UPDATED GEOGRAPHIC DISTRIBUTIONS OF MICHIGAN HERPETOFAUNA:: A SYNTHESIS OF OLD AND NEW SOURCES

Recently a comprehensive overview of reptiles and amphibians in Michigan was published. Unfortunately, the distributions of the species represented were compiled before widespread accessibility to technological tools providing greater access to museum and historical records as well as citizen science efforts. To update the known ranges of Michigan herpetofauna, published literature, museum collections, and photographic vouchers submitted to an online database were examined and 339 new county and island records were added, updating the maps for 48 of Michigan’s 55 known species of reptiles and amphibians. I also present the first published list of Michigan amphibians that includes two new plethodontid salamanders, the Northern Dusky Salamander (Desmognathus fuscus) and Southern Two-lined Salamander (Eurycea cirrigera). This paper serves as an example of the wealth of information available to scientists that may have previously been unobtainable, and can be used for the distribution of herpetofauna elsewhere

Getting the message: The adaptive potential of interpersonal judgments

According to the Social Message Model, interpersonal judgments are transactions in which judges convey important social messages to the individuals they evaluate (the targets); targets can then respond to the judgments in more or less adaptive ways. We argue that judges’ opinions emerge from their current concerns, be it to promote their own well-being, or to foster group cohesion. Targets of judgments can best interpret the meaning of a judgment they receive by understanding the judge’s concerns, competence of the judge, and other qualities of the transaction. We suggest that judges and targets who are better able to reason about the judgment process are likely to change their behaviors more adaptively than people less able to reason in this area

First-principles calculation of DNA looping in tethered particle experiments

We calculate the probability of DNA loop formation mediated by regulatory proteins such as Lac repressor (LacI), using a mathematical model of DNA elasticity. Our model is adapted to calculating quantities directly observable in Tethered Particle Motion (TPM) experiments, and it accounts for all the entropic forces present in such experiments. Our model has no free parameters; it characterizes DNA elasticity using information obtained in other kinds of experiments. [...] We show how to compute both the "looping J factor" (or equivalently, the looping free energy) for various DNA construct geometries and LacI concentrations, as well as the detailed probability density function of bead excursions. We also show how to extract the same quantities from recent experimental data on tethered particle motion, and then compare to our model's predictions. [...] Our model successfully reproduces the detailed distributions of bead excursion, including their surprising three-peak structure, without any fit parameters and without invoking any alternative conformation of the LacI tetramer. Indeed, the model qualitatively reproduces the observed dependence of these distributions on tether length (e.g., phasing) and on LacI concentration (titration). However, for short DNA loops (around 95 basepairs) the experiments show more looping than is predicted by the harmonic-elasticity model, echoing other recent experimental results. Because the experiments we study are done in vitro, this anomalously high looping cannot be rationalized as resulting from the presence of DNA-bending proteins or other cellular machinery. We also show that it is unlikely to be the result of a hypothetical "open" conformation of the LacI tetramer.Comment: See the supplement at http://www.physics.upenn.edu/~pcn/Ms/TowlesEtalSuppl.pdf . This revised version accepted for publication at Physical Biolog

TURBULENCE IN MOLECULAR CLOUDS

We generate random Gaussian turbulent velocity fields with a Kolmogorov spectrum and use these to obtain synthetic line-of-sight velocity profiles. The profiles are found to be similar to line profiles observed in molecular clouds. We suggest methods for analysing measured line profiles to test whether they might arise from Gaussian Kolmogorov turbulence.Comment: accepted in ApJ, compressed postscript, figures not included. Complete preprint available at http://ucowww.ucsc.edu/~dubinski/home.html or by request to [email protected]

Concentration and Length Dependence of DNA Looping in Transcriptional Regulation

In many cases, transcriptional regulation involves the binding of transcription factors at sites on the DNA that are not immediately adjacent to the promoter of interest. This action at a distance is often mediated by the formation of DNA loops: Binding at two or more sites on the DNA results in the formation of a loop, which can bring the transcription factor into the immediate neighborhood of the relevant promoter. These processes are important in settings ranging from the historic bacterial examples (bacterial metabolism and the lytic-lysogeny decision in bacteriophage), to the modern concept of gene regulation to regulatory processes central to pattern formation during development of multicellular organisms. Though there have been a variety of insights into the combinatorial aspects of transcriptional control, the mechanism of DNA looping as an agent of combinatorial control in both prokaryotes and eukaryotes remains unclear. We use single-molecule techniques to dissect DNA looping in the lac operon. In particular, we measure the propensity for DNA looping by the Lac repressor as a function of the concentration of repressor protein and as a function of the distance between repressor binding sites. As with earlier single-molecule studies, we find (at least) two distinct looped states and demonstrate that the presence of these two states depends both upon the concentration of repressor protein and the distance between the two repressor binding sites. We find that loops form even at interoperator spacings considerably shorter than the DNA persistence length, without the intervention of any other proteins to prebend the DNA. The concentration measurements also permit us to use a simple statistical mechanical model of DNA loop formation to determine the free energy of DNA looping, or equivalently, the J-factor for looping

Randomised, controlled trial of alternating pressure mattresses compared with alternating pressure overlays for the prevention of pressure ulcers : PRESSURE (pressure relieving support surfaces) trial

Objective To compare whether differences exist between alternating pressure overlays and alternating pressure mattresses in the development of new pressure ulcers, healing of existing pressure ulcers, and patient acceptability. Design Pragmatic, open, multicentre, randomised controlled trial. Setting 11 hospitals in six NHS trusts. Participants 1972 people admitted to hospital as acute or elective patients. Interventions Participants were randomised to an alternating pressure mattress (n = 982) of- an alternating pressure overlay (n = 990). Main outcome measures The proportion of participants developing a new pressure ulcer of grade 2 or worse; time to development of new pressure ulcers; proportions of participants developing a new ulcer within 30 days; healing of existing pressure ulcers; and patient acceptability Results Intention to treat analysis found no difference in the proportions of participants developing a new pressure ulcer of grade 2 or worse (10.7% overlay patients, 10.3% mattress patients; difference 0.4%, 95% confidence interval - 23% to 3.1%, P = 0.75). More overlay patients requested change owing to dissatisfaction (23.3%) than mattress patients (18.9%, P = 0.02). Conclusion No difference was found between alternating pressure mattresses and alternating pressure overlays in the proportion of people who develop a pressure ulcer

Atomic Carbon in M82

We report observations of C I(^3P_1 - ^3P_0) emission at 492 GHz toward the starburst galaxy M82. Both the C I/C II intensity ratio and the C/CO column density ratio are a factor of 2-5 higher than observed toward Galactic photodissociation regions (PDRs) or predicted by PDR models. We argue that current PDR models are insufficient to explain the observations, and propose that some of the emission is due to atomic carbon existing within molecular clouds. Employing new chemical models, which use a fast H_3^+ dissociative recombination rate, we find that enhanced cosmic-ray flux supplied by supernova remnants in the M82 starburst lead to an enhanced atomic carbon abundance and elevated temperatures deep within the molecular clouds, resulting in a higher C I emissivity than found in previous PDR models

Plans for a 10-m Submillimeter-wave Telescope at the South Pole

A 10 meter diameter submillimeter-wave telescope has been proposed for the NSF Amundsen-Scott South Pole Station. Current evidence indicates that the South Pole is the best submillimeter-wave telescope site among all existing or proposed ground-based observatories. Proposed scientific programs place stringent requirements on the optical quality of the telescope design. In particular, reduction of the thermal background and offsets requires an off-axis, unblocked aperture, and the large field of view needed for survey observations requires shaped optics. This mix of design elements is well-suited for large scale (square degree) mapping of line and continuum radiation from submillimeter-wave sources at moderate spatial resolutions (4 to 60 arcsecond beam size) and high sensitivity (milliJansky flux density levels). the telescope will make arcminute angular scale, high frequency Cosmic Microwave Background measurements from the best possible ground-based site, using an aperture which is larger than is currently possible on orbital or airborne platforms. Effective use of this telescope will require development of large (1000 element) arrays of submillimeter detectors which are background-limited when illuminated by antenna temperatures near 50 K.Comment: 12 pages, 3 figure

Calibrating Type Ia Supernovae using the Planetary Nebula Luminosity Function I. Initial Results

We report the results of an [O III] lambda 5007 survey for planetary nebulae (PN) in five galaxies that were hosts of well-observed Type Ia supernovae: NGC 524, NGC 1316, NGC 1380, NGC 1448 and NGC 4526. The goals of this survey are to better quantify the zero-point of the maximum magnitude versus decline rate relation for supernovae Type Ia and to validate the insensitivity of Type Ia luminosity to parent stellar population using the host galaxy Hubble type as a surrogate. We detected a total of 45 planetary nebulae candidates in NGC 1316, 44 candidates in NGC 1380, and 94 candidates in NGC 4526. From these data, and the empirical planetary nebula luminosity function (PNLF), we derive distances of 17.9 +0.8/-0.9 Mpc, 16.1 +0.8/-1.1 Mpc, and 13.6 +1.3/-1.2 Mpc respectively. Our derived distance to NGC 4526 has a lower precision due to the likely presence of Virgo intracluster planetary nebulae in the foreground of this galaxy. In NGC 524 and NGC 1448 we detected no planetary nebulae candidates down to the limiting magnitudes of our observations. We present a formalism for setting realistic distance limits in these two cases, and derive robust lower limits of 20.9 Mpc and 15.8 Mpc, respectively. After combining these results with other distances from the PNLF, Cepheid, and Surface Brightness Fluctuations distance indicators, we calibrate the optical and near-infrared relations for supernovae Type Ia and we find that the Hubble constants derived from each of the three methods are broadly consistent, implying that the properties of supernovae Type Ia do not vary drastically as a function of stellar population. We determine a preliminary Hubble constant of H_0 = 77 +/- 3 (random) +/- 5 (systematic) km/s/Mpc for the PNLF, though more nearby galaxies with high-quality observations are clearly needed.Comment: 25 pages, 12 figures. Accepted for publication by the Astrophysical Journal. Figures degraded to comply with limit. Full paper is available at: http://www.as.ysu.edu/~jjfeldme/pnlf_Ia.pd