Responses of CYP450 dependent system to aliphatic and aromatic hydrocarbons body burden in transplanted mussels from South coast of Portugal

Mussels Mytilus galloprovincialis were cross-transplanted at South Portugal from a reference site (site 1) to a site more contaminated with hydrocarbon compounds (site 2), and vice versa, in an active biomonitoring (ABM) concept, to assess the biotransformation capacity catalyzed by the mixed function oxygenase (MFO) system. Total alkanes (TAlk), the unresolved complex mixture (UCM), and total polycyclic aromatic hydrocarbons (TPAHs) concentration increased respectively 6, 4.4 and 4.2 fold relatively to control, in mussels transplanted from site 1 to 2. In the cross-transplant, a 48, 57 and 62% depuration of TAlk, UCM and TPAHs concentrations occurred by the end of the 3-4th week. Petrogenic and biogenic (marine and terrigenous) sources of AHs, and petrogenic and pyrolitic (biomass and oil/fuel incomplete combustion) sources of PAHs were detected at both sites. CYP450, CYT b (5) and NADPH-RED in mussels transplanted from site 1 to 2 were induced from day 0 to 28, with a total increase of 35, 32 and 35%, respectively, while biochemical equilibrium to lesser environmental contamination occurs in mussels transplanted from site 2 to 1. A significant relationship between CYP450 and NADPH-RED was found with TPAH, with distinctive behavior at the two sites. MFO system components increase with exposure time at one site and decreases in the other, reflecting an adaptation to distinct environmental hydrocarbon loads. The ABM strategy proved to be useful to understand the environment real impact on the biochemical responses in mussels' local populations. In this study, CYP450 and NADPH-RED are a useful biomarker for hydrocarbon exposure.FCT: PRAXIS XXI/BD/3740/94info:eu-repo/semantics/publishedVersio

Biomarkers: a strategic tool in the assessment of environmental quality of coastal waters

Ecosystems are under the pressure of complex mixtures of contaminants whose effects are not always simple to assess. Biomarkers, acting as early warning signals of the presence of potentially toxic xenobiotics, are useful tools for assessing either exposure to, or the effects of these compounds providing information about the toxicant bioavailability. In fact, it has been argued that a full understanding of ecotoxicological processes must consider an integrated multi-level approach, in which molecular impact is related with higher-order biological consequences at the individual, population and community levels. Monitoring programs should make use of this tool to link contaminants and ecological responses fulfilling strategies like those launched by OSPAR (Commissions of Oslo and Paris) Convention on the protection of the marine environment of the North-East Atlantic and the International Council for the Exploration of the Sea (ICES). An overview of the work done in the past few years using biomarkers as in situ tools for pollution assessment in Portuguese coastal waters is presented as a contribution to the set up of a biomonitoring program for the Portuguese coastal zone. Considering the data set available the biomonitoring proposal should include the analysis of biomarkers and effects at individual levels. The aim of the program will include a spatial and temporal characterization of the biomarkers acetyl-cholinesterase, metallothioneins, DNA damage, adenylate energy charge and scope-for-growth levels. The investigation of the spatial variation of biomarkers is crucial to define sites for long term monitoring, which will be integrated with a chemical monitoring program. This framework will be a major contribution to the implementation of a national database for the use of biomarkers along the Portuguese coast.info:eu-repo/semantics/publishedVersio

Access networks for mobility: a techno-economic model for broadband access technologies

The two main challenges for the access networks are the increasing bandwidth demand and mobility trends. The "triple play" services required (Internet, telephone and TV services) lead to a great increase in bandwidth demand. However, the existing access networks are not able to support this increase, and the capacity to delivery broadband services remain as a challenge ("last mile problem"). The access network remains a bottleneck in terms of the bandwidth and service quality it affords the end user. Besides the bandwidth, other great challenge to access networks is the mobility and the user need to have internet access anywhere and anytime. Then, the increasing demand of "quad-play" (also known as quadruple-play) services, including video, voice, data and mobility, have created new challenges to the modern broadband wireless/wired access networks. This document proposes a techno-economic model to support the new requirements of fixed and nomadic users

Game theoretic modeling of NGANs: impact of retail and wholesale services price variation

The increasing demand for broadband access leads operators to upgrade the existing access infrastructures (or building new access network). Broadband access networks require higher investments (especially passive infrastructures such as trenches/ducts and base station towers/masts), and before making any decision it is important to analyze all solutions. The selection of the best solution requires understanding the technical possibilities and limitations of the different access technologies, as well as understanding the costs of building and operating the networks. This study analyzes the effect of asymmetric retail and wholesale prices on operators’ NPV, profit, consumer surplus, welfare, retail market, wholesale market, and so on. For that, we propose a tehno-economic model complemented by a theoretic-game model. This tool identifies all the essential costs of building (and operating) access networks, and performs a detailed analysis and comparison of the different solutions in various scenarios. Communities, operators/service providers, and regulators can use this tool to compare different technological solutions, forecast deployment costs, compare diferente scenarios, and so on, and help them in making deployment (or regulatory) decisions. The game-theory analyses give a better understanding of the competition and its effect on the business case scenarios’ economic results

Infrastructure sharing as an opportunity to promote competition in local access networks

Telecom infrastructures are facing unprecedented challenges, with increasing demands on network capacity. Today, network operators must determine how to expand the existing access network infrastructure into networks capable of satisfying the user’s requirements. Thus, in this context, providers need to identify the technological solutions that enable them to profitably serve customers and support future needs. However, the identification of the “best” solution is a difficult task. Although the cost of bandwidth in the active layer has reduced significantly (and continually) in recent years, the cost of the civil works—such as digging and trenching—represents a major barrier for operators to deploy NGA infrastructure. Duct is a critical part of the next-generation access networks, and its sharing would reduce or eliminate this capital cost and this barrier to entry. The aim of this paper is to provide a better understanding of the economics of broadband access networks technologies (wireline and wireless), their role in the deployment of several services in different regions, and the development of competition in the access networks

Plantas Hospedeiras de Cornops aquaticum (Bruner) (Orthoptera: Acrididae) no Pantanal de Poconé-MT

The grasshopper Cornops aquaticum (Bruner) lives permanently on aquatic macrophytes in floodplains of tropical South America. Its host plants and feeding preference were determined by field observations, crop analysis and feeding tests in the laboratory. Macrophytes were sampled and their petioles were examined for potential egg deposition in order to reveal host plant specificity. Sub-samples were dissected in the laboratory and eggs were counted directly whereas the rest of the sample was kept in net-covered water tanks aiming to verify hatching of nymphs. C. aquaticum fed and oviposited on Eichhornia azurea and Pontederia cordata (Pontederiaceae) in the field. Out of 140 fore-guts analyzed from free-living grasshoppers, 75% contained Pontederiaceae tissues. In the laboratory, however, it accepted plant species from other families. Tests of acceptance revealed that from 19 plant species offered, one by one, 16 were accepted. The seven plant species, which were accepted with highest frequency, were selected for tests of feeding rates. These rates were equal for all seven species. When four or five food plant species were offered simultaneously, the host species were not always preferred. The fact that in the laboratory more host-plant species were accepted than in the field indicates that the host range in the field is determined by other ecological factors than the plant chemistry. Host plant selection by C. aquaticum can be related to the relative abundance of the macrophyte species in the field and to the protection, which they offer against predation. Considering the emerging parts of the macrophytes, E. azurea represents the most abundant resource in the floodplains. Moreover, the cryptic coloration of the grasshopper on E. azurea lessens predation risk. That is why, apart from being potentially polyphagous, C. aquaticum presents a considerable oligophagy in the field, feeding on few species of Pontederiaceae.O gafanhoto Cornops aquaticum (Bruner) vive permanentemente sobre macrófitas aquáticas em áreas alagáveis. Suas plantas hospedeiras e sua preferência alimentar foram determinadas a partir de observações de campo, análise de conteúdo intestinal e testes alimentares em laboratório. A especificidade para oviposição foi estudada através da coleta de macrófitas cujos pecíolos potencialmente conteriam ovos desse gafanhoto. Parte da amostra foi dissecada em laboratório e o restante mantido em caixas d'água teladas para se verificar a eclosão de ninfas. C. aquaticum alimentou-se e ovipositou em Eichhornia azurea e Pontederia cordata (Pontederiaceae) no campo. Dos 140 intestinos analisados 75% continham tecidos de Pontederiaceae. No laboratório, entretanto, os gafanhotos aceitaram espécies de outras famílias. Os testes de aceitabilidade mostraram que foram aceitas 16 das 19 espécies oferecidas separadamente. As sete espécies de plantas aceitas com maior freqüência foram selecionadas para as medidas das taxas de alimentação. Não houve diferença significativa entre o consumo das mesmas pelos gafanhotos. Quando quatro ou cinco espécies foram oferecidas simultaneamente, as espécies hospedeiras do campo nem sempre foram preferidas. O fato de maior número de espécies de plantas ter sido aceito no laboratório é um bom indicativo de que o espectro de plantas hospedeiras deve estar sendo determinado por outros fatores ecológicos além da química da planta. A seleção de plantas hospedeiras por C. aquaticum pode estar relacionada principalmente à abundância relativa das espécies de macrófita no campo e à proteção contra predação que esta oferece. Se considerada apenas a parte emersa das macrófitas, E. azurea representava o recurso mais abundante nas áreas alagadas. Além disso, a coloração críptica do gafanhoto sobre esta espécie possivelmente diminui o risco de predação. Portanto, embora seja potencialmente polífago, C. aquaticum apresenta uma considerável oligofagia no campo, alimentando-se e ovipositando sobre três espécies da família Pontederiaceae.523533Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Entrepreneurship research: A bibliometric study of the EnANPADs 1997-2008

We have witnessed a fast growth in academic interest on entrepreneurship over the past two to three decades, although at disparate paces in different countries. A wealth of papers presented and published, books, dedicated journals, websites, professional and research groups have emerged accompanying this increased interest. It is thus important to understand what are these scholars studying. In this paper we examine the state of the art in entrepreneurship research in Brazil, by scrutinizing the entire track record of the papers presented at the EnANPAD over a twelve years period: 1997 -to 2008. The results of the bibliometric analysis revealed that entrepreneurship research maintains a broad spectrum of interests, focusing both on contextual, individual and process issues. Less visible is a unified theoretical background or the use of established theories foundational to other management disciplines. It is notable the increase in Brazilian entrepreneurship research, both empiric, theoretical and case-study based. We conclude with an umbrella discussion and suggestions for future research.entrepreneurship research, entrepreneur, bibliometric study, EnANPAD

Context aware advisor for public transportation

This paper presents an Android-based mobile app designed to provide real time context aware public transportation information and advice to its users through the combination of the user's preferences and geographic context with data retrieved from a public transportation information system called XTraN Passenger. Thus, this mobile app contributes to fulfill the necessities of the passengers, and also provides an incentive for people to use the public transportation infrastructure more frequently. The proposed mobile app allows the users to benefit from the access to real time public transportation data in a simple and intuitive way. The validation of the features and operation of the developed app was assessed with results from use cases and real-world experimental tests using public transportation data from a Brazilian bus fleet operator.This work is supported by FCT with the reference project UID/EEA/04436/2013, by FEDER funds through the COMPETE 2020 –Programa Operacional Competitividade e Internacionalização (POCI) with the reference project POCI-01-0145-FEDER-006941