Intonation and discourse processing

This paper describes intonational cues to discourse structure, and the role that intonation plays in spoken discourse processing. We begin by discussing two main structures in discourse that one must consider when doing research on discourse processing: segmentation and information status. We then review a number of key studies from the phonetics literature which have investigated the intonational marking of these structures. Next, we discuss in detail the psycholinguistic research to date which has examined the role that intonation can play in facilitating or inhibiting the processing of discourse in English and other related languages. We conclude by outlining directions for future research in the area of intonation-discourse processing

The Neuropsychological Profiles of Mild Alzheimer's Disease and Questionable Dementia as Compared to Age-Related Cognitive Decline

Test scores from a comprehensive neuropsychological battery administered to 1602 subjects consisting of 1347 subjects with probable Alzheimer's disease (AD), 100 subjects with questionable dementia (QD) and 155 non-demented elderly control subjects were cross-sectionally analyzed. Subjects with probable AD were categorized as mild (n = 244), moderate (n = 480), severe (n = 376), and very severe (n = 247) according to modified mini mental status exam (mMMSE) scores. Mean scores on individual neuropsychological tests are provided for each group of subjects. Stratified random sampling was performed to select a sample of mild AD subjects who were matched in age and education to non-demented elderly controls, and analyses focused on the performance of QD subjects and mild AD subjects, whose scores were compared to those of the elderly control subjects. Selected scores were organized by cognitive domain and logistic regressions were used to determine the domains and individual tests within each that were most predictive of group status. Results suggested a profile of scores associated with QD and mild AD including impaired recall of verbal information for both groups. Areas of lower functioning in QD subjects as compared to elderly controls included category fluency and visuospatial ability

The Effect of Mass Ratio on the Morphology and Time-scales of Disc Galaxy Mergers

The majority of galaxy mergers are expected to be minor mergers. The observational signatures of minor mergers are not well understood, thus there exist few constraints on the minor merger rate. This paper seeks to address this gap in our understanding by determining if and when minor mergers exhibit disturbed morphologies and how they differ from the morphology of major mergers. We simulate a series of unequal-mass moderate gas-fraction disc galaxy mergers. With the resulting g-band images, we determine how the time-scale for identifying galaxy mergers via projected separation and quantitative morphology (the Gini coefficient G, asymmetry A, and the second-order moment of the brightest 20% of the light M20) depends on the merger mass ratio, relative orientations and orbital parameters. We find that G-M20 is as sensitive to 9:1 baryonic mass ratio mergers as 1:1 mergers, with observability time-scales ~ 0.2-0.4 Gyr. In contrast, asymmetry finds mergers with baryonic mass ratios between 4:1 and 1:1 (assuming local disc galaxy gas-fractions). Asymmetry time-scales for moderate gas-fraction major disc mergers are ~ 0.2-0.4 Gyr, and less than 0.06 Gyr for moderate gas-fraction minor mergers. The relative orientations and orbits have little effect on the time-scales for morphological disturbances. Observational studies of close pairs often select major mergers by choosing paired galaxies with similar luminosities and/or stellar masses. Therefore, the various ways of finding galaxy mergers (G-M20, A, close pairs) are sensitive to galaxy mergers of different mass ratios. By comparing the frequency of mergers selected by different techniques, one may place empirical constraints on the major and minor galaxy merger rates.Comment: 16 pages; resubmitted to MNRA

From the Cover: Assignment of an Essential Role for the Neurospora Frequency Gene in Circadian Entrainment to Temperature Cycles

Circadian systems include slave oscillators and central pacemakers, and the cores of eukaryotic circadian clocks described to date are composed of transcription and translation feedback loops (TTFLs). In the model system Neurospora, normal circadian rhythmicity requires a TTFL in which a White Collar complex (WCC) activates expression of the frequency (frq) gene, and the FRQ protein feeds back to attenuate that activation. To further test the centrality of this TTFL to the circadian mechanism in Neurospora, we used low-amplitude temperature cycles to compare WT and frq-null strains under conditions in which a banding rhythm was elicited. WT cultures were entrained to these temperature cycles. Unlike those normal strains, however, frq-null mutants did not truly entrain to the same cycles. Their peaks and troughs always occurred in the cold and warm periods, respectively, strongly suggesting that the rhythm in Neurospora lacking frq function simply is driven by the temperature cycles. Previous reports suggested that a FRQ-less oscillator (FLO) could be entrained to temperature cycles, rather than being driven, and speculated that the FLO was the underlying circadian-rhythm generator. These inferences appear to derive from the use of a phase reference point affected by both the changing waveform and the phase of the oscillation. Examination of several other phase markers as well as results of additional experimental tests indicate that the FLO is, at best, a slave oscillator to the TTFL, which underlies circadian rhythm generation in Neurospora

Heterologous production of the insecticidal pea seed albumin PA1 protein by Pichia pastoris and protein engineering to potentiate aphicidal activity via fusion to snowdrop lectin Galanthus nivalis agglutinin; GNA)

BackgroundNew bioinsecticides with novel modes of action are urgently needed to minimise the environmental and safety hazards associated with the use of synthetic chemical pesticides and to combat growing levels of pesticide resistance. The pea seed albumin PA1b knottin peptide is the only known proteinaceous inhibitor of insect vacuolar adenosine triphosphatase (V-ATPase) rotary proton pumps. Oral toxicity towards insect pests and an absence of activity towards mammals makes Pa1b an attractive candidate for development as a bioinsecticide. The purpose of this study was to investigate if Pichia pastoris could be used to express a functional PA1b peptide and if it’s insecticidal activity could be enhanced via engineering to produce a fusion protein comprising the pea albumin protein fused to the mannose-specific snowdrop lectin (Galanthus nivalis agglutinin; GNA).ResultsWe report the production of a recombinant full-length pea albumin protein (designated PAF) and a fusion protein (PAF/GNA) comprised of PAF fused to the N-terminus of GNA in the yeast Pichia pastoris. PAF was orally toxic to pea (Acyrthosiphon pisum) and peach potato (Myzus persicae) aphids with respective, Day 5 LC50 values of 54 µM and 105 µM derived from dose–response assays. PAF/GNA was significantly more orally toxic as compared to PAF, with LC50 values tenfold (5 µM) and 3.3-fold (32 µM) lower for pea and peach potato aphids, respectively. By contrast, no phenotypic effects were observed for worker bumble bees (Bombus terristrus) fed PAF, GNA or PAF/GNA in acute toxicity assays. Confocal microscopy of pea aphid guts after pulse-chase feeding fluorescently labelled proteins provides evidence that enhanced efficacy of the fusion protein is attributable to localisation and retention of PAF/GNA to the gut epithelium. In contact assays the fusion protein was also found to be significantly more toxic towards A. pisum as compared to PAF, GNA or a combination of the two proteins.ConclusionsOur results suggest that GNA mediated binding to V-type ATPase pumps acts to potentiate the oral and contact aphicidal activity of PAF. This work highlights potential for the future commercial development of plant protein-based bioinsecticides that offer enhanced target specificity as compared to chemical pesticides, and compatibility with integrated pest management strategies

Geospatial statistics elucidate competing geological controls on natural CO2 seeps in Italy

Site selection for the geological storage of CO 2 for long timespans requires an understanding of the controls on containment, migration, and surface seepage of subsurface CO 2 fluids. Evidence of natural CO 2 migration from depth to the surface is documented at 270 sites from Italy, a prolific CO 2 province. Previous studies indicate that CO 2 delivery to and from buried structures that host CO 2 accumulations is fault controlled but competing controls on the CO 2 flow pathways affect the location and style of CO 2 release. Here, we conduct a meta-analysis using a novel geospatial approach to statistically determine the relationship between the geological setting and structures and the CO 2 seep spatial distribution and characteristics (morphological type, flux, and temperature) in Central Italy. We find that seep distribution differs on two spatial scales corresponding to the geological setting. On large scales (>5 km), seeps are isotropically distributed and align with regional structures such as anticlines, decollements, and extensional faults. On local scales (<5 km), seeps cluster and align with subsidiary geologic structures, including faults and lithological boundaries. The detailed location and flux of seeps within clusters are influenced by the regional structural domain: in the Tyrrhenian, seeps tend to be located along fault traces, whereas seeps are located as springs in the tip and ramp regions of fault scarps in the Apennines. Thus, our geospatial approach evidences, at a regional scale, how macrocrustal fluid flow is governed by deep extensional and compressional features but once CO 2 reaches shallower structures, it evidences how smaller scale features and hydrogeological factors distribute the CO 2 fluids in the near surface, dependent on the geological setting. This work not only demonstrates useful application of a novel geospatial approach to characterize competing crustal controls on CO 2 flow at different scales but also informs the design of appropriate site characterization and surface monitoring programs at engineered carbon stores

Cognitive Test Performance among Nondemented Elderly African Americans and Whites

We examined the neuropsychological test performance of a randomly selected community sample of English-speaking non-Hispanic African American and white elders in northern Manhattan. All participants were diagnosed as nondemented by a neurologist, whose assessment was made independent of neuropsychological test scores. African American elders obtained significantly lower scores on measures of verbal and nonverbal learning and memory, abstract reasoning, language, and visuospatial skill than whites. After using a stratified random sampling technique to match groups on years of education, many of the discrepancies became nonsignificant; however, significant ethnic group differences on measures of figure memory, verbal abstraction, category fluency, and visuospatial skill remained. Discrepancies in test performance of education-matched African Americans and whites could not be accounted for by occupational attainment or history of medical conditions such as hypertension and diabetes. These findings emphasize the importance of using culturally appropriate norms when evaluating ethnically diverse elderly for dementia

CANDELS: The correlation between galaxy morphology and star formation activity at z~2

We discuss the state of the assembly of the Hubble Sequence in the mix of bright galaxies at redshift 1.4< z \le 2.5 with a large sample of 1,671 galaxies down to H_{AB}~26, selected from the HST/ACS and WFC3 images of the GOODS--South field obtained as part of the GOODS and CANDELS observations. We investigate the relationship between the star formation properties and morphology using various parametric diagnostics, such as the Sersic light profile, Gini (G), M_{20}, Concentration (C), Asymmetry (A) and multiplicity parameters. Our sample clearly separates into massive, red and passive galaxies versus less massive, blue and star forming ones, and this dichotomy correlates very well with the galaxies' morphological properties. Star--forming galaxies show a broad variety of morphological features, including clumpy structures and bulges mixed with faint low surface brightness features, generally characterized by disky-type light profiles. Passively evolving galaxies, on the other hand, very often have compact light distribution and morphology typical of today's spheroidal systems. We also find that artificially redshifted local galaxies have a similar distribution with z~2galaxies in a G-M_{20} plane. Visual inspection between the rest-frame optical and UV images show that there is a generally weak morphological k-correction for galaxies at z~2, but the comparison with non-parametric measures show that galaxies in the rest-frame UV are somewhat clumpier than rest-frame optical. Similar general trends are observed in the local universe among massive galaxies, suggesting that the backbone of the Hubble sequence was already in place at z~2.Comment: 22 pages, 19 figures, ApJ accepted (added 3 references