Quantifying and understanding current and future links between tropical convection and the large-scale circulation

2020 Fall.Includes bibliographical references.Tropical deep convection plays an important role in the variability of the global circulation. The Madden Julian Oscillation (MJO) is a large tropical organized convective system that propagates eastward along the equator. It is a key contributor to weather predictability at extended time scales (10-40 days). For example, variability in the MJO is linked with variability in meteorological phenomena such as landfalling atmospheric rivers, tornado and hail activity over parts of North America, and extreme temperature and rainfall patterns across the Northern Hemisphere. Links between the MJO and atmospheric variability in remote locations are heavily studied. This is in part because the current skill of weather forecasts at extended time scales is mediocre, and because of evidence suggesting that the potential predictability offered by the MJO may not be fully captured in numerical prediction models. In the first part of this dissertation, I develop a tool for these types of studies. The "Sensitivity to the Remote Influence of Periodic Events" (STRIPES) index is a novel index that condenses the information obtained through composite analysis of variables after a periodic event (such as the MJO) into a single number, which includes information about the life cycle of the event, and for a range of lags with respect to each stage of the event. I apply the STRIPES index to surface observations and show that the MJO signal is detectable and significant at the level of individual weather stations over many parts of North America, and that the maximum strength of this signal exhibits regionality and seasonality. Tropical convection affects the extratropics primarily through the excitation of Rossby waves at the places where the upper-tropospheric divergent outflow associated with deep convection interacts with the background wind. In a future warmer climate, the strength of the mean circulation and convective mass flux is expected to weaken. A potential consequence is a weakening of Rossby wave excitation by tropical convective systems such as the MJO. In the second part of this study, I analyze a set of idealized simulations with specified surface warming and superparameterized convection and develop a framework to better understand why the mean circulation weakens with warming. I show that the decrease in the strength of the mean circulation can be explained by the slow rate at which atmospheric radiative cooling intensifies relative to the comparatively fast rate that the tropical dry static stability increases. I also show that despite a decrease in the mean convective mass flux, the warming tendency of the convective mass flux over the most deeply- convecting regions is not constrained to follow that of the global mean. In the final part of this dissertation, I consider how changes in the MJO and of the mean atmospheric state due to warming from increases in greenhouse gas concentrations may lead to changes in the MJO's impact over the North Pacific and North America. Specifically, I show that changes to the atmosphere's mean state dry static energy and winds have a larger impact on the MJO teleconnection than changes to MJO intensity and propagation characteristics

Barium and lithium in foraminifera: glacial-interglacial changes in the North Atlantic

The trace element content of calcareous foraminifera provides a powerful tool to the study of glacial-interglacial changes in the physical and chemical properties of the ocean. Foraminifera incorporate barium in direct proportion to its concentration in seawater. Using barium as a nutrient proxy, Ba/Ca in benthic Planulina wuellerstorfi is used to reconstruct changes in thermocline ventilation and mid-depth circulation in the North Atlantic during the last glacial and deglacial time. Rivers are concentrated in barium compared to surface seawater. Therefore, barium in planktonic Neogloboquadrina pachyderma is used to identify deglacial meltwater in the Arctic Ocean. Foraminiferal Li/Ca was analyzed to elucidate factors influencing incorporation behavior, including interspecies differences, temperature, pressure, dissolution, and shell mass. To investigate the use of lithium isotopes as a proxy for paleo-seawater chemistry, d6Li was determined in planktonic Orbulina universa. During the last glacial maximum, nutrients in the thermocline and the intermediate water of the North Atlantic was lower than today due to increased ventilation and the presence of nutrient-depleted Glacial North Atlantic Intermediate Water (GNAIW). During deglacial time, GNAIW was replaced by southern component water, resulting in an enrichment of nutrients in the mid-depth Atlantic water. Increased Ba/Ca in the surface Arctic Ocean indicates an increase in meltwater discharge between 12.4 and 11.3 14C ka BP. This may have triggered an increase in the export of freshwater to the North Atlantic, contributing to a shutdown in GNAIW production, and leading to the onset of the Younger Dryas. A second meltwater event at 9.4 14C ka BP may be the result of glacial Lake Agassiz draining through the Clearwater spillway to the Mackenzie River. Foraminiferal Li/Ca shows systematic glacial-interglacial variation coincident with d18O. The incorporation behavior of lithium in foraminifera does not appear to be dominated by changes in temperature, pressure, dissolution, or shell mass, but is potentially controlled by changes in growth conditions, including calcification rate. Preliminary work indicates that d6Li remained constant throughout the Holocene and the last glacial maximum at 30.5 ± 1.1‰. Further developmental studies are necessary to fully engage lithium isotopes as a tracer of seawater composition

Electronic books or print books for increased reading comprehension and vocabulary acquisition in third grade students

Can E-books contribute to increased vocabulary acquisition and reading comprehension in third grade students? Teachers face difficult decisions when determining whether or not to use interactive e-Books in the classroom. Studies (De Jong and Bus 2004; Jones and Brown 2011; Larson, 2009; Verhallen, Bus, and De Jong, 2006), have determined that the multimedia features of e-books have the potential of being beneficial to young children over traditional printed texts; however, studies have failed to prove that these additional text features increase reading achievement over traditional printed text. There has not been enough evidence to show that students reading achievement increases in the general education classroom when they read from interactive e-books rather than traditional printed texts. This study was conducted to determine the differences in third graders\u27 vocabulary acquisition and reading comprehension according to the medium of presentation. Two different story book formats were used, printed copies and electronic versions of the books. Twelve third graders participated in the study. All participants alternated between reading print versions of books and electronic versions using a balanced quasi-experimental design. When students read the electronic versions of the books, they had access to the multimedia features that the e-books provide, whereas the printed versions of the books had the support of the teacher when the students asked for help. After each participant read a book independently they answered ten multiple choice questions and one short answer. All test questions and short answer responses were completed on paper. The results of this study did not provide significant evidence that the additional text features of interactive eBooks increase reading achievement over traditional printed text

Measurement properties of the UK-English version of the Pediatric Quality of Life Inventory™ 4.0 (PedsQL™) generic core scales

Background Health related quality of life (HRQL) has been recognised as an important paediatric outcome measurement. One of the more promising measures to emerge in recent years is the Pediatric Quality Of Life Inventory (PedsQL™), developed in the US. Advantages of the PedsQL™ include brevity, availability of age appropriate versions and parallel forms for child and parent. This study developed a UK-English version of PedsQL™ generic module and assessed its performance in a group of UK children and their parents. Methods PedsQL™ was translated to UK-English. The psychometric properties of the UK version were then tested following administration to 1399 children and 970 of their parents. The sample included healthy children, children diagnosed with asthma, diabetes or inflammatory bowel disease and children in remission from cancer. Results Psychometric properties were similar to those reported for the original PedsQL™. Internal reliability exceeded 0.70 for all proxy and self-report sub-scales. Discriminant validity was established for proxy and self-report with higher HRQL being reported for healthy children than those with health problems. Sex differences were noted on the emotional functioning subscale, with females reporting lower HRQL than males. Proxy and self-report correlation was higher for children with health problems than for healthy children. Conclusion The UK-English version of PedsQL™ performed as well as the original PedsQL™ and is recommended for assessment of paediatric HRQL in the UK

Note and Comment

Internal Revenue Tax on State Dispensaries Upheld; What is the Practice of Medicine?; Appeals from Decrees for Costs; The Hearst Election Contest; The Lapse of a Legacy to a Deceased Child; Unsightly Advertisements and Billboard

Homologous Expression of a Subcomplex of Pyrococcus furiosus Hydrogenase that Interacts with Pyruvate Ferredoxin Oxidoreductase

Hydrogen gas is an attractive alternative fuel as it is carbon neutral and has higher energy content per unit mass than fossil fuels. The biological enzyme responsible for utilizing molecular hydrogen is hydrogenase, a heteromeric metalloenzyme requiring a complex maturation process to assemble its O2-sensitive dinuclear-catalytic site containing nickel and iron atoms. To facilitate their utility in applied processes, it is essential that tools are available to engineer hydrogenases to tailor catalytic activity and electron carrier specificity, and decrease oxygen sensitivity using standard molecular biology techniques. As a model system we are using hydrogen-producing Pyrococcus furiosus, which grows optimally at 100°C. We have taken advantage of a recently developed genetic system that allows markerless chromosomal integrations via homologous recombination. We have combined a new gene marker system with a highly-expressed constitutive promoter to enable high-level homologous expression of an engineered form of the cytoplasmic NADP-dependent hydrogenase (SHI) of P. furiosus. In a step towards obtaining ‘minimal’ hydrogenases, we have successfully produced the heterodimeric form of SHI that contains only two of the four subunits found in the native heterotetrameric enzyme. The heterodimeric form is highly active (150 units mg−1 in H2 production using the artificial electron donor methyl viologen) and thermostable (t1/2 ∼0.5 hour at 90°C). Moreover, the heterodimer does not use NADPH and instead can directly utilize reductant supplied by pyruvate ferredoxin oxidoreductase from P. furiosus. The SHI heterodimer and POR therefore represent a two-enzyme system that oxidizes pyruvate and produces H2 in vitro without the need for an intermediate electron carrier

Tracking key virulence loci encoding aerobactin and salmochelin siderophore synthesis in Klebsiella pneumoniae.

BACKGROUND: Klebsiella pneumoniae is a recognised agent of multidrug-resistant (MDR) healthcare-associated infections; however, individual strains vary in their virulence potential due to the presence of mobile accessory genes. In particular, gene clusters encoding the biosynthesis of siderophores aerobactin (iuc) and salmochelin (iro) are associated with invasive disease and are common amongst hypervirulent K. pneumoniae clones that cause severe community-associated infections such as liver abscess and pneumonia. Concerningly, iuc has also been reported in MDR strains in the hospital setting, where it was associated with increased mortality, highlighting the need to understand, detect and track the mobility of these virulence loci in the K. pneumoniae population. METHODS: Here, we examined the genetic diversity, distribution and mobilisation of iuc and iro loci amongst 2503 K. pneumoniae genomes using comparative genomics approaches and developed tools for tracking them via genomic surveillance. RESULTS: Iro and iuc were detected at low prevalence (< 10%). Considerable genetic diversity was observed, resolving into five iro and six iuc lineages that show distinct patterns of mobilisation and dissemination in the K. pneumoniae population. The major burden of iuc and iro amongst the genomes analysed was due to two linked lineages (iuc1/iro1 74% and iuc2/iro2 14%), each carried by a distinct non-self-transmissible IncFIBK virulence plasmid type that we designate KpVP-1 and KpVP-2. These dominant types also carry hypermucoidy (rmpA) determinants and include all previously described virulence plasmids of K. pneumoniae. The other iuc and iro lineages were associated with diverse plasmids, including some carrying IncFII conjugative transfer regions and some imported from Escherichia coli; the exceptions were iro3 (mobilised by ICEKp1) and iuc4 (fixed in the chromosome of K. pneumoniae subspecies rhinoscleromatis). Iro/iuc mobile genetic elements (MGEs) appear to be stably maintained at high frequency within known hypervirulent strains (ST23, ST86, etc.) but were also detected at low prevalence in others such as MDR strain ST258. CONCLUSIONS: Iuc and iro are mobilised in K. pneumoniae via a limited number of MGEs. This study provides a framework for identifying and tracking these important virulence loci, which will be important for genomic surveillance efforts including monitoring for the emergence of hypervirulent MDR K. pneumoniae strains

Airborne investigations of clear-air turbulence with laser radars

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/33443/1/0000846.pd