Doctor of Philosophy

dissertationPrevious studies of minority political behavior have demonstrated that empowerment, as measured by the election of a minority person to public office, has positive effects on participation among the members of the minority community. Although the empowerment theory has yet to be applied to American Indians, it shows much promise in explaining participation rates among this minority group because of the theory's emphasis on political context; and attitudinal factors. This dissertation explored the role of empowerment on American Indian participation, first by comparing turnout prior to empowerment to turnout post empowerment in three counties in the West: San Juan County, Utah; Big Horn County, Montana; and Roosevelt County, Montana. The findings indicate that turnout among Indians after empowerment, as defined by an Indian holding elected office, was higher than turnout prior to empowerment because of the positive effect of empowerment on perceptions and attitudes among American Indians. The election of an Indian to county office was a major context;ual change in each of the three counties, and the change had a positive impact on voter participation among Indians by influencing perceptions of government and attitudes of American Indians. Furthermore, the positive effect of empowerment on American Indian voters is both immediate and long-lasting. The positive effect on American Indian political behavior is evident immediately after empowerment, that is, Indians vote at higher rates in the first election following empowerment than prior to empowerment. Turnout continues to increase over time for American Indians, in contrast to non-Indian populations, indicating the long-lasting, positive effects of empowerment on Indian political behavior

States Taking Charge: Examining the Role of Race, Party Affliation, and Preemption in the Development of in-state tuition laws for undocumented immigrant students

Part I of this Article details both the legislative and legal history of undocumented immigrants’ access to education in the United States. Part II then describes the current U.S. state laws in effect regarding in-state tuition for undocumented immigrant students at state-funded colleges and universities. Part III further explores the development of laws and policies with a keen focus on potential correlations between (1) the racial composition of state legislatures and the passage of in-state tuition policies; (2) the race of governors and the passage of in-state tuition policies; (3) partisan composition of state legislatures and the passage of in-state tuition policies; and (4) party affiliation of governors and in-state tuition policies. Part IV describes the concept of preemption and discusses the extent to which preemption has impacted the state statutes identified in Part II of this Article. Finally, Part V discusses the practical and normative implications of this research

States Taking Charge: Examining the Role of Race, Party Affliation, and Preemption in the Development of in-state tuition laws for undocumented immigrant students

Part I of this Article details both the legislative and legal history of undocumented immigrants’ access to education in the United States. Part II then describes the current U.S. state laws in effect regarding in-state tuition for undocumented immigrant students at state-funded colleges and universities. Part III further explores the development of laws and policies with a keen focus on potential correlations between (1) the racial composition of state legislatures and the passage of in-state tuition policies; (2) the race of governors and the passage of in-state tuition policies; (3) partisan composition of state legislatures and the passage of in-state tuition policies; and (4) party affiliation of governors and in-state tuition policies. Part IV describes the concept of preemption and discusses the extent to which preemption has impacted the state statutes identified in Part II of this Article. Finally, Part V discusses the practical and normative implications of this research

Novel hydrogel system eliminates subculturing and improves retention of nonsenescent mesenchymal stem cell populations

Aim: To compare the physiological behavior of mesenchymal stem/stromal cells (MSCs) within an expandable tissue-mimetic 3D system relative to in vitro expansion in a traditional 2D system. Methods: Adipose-derived MSCs (ASCs) were continuously cultured for 6 weeks on either 2D culture plastic or in a 3D hydrogel system that eliminated subculturing. ASCs were assessed for senescence, ‘stem-like’ MSC markers, and ability for their secretome to augment a secondary cell population. Results: The 3D hydrogel system resulted in an enhanced retention of more regenerative, nonsenescent ASC populations that exhibited increased expression of ‘stem-like’ MSC surface markers. Conclusion: This study introduces a proof-of-concept design for a novel modular 3D system that can improve in vitro expansion of stem-like cell populations for future regenerative therapies

Tailoring the secretome composition of mesenchymal stem cells to augment specific functions of epidermal regeneration: an in vitro diabetic model

Introduction: Wound healing consists of a dynamic series of events that are highly dependent on paracrine factors for proper progression through the phases of wound healing. Inappropriate progression through the phases is associated with insufficient epidermal regeneration (i.e., re-epithelialization) of wounds and subsequent propagation of chronic wounds, such as diabetic ulcers, which are associated with increased patient morbidity. Recently, investigation into the dynamic secretome of Adipose-derived Mesenchymal Stem Cells (ASCs), have shown promise in augmenting the wound healing response of chronic diabetic wounds. However, currently utilized 2D culture techniques are known to drastically alter the regenerative phenotype of ASCs. In this study a novel tissue-mimetic 3D system was utilized as a means to culture ASCs. Methods: The capacity for the ASC secretome to augment epidermal regeneration activity was then evaluated after exposure of ASCs to “wound priming stimuli” in 2D and 3D. The priming stimuli consisted of coating the 2D and 3D systems with the wound matrix proteins, collagen type I, fibronectin, and fibrin. To understand the potential benefit of the ASC secretome in the context of diabetic wounds, keratinocytes (KCs) were exposed to super-physiological glucose levels to induce a diabetic-like phenotype (idKCs). Results: Relative to KCs, idKC exhibited a 52% and 23% decline in proliferation and migration, respectively. Subsequently, analyses of the ASC secretome were performed. ASC conditioned media (ASC-CM) from tissue-mimetic culture demonstrated a > 50% increase secretion of proteins and a 2-fold increase in secreted EVs, relative to 2D culture. Interestingly, the different priming stimuli did not alter the total amount of protein or EVs secreted within the tissue-mimetic system. However, evaluation of specific soluble proteins via ELISA revealed significant differences in key epidermal regeneration factors, such as EGF, IGF-1, FGF-2, MMP-1, TIMP-1, and TGFβ-1. Additionally, the relative effect of ASC-EVs from the 2D and 3D system on idKCs epidermal regeneration functionality varied significantly, with EVs from 3D-Collagen culture providing the most significant benefit on idKC activity. Discussion: Together, these data support the utilization of tissue-mimetic culture system to enhance the adaptability and secretory activity of MSC-like populations in order to generate tailored biologics, via priming stimuli, for specific wound healing applications

Exploration-Related Research on ISS: Connecting Science Results to Future Missions

In January, 2004, the U.S. President announced The Vision for Space Exploration, and charged the National Aeronautics and Space Administration (NASA) with using the International Space Station (ISS) for research and technology targeted at supporting U.S. space exploration goals. This paper describes: What we have learned from the first four years of research on ISS relative to the exploration mission; The on-going research being conducted in this regard; and Our current understanding of the major exploration mission risks that the ISS can be used to address. Specifically, we discuss research carried out on the ISS to determine the mechanisms by which human health is affected on long-duration missions, and to develop countermeasures to protect humans from the space environment. These bioastronautics experiments are key enablers of future long duration human exploration missions. We also discuss how targeted technological developments can enable mission design trade studies. We discuss the relationship between the ultimate number of human test subjects available on the ISS to the quality and quantity of scientific insight that can be used to reduce health risks to future explorers. We discuss the results of NASA's efforts over the past year to realign the ISS research programs to support a product-driven portfolio that is directed towards reducing the major risks of exploration missions. The fundamental challenge to science on ISS is completing experiments that answer key questions in time to shape design decisions for future exploration. In this context, exploration relevant research must do more than be conceptually connected to design decisions - it must become a part of the mission design process