MICROBIAL PROCESSING OF ORGANIC WASTE STREAMS INTO PHAs AND OTHER HIGH VALUE BIO-PRODUCTS

In the last years, economic and environmental concerns arose for oil shortage and climate change; for these reasons the scientific community focused on possible oil substitutes. In this perspectives, the production of new energy, materials and chemicals of non-fossil origin, could be based on biological resources such as biomasses. The efforts of the microbiology group of DAFNAE are mainly devoted to the exploitation of waste and residual biomasses for the production of high value bio-products such as polyhydroxyalkanoates (PHAs), bioethanol and biohydrogen. PHAs are today considered among the most promising substitutes for petrol-based plastics nevertheless their substitution over the conventional plastics is limited by their expensive manufacturing because of the costly raw materials used as carbon sources and the complex downstream phase of PHAs recovery from bacterial cells. Possible solutions could be i) the utilization of cheap wastes of agro-food origin as carbon sources and ii) the simplification of downstream purification processes. To these aims, Cupriavidus necator DSM545, a well-known PHAs accumulator, has been genetically modified in order to acquire the ability of metabolizing lactose from whey (dairy industry) or lipids (from slaughterhouse) and the capacity to produce nuclease to facilitate downstream processes. In the first case, the modified strains resulted able to grow using whey or lipids as carbon sources, accumulating up to 30 and 60% of PHAs, respectively. In the second case, the recombinant C. necator DSM 545 resulted in an effective decrease of viscosity of bacterial cells lysates, thus avoiding the use of costly commercial nucleases for an efficient downstream. Bioethanol is a fuel obtained from renewable resources and it could be a promising alternative to petrol fuels. First generation bioethanol is mainly produced from corn and sugarcane, thus conflicting with food and feed production . On the contrary, bioethanol from residual and lignocellulosic biomass has environmental impact lower than fossil fuels and would not threaten food supplies. Unfortunately, Saccharomyces cerevisiae, the yeast used for industrial bioethanol production, is not equipped with suitable hydrolytic activities and thus cannot directly utilize starchy and lignocellulosic wastes as feedstock without the use of commercial enzymes. Recent studies were focused to develop a \u201cConsolidated bioprocessing\u201d (CBP), approach where a single yeast is able to hydrolyse starch and lignocellulose and ferment the resulting sugars into ethanol. . With this purpose, novel and robust S. cerevisiae strains were recently engineered at DAFNAE to secrete efficient cellulases and amylases for the efficient saccharification and fermentation of starchy and cellulosic by-products up to 65 g/L ethanol

Population-level impact and herd effects following the introduction of human papillomavirus vaccination programmes: updated systematic review and meta-analysis

Background More than 10 years have elapsed since human papillomavirus (HPV) vaccination was implemented. We did a systematic review and meta-analysis of the population-level impact of vaccinating girls and women against human papillomavirus on HPV infections, anogenital wart diagnoses, and cervical intraepithelial neoplasia grade 2+ (CIN2+)to summarise the most recent evidence about the effectiveness of HPV vaccines in real-world settings and to quantify the impact of multiple age-cohort vaccination.Methods In this updated systematic review and meta-analysis, we used the same search strategy as in our previous paper. We searched MEDLINE and Embase for studies published between Feb 1, 2014, and Oct 11, 2018. Studies were eligible if they compared the frequency (prevalence or incidence) of at least one HPV-related endpoint (genital HPV infections, anogenital wart diagnoses, or histologically confirmed CIN2+) between pre-vaccination and post-vaccination periods among the general population and if they used the same population sources and recruitment methods before and after vaccination. Our primary assessment was the relative risk (RR) comparing the frequency (prevalence or incidence) of HPV-related endpoints between the pre-vaccination and post-vaccination periods. We stratified all analyses by sex, age, and years since introduction of HPV vaccination. We used random-effects models to estimate pooled relative risks.Findings We identified 1702 potentially eligible articles for this systematic review and meta-analysis, and included 65 articles in 14 high-income countries: 23 for HPV infection, 29 for anogenital warts, and 13 for CIN2+.After 5\u20138 years of vaccination, the prevalence of HPV 16 and 18 decreased significantly by 83% (RR 0\ub717, 95% CI 0\ub711\u20130\ub725) among girls aged 13\u201319 years, and decreased significantly by 66% (RR 0\ub734, 95% CI 0\ub723\u20130\ub749) among women aged 20\u201324 years. The prevalence of HPV 31, 33, and 45 decreased significantly by 54% (RR 0\ub746, 95% CI 0\ub733\u20130\ub766) among girls aged 13\u201319 years. Anogenital wart diagnoses decreased significantly by 67% (RR 0\ub733, 95% CI 0\ub724\u20130\ub746) among girls aged 15\u201319 years, decreased significantly by 54% (RR 0\ub746, 95% CI 0.36\u20130.60) among women aged 20\u201324 years, and decreased significantly by 31% (RR 0\ub769, 95% CI 0\ub753\u20130\ub789) among women aged 25\u201329 years. Among boys aged 15\u201319 years anogenital wart diagnoses decreased significantly by 48% (RR 0\ub752, 95% CI 0\ub737\u20130\ub775) and among men aged 20\u201324 years they decreased significantly by 32% (RR 0\ub768, 95% CI 0\ub747\u20130\ub798). After 5\u20139 years of vaccination, CIN2+ decreased significantly by 51% (RR 0\ub749, 95% CI 0\ub742\u20130\ub758) among screened girls aged 15\u201319 years and decreased significantly by 31% (RR 0\ub769, 95% CI 0\ub757\u20130\ub784) among women aged 20\u201324 years.Interpretation This updated systematic review and meta-analysis includes data from 60 million individuals and up to 8 years of post-vaccination follow-up. Our results show compelling evidence of the substantial impact of HPV vaccination programmes on HPV infections and CIN2+ among girls and women, and on anogenital warts diagnoses among girls, women, boys, and men. Additionally, programmes with multi-cohort vaccination and high vaccination coverage had a greater direct impact and herd effects

Synthesis and conformational analysis of galactose-derived bicyclic scaffolds

Two novel galactose-derived bicyclic acetamides were synthesized. The conformational behavior of these cyclic acetamides in aqueous solution was fully investigated. The NMR spectroscopic data suggests that the pyran ring interconverts between two (C-4(1), S-1(3)) or three (C-4(1), S-1(3) and C-1(4)) conformations and molecular modelling studies allowed the structures' geometries to be defined. Three different force fields were used (Amber*, MM3* and OPLSAA) and fitting procedures were employed to reproduce the experimental (3)J(H-H) coupling constants and NOE contacts. Evaluation of the results established which of these three well-known force fields were the most reliable in reproducing the experimental observations and should therefore be used for analogous design studies. ((c) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006

Estudio de la morfolog\ueda y aclimataci\uf3n de plantas de Laelia eyermaniana Rchb. f. generadas in vitro

A morphological study of plantlets of Laelia eyermaniana Rchb.f. cultured in vitro was carried out. Histologic preparations were made to observe different stages from seed germination until ex vitro acclimatization of plantlets. Photomicrographs showed various transitional stages. Seven relative stages of development were identified and described: seed, non-photosynthetic protocorm, photosynthetic protocorm, differentiated protocorm, plantlets with leafs, plantlets with leaves and roots, and plantlets with leaves and two roots, as well as two transitory phases: somatic embryogenic mass (SEM) and protocorm-like bodies (PLBs) obtained by indirect somatic embryogenesis. Stomatal leaf density doubled under ex vitro conditions. This study contributes to the knowledge of morphological development of L. eyermaniana during in vitro culture and ex vitro acclimatization.En este trabajo se hizo un seguimiento morfológico de plantas de Laelia eyermaniana Rchb. f. obtenidas in vitro. Se realizaron preparaciones histológicas para observar diferentes etapas, desde la germinación hasta la aclimatación ex vitro. Las fotomicrografías de las preparaciones mostraron varios estadios transitorios. Se identificaron y describieron siete estadios relativos de desarrollo: semilla, protocormo no fotosintético, protocormo fotosintético, protocormo en diferenciación, plántula con hojas, plántulas con hojas y raíz, plántula con hojas y dos raíces. Así como también, dos fases transitorias: masa embrionaria somática (MES) y cuerpos parecidos a protocormos (PLBs) por sus siglas en inglés, obtenidos por embriogénesis somática indirecta. La densidad estomática en las hojas se duplicó bajo condiciones ex vitro. Este estudio contribuye al conocimiento del desarrollo morfológico de L. eyermaniana durante su cultivo in vitro y aclimatación ex vitro

Short-term effects of particulate matter constituents on daily hospitalizations and mortality in five South-European cities: Results from the MED-PARTICLES project

Background: Few recent studies examined acute effects on health of individual chemical species in the particulate matter (PM) mixture, and most of them have been conducted in North America. Studies in Southern Europe are scarce. The aim of this study is to examine the relationship between particulate matter constituents and daily hospital admissions and mortality in five cities in Southern Europe. Methods: The study included five cities in Southern Europe, three cities in Spain: Barcelona (2003-2010), Madrid (2007-2008) and Huelva (2003-2010); and two cities in Italy: Rome (2005-2007) and Bologna (2011-2013). A case-crossover design was used to link cardiovascular and respiratory hospital admissions and total, cardiovascular and respiratory mortality with a pre-defined list of 16 PM10and PM2.5constituents. Lags 0 to 2 were examined. City-specific results were combined by random-effects meta-analysis. Results: Most of the elements studied, namely EC, SO42-, SiO2,Ca, Fe, Zn, Cu, Ti, Mn, V and Ni, showed increased percent changes in cardiovascular and/or respiratory hospitalizations, mainly at lags 0 and 1. The percent increase by one interquartile range (IQR) change ranged from 0.69% to 3.29%. After adjustment for total PM levels, only associations for Mn, Zn and Ni remained significant. For mortality, although positive associations were identified (Fe and Ti for total mortality; EC and Mg for cardiovascular mortality; and NO3-for respiratory mortality) the patterns were less clear. Conclusions: The associations found in this study reflect that several PM constituents, originating from different sources, may drive previously reported results between PM and hospital admissions in the Mediterranean area

A geroscience approach for Parkinson's disease: Conceptual framework and design of PROPAG-AGEING project

Advanced age is the major risk factor for idiopathic Parkinson's disease (PD), but to date the biological relationship between PD and ageing remains elusive. Here we describe the rationale and the design of the H2020 funded project "PROPAG-AGEING", whose aim is to characterize the contribution of the ageing process to PD development. We summarize current evidences that support the existence of a continuum between ageing and PD and justify the use of a Geroscience approach to study PD. We focus in particular on the role of inflammaging, the chronic, low-grade inflammation characteristic of elderly physiology, which can propagate and transmit both locally and systemically. We then describe PROPAG-AGEING design, which is based on the multi-omic characterization of peripheral samples from clinically characterized drug-naive and advanced PD, PD discordant twins, healthy controls and "super-controls", i.e. centenarians, who never showed clinical signs of motor disability, and their offspring. Omic results are then validated in a large number of samples, including in vitro models of dopaminergic neurons and healthy siblings of PD patients, who are at higher risk of developing PD, with the final aim of identifying the molecular perturbations that can deviate the trajectories of healthy ageing towards PD development

Difficult tracheal intubation in neonates and infants. NEonate and Children audiT of Anaesthesia pRactice IN Europe (NECTARINE): a prospective European multicentre observational study

International audienceBackground: Neonates and infants are susceptible to hypoxaemia in the perioperative period. The aim of this study was to analyse interventions related to anaesthesia tracheal intubations in this European cohort and identify their clinical consequences.Methods: We performed a secondary analysis of tracheal intubations of the European multicentre observational trial (NEonate and Children audiT of Anaesthesia pRactice IN Europe [NECTARINE]) in neonates and small infants with difficult tracheal intubation. The primary endpoint was the incidence of difficult intubation and the related complications. The secondary endpoints were the risk factors for severe hypoxaemia attributed to difficult airway management, and 30 and 90 day outcomes.Results: Tracheal intubation was planned in 4683 procedures. Difficult tracheal intubation, defined as two failed attempts of direct laryngoscopy, occurred in 266 children (271 procedures) with an incidence (95% confidence interval [CI]) of 5.8% (95% CI, 5.1-6.5). Bradycardia occurred in 8% of the cases with difficult intubation, whereas a significant decrease in oxygen saturation (SpO2<90% for 60 s) was reported in 40%. No associated risk factors could be identified among co-morbidities, surgical, or anaesthesia management. Using propensity scoring to adjust for confounders, difficult anaesthesia tracheal intubation did not lead to an increase in 30 and 90 day morbidity or mortality.Conclusions: The results of the present study demonstrate a high incidence of difficult tracheal intubation in children less than 60 weeks post-conceptual age commonly resulting in severe hypoxaemia. Reassuringly, the morbidity and mortality at 30 and 90 days was not increased by the occurrence of a difficult intubation event