finding evidence that several international sublineages probably emerged or spread early in Portugal. We estimated a 62% infection reporting rate and that 1.3% of the population of men who have sex with men in Portugal were infected. We infer the critical role played by sexual networks and superspreader gatherings, such as sauna attendance, in the dissemination of mpox virus. Overall, our findings highlight genomic epidemiology as a tool for the real-time monitoring and control of mpox epidemics, and can guide future vaccine policy in a highly susceptible population.info:eu-repo/semantics/publishedVersio