A new schedule-based transit assignment model with travel strategies and supply uncertainties

This paper proposes a new scheduled-based transit assignment model. Unlike other schedule-based models in the literature, we consider supply uncertainties and assume that users adopt strategies to travel from their origins to their destinations. We present an analytical formulation to ensure that on-board passengers continuing to the next stop have priority and waiting passengers are loaded on a first-come-first-serve basis. We propose an analytical model that captures the stochastic nature of the transit schedules and in-vehicle travel times due to road conditions, incidents, or adverse weather. We adopt a mean variance approach that can consider the covariance of travel time between links in a space–time graph but still lead to a robust transit network loading procedure when optimal strategies are adopted. The proposed model is formulated as a user equilibrium problem and solved by an MSA-type algorithm. Numerical results are reported to show the effects of supply uncertainties on the travel strategies and departure times of passengers.postprin

The reliability-based stochastic transit assignment problem with elastic demand

This paper examines the reliability-based stochastic transit assignment problem with elastic demand. A Variational Inequality (VI) model for this problem is developed. The VI model considers capacity, waiting time and in-vehicle travel time as stochastic variables, and includes Spiess and Florian’s (1989) and de Cea and Fernández’s (1993) models as special cases. A reliability-based stochastic user equilibrium condition is defined to capture the route choice behavior of passengers. To illustrate the properties of the VI model, numerical studies were conducted on de Cea and Fernández’s (1993) network. The studies also show that Spiess and Florian’s and de Cea and Fernández’s models can overestimate the system performance substantially.postprin

Design and analysis of magnet proportioning for dual-memory machines

published_or_final_versio

Multi-Constellation GNSS Multipath Mitigation Using Consistency Checking

In a typical urban environment, a mixture of multipath-free, multipath-contaminated and non-line-of-sight (NLOS) propagated GNSS signals are received. The errors caused by multipath-contaminated and NLOS reception are the dominant source of reduced consumer-grade positioning accuracy in the urban environment. Many conventional receiver-based and antenna-based techniques have been developed to mitigate either multipath or NLOS reception with mixed success. Nevertheless, the positioning accuracy can be maximised based on the simple principle of selecting only those signals least contaminated by multipath and NLOS propagation to form the navigation solution. The advent of multi-constellation GNSS provides the opportunity to realise this technique that is potentially low-cost and effective for consumer-grade devices. It may also be implemented as an augmentation to other multipath mitigation techniques. The focus of this paper is signal selection by consistency checking, whereby measurements from different satellites are compared with each other to identify the NLOS and most multipath-contaminated signals. The principle of consistency checking is that multipath-contaminated and NLOS measurements produce a less consistent navigation solution than multipath-free measurements. RAIM-based fault detection operates on the same principle. Three consistency-checking schemes based on single-epoch least-squares residuals are assessed: single sweep, recursive checking and a hybrid version of the first two. Two types of weighting schemes are also considered: satellite elevation-based and signal C/N0-based weighting. The paper also discussed the different observables that may be used by a consistency-checking algorithm for different applications and their effect on detection sensitivity. Test results for the proposed algorithms are presented using data from both static positioning and stand-alone dynamic positioning experiments. The static data was collected using a pair of survey-grade multi-constellation GNSS receivers using both GPS and GLONASS signals at open sky and urban canyon locations, while the dynamic data was collected using a consumer-grade GPS/GLONASS receiver on a car in a mixed urban environment. Significant improvements in position domain are demonstrated using the weighted recursive methods in the open environments. However in the urban environments, there are insufficient directly received signals for the conventional RAIM-based signal selection to be effective all the time. Both positioning improvements and risky outliers are demonstrated. More advanced techniques have been identified for investigation in future research

Lhx1/5 control dendritogenesis and spine morphogenesis of Purkinje cells via regulation of Espin

published_or_final_versio

Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development

Peer reviewedPublisher PD

Climate Variability and Hemorrhagic Fever with Renal Syndrome Transmission in Northeastern China

Background: The transmission of hemorrhagic fever with renal syndrome (HFRS) is influenced by climatic variables. However, few studies have examined the quantitative relationship between climate variation and HFRS transmission. ---------- Objective: We examined the potential impact of climate variability on HFRS transmission and developed climate-based forecasting models for HFRS in northeastern China. ---------- Methods: We obtained data on monthly counts of reported HFRS cases in Elunchun and Molidawahaner counties for 1997–2007 from the Inner Mongolia Center for Disease Control and Prevention and climate data from the Chinese Bureau of Meteorology. Cross-correlations assessed crude associations between climate variables, including rainfall, land surface temperature (LST), relative humidity (RH), and the multivariate El Niño Southern Oscillation (ENSO) index (MEI) and monthly HFRS cases over a range of lags. We used time-series Poisson regression models to examine the independent contribution of climatic variables to HFRS transmission. ----------- Results: Cross-correlation analyses showed that rainfall, LST, RH, and MEI were significantly associated with monthly HFRS cases with lags of 3–5 months in both study areas. The results of Poisson regression indicated that after controlling for the autocorrelation, seasonality, and long-term trend, rainfall, LST, RH, and MEI with lags of 3–5 months were associated with HFRS in both study areas. The final model had good accuracy in forecasting the occurrence of HFRS. ---------- Conclusions: Climate variability plays a significant role in HFRS transmission in northeastern China. The model developed in this study has implications for HFRS control and prevention

Transgenic expression of the dicotyledonous pattern recognition receptor EFR in rice leads to ligand-dependent activation of defense responses

Plant plasma membrane localized pattern recognition receptors (PRRs) detect extracellular pathogen-associated molecules. PRRs such as Arabidopsis EFR and rice XA21 are taxonomically restricted and are absent from most plant genomes. Here we show that rice plants expressing EFR or the chimeric receptor EFR::XA21, containing the EFR ectodomain and the XA21 intracellular domain, sense both Escherichia coli- and Xanthomonas oryzae pv. oryzae (Xoo)-derived elf18 peptides at sub-nanomolar concentrations. Treatment of EFR and EFR::XA21 rice leaf tissue with elf18 leads to MAP kinase activation, reactive oxygen production and defense gene expression. Although expression of EFR does not lead to robust enhanced resistance to fully virulent Xoo isolates, it does lead to quantitatively enhanced resistance to weakly virulent Xoo isolates. EFR interacts with OsSERK2 and the XA21 binding protein 24 (XB24), two key components of the rice XA21-mediated immune response. Rice-EFR plants silenced for OsSERK2, or overexpressing rice XB24 are compromised in elf18-induced reactive oxygen production and defense gene expression indicating that these proteins are also important for EFR-mediated signaling in transgenic rice. Taken together, our results demonstrate the potential feasibility of enhancing disease resistance in rice and possibly other monocotyledonous crop species by expression of dicotyledonous PRRs. Our results also suggest that Arabidopsis EFR utilizes at least a subset of the known endogenous rice XA21 signaling components

The phylogenetically-related pattern recognition receptors EFR and XA21 recruit similar immune signaling components in monocots and dicots

During plant immunity, surface-localized pattern recognition receptors (PRRs) recognize pathogen-associated molecular patterns (PAMPs). The transfer of PRRs between plant species is a promising strategy for engineering broad-spectrum disease resistance. Thus, there is a great interest in understanding the mechanisms of PRR-mediated resistance across different plant species. Two well-characterized plant PRRs are the leucine-rich repeat receptor kinases (LRR-RKs) EFR and XA21 from Arabidopsis thaliana (Arabidopsis) and rice, respectively. Interestingly, despite being evolutionary distant, EFR and XA21 are phylogenetically closely related and are both members of the sub-family XII of LRR-RKs that contains numerous potential PRRs. Here, we compared the ability of these related PRRs to engage immune signaling across the monocots-dicots taxonomic divide. Using chimera between Arabidopsis EFR and rice XA21, we show that the kinase domain of the rice XA21 is functional in triggering elf18-induced signaling and quantitative immunity to the bacteria Pseudomonas syringae pv. tomato (Pto) DC3000 and Agrobacterium tumefaciens in Arabidopsis. Furthermore, the EFR:XA21 chimera associates dynamically in a ligand-dependent manner with known components of the EFR complex. Conversely, EFR associates with Arabidopsis orthologues of rice XA21-interacting proteins, which appear to be involved in EFR-mediated signaling and immunity in Arabidopsis. Our work indicates the overall functional conservation of immune components acting downstream of distinct LRR-RK-type PRRs between monocots and dicots