Supportive Resources: Mastering the Art of Making Sense

This paper questions the nature of qualitative user studies as currently applied in the context of experience-centered design. We suggest that conceiving knowledge as if it were an entity that can be captured in some form and transferred unchanged oversimplifies the situation in the case of design, and, for the purpose of opening a dialogue on the topic is problematic. We put forward an alternative perspective, that of supportive resources, which go beyond social science-based approaches, such as user studies, to focus on the forming of knowledge by designers. Supportive resources are intended to inspire, but equally they are intended to help frame, guide and support the design process in a non-prescriptive way. Designers can apply them as needed to support existing approaches. In order to better describe supportive resources and their role in design, the authors present four examples from projects currently being undertaken by the authors; storytelling, language and touch, material knowledge, and video

Differing calcification processes in cultured vascular smooth muscle cells and osteoblasts

© 2019 Published by Elsevier Inc.Arterial medial calcification (AMC) is the deposition of calcium phosphate mineral, often as hydroxyapatite, inthe medial layer of the arteries. AMC shares some similarities to skeletal mineralisation and has been associatedwith the transdifferentiation of vascular smooth muscle cells (VSMCs) towards an osteoblast-like phenotype. Thisstudy used primary mouse VSMCs and calvarial osteoblasts to directly compare the established and widely usedin vitromodels of AMC and bone formation. Significant differences were identified between osteoblasts andcalcifying VSMCs. First, osteoblasts formed large mineralised bone nodules that were associated with widespreaddeposition of an extracellular collagenous matrix. In contrast, VSMCs formed small discrete regions of calcifi-cation that were not associated with collagen deposition and did not resemble bone. Second, calcifying VSMCsdisplayed a progressive reduction in cell viability over time (≤7-fold), with a 50% increase in apoptosis,whereas osteoblast and control VSMCs viability remained unchanged. Third, osteoblasts expressed high levels ofalkaline phosphatase (TNAP) activity and TNAP inhibition reduced bone formation by to 90%. TNAP activity incalcifying VSMCs was∼100-fold lower than that of bone-forming osteoblasts and cultures treated withβ-gly-cerophosphate, a TNAP substrate, did not calcify. Furthermore, TNAP inhibition had no effect on VSMC calci-fication. Although, VSMC calcification was associated with increased mRNA expression of osteoblast-relatedgenes (e.g. Runx2, osterix, osteocalcin, osteopontin), the relative expression of these genes was up to 40-foldlower in calcifying VSMCs versus bone-forming osteoblasts. In summary, calcifying VSMCsin vitrodisplay somelimited osteoblast-like characteristics but also differ in several key respects: 1) their inability to form collagen-containing bone; 2) their lack of reliance on TNAP to promote mineral deposition; and, 3) the deleterious effectof calcification on their viability.Peer reviewedFinal Published versio

Individual and Familial Stressors Among Rural Nebraskan, Bilingual, Paraprofessional Educators

Individual (e.g., depression, learning styles) and familial (e.g., social support) factors affecting the psychosocial well-being of bilingual, rural Nebraska, paraprofessional educators were examined. Of 26 participants, 15 were first and 5 were second generation Hispanic immigrants. All were currently (n = 20) or formerly (n = 6) involved in an online, distance education, bachelor’s degree program in elementary education, with English as a second language certification. Results from data analyses are presented, as are suggestions for working with unique populations

The role and staffing of physiotherapy in critical care: a scoping review

ntroduction Physiotherapy services are provided to critical care units across the U.K. and internationally. U.K. guidance documents highlight potential physiotherapy roles and recommended staffing levels. However, this guidance is based on limited evidence and this scoping review was needed to inform workforce planning and future recommendations. Objectives The objectives of this scoping review were to: • Map the volume and nature of evidence in relation to physiotherapy in critical care. • Describe the role of physiotherapy within critical care. • Describe recommended physiotherapy staffing ratios in critical care. Methods Available literature between January 2009–December 2021 was searched utilising relevant databases. Studies focusing on the role of physiotherapy or physiotherapy staffing levels were included. Data extraction and appraisal was performed using relevant Joanna Briggs Institute proformas. Results A total of 1121 titles were screened, with 22 full text papers reviewed. Studies were commonly based in South Africa and United States of America and were survey based (n = 16, 72%). Literature available to define the role of physiotherapy in critical care was limited, which was further complicated by variation of practice across countries. Variability was observed for existing physiotherapy staffing levels ranging from 1:4 to 1:50 critical care beds. Discussion Based on our findings, there is limited evidence to define the role of physiotherapy within critical care, with widespread variation in existing staffing levels. Further research is required to define the role of physiotherapy in critical care and identify appropriate staffing levels in the U.K., including a focus on patient outcomes

Pathological human astroglia in Alzheimer's disease: opening new horizons with stem cell technology

Pathological remodeling, degeneration and reactivity of astrocytes are fundamental astrogliopathies contributing to all neurological diseases. In neurodegenerative disorders (including Alzheimer's disease [AD]) astroglia undergo complex changes that range from atrophy with loss of function to accumulation of reactive cells around disease-specific lesions (senile plaques in the case of AD). The cellular pathology of astroglia in the context of human AD remains enigmatic; mainly because of the severe limitations of animal models, which, although reproducing some pathological features of the disease, do not mimic its progression in full. Human-induced pluripotent stem cells technology creates a novel and potentially revolutionizing platform for studying fundamental mechanisms of the disease and for screening to identify new therapeutic compounds