Online administration system for small and medium-sized running events

The purpose of this project is to address inefficiency at small to medium-sized running events with regards to registration, race timing, and result service. As a race coordinator or participant, it is important that the race timing is correct and that the result service can be delivered as soon as possible after the event in finished. Contains source code

Mitochondrial Group I Introns in Hexacorals Are Regulatory Genetic Elements

Hexacoral mitochondrial genomes are highly economically organized and vertebrate-like in size, structure, and gene content. A hallmark, however, is the presence of group I introns interrupting essential oxidative phosphorylation (OxPhos) genes. Two genes, encoding NADH dehydrogenase subunit 5 (ND5) and cytochrome c oxidase subunit I (COI), are interrupted with introns. The ND5 intron, located at position 717, is obligatory in all hexacoral specimens investigated. The ND5-717 intron is a giant-sized intron that carries several canonical OxPhos genes. Different modes of splicing appear to apply for the ND5-717 intron, including conventional cis-splicing, backsplicing, and trans-splicing. Three distinct versions of hexacoral COI introns are noted at genic positions 884, 867, and 720. The COI introns are of the mobile-type, carrying homing endonuclease genes (HEGs). Some COI-884 intron HEGs are highly expressed as in-frame COI exon fusions, while the expression of COI-867 intron HEGs appear repressed. We discuss biological roles of hexacoral mitochondrial ND5 and COI introns and suggest that the ND5-717 intron has gained new regulatory functions beyond self-splicing

Expanding the Coding Potential of Vertebrate Mitochondrial Genomes: Lesson Learned from the Atlantic Cod

Vertebrate mitochondrial genomes are highly conserved in structure, gene content, and function. Most sequenced mitochondrial genomes represent bony fishes, and that of the Atlantic cod (Gadus morhua) is the best characterized among the fishes. In addition to the well-characterized 37 canonical gene products encoded by vertebrate mitochondrial genomes, new classes of gene products representing peptides and noncoding RNAs have been discovered. The Atlantic cod encodes at least two peptides (MOTS-c and humanin (HN)), two long noncoding RNAs (lncCR-L and lncCR-H), and a number of small RNAs. Here, we review recent research in the Atlantic cod focusing on putative mitochondrial-derived peptides, the mitochondrial transcriptome, and noncoding RNAs

Giant group I intron in a mitochondrial genome is removed by RNA back-splicing

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High-throughput identification of adapters in single-read sequencing data

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From direct attendance to telephone triage in an emergency primary healthcare service: An observational study

Objectives To describe how an intervention to limit direct attendance in an emergency primary healthcare service affected the contacts to the clinic and the level of care given, and which factors were associated with a change from direct attendance to telephone contact. Design Observational study. Setting Seven Norwegian emergency primary healthcare services. The telephone triage operators are primarily registered nurses. Participants Registered patient contacts to the services during 2007–2019. Interventions In 2013, one of the seven services made an intervention to limit direct attendances to the emergency primary healthcare clinic. Through an advertisement in a local newspaper, the public was encouraged to call in advance. Patients who still attended directly, were encouraged to call in advance next time. Measures We compared the proportions of direct attendance and telephone contact, and of consultation by a general practitioner and telephone consultation by an operator, before and after the intervention. We also compared the proportions of direct attendance regarding gender, age group, time of day and urgency level. Descriptive analyses and log binomial regression analyses were applied. Results There were 1 105 019 contacts to the seven services during the study period. The average proportion of direct attendance decreased from 68.7% (95% CI 68.4% to 68.9%) to 23.4% (95% CI 23.2% to 23.6%) in the service that carried out the intervention. Telephone consultation by an operator increased from 11.7% (95% CI 11.5% to 11.8%) to 29.2% (95% CI 28.9% to 29.5%) and medical consultation by a general practitioner decreased from 78.3% (95% CI 78.1% to 78.5%) to 57.0% (95% CI 56.7% to 57.3%). The youngest and the oldest age group and women had the largest decrease in direct attendance, by −81%, −74% and −71%, respectively. Conclusion The intervention influenced how the public contacted the service. Information campaigns on how to contact healthcare services should be implemented on a regular basis.publishedVersio

The mitochondrial transcriptome of the anglerfish Lophius piscatorius

Objective Analyze key features of the anglerfish Lophius piscatorius mitochondrial transcriptome based on high-throughput total RNA sequencing. Results We determined the complete mitochondrial DNA and corresponding transcriptome sequences of L. piscatorius. Key features include highly abundant mitochondrial ribosomal RNAs (10–100 times that of mRNAs), and that cytochrome oxidase mRNAs appeared > 5 times more abundant than both NADH dehydrogenase and ATPase mRNAs. Unusual for a vertebrate mitochondrial mRNA, the polyadenylated COI mRNA was found to harbor a 75 nucleotide 3′ untranslated region. The mitochondrial genome expressed several non-canonical genes, including the long noncoding RNAs lncCR-H, lncCR-L and lncCOI. Whereas lncCR-H and lncCR-L mapped to opposite strands in a non-overlapping organization within the control region, lncCOI appeared novel among vertebrates. We found lncCOI to be a highly abundant mitochondrial RNA in antisense to the COI mRNA. Finally, we present the coding potential of a humanin-like peptide within the large subunit ribosomal RNA.publishedVersio

The Mitochondrial Genome of the Sea Anemone Stichodactyla haddoni Reveals Catalytic Introns, Insertion-Like Element, and Unexpected Phylogeny

A hallmark of sea anemone mitochondrial genomes (mitogenomes) is the presence of complex catalytic group I introns. Here, we report the complete mitogenome and corresponding transcriptome of the carpet sea anemone Stichodactyla haddoni (family Stichodactylidae). The mitogenome is vertebrate-like in size, organization, and gene content. Two mitochondrial genes encoding NADH dehydrogenase subunit 5 (ND5) and cytochrome c oxidase subunit I (COI) are interrupted with complex group I introns, and one of the introns (ND5-717) harbors two conventional mitochondrial genes (ND1 and ND3) within its sequence. All the mitochondrial genes, including the group I introns, are expressed at the RNA level. Nonconventional and optional mitochondrial genes are present in the mitogenome of S. haddoni. One of these gene codes for a COI-884 intron homing endonuclease and is organized in-frame with the upstream COI exon. The insertion-like orfA is expressed as RNA and translocated in the mitogenome as compared with other sea anemones. Phylogenetic analyses based on complete nucleotide and derived protein sequences indicate that S. haddoni is embedded within the family Actiniidae, a finding that challenges current taxonomy