A Blue Tide in the Golden State: Ballot Propositions, Population Change, and Party Identification in California

Party identification is notoriously sticky, yet over the last three decades the California electorate has changed tremendously. A once red state has become one of the most reliable Democratic strongholds in the nation. What explains this change? One common explanation rests with population shifts and macropartisan trends. Another claims the combination of a rapidly expanding Latino electorate and a series of high-profile anti-immigrant ballot initiatives supported by the state\u27s Republicans drove partisan change in California. Building off of previous research, we seek to reconcile the differential impacts of these factors on the state\u27s Latinos and non-Hispanic Whites. Our analyses partially confirm and clarify previous findings regarding Latino partisan change, while directly challenging findings regarding partisan change among non-Hispanic Whites

A Blue Tide in the Golden State: Ballot Propositions, Population Change, and Party Identification in California

Party identification is notoriously sticky, yet over the last three decades the California electorate has changed tremendously. A once red state has become one of the most reliable Democratic strongholds in the nation. What explains this change? One common explanation rests with population shifts and macropartisan trends. Another claims the combination of a rapidly expanding Latino electorate and a series of high-profile anti-immigrant ballot initiatives supported by the state\u27s Republicans drove partisan change in California. Building off of previous research, we seek to reconcile the differential impacts of these factors on the state\u27s Latinos and non-Hispanic Whites. Our analyses partially confirm and clarify previous findings regarding Latino partisan change, while directly challenging findings regarding partisan change among non-Hispanic Whites

A perfused multi-well bioreactor platform to assess tumor organoid response to a chemotherapeutic gradient.

There is an urgent need to develop new therapies for colorectal cancer that has metastasized to the liver and, more fundamentally, to develop improved preclinical platforms of colorectal cancer liver metastases (CRCLM) to screen therapies for efficacy. To this end, we developed a multi-well perfusable bioreactor capable of monitoring CRCLM patient-derived organoid response to a chemotherapeutic gradient. CRCLM patient-derived organoids were cultured in the multi-well bioreactor for 7 days and the subsequently established gradient in 5-fluorouracil (5-FU) concentration resulted in a lower IC50 in the region near the perfusion channel versus the region far from the channel. We compared behaviour of organoids in this platform to two commonly used PDO culture models: organoids in media and organoids in a static (no perfusion) hydrogel. The bioreactor IC50 values were significantly higher than IC50 values for organoids cultured in media whereas only the IC50 for organoids far from the channel were significantly different than organoids cultured in the static hydrogel condition. Using finite element simulations, we showed that the total dose delivered, calculated using area under the curve (AUC) was similar between platforms, however normalized viability was lower for the organoid in media condition than in the static gel and bioreactor. Our results highlight the utility of our multi-well bioreactor for studying organoid response to chemical gradients and demonstrate that comparing drug response across these different platforms is nontrivial