Suppressing cyanobacterial dominance by UV-LED TiO2-photocatalysis in a drinking water reservoir: a mesocosm study.

Cyanobacteria and their toxic secondary metabolites present challenges for water treatment globally. In this study we have assessed TiO2 immobilized onto recycled foamed glass beads by a facile calcination method, combined in treatment units with 365 nm UV-LEDs. The treatment system was deployed in mesocosms within a eutrophic Brazilian drinking water reservoir. The treatment units were deployed for 7 days and suppressed cyanobacterial abundance by 85%, while at the same time enhancing other water quality parameters; turbidity and transparency improved by 40 and 81% respectively. Genomic analysis of the microbiota in the treated mesocosms revealed that the composition of the cyanobacterial community was affected and the abundance of Bacteroidetes and Proteobacteria increased during cyanobacterial suppression. The effect of the treatment on zooplankton and other eukaryotes was also monitored. The abundance of zooplankton decreased while Chrysophyte and Alveolata loadings increased. The results of this proof-of-concept study demonstrate the potential for full-scale, in-reservoir application of advanced oxidation processes as complementary water treatment processes

Adubação nitrogenada de sorgo granífero consorciado com capim em sistema de plantio direto

O objetivo deste trabalho foi avaliar o efeito do manejo da adubação nitrogenada sobre a cultura do sorgo granífero, cultivado solteiro e em consócio com capim-marandu e capim-mombaça, e determinar a produtividade de matéria seca das forrageiras, nos anos agrícolas 2003/2004 e 2004/2005, em plantio direto. Foi utilizado o delineamento experimental em blocos ao acaso, em arranjo fatorial 3x5, com quatro repetições. Os tratamentos consistiram de três sistemas de cultivo de sorgo granífero (solteiro e consorciado com capim-marandu ou capim-mombaça, na linha de semeadura) e cinco manejos de adubação nitrogenada: 30-70; 70-30; 50-50; 100-0; e 0-100 kg ha-1 de N, quantidades aplicadas na semeadura e na cobertura, respectivamente. O cultivo consorciado não afetou a nutrição nem a produtividade de grãos de sorgo. Apenas no primeiro ano de cultivo, o parcelamento 50-50 kg ha-1 de N proporcionou maior produtividade de grãos. O manejo da palhada interferiu no estabelecimento do sorgo no segundo ano de cultivo, e diminuiu a produtividade de grãos. As maiores doses de N aplicadas em cobertura elevaram a produtividade de matéria seca do capim-marandu e, as aplicadas em semeadura, a elevaram no capim-mombaça

Development of an amplicon-based sequencing approach in response to the global emergence of mpox

The 2022 multicountry mpox outbreak concurrent with the ongoing Coronavirus Disease 2019 (COVID-19) pandemic further highlighted the need for genomic surveillance and rapid pathogen whole-genome sequencing. While metagenomic sequencing approaches have been used to sequence many of the early mpox infections, these methods are resource intensive and require samples with high viral DNA concentrations. Given the atypical clinical presentation of cases associated with the outbreak and uncertainty regarding viral load across both the course of infection and anatomical body sites, there was an urgent need for a more sensitive and broadly applicable sequencing approach. Highly multiplexed amplicon-based sequencing (PrimalSeq) was initially developed for sequencing of Zika virus, and later adapted as the main sequencing approach for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Here, we used PrimalScheme to develop a primer scheme for human monkeypox virus that can be used with many sequencing and bioinformatics pipelines implemented in public health laboratories during the COVID-19 pandemic. We sequenced clinical specimens that tested presumptively positive for human monkeypox virus with amplicon-based and metagenomic sequencing approaches. We found notably higher genome coverage across the virus genome, with minimal amplicon drop-outs, in using the amplicon-based sequencing approach, particularly in higher PCR cycle threshold (Ct) (lower DNA titer) samples. Further testing demonstrated that Ct value correlated with the number of sequencing reads and influenced the percent genome coverage. To maximize genome coverage when resources are limited, we recommend selecting samples with a PCR Ct below 31 Ct and generating 1 million sequencing reads per sample. To support national and international public health genomic surveillance efforts, we sent out primer pool aliquots to 10 laboratories across the United States, United Kingdom, Brazil, and Portugal. These public health laboratories successfully implemented the human monkeypox virus primer scheme in various amplicon sequencing workflows and with different sample types across a range of Ct values. Thus, we show that amplicon-based sequencing can provide a rapidly deployable, cost-effective, and flexible approach to pathogen whole-genome sequencing in response to newly emerging pathogens. Importantly, through the implementation of our primer scheme into existing SARS-CoV-2 workflows and across a range of sample types and sequencing platforms, we further demonstrate the potential of this approach for rapid outbreak response.This publication was made possible by CTSA Grant Number UL1 TR001863 from the National Center for Advancing Translational Science (NCATS), a component of the National Institutes of Health (NIH) awarded to CBFV. INSA was partially funded by the HERA project (Grant/ 2021/PHF/23776) supported by the European Commission through the European Centre for Disease Control (to VB).info:eu-repo/semantics/publishedVersio

Modeling of human exposure to benzene in urban environments

Urban areas characterized by high spatial and temporal variability in air pollution levels require implementation of comprehensive approaches to address exposure of individuals. The main objective of this study was to implement a quantitative assessment of individual exposure to benzene in urban environments. For this purpose, ExPOSITION model based on a global positioning system (GPS) tracking approach was applied to estimate individual exposure in different microenvironments. The current investigation provides an application example and validation of the modeling approach against personal and biological exposure measurements collected during the measurements campaign. The probabilistic approach using the Johnson system of distributions was implemented to characterize variability of indoor concentrations. The results obtained for daily average individual exposure to benzene corresponded to mean levels of 1.6 and 0.8–2.7 μg/m3 in terms of 5th–95th percentiles. Validation of the model results against several personal exposure samples collected for the selected individuals revealed a Pearson’s correlation coefficient of .66. This modeling approach explicitly addressed the temporal and spatial variability in the exposure and established a source–receptor relationship.The authors thank the Portuguese Foundation for Science and Technology for the financing of the PhD grant of D. Dias (SFRH/BD/47578/2008), the Polytechnic Institute of Leiria (IPL) for the availability of monitoring air quality equipment, and the volunteers who participated in the monitoring campaign for their willingness and dedication

Serum biochemical profile of Pêga breed donkeys in the state of Minas Gerais

ABSTRACT: For the evaluation of serum biochemical parameters of Pêga breed donkeys (Equus asinus), for the different age groups and sex, blood samples of 123 animals were analyzed, of 29 males aged from 8 days to 10 years and of 94 females (15 lactating) aged from 2 days to 12 years, from two farms in the central-southern Minas Gerais, Brazil. The donkeys were divided by age into 5 groups: Group 1 (≤6 months), Group 2 (7-12 months), Group 3 (13-48 months), Group 4 (49-72 months), and Group 5 (≥73 months). According to the sex, they were divided into two groups, males and females. Serum biochemical elements: total protein, albumin, globulin, the A:G ratio, cholesterol, triglycerides, uric acid, creatinine, urea, phosphorus, calcium, Ca:P ratio, magnesium, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT) and creatine kinase (CK), were evaluated in all animals. No significant differences were found for globulins, uric acid, urea and A:G ratio between age groups. Group 4 showed the highest values for total protein when compared with animals in Group 1 and 2. In Goup 2, the donkeys showed albumin levels lower than Group 3 and 4. Group 1 they had cholesterol levels higher than those in Group 2 and 4, and similar of the other groups. Higher phosphorus serum concentration was observed in Group 1. Calcium was significantly lower in Group 2. The Ca:P ratio was higher for Group 5. The magnesium values were significantly higher in donkeys older than 49 months (Group 4 and 5). The value of AST was lower for group 1. The ALP enzyme was significantly higher in younger animals up to 12 months, followed by gradual decrease with advancing age. The values of GGT were higher in donkeys up to 6 months, followed by decreasing values for subsequent groups. No differences were found between genders for albumin, cholesterol, creatinine, urea, uric acid, Ca:P ratio, magnesium, ALT, AST, and alkaline phosphatase. Females had higher values for total protein, globulin and triglycerides. Males showed higher values for A:G ratio, phosphorus, calcium and CK. The results showed that age and sex can influence serum biochemical values of Pêga breed donkeys