38 research outputs found
The pulmonary surfactant system matures upon pipping in the freshwater turtle Chelydra serpentina
© The Company of BiologistsPulmonary surfactant (PS), a mixture of phospholipids (PL), neutral lipids and surfactant proteins (SP), lowers surface tension within the lung, which increases lung compliance and improves the removal of fluid at birth. Here, we have examined the expression of thyroid transcription factor-1 (TTF-1) and the surfactant protein SP-B, and also the composition of pulmonary surfactant lipids in the developing lung of the turtle Chelydra serpentina. Lavage and lung tissue were collected from late embryonic, pipped and hatchling turtles. TTF-1, a regulator of gene expression of surfactant proteins and cell differentiation in mammals, was detected using immunohistochemistry in epithelia of the gas-exchange area and conducting airways during late development. Expression declined in hatchlings. SP-B was detected in subsets of cells within the respiratory epithelium at all stages sampled. The same cell types also stained for TTF-1. Turtle surfactant lipids matured toward the end of incubation. Maximal secretion of both total phospholipids and disaturated phospholipid (DSP) occurred at the time of pipping, coincident with the onset of breathing. The DSP/PL ratio increased after pipping, whereas cholesterol levels (Chol) increased prior to pipping. This resulted in a decrease in the Chol/PL and Chol/DSP ratios after pipping. Thus, TTF-1 and SP-B appear to be highly conserved within the vertebrates. Maturation of surfactant phospholipid content occurred with the commencement of pulmonary ventilation.Sonya D. Johnston, Christopher B. Daniels, David Cenzato, Jeffrey A. Whitsett and Sandra Orgei
The formation and fate of internal waves in the South China Sea
Author Posting. © The Author(s), 2015. This is the author's version of the work. It is posted here by permission of Nature Publishing Group for personal use, not for redistribution. The definitive version was published in Nature 521 (2015): 65-69, doi:10.1038/nature14399.Internal gravity waves, the subsurface analogue of the familiar surface gravity
waves that break on beaches, are ubiquitous in the ocean. Because of their strong vertical and horizontal currents, and the turbulent mixing caused by their
breaking, they impact a panoply of ocean processes, such as the supply of nutrients
for photosynthesis1, sediment and pollutant transport2 and acoustic transmission3;
they also pose hazards for manmade structures in the ocean4. Generated primarily
by the wind and the tides, internal waves can travel thousands of kilometres from
their sources before breaking5, posing severe challenges for their observation and
their inclusion in numerical climate models, which are sensitive to their effects6-7.
Over a decade of studies8-11 have targeted the South China Sea, where the oceans’
most powerful internal waves are generated in the Luzon Strait and steepen
dramatically as they propagate west. Confusion has persisted regarding their
generation mechanism, variability and energy budget, however, due to the lack of
in-situ data from the Luzon Strait, where extreme flow conditions make
measurements challenging. Here we employ new observations and numerical
models to (i) show that the waves begin as sinusoidal disturbances rather than
from sharp hydraulic phenomena, (ii) reveal the existence of >200-m-high
breaking internal waves in the generation region that give rise to turbulence levels
>10,000 times that in the open ocean, (iii) determine that the Kuroshio western
boundary current significantly refracts the internal wave field emanating from the
Luzon Strait, and (iv) demonstrate a factor-of-two agreement between modelled
and observed energy fluxes that enables the first observationally-supported energy
budget of the region. Together, these findings give a cradle-to-grave picture of
internal waves on a basin scale, which will support further improvements of their
representation in numerical climate predictions.Our work was supported by the U.S. Office of Naval Research and
the Taiwan National Science Council.2015-10-2
Cabbage and fermented vegetables : From death rate heterogeneity in countries to candidates for mitigation strategies of severe COVID-19
Large differences in COVID-19 death rates exist between countries and between regions of the same country. Some very low death rate countries such as Eastern Asia, Central Europe, or the Balkans have a common feature of eating large quantities of fermented foods. Although biases exist when examining ecological studies, fermented vegetables or cabbage have been associated with low death rates in European countries. SARS-CoV-2 binds to its receptor, the angiotensin-converting enzyme 2 (ACE2). As a result of SARS-CoV-2 binding, ACE2 downregulation enhances the angiotensin II receptor type 1 (AT(1)R) axis associated with oxidative stress. This leads to insulin resistance as well as lung and endothelial damage, two severe outcomes of COVID-19. The nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is the most potent antioxidant in humans and can block in particular the AT(1)R axis. Cabbage contains precursors of sulforaphane, the most active natural activator of Nrf2. Fermented vegetables contain many lactobacilli, which are also potent Nrf2 activators. Three examples are: kimchi in Korea, westernized foods, and the slum paradox. It is proposed that fermented cabbage is a proof-of-concept of dietary manipulations that may enhance Nrf2-associated antioxidant effects, helpful in mitigating COVID-19 severity.Peer reviewe
Nrf2-interacting nutrients and COVID-19 : time for research to develop adaptation strategies
There are large between- and within-country variations in COVID-19 death rates. Some very low death rate settings such as Eastern Asia, Central Europe, the Balkans and Africa have a common feature of eating large quantities of fermented foods whose intake is associated with the activation of the Nrf2 (Nuclear factor (erythroid-derived 2)-like 2) anti-oxidant transcription factor. There are many Nrf2-interacting nutrients (berberine, curcumin, epigallocatechin gallate, genistein, quercetin, resveratrol, sulforaphane) that all act similarly to reduce insulin resistance, endothelial damage, lung injury and cytokine storm. They also act on the same mechanisms (mTOR: Mammalian target of rapamycin, PPAR gamma:Peroxisome proliferator-activated receptor, NF kappa B: Nuclear factor kappa B, ERK: Extracellular signal-regulated kinases and eIF2 alpha:Elongation initiation factor 2 alpha). They may as a result be important in mitigating the severity of COVID-19, acting through the endoplasmic reticulum stress or ACE-Angiotensin-II-AT(1)R axis (AT(1)R) pathway. Many Nrf2-interacting nutrients are also interacting with TRPA1 and/or TRPV1. Interestingly, geographical areas with very low COVID-19 mortality are those with the lowest prevalence of obesity (Sub-Saharan Africa and Asia). It is tempting to propose that Nrf2-interacting foods and nutrients can re-balance insulin resistance and have a significant effect on COVID-19 severity. It is therefore possible that the intake of these foods may restore an optimal natural balance for the Nrf2 pathway and may be of interest in the mitigation of COVID-19 severity
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The formation and fate of internal waves in the South China Sea
Internal gravity waves, the subsurface analogue of the familiar
surface gravity waves that break on beaches, are ubiquitous in
the ocean. Because of their strong vertical and horizontal currents,
and the turbulent mixing caused by their breaking, they affect a
panoply of ocean processes, such as the supply of nutrients for
photosynthesis¹, sediment and pollutant transport² and acoustic
transmission³; they also pose hazards for man-made structures in
the ocean⁴. Generated primarily by the wind and the tides, internal
waves can travel thousands of kilometres from their sources before
breaking⁵, making it challenging to observe them and to include
them in numerical climate models, which are sensitive to their
effects[superscript 6,7]. For over a decade, studies[superscript 8–11] have targeted the South
China Sea, where the oceans’ most powerful known internal waves
are generated in the Luzon Strait and steepen dramatically as they
propagate west. Confusion has persisted regarding their mechanism
of generation, variability and energy budget, however,
owing to the lack of in situ data from the Luzon Strait, where
extreme flow conditions make measurements difficult. Here we
use new observations and numerical models to (1) show that the
waves begin as sinusoidal disturbances rather than arising from
sharp hydraulic phenomena, (2) reveal the existence of >200-metre-high breaking internal waves in the region of generation
that give rise to turbulence levels >10,000 times that in the open
ocean, (3) determine that the Kuroshio western boundary current
noticeably refracts the internal wave field emanating from the
Luzon Strait, and (4) demonstrate a factor-of-two agreement
between modelled and observed energy fluxes, which allows us to
produce an observationally supported energy budget of the region.
Together, these findings give a cradle-to-grave picture of internal
waves on a basin scale, which will support further improvements of
their representation in numerical climate predictions
Development of the pulmonary surfactant system in non-mammalian amniotes / Sonya D. Johnston.
"March 2001".Bibliography: leaves 193-238.vii, 238 leaves : ill. (some col.) ; 30 cm.Relates changes in the development of the pulmonary surfactant system in response to birth strategy, lung morphology and phylogeny in order to determine the extent of conservation in this process, by quantifying the total of phsospholipid, disaturated phospholipid and cholesterol in the lung washings of embryonic and hatchling chickens, oviparous bearded dragons and viviparous sleepy lizards, snapping turtles and green sea turtles throughout the final stages of incubation and gestation. Finds that the pattern of development of pulmonary surfactant lipids is consistent with that of mammals.Thesis (Ph.D.)--University of Adelaide, Dept. of Physiology, 200
Development of the pulmonary surfactant system in the green sea turtle, Chelonia mydas
Sonya D. Johnston; Christopher B. Daniels; David.T. Boot