Comparing Methods and Defining Practical Requirements for Extracting Harmonic Tidal Components from Groundwater Level Measurements

The groundwater pressure response to the ubiquitous Earth and atmospheric tides provides a largely untapped opportunity to passively characterize and quantify subsurface hydro-geomechanical properties. However, this requires reliable extraction of closely spaced harmonic components with relatively subtle amplitudes but well-known tidal periods from noisy measurements. The minimum requirements for the suitability of existing groundwater records for analysis are unknown. This work systematically tests and compares the ability of two common signal processing methods, the discrete Fourier transform (DFT) and harmonic least squares (HALS), to extract harmonic component properties. First, realistic conditions are simulated by analyzing a large number of synthetic data sets with variable sampling frequencies, record durations, sensor resolutions, noise levels and data gaps. Second, a model of two real-world data sets with different characteristics is validated. The results reveal that HALS outperforms the DFT in all aspects, including the ability to handle data gaps. While there is a clear trade-off between sampling frequency and record duration, sampling rates should not be less than six samples per day and records should not be shorter than 20 days when simultaneously extracting tidal constituents. The accuracy of detection is degraded by increasing noise levels and decreasing sensor resolution. However, a resolution of the same magnitude as the expected component amplitude is sufficient in the absence of excessive noise. The results provide a practical framework to determine the suitability of existing groundwater level records and can optimize future groundwater monitoring strategies to improve passive characterization using tidal signatures

Comparing Methods and Defining Practical Requirements for Extracting Harmonic Tidal Components from Groundwater Level Measurements

The groundwater pressure response to the ubiquitous Earth and atmospheric tides provides a largely untapped opportunity to passively characterize and quantify subsurface hydro-geomechanical properties. However, this requires reliable extraction of closely spaced harmonic components with relatively subtle amplitudes but well-known tidal periods from noisy measurements. The minimum requirements for the suitability of existing groundwater records for analysis are unknown. This work systematically tests and compares the ability of two common signal processing methods, the discrete Fourier transform (DFT) and harmonic least squares (HALS), to extract harmonic component properties. First, realistic conditions are simulated by analyzing a large number of synthetic data sets with variable sampling frequencies, record durations, sensor resolutions, noise levels and data gaps. Second, a model of two real-world data sets with different characteristics is validated. The results reveal that HALS outperforms the DFT in all aspects, including the ability to handle data gaps. While there is a clear trade-off between sampling frequency and record duration, sampling rates should not be less than six samples per day and records should not be shorter than 20 days when simultaneously extracting tidal constituents. The accuracy of detection is degraded by increasing noise levels and decreasing sensor resolution. However, a resolution of the same magnitude as the expected component amplitude is sufficient in the absence of excessive noise. The results provide a practical framework to determine the suitability of existing groundwater level records and can optimize future groundwater monitoring strategies to improve passive characterization using tidal signatures

Evidence for Proportionate Partition Between the Magnetic Field and Hot Gas in Turbulent Cassiopeia A

We present a deep X-ray observation of the young Galactic supernova remnant Cas A, acquired with the ROSAT High Resolution Imager. This high dynamic range (232 ks) image reveals low-surface-brightness X-ray structure, which appears qualitatively similar to corresponding radio features. We consider the correlation between the X-ray and radio morphologies and its physical implications. After correcting for the inhomogeneous absorption across the remnant, we performed a point by point (4" resolution) surface brightness comparison between the X-ray and radio images. We find a strong (r = 0.75) log-log correlation, implying an overall relationship of $\log(\Sigma_{_{\rm X-ray}}) \propto (2.21\pm0.05) \times \log(\Sigma_{_{\rm radio}})$. This is consistent with proportionate partition (and possibly equipartition) between the local magnetic field and the hot gas --- implying that Cas A's plasma is fully turbulent and continuously amplifying the magnetic field.Comment: 8 pages with embedded bitmapped figures, Accepted by ApJ Letters 5/1/9

Generation and analysis of context-specific genome-scale metabolic models derived from single-cell RNA-Seq data

Single-cell RNA sequencing combined with genome-scale metabolic models (GEMs) has the potential to unravel the differences in metabolism across both cell types and cell states but requires new computational methods. Here, we present a method for generating cell-type-specific genome-scale models from clusters of single-cell RNA-Seq profiles. Specifically, we developed a method to estimate the minimum number of cells required to pool to obtain stable models, a bootstrapping strategy for estimating statistical inference, and a faster version of the task-driven integrative network inference for tissues\ua0algorithm for generating context-specific GEMs. In addition, we evaluated the effect of different RNA-Seq normalization methods on model topology and differences in models generated from single-cell and bulk RNA-Seq data. We applied our methods on data from mouse cortex neurons and cells from the tumor microenvironment of lung cancer and in both cases found that almost every cell subtype had a unique metabolic profile. In addition, our approach was able to detect cancer-associated metabolic differences between cancer cells and healthy cells, showcasing its utility. We also contextualized models from 202 single-cell clusters across 19 human organs using data from Human Protein Atlas and made these available in the web portal Metabolic Atlas, thereby providing a valuable resource to the scientific community. With the ever-increasing availability of single-cell RNA-Seq datasets and continuously improved GEMs, their combination holds promise to become an important approach in the study of human metabolism

A Near-Infrared and X-ray Study of W49B: A Wind Cavity Explosion

We present near-infrared narrow-band images of the supernova remnant W49B, taken with the WIRC instrument on the Hale 200 inch telescope on Mt. Palomar. The 1.64 micron [Fe II] image reveals a barrel-shaped structure with coaxial rings, which is suggestive of bipolar wind structures surrounding massive stars. The 2.12 micron shocked molecular hydrogen image extends 1.9 pc outside of the [Fe II] emission to the southeast. We also present archival Chandra data, which show an X-ray jet-like structure along the axis of the [Fe II] barrel, and flaring at each end. Fitting single temperature X-ray emission models reveals: an enhancement of heavy elements, with particularly high abundances of hot Fe and Ni, and relatively metal-rich core and jet regions. We interpret these findings as evidence that W49B originated inside a wind-blown bubble (R ~ 5 pc) interior to a dense molecular cloud. This suggests that W49B's progenitor was a supermassive star, that could significantly shape its surrounding environment. We also suggest two interpretations for the jet morphology, abundance variations and molecular hydrogen emission: (1) the explosion may have been jet-driven and interacting with the molecular cavity (i.e. a Gamma-ray burst); or (2) the explosion could have been a traditional supernova, with the jet structure being the result of interactions between the shock and an enriched interstellar cloud.Comment: 9 pages with embedded figures Accepted by the Astrophysical Journa

Genome-scale metabolic network reconstruction of model animals as a platform for translational research

Genome-scale metabolic models (GEMs) are used extensively for analysis of mechanisms underlying human diseases and metabolic malfunctions. However, the lack of comprehensive and high-quality GEMs for model organisms restricts translational utilization of omics data accumulating from the use of various disease models. Here we present a unified platform of GEMs that covers five major model animals, including Mouse1 (Mus musculus), Rat1 (Rattus norvegicus), Zebrafish1 (Danio rerio), Fruitfly1 (Drosophila melanogaster), and Worm1 (Caenorhabditis elegans). These GEMs represent the most comprehensive coverage of the metabolic network by considering both orthology-based pathways and species-specific reactions. All GEMs can be interactively queried via the accompanying web portal Metabolic Atlas. Specifically, through integrative analysis of Mouse1 with RNA-sequencing data from brain tissues of transgenic mice we identified a coordinated up-regulation of lysosomal GM2 ganglioside and peptide degradation pathways which appears to be a signature metabolic alteration in Alzheimer’s disease (AD) mouse models with a phenotype of amyloid precursor protein overexpression. This metabolic shift was further validated with proteomics data from transgenic mice and cerebrospinal fluid samples from human patients. The elevated lysosomal enzymes thus hold potential to be used as a biomarker for early diagnosis of AD. Taken together, we foresee that this evolving open-source platform will serve as an important resource to facilitate the development of systems medicines and translational biomedical applications

Advancing Creativity Theory and Research: A Socio-cultural Manifesto

This manifesto, discussed by 20 scholars, representing diverse lines of creativity research, marks a conceptual shift within the field. Socio-cultural approaches have made substantial contributions to the concept of creativity over recent decades and today can provide a set of propositions to guide our understanding of past research and to generate new directions of inquiry and practice. These propositions are urgently needed in response to the transition from the Information Society to the Post-Information Society. Through the propositions outlined here, we aim to build common ground and invite the community of creativity researchers and practitioners to reflect up, study, and cultivate creativity as a socio-cultural phenomenon

A Possible Site of Cosmic Ray Acceleration in the Supernova Remnant IC 443

We present evidence for shock acceleration of cosmic rays to high energies (about 10 TeV) in the supernova remnant IC 443. X-ray imaging spectroscopy with ASCA reveals two regions of particularly hard emission: an unresolved source embedded in an extended emission region, and a ridge of emission coincident with the southeastern rim. Both features are located on part of the radio shell where the shock wave is interacting with molecular gas, and together they account for a majority of the emission at 7 keV. Though we would not have noticed it a priori, the unresolved feature is coincident with one resolved by the ROSAT HRI. Because this feature overlaps a unique region of flat radio spectral index (alpha < 0.24), has about equal light-crossing and synchrotron loss times, and a power law spectrum with a spectral index of 2, we conclude that the hard X-ray feature is synchrotron radiation from a site of enhanced particle acceleration. Evidence against a plerion includes a lack of observed periodicity (the pulsed fraction upper limit is 33%), the spectral similarity with the more extended hard region, the location of the source outside the 95% error circle of the nearby EGRET source, the fact that it is nestled in a bend in the molecular cloud ring with which IC 443 is interacting, and the requirement of an extremely high transverse velocity (>5,000 km/s). We conclude that the anomalous feature is most likely tracing enhanced particle acceleration by shocks that are formed as the supernova blast wave impacts the ring of molecular clouds.Comment: 10 pages with embedded figures; accepted by the Ap.J; author's web pages at http://lheawww.gsfc.nasa.gov/users/jonathan/ , http://lheawww.gsfc.nasa.gov/users/rob/Vita/petre.html , http://lheawww.gsfc.nasa.gov/users/evg/evg.htm

Long-Term Outcomes in Percutaneous Radiofrequency Ablation for Histologically Proven Colorectal Lung Metastasis

Introduction To evaluate the long-term outcome of image-guided radiofrequency ablation (RFA) when treating histologically confirmed colorectal lung metastasis in terms of overall survival (OS), progression-free survival (PFS) and local tumour control (LTC). Materials and Methods Retrospective single-centre study. Consecutive RFA treatments of histologically proven lung colorectal metastases between 01/01/2008 and 31/12/14. The primary outcome was patient survival (OS and PFS). Secondary outcomes were local tumour progression (LTP) and complications. Prognostic factors associated with OS/ PFS were determined by univariate and multivariate analyses. Results Sixty patients (39 males: 21 females; median age 69 years) and 125 colorectal lung metastases were treated. Eighty percent (n = 48) also underwent lung surgery for lung metastases. Mean metastasis size (cm) was 1.4 ± 0.6 (range 0.3–4.0). Median number of RFA sessions was 1 (1–4). During follow-up (median 45.5 months), 45 patients died (75%). The estimated OS and PFS survival rates at 1, 3, 5, 7, 9 years were 96.7%, 74.7%, 44.1%, 27.5%, 16.3% (median OS, 52 months) and 66.7%, 31.2%, 25.9%, 21.2% and 5.9% (median PFS, 19 months). The LTC rate was 90% with 6 patients developing LTP with 1-, 2-, 3- and 4-year LTP rates of 3.3%, 8.3%, 10.0% and 10.0%. Progression-free interval < 1 year (P = 0.002, HR = 0.375) and total number of pulmonary metastases (≥ 3) treated (P = 0.037, HR = 0.480) were independent negative prognostic factors. Thirty-day mortality rate was 0% with no intra-procedural deaths. Conclusion The long-term OS and PFS following RFA for the treatment of histologically confirmed colorectal lung metastases demonstrate comparable oncological durability to surgery