105 research outputs found

    Quantum Particle Swarm Optimization Applied to Distinct Remuneration Approaches in Demand Response Programs

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    The development of demand response programs has been allowing to improve power system performance in several ways, both in terms of the management of electricity markets, as well as regarding benefits in its operation. In order to model the remuneration for the participation of consumers in the scheduling of resources, this paper proposes a methodology based on the use of four incentive-based tariffs for the remuneration of demand response participation. It considers steps, quadratic, constant and linear remuneration. The optimization model enables Virtual Power Players to minimize operation costs, considering different critical situations of management and operation. The optimization problem has been solved by Quantum Particle Swarm Optimization. The case study concerns 168 consumers, classified into 5 consumer types, 118 distributed generation resources and 4 external suppliers.The present work was done and funded in the scope of the following projects: EUREKA - ITEA2 Project SEAS with project number 12004; ELECON Project, REA grant agreement No 318912 (FP7 PIRSES-GA2012-318912); H2020 DREAM-GO Project (Marie Sklodowska-Curie grant agreement No 641794); and UID/EEA/00760/2013 funded by FEDER Funds through COMPETE program and by National Funds through FCT.info:eu-repo/semantics/publishedVersio

    Draft genome sequence of <i>Bacillus thuringiensis</i> strain BrMgv02-JM63, a chitinolytic bacterium isolated from oil-contaminated mangrove soil in Brazil

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    Here, we report the draft genome sequence and the automatic annotation of Bacillus thuringiensis strain BrMgv02-JM63. This genome comprises a set of genes involved in the metabolism of chitin and N-acetylglucosamine utilization, thus suggesting the possible role of this strain in the cycling of organic matter in mangrove soils

    Análise do perfil epidemiológico de notificações de sífilis na cidade de Varginha-MG nos anos de 2007 a 2017/Epidemiological profile of syphilis notifications analyses in the city of Varginha-MG in the years 2007 to 2017

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    A sífilis é uma doença crônica causada por uma infecção bacteriana, tendo Treponema pallidum como agente etiológico e que pode se manifestar em vários órgãos como fígado, pele e coração. No Brasil, a sífilis é um problema de saúde pública principalmente entre as gestantes, e o maior índice de notificações se concentra nas regiões nordeste, sudeste e sul. Diante da relevância da sífilis no campo da saúde pública e da necessidade de se conhecer a realidade de cada local para melhor desenvolvimento de medidas de controle, o presente estudo realizou uma análise do perfil epidemiológico da ocorrência de sífilis na cidade de Varginha-MG nos anos de 2007 a 2017, destacando seus principais grupos de risco. Foi observado um aumento de casos da doença na população masculina, porém predominância para o grupo de gestantes. Medidas efetivas visando não apenas a diminuição de novos casos como o tratamento dos indivíduos infectados deve ser urgentemente priorizada. A intensificação das campanhas de conscientização segue como uma das melhores formas de se combater essa realidade

    Pesquisa sorológica de Ehrlichia sp. em cães da região central do Rio Grande do Sul

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    A serological survey on Ehrlichia canis was conducted among dogs in the central area of the state of Rio Grande do Sul, where the tick Rhipicephalus sanguineus is a common parasite of dogs. Out of a total of 316 dogs attended at the veterinary teaching hospital in the municipality of Santa Maria, only 14 (4.43%) reacted positively to E. canis antigens in the indirect immunofluorescence assay, with the following endpoint titers: 80 (three dogs), 160 (five), 320 (four), 640 (one) and 1280 (one). Like in previous studies in other regions of the state of Rio Grande do Sul, only a very small portion of the dogs in Santa Maria presented antibodies reactive to E. canis, even though canine infestations due to R. sanguineus are very common in this study region. These results contrast with other regions of Brazil, where E. canis is endemic among canine populations, with seropositivity values generally higher than 30%. Genetic differences among the R. sanguineus populations in South America might be implicated in these contrasting results

    Natural blood feeding and temperature shift modulate the global transcriptional profile of Rickettsia rickettsii infecting its tick vector

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    Rickettsia rickettsii is an obligate intracellular tick-borne bacterium that causes Rocky Mountain Spotted Fever (RMSF), the most lethal spotted fever rickettsiosis. When an infected starving tick begins blood feeding from a vertebrate host, R. rickettsii is exposed to a temperature elevation and to components in the blood meal. These two environmental stimuli have been previously associated with the reactivation of rickettsial virulence in ticks, but the factors responsible for this phenotype conversion have not been completely elucidated. Using customized oligonucleotide microarrays and high-throughput microfluidic qRT-PCR, we analyzed the effects of a 10 degrees C temperature elevation and of a blood meal on the transcriptional profile of R. rickettsii infecting the tick Amblyomma aureolatum. This is the first study of the transcriptome of a bacterium in the genus Rickettsia infecting a natural tick vector. Although both stimuli significantly increased bacterial load, blood feeding had a greater effect, modulating five-fold more genes than the temperature upshift. Certain components of the Type IV Secretion System (T4SS) were up-regulated by blood feeding. This suggests that this important bacterial transport system may be utilized to secrete effectors during the tick vector's blood meal. Blood feeding also up-regulated the expression of antioxidant enzymes, which might correspond to an attempt by R. rickettsii to protect itself against the deleterious effects of free radicals produced by fed ticks. The modulated genes identified in this study, including those encoding hypothetical proteins, require further functional analysis and may have potential as future targets for vaccine development.São Paulo Research Foundation (FAPESP) - 2008/053570-0Coordination for the Improvement of Higher Education Personnel (CAPES)National Council for Scientific and Technological Development (CNPq)The National Institutes of Science and Technology Program in Molecular Entomology (INCT-EM

    Efecto del extracto de las hojas de Gingko biloba en el crecimiento y la morfología de tripanosomátidos

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    Gingko biloba has been one of the most used medicinal plants all over the world in the past years. In this study, our group has studied the effect of a hydroethanolic extract from the aerial parts of this plant on the growth and morphological differentiation of trypanosomatids. Herpetomonas samuelpessoai and Herpetomonas sp were used in this study. The extract was obtained in a Soxhlet apparatus (50 oC, 2 hours). This extract was aseptically added to Roitman’s medium in different concentrations (4, 20, 40, 60, 80 and 100 mg/ml). The growth rate was determined using a Newbauer chamber to count numbers of cells after the extract inoculation (24 and 72 hours later). Smears stained by the Panotic method was used to determine the percentages of pro, para and opisthomastigote forms. The extract inhibited Herpetomonas sp growth in concentrations higher than 20 mg/ml. H. samuelpessoai has been inhibited in doses higher than 40 mg/ml. No morphological differentiation was observed in Herpetomonas sp cell. However, morphological differentiations could be noticed in H. samuelpessoai cell using doses higher than 40 mg/ml. These alterations are probably related to the cell division process, since cells with 3 or 4 nucleus were observed. Also, cytoplasmatic expansions, representing unsuccessful process of cell division were frequently found out. Further ultrastructural analysis using a transmission electron microscope showed cells with homogeneous nucleus or the absence of it. Protozoan protein profile was also analyzed. It was possible to notice changes in both trypanosomatids used in this study. H. samuelpessoai has shown over expression and accumulation of proteins which its degradation is essential to continue the cell differentiation. Also, it is possible to suggest that this extract acts through the modulation of the genetic expression and may be harmful to human cells if not purified.Gingko biloba es una de las plantas medicinales más utilizadas en todo el mundo en los últimos años. En este estudio, nuestro grupo ha estudiado el efecto de un extracto hidroetanólico de la parte aérea de esta planta sobre el crecimiento y la diferenciación morfológica de tripanosomátidos. Herpetomonas samuelpessoai y Herpetomonas sp se utilizaron en este estudio. El extracto se obtuvo en un aparato Soxhlet (50° C/2 horas). Este extracto se agregó asépticamente a medio Roitman en diferentes concentraciones (4, 20, 40, 60, 80 y 100 mg /ml). La tasa de crecimiento se determinó utilizando una cámara de Newbauer para contar el número de células después de la inoculación de extracto (24 y 72 horas más tarde). Frotis teñidos por el método Panotic se utilizó para determinar los porcentajes de pro, para y las formas opistomastigota. El extracto inhibió el crecimiento Herpetomonas sp en concentraciones superiores a 20 mg /ml. H. samuelpessoai se ha inhibido en dosis superiores a 40 mg /ml. No se observó diferenciación morfológica en la celda Herpetomonas sp. Sin embargo, las diferenciaciones morfológicas se pudo observar en la celda H. samuelpessoai con dosis superiores a 40 mg /ml. Estas alteraciones son probablemente relacionado con el proceso de división celular, ya que las células con 3 o 4 núcleos se observaron. Además, las expansiones citoplasmáticas, lo que representa el proceso fallido de la división celular se encontraron con frecuencia hacia fuera. Un análisis más detallado ultraestructural usando microscopio electrónico de transmisión mostró células con núcleo homogéneo o la ausencia de ella. El perfil de proteínas por Protozoarios también se ha analizado. Fue posible notar cambios tanto en tripanosomátidos utilizados en este estudio. H. samuelpessoai ha demostrado a lo largo de expresión y la acumulación de proteínas que su degradación es esencial para continuar con la diferenciación celular. Además, es posible sugerir que este extracto actúa a través de la modulación de la expresión genética

    Structural and productive features of Panicum cultivars submitted to different rest periods in the irrigated semiarid region of Brazil

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    The objective of this study was to evaluate the structural and productive features of Panicum maximum cultivars (Tanzânia, Mombaça, Massai, and Zuri) in three rest periods (30, 45 and 60 days), in the semiarid region of Brazil. The experiment was carried out in a completely randomized design with three replicates. The length of the leaf blade and the diameter of the stem increased as a function of the cut intervals. Leaf blade width and number of live leaves (3.86 leaves tiller-1) were not affected by cut intervals. The height of the canopy increased with the ages, with effect in the mass of forage, being the cv. Zuri the most productive with 148,75 cm e 18.297,49 kg ha-1 DM at 60 days. The highest masses of leaves and stems were obtained in the longest rest period, while the leaf/stem ratio decreased. In the cut-off interval of 45 days, the cultivars of Panicum maximum have shown satisfactory yield, and the smaller cut interval provides a reduction in canopy height and stem thickness
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