Tissue-selective controlled decorin gene delivery in the rabbit cornea significantly retards corneal angiogenesis in vivo [abstract]

Recent studies have shown that decorin gene therapy inhibits neovascularization in many non-ocular tissues. We tested the efficacy of decorin gene delivery into stroma with AAV5 to impede vascular endothelial growth factor (VEGF)-induced angiogenesis in rabbit cornea in vivo

Operationalising factors that explain the emergence of infectious diseases : A case study of the human campylobacteriosis epidemic

Peer reviewedPublisher PD

AAV5-mediated targeted decorin gene therapy : effective and safe for corneal fibrosis [abstract]

Corneal fibrosis is 3rd leading cause of global blindness according to WHO report. At present, no agents are proven to clinically reduce corneal fibrosis without causing significant side effects. It was hypothesized that decorin gene delivered into keratocytes prevents corneal fibrosis in the cornea in vivo by blocking transforming growth factor β (TGFb), which converts keratocyte to myofibroblasts and cause fibrosis

INVOLVEMENT WITHOUT PANIC: ESSAYS ON SHAKESPEARE, WOOLF, AND MUNRO

This thesis portfolio is comprised of an introductory essay and three essays of literary critique. The introductory essay is a general commentary upon reading, literature, and criticism, and introduces the three subsequent essays. The first essay is on William Shakespeare’s play, Measure for Measure. The second essay is on Virginia Woolf’s novel, The Waves. And, finally, the third essay is on the stories of Alice Munro

N/P ratio in the PEI2-GNP-DNA complex affects transgene delivery in the human cornea in vitro

Recently, we discovered that polyethylenimine-conjugated gold nanoparticles (PEI2- GNP) could be used as gene therapy vector for the cornea. It was hypothesized that DNA concentration, incubation timing and PEI monomer amount in transfection solution affect gene transfer efficiency and toxicity. The aims of this study were to test whether molar ratio of PEI2 nitrogen (N) and phosphate (P) of DNA in PEI2-GNP transfection solution regulates transgene delivery in human corneal fibroblasts in vitro, and examine PEI2-GNP toxicity, uptake and clearance for the cornea in vivo."National Eye Institute, NIH, Bethesda for RO1EY017294 (RRM), Diversity (RRM) and Veteran Health Affairs Merit (RRM) grants and Unrestricted grant from Research to Prevent Blindness, New York

SAHA : FDA approved histone deacetylase inhibitor demonstrates exceptionally high inhibition of corneal haze following PRK surgery in rabbit model [abstract]

TGF[beta] induces the transformation of corneal keratocytes into fibroblasts and myofibroblasts resulting in the formation of corneal haze (scar) following injury. We investigated whether epigenetic modifications can prevent development of corneal haze in vivo using a rabbit model

A multi-country outbreak of Salmonella newport gastroenteritis in Europe associated with watermelon from Brazil, confirmed by whole genome sequencing:October 2011 to January 2012

In November 2011, the presence of Salmonella Newport in a ready-to-eat watermelon slice was confirmed as part of a local food survey in England. In late December 2011, cases of S. Newport were reported in England, Wales, Northern Ireland, Scotland, Ireland and Germany. During the outbreak, 63 confirmed cases of S. Newport were reported across all six countries with isolates indistinguishable by pulsed-field gel electrophoresis from the watermelon isolate. A subset of outbreak isolates were whole-genome sequenced and were identical to, or one single nucleotide polymorphism different from the watermelon isolate. In total, 46 confirmed cases were interviewed of which 27 reported watermelon consumption. Further investigations confirmed the outbreak was linked to the consumption of watermelon imported from Brazil. Although numerous Salmonella outbreaks associated with melons have been reported in the United States and elsewhere, this is the first of its kind in Europe. Expansion of the melon import market from Brazil represents a potential threat for future outbreaks. Whole genome sequencing is rapidly becoming more accessible and can provide a compelling level of evidence of linkage between human cases and sources of infection, to support public health interventions in global food markets

Moses or Ishmael

https://digitalcommons.acu.edu/crs_books/1260/thumbnail.jp