Satellites as probes of dark matter and gravitational theories

The Milky Way hosts about 150 globular clusters, and at least 17 dwarf spheroidal galaxies. These satellites experience a constantly changing gravitational field on their orbits. Close encounters with the Galactic bulge and passages through the Galactic disk enhance the effect of the constantly changing tidal field. As a consequence satellite member stars can leave their host's gravitational potential. For globular clusters, internal mechanisms, such as 2-body relaxation are also resulting in a loss of stars. Hence, the globular clusters are constantly losing stars and are being dissolved. In this thesis I investigate 17 globular cluster for signs of dissolution. I.e., we are studying the two-dimensional distribution of (potential) cluster member stars on the sky using photometric data from the Sloan Digital Sky Survey. We use a color-magnitude weighted counting algorithm to count the stars around the globular clusters. We detect the known tidal tails of Pal 5 and NGC 5466. Further, we also confirm some previous finding of possible tidal features for NGC 5053 and NGC 6341. For NGC 4147, we observe for the first time complex two-dimensional features, resembling a multiple-arm morphology. For almost all clusters in our sample we observe a halo of extra tidal stars. We observe no new large scale tidal features for our sample of clusters containing stars brighter than ~22.5 mag. The lack of large scale tidal tails is compatible with theoretical predictions of the destruction timescales for the clusters in our sample. We also observe the two-dimensional distribution of stars around three dwarf spheroidal galaxies: Sextans, Leo II, and Ursa Minor. Each galaxy reveals a unique structure. The main, luminous body of Sextans is not filling the tidal radius. We observe an off-center peak of highest stellar density. For Leo II, we observe an almost symmetric structure, compatible with the theory that Leo II has never come close to the Milky Way. We detect the complex structure of Ursa Minor, with two off-center peaks. We observe no large scale structure emanating from this dwarf galaxy. We further investigate the possibility of a line-of-sight depth of Sextans and Ursa Minor. We study the thickness of the blue horizontal branch. For Sextans, we observe an increasing thickness with increasing radius, comparable with the photometric error. Only detailed modeling will be able to show the significance of this varying thickness. For Ursa Minor, the increase in horizontal branch thickness is negligible, compared to the photometric error. Hence, Ursa Minor shows no sign of a significant line-of-sight depth. The distribution of red and blue horizontal stars was investigated for Sextans. The ''red'' population is much more concentrated. The peak of the density of the two populations does not coincide. Further, we investigated one globular cluster in particular, Pal 14. This cluster is sparse and at a remote location in the Galaxy. We aim to answer the question whether Pal 14 is governed by classical or modified Newtonian dynamics. We measured the radial velocity of 17 red giant branch stars and (probable) AGB stars with UVES@VLT and the Keck I telescope. The resulting line-of-sight velocity dispersion is comparable to the theoretical predictions for the case of classical dynamics. The predicted value for modified dynamics is about twice as large as the observed value. With HST images we derived the cluster's mass function and computed its total mass. The main sequence mass function slope is flatter than the canonical value, the cluster seems to be depleted in lower mass stars. N-body simulations predict for a given mass of the cluster its line-of-sight velocity dispersion in modified dynamics. The measured mass for Pal 14 is requiring a much larger velocity dispersion in modified Newtonian dynamics than we have measured. This leads to the conclusion that if Pal 14 is on a circular orbit, modified dynamics cannot explain the low velocity dispersion and the measured mass simultaneously

A Wide-Field View of Leo II -- A Structural Analysis Using the SDSS

Using SDSS I data, we have analysed the stellar distribution of the Leo II dwarf spheroidal galaxy (distance of 233 kpc) to search for evidence of tidal deformation. The existing SDSS photometric catalogue contains gaps in regions of high stellar crowding, hence we filled the area at the centre of Leo II using the DAOPHOT algorithm applied to the SDSS images. The combined DAOPHOT-SDSS dataset contains three-filter photometry over a 4x4 square degree region centred on Leo II. By defining a mask in three-filter colour-magnitude space, we removed the majority of foreground field stars. We have measured the following Leo II structural parameters: a core radius of r_c = 2.64 +/- 0.19 arcmin (178 +/- 13 pc), a tidal radius of r_t = 9.33 +/- 0.47 arcmin (632 +/- 32 pc) and a total V-band luminosity of L_V = (7.4 +/- 2.0) times 10^5 L_sun (M_V = -9.9 +/- 0.3). Our comprehensive analysis of the Leo II structure did not reveal any significant signs of tidal distortion. The internal structure of this object contains only mild isophotal twisting. A small overdensity was discovered appoximately 4.5 tidal radii from the Leo II centre, however we conclude it is unlikely to be material tidally stripped from Leo II based on its stellar population, and is most likely a foreground overdensity of stars. Our results indicate that the influence of the Galactic graviational field on the structure of Leo II has been relatively mild. We rederived the mass-to-light ratio of this system using existing kinematic data combined with our improved structural measurements, and favour the scenario in which Leo II is strongly dominated by dark matter with (M/L)_V ~ 100 in solar units.Comment: 41 pages, 15 figures, accepted for publication in the Astronomical Journa

Drosophila melanogaster linker histone dH1 is required for transposon silencing and to preserve genome integrity

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial LicenseHistone H1 is an intrinsic component of chromatin, whose important contribution to chromatin structure is well-established in vitro. Little is known, however, about its functional roles in vivo. Here, we have addressed this question in Drosophila, a model system offering many advantages since it contains a single dH1 variant. For this purpose, RNAi was used to efficiently deplete dH1 in flies. Expression-profiling showsthatdH1depletion affects expression of a relatively small number of genes in a regional manner. Furthermore, depletion up-regulates inactive genes, preferentially those located in heterochromatin, while active euchromatic genes are down-regulated, suggesting that the contribution of dH1 to transcription regulation is mainly structural, organizing chromatin for proper gene-expression regulation. Up-regulated genes are remarkably enriched in transposons. In particular, R1/R2 retrotransposons, which specifically integrate in the rDNA locus, are strongly up-regulated. Actually, depletion increases expression of transposon-inserted rDNA copies, resulting in synthesis of aberrant rRNAs and enlarged nucleolus. Concomitantly, dH1-depleted cells accumulate extra-chromosomal rDNA, show increased γH2Av content, stop proliferation and activate apoptosis, indicating that depletion causes genome instability and affects proliferation. Finally, the contributions to maintenance of genome integrity and cell proliferation appear conserved in human hH1s, as their expression rescues proliferation of dH1-depleted cells. © The Author(s) 2012. Published by Oxford University Press.MICINN (CSD2006-49 and BFU2009-07111); CSIC (200420E583 and 201120E001); Generalitat de Catalunya (SGR2009-1023); IRB fellowship (to O.V.). This work was carried out within the framework of the ‘Centre de Referència en Biotecnologia’ of the ‘Generalitat de Catalunya’. Funding for open access charge: MICINN.Peer Reviewe

Testing fundamental physics with distant star clusters: theoretical models for pressure-supported stellar systems

We investigate the mean velocity dispersion and the velocity dispersion profile of stellar systems in MOND, using the N-body code N-MODY, which is a particle-mesh based code with a numerical MOND potential solver developed by Ciotti, Londrillo and Nipoti (2006). We have calculated mean velocity dispersions for stellar systems following Plummer density distributions with masses in the range of $10^4 M_\odot$ to $10^9 M_\odot$ and which are either isolated or immersed in an external field. Our integrations reproduce previous analytic estimates for stellar velocities in systems in the deep MOND regime ($a_i, a_e \ll a_0$), where the motion of stars is either dominated by internal accelerations ($a_i \gg a_e$) or constant external accelerations ($a_e \gg a_i$). In addition, we derive for the first time analytic formulae for the line-of-sight velocity dispersion in the intermediate regime ($a_i \sim a_e \sim a_0$). This allows for a much improved comparison of MOND with observed velocity dispersions of stellar systems. We finally derive the velocity dispersion of the globular cluster Pal 14 as one of the outer Milky Way halo globular clusters that have recently been proposed as a differentiator between Newtonian and MONDian dynamics.Comment: 11 pages, 9 figures, Accepted in MNRA

Osteogenic differentiation of adipose-derived canine mesenchymal stem cells seeded in porous calcium-phosphate scaffolds

IntroductionEngineered bone graft substitutes are a promising alternative and supplement to autologous bone grafts as treatments for bone healing impairment. Advances in human medicine extend an invitation to pursue these biomimetic strategies in animal patients, substantiated by the theory that specialized scaffolds, multipotent cells, and biological cues may be combined into a bioactive implant intended for the enhancement of tissue regeneration.MethodsThis proof-of-concept study was designed to evaluate and validate the feasibility of beta-tricalcium phosphate foam scaffolds seeded with canine mesenchymal stem cells derived from adipose tissue. Cell-inoculated samples and sham controls were cultured statically for 72 hours in complete growth medium to evaluate seeding capacity, while a subset of loaded scaffolds was further induced with osteogenic culture medium for 21 days. Produced implants were characterized and validated with a combination of immunofluorescence and reflection confocal microscopy, scanning electron microscopy, and polymerase chain reaction to confirm osteogenic differentiation in tridimensional-induced samples.ResultsAfter 72 hours of culture, all inoculated scaffolds presented widespread yet heterogeneous surface seeding, distinctively congregating stem cells around pore openings. Furthermore, at 21 days of osteogenic culture conditions, robust osteoblastic differentiation of the seeded cells was confirmed by the change of cell morphology and evident deposition of extra-cellular matrix, accompanied by mineralization and scaffold remodeling; furthermore, all induced cell-loaded implants lost specific stemness immunophenotype expression and simultaneously upregulated genomic expression of osteogenic genes Osterix and Ostecalcin.Conclusionsβ-TCP bio-ceramic foam scaffolds proved to be suitable carriers and hosts of canine adipose-derived MSCs, promoting not only surface attachment and proliferation, but also demonstrating strong in-vitro osteogenic potential. Although this research provides satisfactory in-vitro validation for the conceptualization and feasibility of a canine bio-active bone implant, further testing such as patient safety, large-scale reproducibility, and quality assessment are needed for regulatory compliance in future commercial clinical applications

Present-day Mass Function of Six Small Magellanic Cloud Intermediate-age and Old Star Clusters

We determined the present-day mass functions (PDMFs) of the five intermediate-age star clusters Lindsay 1, Kron 3, NGC339, NGC416, and Lindsay 38 and the old star cluster NGC121 in the Small Magellanic Cloud (SMC) based on observations with the Hubble Space Telescope Advanced Camera for Surveys. The global PDMFs are well matched by Salpeter-like power laws from their main-sequence turnoffs to 0.6 M with a power-law exponent α ranging from 1.51 0.11 (Lindsay 1) to 2.29 0.15 (NGC339). We derive total stellar masses of 105 M , except for Lindsay 38, whose mass is of the order of 104 M. Differences between the PDMFs most likely reflect the varying stages of dynamical evolution of the clusters. These SMC clusters do not follow the α versus concentration parameter c correlation as found for Galactic globular clusters of similar mass. This might be an age effect or due to their location in a galaxy where bulge and disk crossings do not play a role. No correlation is found between α and the cluster core and tidal radii (rc and rt , respectively), the half-light radii rh , age, central surface brightness, metallicity, and galactocentric radius rgc. All six clusters mass-segregated to different degrees. The two clusters Lindsay 1 and Kron 3 barely show signs for mass segregation, but have low-mass star deficient global PDMFs and might be the remnants of star clusters whose outer parts were stripped. A trend exists between the degree of mass segregation and the ratio age/relaxation time tr, h, which indicates the stage of dynamical evolution for a cluster. Our data thus suggest that the SMC clusters in the present sample had a range of initial densities and presumably different amounts of mass loss that led to different rates of dynamical evolution. The clusters' positions in the rh, m/rt versus r0/rh, m plane imply that all of the clusters are tidally filled. Our SMC clusters with projected distances larger than 3kpc from the SMC center should have Jacobi radii significantly larger than their observed King tidal radii. The clusters also have higher mean densities than the estimated central density of the SMC. It is possible that these clusters formed in a denser overall environment of the younger SMC, or that the cluster structures were unusually strongly influenced by encounters with giant molecular clouds

Expression Levels of an Alpha-Synuclein Transcript in Blood May Distinguish between Early Dementia with Lewy Bodies and Parkinson's Disease

Lewy body diseases (LBD) including dementia with Lewy bodies (DLB) and Parkinson disease (PD) are characterized by alpha-synuclein pathology. DLB is difficult to diagnose and peripheral biomarkers are urgently needed. Therefore, we analyzed the expression of five alpha-synuclein gene (SNCA) transcripts, SNCAtv1, SNCAtv2, SNCAtv3, SNCA126, and SNCA112, in 45 LBD and control temporal cortex samples and in the blood of 72 DLB, 59 PD, and 54 control subjects. The results revealed overexpression of SNCAtv1 and SNCA112 in DLB, and SNCAtv2 in PD temporal cortices. In DLB blood, diminution of all SNCA transcripts was observed. SNCAtv1 and SNCAtv2 were diminished in PD with disease onset before 70 years. SNCAtv3, driven by its own promoter, showed opposite expression in early DLB and PD, suggesting that its amount may be an early, DLB specific biomarker. Correlation between blood transcript levels and disease duration was positive in DLB and negative in PD, possibly reflecting differences in brain alpha-synuclein aggregation rates associated with differences in disease courses. In conclusion, SNCA transcripts showed a disease-specific increase in the brain and were diminished in blood of LBD patients. SNCAtv3 expression was decreased in early DLB and increased in early PD and could be a biomarker for early DLB diagnosis

Platelet miRNA Biosignature Discriminates between Dementia with Lewy Bodies and Alzheimer’s Disease

Dementia with Lewy bodies (DLB) is one of the most common causes of degenerative dementia, after Alzheimer's disease (AD), and presents pathological and clinical overlap with both AD and Parkinson's disease (PD). Consequently, only one in three DLB cases is diagnosed correctly. Platelets, previously related to neurodegeneration, contain microRNAs (miRNAs) whose analysis may provide disease biomarkers. Here, we profiled the whole platelet miRNA transcriptome from DLB patients and healthy controls. Differentially expressed miRNAs were further validated in three consecutive studies from 2017 to 2019 enrolling 162 individuals, including DLB, AD, and PD patients, and healthy controls. Results comprised a seven-miRNA biosignature, showing the highest diagnostic potential for the differentiation between DLB and AD. Additionally, compared to controls, two miRNAs were down-regulated in DLB, four miRNAs were up-regulated in AD, and two miRNAs were down-regulated in PD. Predictive target analysis identified three disease-specific clusters of pathways as a result of platelet-miRNA deregulation. Our cross-sectional study assesses the identification of a novel, highly specific and sensitive platelet-associated miRNA-based biosignature, which distinguishes DLB from AD

Glucocerebrosidase Mrna Is Diminished In Brain Of Lewy Body Diseases And Changes With Disease Progression In Blood

Parkinson disease (PD) and dementia with Lewy bodies (DLB) are Lewy body diseases characterized by abnormal alpha-synuclein deposits and overlapping pathological features in the brain. Several studies have shown that glucocerebrosidase (GBA) deficiency is involved in the development of LB diseases. Here, we aimed to find out if this deficiency starts at the transcriptional level, also involves alternative splicing, and if GBA expression changes in brain are also detectable in blood of patients with LB diseases. The expression of three GBA transcript variants (GBAtv1, GBAtv2 and GBAtv5) was analyzed in samples from 20 DLB, 25 PD and 17 control brains and in blood of 20 DLB, 26 PD patients and 17 unaffected individuals. Relative mRNA expression was determined by real-time PCR. Expression changes were evaluated by the Delta Delta Ct method. In brain, specific expression profiles were identified in the temporal cortex of DLB and in the caudate nucleus of PD. In blood, significant GBA mRNA diminution was found in both DLB and PD patients. Early PD and early-onset DLB patients showed lowest GBA levels which were normal in PD patients with advanced disease and DLB patients who developed disease after 70 years of age. In conclusion, disease group specific GBA expression profiles were found in mostly affected areas of LBD. In blood, GBA expression was diminished in LB diseases, especially in patients with early onset DLB and in patients with early PD. Age of disease onset exerts an opposite effect on GBA expression in DLB and PD