Measurement of blood acetoacetate and β-hydroxybutyrate in an automatic analyser

g-hydroxybutyrate and acetoacetate as well as lactate and pyruvate are intermediary metabolites normally present in blood. The g-hydroxybutyrate/acetoacetate ratio is an expression of the mitochondrial oxido-reduction state. This ketone body ratio can provide a clue to diagnosis and metabolic status in congenital errors of the electron transport chain and pyruvate metabolism. The standardization of these analytical procedures improves the interpretation of the results helping in the difficult diagnosis of mitochondrial diseases in children. This study describes an adaptation to a Dimension R 2 L (Dade Behring, Newark, Delaware, USA) automatic analyser for a method to measure blood ketone bodies (g-hydroxybutyrate and acetoacetate). The method allows the metabolites to be measured directly in nondeproteinized plasma (fluoride/ethylenediaminetetraacetic acid). This adaptation simplifies the analytical procedure and limits the turnaround time to 20 minutes. With a sample volume of 200 μ l metabolite concentrations ranging from 12 to 1300 μ molL−1 of g-hydroxybutyrate and from 10 to 450 μ molL−1 of acetoacetate may be measured with a reliable analytical response

Continuous Positive Airway Pressure Treatment in Patients with Alzheimer's Disease: A Systematic Review

Background: Epidemiological studies have suggested a pathophysiological relationship between obstructive sleep apnea syndrome (OSAS) and Alzheimer's disease (AD). The aim of this study is to evaluate the treatment of obstructive sleep apnea with continuous positive airway pressure (CPAP) in AD and its relationship with neurocognitive function improvement. Methods: Systematic review conducted following PRISMA's statements. Relevant studies were searched in MEDLINE, PEDro, SCOPUS, PsycINFO, Web of Science, CINAHL and SportDicus. Original studies in which CPAP treatment was developel in AD patients have been included. Results: 5 studies, 3 RCTs (Randomized controlled trials) and 2 pilot studies. In all RCTs the CPAP intervention was six weeks; 3 weeks of therapeutic CPAP vs. 3 weeks placebo CPAP (pCPAP) followed by 3 weeks tCPAP in patients with AD and OSA. The two pilot studies conducted a follow-up in which the impact on cognitive impairment was measured. Conclusions: CPAP treatment in AD patients decreases excessive daytime sleepiness and improves sleep quality. There are indications that cognitive deterioration function measured with the Mini Mental Scale decreases or evolves to a lesser extent in Alzheimer's patients treated with CPAP. Caregivers observe stabilization in disease progression with integration of CPAP. More research is needed on the topic presented

Effective Reduction of Radiation Exposure during Cardiac Catheterization

Exposure to ionizing radiation during cardiac catheterization can have harmful consequences for patients and for the medical staff involved in the procedures. Minimizing radiation doses during the procedures is essential. We investigated whether fine-tuning the radiation protocol reduces radiation doses in the cardiac catheterization laboratory. In January 2016, we implemented a new protocol with reduced radiation doses in the Hospital de Jerez catheterization laboratory. We analyzed 170 consecutive coronary interventional procedures (85 of which were performed after the new protocol was implemented) and the personal dosimeters of the interventional cardiologists who performed the procedures. Overall, the low-radiation protocol reduced air kerma (dose of radiation) by 44.9% (95% CI, 18.4%–70.8%; P=0.001). The dose-area product decreased by 61% (95% CI, 30.2%– 90.1%; P <0.001) during percutaneous coronary interventions. We also found that the annual deep (79%, P=0.026) and shallow (62.2%, P=0.035) radiation doses to which primary operators were exposed decreased significantly under the low-radiation protocol. These dose reductions were achieved without increasing the volume of contrast media, fluoroscopy time, or rates of procedural complications, and without reducing the productivity of the laboratory. Optimizing the radiation safety protocol effectively reduced radiation exposure in patients and operators during cardiac catheterization procedures

Incremento de la motivación en el aprendizaje de enseñanzas informáticas mediante herramientas maker

La enseñanza de asignaturas del ámbito de la informática está experimentando un gran cambio. La motivación del alumnado se encuentra en proceso de una recesión. Ésta, históricamente, se ha relacionado con el alumnado, pero no es del todo correcto. Es obligación del profesor aportar argumentos en la docencia para facilitar esta motivación. En este trabajo se ahonda acerca de esta discusión, incidiendo en las posibles causas. Como caso práctico, se explica la metodología implementada en una asignatura impartida en dos centros (y ciudades) diferentes y con idéntico contenido. Esta asignatura pertenece a un grado compartido entre Universidad de Sevilla y otra universidad, impartiéndose en ambas sedes por igual y con un temario idéntico. Mientras ésta asignatura posee históricamente un enfoque más clásico, desde la Universidad de Sevilla se ha dado un giro en su metodología de docencia. Esta actualización de contenidos y aprendizaje basado en proyectos se detalla aquí, así como los resultados obtenidos.The teaching of subjects in the informatics field is undergoing a drastic change. Student motivation is experimenting a recession. Motivation historically has been associated to students, but that statement is not entirely correct. The obligation of providing arguments in order to motivate students is a part of the teachers work too. This paper delves into this discussion, focusing on the possible causes of these problems. As a practical matter, it delves into the teaching methodology implemented in a subject taught in two different centers (and cities), but with the same content. This subject is imparted in an engineering degree that is part of a joint project between the University of Seville and other one, so it is imparted in both sites equally and with an identical agenda. While this subject has a more classical approach at most universities, from the University of Seville its teachers have modified the teaching methodology. This content update, the project-based learning and the obtained results are detailed here

CAR FOLLOWING TECHNIQUES: THE ROLE OF THE HUMAN FACTOR RECONSIDERED

[EN] Engineering and psychophysiological car following models emerge in the late 1950s (Saifuzzaman & Zheng, 2014). Such models differ in their ground concepts and explanatory mechanisms, but both assume a fundamental tenet: following each other, drivers invariably attempt to couple, keeping safety distance. More recent models focus on the spontaneous emergence of traffic jams that results from the properties of a system of interacting vehicles (i.e., without bottlenecks). In an experimental setting Sugiyama et al., (2008) have successfully recreated the conditions that allow the observation of the typical soliton wave going backwards through several car clusters. When certain speed, density and inter-vehicular distance join, so do traffic jams. Some of us have built upon these and other factors (e.g., wave movement in nature) exploring the mathematical properties of a system with three incognita that also needs three variables to be solved (Melchor & Sánchez, 2014). Two canonical car-following techniques emerge as a consequence: Driving to keep safety Distance (DD) vs Inertia (DI). Also a basic question: can drivers actually understand and follow either way, or do they stick to a basic normative driving behavior? This paper summarizes the results after three experimental studies done with a driving simulator. Several performance measures from individual drivers (accelerations, decelerations, average speed, distance to leader, and so on) were taken. As an overall indicator, results consistently announce in the three studies that DI trips consume less fuel (about 20%) than DD ones.Blanch Micó, MT.; Lucas Alba, A.; Bellés Rivera, T.; Ferruz Gracia, AM.; Melchor-Galán, Ó.; Delgado Pastor, L.; Ruíz Jimenez, F.... (2016). CAR FOLLOWING TECHNIQUES: THE ROLE OF THE HUMAN FACTOR RECONSIDERED. En XII Congreso de ingeniería del transporte. 7, 8 y 9 de Junio, Valencia (España). Editorial Universitat Politècnica de València. 851-858. https://doi.org/10.4995/CIT2016.2015.3341OCS85185

Recent advances on dendrogeomorphological research applied to flood hazard analysis in Spain.

Estudios dendroecológicos para el análisis de regímentes torrenciales y avenida

Mejoras en la estimación de la frecuencia y magnitud de avenidas torrenciales mediante técnicas dendrogeomorfológicas

Para prevenir el riesgo de inundación, en el análisis científico de la peligrosidad asociada a las avenidas fluviales, clásicamente se han empleado métodos hidrológico-hidráulicos y, en menor medida, histórico-paleohidrológicos y geológico-geomorfológicos. Sin embargo, estas técnicas plantean enormes incertidumbres científicas por la disponibilidad de los datos de partida, su validez espacio-temporal, y su representatividad estadística. La Dendrogeomorfología es un conjunto de técnicas que, aprovechando fuentes de información registradas en las raíces, troncos y ramas de los árboles y arbustos ubicados en determinadas posiciones geomorfológicas (bancos de orilla, barras longitudinales, llanura de inundación, etc.), permite completar (e incluso suplir) el registro sistemático y paleohidrológico de avenidas torrenciales que han acontecido en esa corriente. En este artículo se propone investigar, se muestran los resultados-tipo y se discute sobre la aplicación y limitaciones de las fuentes de datos y los métodos científicos derivados del análisis dendrogeomorfológico. Para llevarlo a cabo, se sugiere una combinación de métodos de adquisición de datos en campo, estudios de laboratorio y análisis de datos en gabinete, con un plan de trabajo que contempla doce tareas o actividades: 1, caracterización geomorfológica; 2, caracterización florística; 3, muestreo de ejemplares: 4, adquisición de datos topográficos detallados; 5, preparación de las muestras; 6, conteo y medida de los anillos de crecimiento; 7, estudio anatómico e histológico; 8, sincronización de las series; 9, detección y datación de eventos; 10, modelación hidráulica de tramos; 11, análisis estadístico de caudales de avenida; y 12, cartografía de las áreas de peligrosidad por avenidas torrenciales y mapas de riesgo. - In order to prevent flood risks, scientific fluvial flood hazard analysis has been carried out traditionally with hydrologic and hydraulic methods, and secondarily, using palaeohydrological-historical and geological-geomorphological methods. Nonetheless, these techniques pose countless uncertainties due to the availability of the data, their spatio-temporal validity and their statistical representativeness. Dendrogeomorphology is a set of techniques that takes advantage of sources of information registered in roots, trunks and branches of trees or treelike bushes placed in certain geomorphological locations (such as banks, longitudinal bars, flood prone areas, etc.), that may be useful to complete the systematic registry or paleohydrologic data of torrential floods that have occurred in a certain stream. The aim of this paper is to research the usefulness and limitations of the data sources and methodologies derived from the dendrogeomorphological analysis. For accomplishing this objective a combination of methods is proposed, from data acquisition methods in field, laboratory studies, to data analysis. The schedule comprises twelve tasks: 1, geomorphologic characterization; 2, floristic characterization; 3, species sampling: 4, acquisition of detailed topographic data; 5, sample arrangement; 6, growth ring count and measurement; 7, hystologic and anatomic study; 8, series synchronization; 9, event detection and dating; 10, hydraulic reach modelling; 11, flow data statistical analysis; y 12, hazard and flood prone areas cartography and risk mapping

Towards a classificatión of dendrogeomorphological evidences and their utility in flood hazard analysis

Se analizan los diferentes parámetros dendrogeomorfológicos válidos para la estimación de riesgo

West Nile virus emergence in humans in Extremadura, Spain 2020

In Spain, the largest human West Nile virus (WNV) outbreak among humans was reported in 2020, constituting the second most important outbreak in Europe that season. Extremadura (southwestern Spain) was one of the affected areas, reporting six human cases. The first autochthonous human case in Spain was reported in Extremadura in 2004, and no other human cases were reported until 2020. In this work, we describe the first WNV human outbreak registered in Extremadura, focusing on the most important clinical aspects, diagnostic results, and control actions which followed. In 2020, from September to October, human WNV infections were diagnosed using a combination of molecular and serological methods (an in-house specific qRT-PCR and a commercial ELISA for anti-WNV IgM and IgG antibodies) and by analysing serum, urine, and/or cerebrospinal fluid samples. Serological positive serum samples were further tested using commercial kits against related flaviviruses Usutu and Tick-borne encephalitis in order to analyse serological reactivity and to confirm the results by neutralisation assays. In total, six cases of WNV infection (five with neuroinvasive disease and one with fever) were identified. Clinical presentation and laboratory findings are described. No viral RNA was detected in any of the analysed samples, but serological cross-reactivity was detected against the other tested flaviviruses. Molecular and serological methods for WNV detection in various samples as well as differential diagnosis are recommended. The largest number of human cases of WNV infection ever registered in Extremadura, Spain, occurred in 2020 in areas where circulation of WNV and other flaviviruses has been previously reported in humans and animals. Therefore, it is necessary to enhance surveillance not only for the early detection and implementation of response measures for WNV but also for other emerging flaviviruses that could be endemic in this area.This research was partially funded by the project PI19CIII/00014 from the Instituto de Salud Carlos III.S