Vocal Classification of Vocalizations of a Pair of Asian Small-Clawed Otters to Determine Stress

Asian Small-Clawed Otters (Aonyx cinerea) are a small, protected but threatened species living in freshwater. They are gregarious and live in monogamous pairs for their lifetimes, communicating via scent and acoustic vocalizations. This study utilized a hidden Markov model (HMM) to classify stress versus non-stress calls from a sibling pair under professional care. Vocalizations were expertly annotated by keepers into seven contextual categories. Four of these—aggression, separation anxiety, pain, and prefeeding—were identified as stressful contexts, and three of them—feeding, training, and play—were identified as non-stressful contexts. The vocalizations were segmented, manually categorized into broad vocal type call types, and analyzed to determine signal to noise ratios. From this information, vocalizations from the most common contextual categories were used to implement HMM-based automatic classification experiments, which included individual identification, stress vs non-stress, and individual context classification. Results indicate that both individual identity and stress vs non-stress were distinguishable, with accuracies above 90%, but that individual contexts within the stress category were not easily separable

Chewing gum modifies state-anxiety and alertness under conditions of social stress

Objectives: The finding that chewing gum can moderate state-anxiety under conditions of acute stress¹ has proved difficult to replicate.2,4 The present study examines the extent to which chewing gum can moderate state-anxiety under conditions of acute social stress. Method: In a between-participants design, 36 participants completed a task comprising a mock job interview (a variation on the Trier Social Stress Task3, which included a mental arithmetic component) whilst either chewing gum or without gum. Self-rated measures of mood and anxiety were taken at baseline, after a 10-minute presentation preparation stage, after the 10-minute presentation, and following a 5-minute recovery stage. Results: Post-presentation measures reflected increased state-anxiety and decrease self-rated calmness and contentedness. Chewing gum attenuated the rise in state-anxiety whilst increasing self-rated alertness. Chewing gum did not affect contentedness or calmness. Conclusions: The findings indicate that chewing gum can act to reduce anxiety under conditions of acute social stress: a finding consistent with Scholey et al.1 Furthermore, the data add to the growing body of literature demonstrating that chewing gum can increase alertness.1,2,4,

Urine Complement Proteins and the Risk of Kidney Disease Progression and Mortality in Type 2 Diabetes.

ObjectiveWe examined the association of urine complement proteins with progression to end-stage renal disease (ESRD) or death in people with type 2 diabetes and proteinuric diabetic kidney disease (DKD).Research design and methodsUsing targeted mass spectrometry, we quantified urinary abundance of 12 complement proteins in a predominantly Mexican American cohort with type 2 diabetes and proteinuric DKD (n = 141). The association of urine complement proteins with progression to ESRD or death was evaluated using time-to-event analyses.ResultsAt baseline, median estimated glomerular filtration rate (eGFR) was 54 mL/min/1.73 m2 and urine protein-to-creatinine ratio 2.6 g/g. Sixty-seven participants developed ESRD or died, of whom 39 progressed to ESRD over a median of 3.1 years and 40 died over a median 3.6 years. Higher urine CD59, an inhibitor of terminal complement complex formation, was associated with a lower risk of ESRD (hazard ratio [HR] [95% CI per doubling] 0.50 [0.29-0.87]) and death (HR [95% CI] 0.56 [0.34-0.93]), after adjustment for demographic and clinical covariates, including baseline eGFR and proteinuria. Higher urine complement components 4 and 8 were associated with lower risk of death (HR [95% CI] 0.57 [0.41-0.79] and 0.66 [0.44-0.97], respectively); higher urine factor H-related protein 2, a positive regulator of the alternative complement pathway, was associated with greater risk of death (HR [95% CI] 1.61 [1.05-2.48]) in fully adjusted models.ConclusionsIn a largely Mexican American cohort with type 2 diabetes and proteinuric DKD, urine abundance of several complement and complement regulatory proteins was strongly associated with progression to ESRD and death

Documentary Research and Archaeological Investigations at the Waite-Kirby-Potter Site, Westport, Massachusetts

Research on the Waite-Kirby-Potter house in Westport, Massachusetts, included mapping historical resources visible on the surface and excavating 25 test pits and units near the house foundations in the fall of 2009. Field investigations were complemented by extensive documentary research including a complete chain of title and genealogical research on the three families who have owned the property between the late 17th century and the present. The visible historical features include elements associated with the former stone ender (the standing stone end and chimney, an adjacent brick chimney, and a stone-lined cellar hole), stone walls, a 19th-century barn foundation, a family cemetery, and the standing Restcome Potter house. The excavations uncovered a clean gravel work yard in front of the stone end house and sheet trash scatters with artifacts from the mid-18th to early 20th centuries behind and west of the house, as well as the remains of post holes for an agricultural outbuilding or fence at the edge of the near-by agricultural field. A primary trash deposit from a space within the chimney complex was probably deposited c. 1860 and contained numerous reconstructable ceramic vessels and glass bottles. Several of the ceramic vessels date to the previous century and had been curated for some time before being discarded. The most significant contributions are to the architectural history of the property; the combination of archaeological and documentary research has suggested some new or more specific dates for events previously dated only by tradition. We suggest that the stone-end house, traditionally dated to 1677, may have been constructed in the early 18th century between 1707 and 1721 by Thomas or Benjamin Waite. The western addition to the house, attributed to David Kirby, was constructed during the period when David and his father Ichabod’s families both occupied the house (1763-1793). The construction of the Restcome Potter house has traditionally been attributed to Restcome in 1838, but the property’s previous owner David Kirby mentions his “new dwelling house” in his 1832 will, pushing the construction date of this house earlier. Finally, the modifications to the stone chimney took place after 1858, demonstrating the Potter family’s continued use and upkeep of the older house. Test pits around the foundations of the western addition to the stone ender uncovered stone foundations and sill supports intact immediately beneath the modern ground surface

Does adaptation to high altitude affect hypoxia-dependent structural plasticity of the placenta?

High altitude residence causes fetal growth restriction (FGR) during pregnancy in lowland mammals. Highland-adapted mammals do not experience this altitude-dependent FGR, suggesting that adaptation to altitude has produced some protective mechanisms. However, the specific mechanisms by which highland-adapted mammals preserve fetal growth at altitude remain unknown. We hypothesized that highland-adapted populations protect fetal growth through structural changes to the placenta that increase surface area for nutrient and gas exchange. We tested this hypothesis using deer mice (Peromyscus maniculatus), from populations native to low [400 m, Lincoln, NE] and high [4300 m, Mt. Evans, CO] altitudes. We predicted structural adaptation would occur via increases to the relative size of the labyrinth zone (LZ), the layer within the rodent placenta where nutrient and gas exchange occur. Placentas were collected from lowland and highland deer mice undergoing pregnancy under normoxia or hypoxia (60 kPa) to understand how hypoxia-dependent structural plasticity might interact with adaptive remodeling of the placenta (N = 5-7 per strain and treatment). Using immunohistochemistry, we quantified the size of each placenta zone. Our preliminary results show that highlanders have relatively larger placental arteries and LZs under both normoxia and hypoxia (P \u3c 0.05 in generalized linear mixed model), suggesting that blood delivery and area for exchange (as determined by the LZ size) may protect fetal growth in highlanders. Future work will pair histological characterization of placental structure with transcriptomics to guide a mechanistic understanding of how placentation constrains to fetal growth under hypoxia

Redox-Active Nanomaterials For Nanomedicine Applications

Nanomedicine utilizes the remarkable properties of nanomaterials for the diagnosis, treatment, and prevention of disease. Many of these nanomaterials have been shown to have robust antioxidative properties, potentially functioning as strong scavengers of reactive oxygen species. Conversely, several nanomaterials have also been shown to promote the generation of reactive oxygen species, which may precipitate the onset of oxidative stress, a state that is thought to contribute to the development of a variety of adverse conditions. As such, the impacts of nanomaterials on biological entities are often associated with and influenced by their specific redox properties. In this review, we overview several classes of nanomaterials that have been or projected to be used across a wide range of biomedical applications, with discussion focusing on their unique redox properties. Nanomaterials examined include iron, cerium, and titanium metal oxide nanoparticles, gold, silver, and selenium nanoparticles, and various nanoscale carbon allotropes such as graphene, carbon nanotubes, fullerenes, and their derivatives/variations. Principal topics of discussion include the chemical mechanisms by which the nanomaterials directly interact with biological entities and the biological cascades that are thus indirectly impacted. Selected case studies highlighting the redox properties of nanomaterials and how they affect biological responses are used to exemplify the biologically-relevant redox mechanisms for each of the described nanomaterials

Expression, Purification and Characterization of Soluble Red Rooster Laforin as a Fusion Protein in Escherichia Coli

BACKGROUND: The gene that encodes laforin, a dual-specificity phosphatase with a carbohydrate-binding module, is mutated in Lafora disease (LD). LD is an autosomal recessive, fatal progressive myoclonus epilepsy characterized by the intracellular buildup of insoluble, hyperphosphorylated glycogen-like particles, called Lafora bodies. Laforin dephosphorylates glycogen and other glucans in vitro, but the structural basis of its activity remains unknown. Recombinant human laforin when expressed in and purified from E. coli is largely insoluble and prone to aggregation and precipitation. Identification of a laforin ortholog that is more soluble and stable in vitro would circumvent this issue. RESULTS: In this study, we cloned multiple laforin orthologs, established a purification scheme for each, and tested their solubility and stability. Gallus gallus (Gg) laforin is more stable in vitro than human laforin, Gg-laforin is largely monomeric, and it possesses carbohydrate binding and phosphatase activity similar to human laforin. CONCLUSIONS: Gg-laforin is more soluble and stable than human laforin in vitro, and possesses similar activity as a glucan phosphatase. Therefore, it can be used to model human laforin in structure-function studies. We have established a protocol for purifying recombinant Gg-laforin in sufficient quantity for crystallographic and other biophysical analyses, in order to better understand the function of laforin and define the molecular mechanisms of Lafora disease

A New Planet Around an M Dwarf: Revealing a Correlation Between Exoplanets and Stellar Mass

We report precise Doppler measurements of GJ317 (M3.5V) that reveal the presence of a planet with a minimum mass Msini = 1.2 Mjup in an eccentric, 692.9 day orbit. GJ317 is only the third M dwarf with a Doppler-detected Jovian planet. The residuals to a single-Keplerian fit show evidence of a possible second orbital companion. The inclusion of an additional Jupiter-mass planet (P = 2700 days, Msini = 0.83 Mjup) improves the quality of fit significantly, reducing the rms from 12.5 m/s to 6.32 m/s. A false-alarm test yields a 1.1% probability that the curvature in the residuals of the single-planet fit is due to random fluctuations, lending additional credibility to the two-planet model. However, our data only marginally constrain a two-planet fit and further monitoring is necessary to fully characterize the properties of the second planet. To study the effect of stellar mass on Jovian planet occurrence we combine our samples of M stars, Solar-mass dwarfs and intermediate-mass subgiants. We find a positive correlation between stellar mass and the occurrence rate of Jovian planets within 2.5 AU; the former A-type stars in our sample are nearly 5 times more likely than the M dwarfs to harbor a giant planet. Our analysis shows that the correlation between Jovian planet occurrence and stellar mass remains even after accounting for the effects of stellar metallicity.Comment: ApJ accepted, 27 pages, 6 figures, 3 table