Characterization of the early fiber development gene, Ligon-lintless 1 (Li1), using microarray

AbstractCotton fiber length is a key factor in determining fiber quality in the textile industry throughout the world. Understanding the molecular basis of fiber elongation would allow for improvement of fiber length. Ligon-lintless 1 (Li1) is a monogenic dominant mutation that results in short fibers. This mutant provides an excellent model system to study the molecular mechanisms of cotton fiber elongation. Microarray technology and quantitative real time PCR (qRT-PCR) were used to evaluate differentially expressed genes (DEGs) in the Ligon-lintless 1 (Li1) mutant compared to the wild-type. Although the results showed only a few differentially expressed genes at −1, 3 and 7days post anthesis (DPA); at 5 DPA, there were 1915 DEGs, including 984 up-regulated genes and 931 down-regulated genes. The critical stage for early termination of Li1 fiber elongation was 5 DPA, as there were the most differentially expressed genes in this sample. The transcription factors and other proteins identified might contribute to understanding the molecular basis of early fiber elongation. Gene ontology analysis identified some key GO terms that impact the regulation of fiber development during early elongation. These results provide some fundamental information about the TFs that might provide new insight into understanding the molecular mechanisms governing cotton fiber development

Host A-to-I RNA editing signatures in intracellular bacterial and single-strand RNA viral infections

BackgroundMicrobial infection is accompanied by remodeling of the host transcriptome. Involvement of A-to-I RNA editing has been reported during viral infection but remains to be elucidated during intracellular bacterial infections.ResultsHerein we analyzed A-to-I RNA editing during intracellular bacterial infections based on 18 RNA-Seq datasets of 210 mouse samples involving 7 tissue types and 8 intracellular bacterial pathogens (IBPs), and identified a consensus signature of RNA editing for IBP infections, mainly involving neutrophil-mediated innate immunity and lipid metabolism. Further comparison of host RNA editing patterns revealed remarkable similarities between pneumonia caused by IBPs and single-strand RNA (ssRNA) viruses, such as altered editing enzyme expression, editing site numbers, and levels. In addition, functional enrichment analysis of genes with RNA editing highlighted that the Rab GTPase family played a common and vital role in the host immune response to IBP and ssRNA viral infections, which was indicated by the consistent up-regulated RNA editing of Ras-related protein Rab27a. Nevertheless, dramatic differences between IBP and viral infections were also observed, and clearly distinguished the two types of intracellular infections.ConclusionOur study showed transcriptome-wide host A-to-I RNA editing alteration during IBP and ssRNA viral infections. By identifying and comparing consensus signatures of host A-to-I RNA editing, our analysis implicates the importance of host A-to-I RNA editing during these infections and provides new insights into the diagnosis and treatment of infectious diseases

Optimizing robotic thyroid surgery: lessons learned from an retrospective analysis of 104 cases

BackgroundRobotic assistance in thyroidectomy is a developing field that promises enhanced surgical precision and improved patient outcomes. This study investigates the impact of the da Vinci Surgical System on operative efficiency, learning curve, and postoperative outcomes in thyroid surgery.MethodsWe conducted a retrospective cohort study of 104 patients who underwent robotic thyroidectomy between March 2018 and January 2022. We evaluated the learning curve using the Cumulative Sum (CUSUM) analysis and analyzed operative times, complication rates, and postoperative recovery metrics.ResultsThe cohort had a mean age of 36 years, predominantly female (68.3%). The average body mass index (BMI) was within the normal range. A significant reduction in operative times was observed as the series progressed, with no permanent hypoparathyroidism or recurrent laryngeal nerve injuries reported. The learning curve plateaued after the 37th case. Postoperative recovery was consistent, with no significant difference in hospital stay duration. Complications were minimal, with a noted decrease in transient vocal cord palsy as experience with the robotic system increased.ConclusionRobotic thyroidectomy using the da Vinci system has demonstrated a significant improvement in operative efficiency without compromising safety. The learning curve is steep but manageable, and once overcome, it leads to improved surgical outcomes and high patient satisfaction. Further research with larger datasets and longer follow-up is necessary to establish the long-term benefits of robotic thyroidectomy

Comparison of heavy-ion transport simulations: Collision integral with pions and Δ resonances in a box

We compare ten transport codes for a system confined in a box, aiming at improved handling of the production of $\Delta$ resonances and pions, which is indispensable for constraining high-density symmetry energy from observables such as the $\pi^-/\pi^+$ yield ratio in heavy-ion collisions. The system in a box is initialized with nucleons at saturation density and at 60 MeV temperature. The reactions $NN\leftrightarrow N\Delta$ and $\Delta\leftrightarrow N\pi$ are implemented, but the Pauli blocking and the mean-field potential are deactivated in the present comparison. Results are compared to those from the two reference cases of a chemically equilibrated ideal gas mixture and of the rate equation. In the results of the numbers of $\Delta$ and $\pi$, deviations from the reference values are observed in many codes, and they depend significantly on the size of the time step. These deviations are tied to different ways in ordering the sequence of collisions and decays, that take place in the same time step. Better agreements are seen in the reaction rates and the number ratios among the isospin species of $\Delta$ and $\pi$. These are, however, affected by the correlations, which are absent in the Boltzmann equation, but are induced by the way particle scatterings are treated in transport calculations. The uncertainty in the transport-code predictions of the $\pi^-/\pi^+$ ratio for the system initialized at n/p = 1.5, after letting the existing $\Delta$ resonances decay, is found to be within a few percent, which is sufficiently small so that it does not strongly impact constraining the high-density symmetry energy from heavy-ion collisions. Most of the sources of uncertainties have been understood, and individual codes may be further improved. This investigation will be extended in the future to heavy-ion collisions to ensure the problems identified here remain under control.Comment: 36 pages, 27 figures; a new Fig. 21 and revised results from some codes, achieving improved and consistent understandin

Local synergetic collaboration between Pd and local tetrahedral symmetric Ni oxide enables ultra-high-performance CO2 thermal methanation

Tetrahedral symmetric NiO2 and Pd respectively facilitate H2 splitting and CO2 to CO reduction and thus enable an ultra-high CH4 production yield performance in the epitaxial interfaces in the bimetallic NiO2@Pd NPs.</p

The hornwort genome and early land plant evolution

Hornworts, liverworts and mosses are three early diverging clades of land plants, and together comprise the bryophytes. Here, we report the draft genome sequence of the hornwort Anthoceros angustus. Phylogenomic inferences confirm the monophyly of bryophytes, with hornworts sister to liverworts and mosses. The simple morphology of hornworts correlates with low genetic redundancy in plant body plan, while the basic transcriptional regulation toolkit for plant development has already been established in this early land plant lineage. Although the Anthoceros genome is small and characterized by minimal redundancy, expansions are observed in gene families related to RNA editing, UV protection and desiccation tolerance. The genome of A. angustus bears the signatures of horizontally transferred genes from bacteria and fungi, in particular of genes operating in stress-response and metabolic pathways. Our study provides insight into the unique features of hornworts and their molecular adaptations to live on land

Elliptic flow of electrons from heavy-flavor hadron decays in Au+Au collisions at sNN=\sqrt{s_{\rm NN}} = 200, 62.4, and 39 GeV

We present measurements of elliptic flow ($v_2$) of electrons from the decays of heavy-flavor hadrons ($e_{HF}$) by the STAR experiment. For Au+Au collisions at $\sqrt{s_{\rm NN}} =$ 200 GeV we report $v_2$, for transverse momentum ($p_T$) between 0.2 and 7 GeV/c using three methods: the event plane method ($v_{2}${EP}), two-particle correlations ($v_2${2}), and four-particle correlations ($v_2${4}). For Au+Au collisions at $\sqrt{s_{\rm NN}}$ = 62.4 and 39 GeV we report $v_2${2} for $p_T< 2$ GeV/c. $v_2${2} and $v_2${4} are non-zero at low and intermediate $p_T$ at 200 GeV, and $v_2${2} is consistent with zero at low $p_T$ at other energies. The $v_2${2} at the two lower beam energies is systematically lower than at $\sqrt{s_{\rm NN}} =$ 200 GeV for $p_T < 1$ GeV/c. This difference may suggest that charm quarks interact less strongly with the surrounding nuclear matter at those two lower energies compared to $\sqrt{s_{\rm NN}} = 200$ GeV.Comment: Version accepted by PR