CLIPS application user interface for the PC

The majority of applications that utilize expert system development programs for their knowledge representation and inferencing capability require some form of interface with the end user. This interface is more than likely an interaction through the computer screen. When building an application the user interface can prove to be the most difficult and time consuming aspect to program. Commercial products currently exist which address this issue. To keep pace C Language Integrated Production System (CLIPS) will need to find a solution for their lack of an easy to use Application User Interface (AUI). This paper represents a survey of the DoD CLIPS' user community and provides the backbone of a possible solution

Post-ischaemic immunological response in the brain: targeting microglia in ischaemic stroke therapy

Microglia, the major endogenous immune cells of the central nervous system, mediate critical degenerative and regenerative responses in ischaemic stroke. Microglia become "activated", proliferating, and undergoing changes in morphology, gene and protein expression over days and weeks post-ischaemia, with deleterious and beneficial effects. Pro-inflammatory microglia (commonly referred to as M1) exacerbate secondary neuronal injury through the release of reactive oxygen species, cytokines and proteases. In contrast, microglia may facilitate neuronal recovery via tissue and vascular remodelling, through the secretion of anti-inflammatory cytokines and growth factors (a profile often termed M2). This M1/M2 nomenclature does not fully account for the microglial heterogeneity in the ischaemic brain, with some simultaneous expression of both M1 and M2 markers at the single-cell level. Understanding and regulating microglial activation status, reducing detrimental and promoting repair behaviours, present the potential for therapeutic intervention, and open a longer window of opportunity than offered by acute neuroprotective strategies. Pharmacological modulation of microglial activation status to promote anti-inflammatory gene expression can increase neurogenesis and improve functional recovery post-stroke, based on promising preclinical data. Cell-based therapies, using preconditioned microglia, are of interest as a method of therapeutic modulation of the post-ischaemic inflammatory response. Currently, there are no clinically-approved pharmacological options targeting post-ischaemic inflammation. A major developmental challenge for clinical translation will be the selective suppression of the deleterious effects of microglial activity after stroke whilst retaining (or enhancing) the neurovascular repair and remodelling responses of microglia

Long-term modifications of coastal defences enhance marine biodiversity

Realization that hard coastal infrastructures support lower biodiversity than natural habitats has prompted a wealth of research seeking to identify design enhancements offering ecological benefits. Some studies showed that artificial structures could be modified to increase levels of diversity. Most studies, however, only considered the short-term ecological effects of such modifications, even though reliance on results from short-term studies may lead to serious misjudgements in conservation. In this study, a seven-year experiment examined how the addition of small pits to otherwise featureless seawalls may enhance the stocks of a highly-exploited limpet. Modified areas of the seawall supported enhanced stocks of limpets seven years after the addition of pits. Modified areas of the seawall also supported a community that differed in the abundance of littorinids, barnacles and macroalgae compared to the controls. Responses to different treatments (numbers and size of pits) were species-specific and, while some species responded directly to differences among treatments, others might have responded indirectly via changes in the distribution of competing species. This type of habitat enhancement can have positive long-lasting effects on the ecology of urban seascapes. Understanding of species interactions could be used to develop a rule-based approach to enhance biodiversity

Alignment of multiple glial cell populations in 3D nanofiber scaffolds: toward the development of multicellular implantable scaffolds for repair of neural injury

Non-neuronal cells of the central nervous system (CNS), termed "neuroglia," play critical roles in neural regeneration; therefore, replacement of glial populations via implantable nanofabricated devices (providing a growth-permissive niche) is a promising strategy to enhance repair. Most constructs developed to date have lacked three-dimensionality, multiple glial populations and control over spatial orientations, limiting their ability to mimic in vivo neurocytoarchitecture. We describe a facile technique to incorporate multiple glial cell populations [astrocytes, oligodendrocyte precursor cells (OPCs) and oligodendrocytes] within a three-dimensional (3D) nanofabricated construct. Highly aligned nanofibers could induce elongation of astrocytes, while OPC survival, elongation and maturation required pre-aligned astrocytes. The potential to scale-up the numbers of constituent nanofiber layers is demonstrated with astrocytes. Such complex implantable constructs with multiple glial sub-populations in defined 3D orientations could represent an effective approach to reconstruct glial circuitry in neural injury sites

Night-time lighting alters the composition of marine epifaunal communities

Marine benthic communities face multiple anthropogenic pressures that compromise the future of some of the most biodiverse and functionally important ecosystems in the world. Yet one of the pressures these ecosystems face, night-time lighting, remains unstudied. Light is an important cue in guiding the settlement of invertebrate larvae, and altering natural regimes of nocturnal illumination could modify patterns of recruitment among sessile epifauna. We present the first evidence of night-time lighting changing the composition of temperate epifaunal marine invertebrate communities. Illuminating settlement surfaces with white light-emitting diode lighting at night, to levels experienced by these communities locally, both inhibited and encouraged the colonization of 39% of the taxa analysed, including three sessile and two mobile species. Our results indicate that ecological light pollution from coastal development, shipping and offshore infrastructure could be changing the composition of marine epifaunal communities.European Research Council under the European Union's Seventh Framework programme (FP7/2007-2013

A Mouse Amidase Specific for N-terminal Asparagine: the gene, the enzyme, and their function in the N-end rule pathway

The N-end rule relates the in vivo half-life of a protein to the identity of its N-terminal residue. In both fungi and mammals, the tertiary destabilizing N-terminal residues asparagine and glutamine function through their conversion, by enzymatic deamidation, into the secondary destabilizing residues aspartate and glutamate, whose destabilizing activity requires their enzymatic conjugation to arginine, one of the primary destabilizing residues. We report the isolation and analysis of a mouse cDNA and the corresponding gene (termed Ntan1) that encode a 310-residue amidohydrolase (termed NtN-amidase) specific for N-terminal asparagine. The ~17-kilobase pair Ntan1 gene is located in the proximal region of mouse chromosome 16 and contains 10 exons ranging from 54 to 177 base pairs in length. The ~1.4-kilobase pair Ntan1 mRNA is expressed in all of the tested mouse tissues and cell lines and is down-regulated upon the conversion of myoblasts into myotubes. The Ntan1 promoter is located ~500 base pairs upstream of the Ntan1 start codon. The deduced amino acid sequence of mouse NtN-amidase is 88% identical to the sequence of its porcine counterpart, but bears no significant similarity to the sequence of the NTA1-encoded N-terminal amidohydrolase of the yeast Saccharomyces cerevisiae, which can deamidate either N-terminal asparagine or glutamine. The expression of mouse NtN-amidase in S. cerevisiae nta1Delta was used to verify that NtN-amidase retains its asparagine selectivity in vivo and can implement the asparagine-specific subset of the N-end rule. Further dissection of mouse Ntan1, including its null phenotype analysis, should illuminate the functions of the N-end rule, most of which are still unknown

A comparison of spectroscopic methods for detecting starlight scattered by transiting hot Jupiters, with application to Subaru data for HD 209458b and HD 189733b

The measurement of the light scattered from extrasolar planets informs atmospheric and formation models. With the discovery of many hot Jupiter planets orbiting nearby stars, this motivates the development of robust methods of characterisation from follow up observations. In this paper we discuss two methods for determining the planetary albedo in transiting systems. First, the most widely used method for measuring the light scattered by hot Jupiters (Collier Cameron et al.) is investigated for application for typical echelle spectra of a transiting planet system, showing that detection requires high signal-to-noise ratio data of bright planets. Secondly a new Fourier analysis method is also presented, which is model-independent and utilises the benefits of the reduced number of unknown parameters in transiting systems. This approach involves solving for the planet and stellar spectra in Fourier space by least-squares. The sensitivities of the methods are determined via Monte Carlo simulations for a range of planet-to-star fluxes. We find the Fourier analysis method to be better suited to the ideal case of typical observations of a well constrained transiting system than the Collier Cameron et al. method. We apply the Fourier analysis method for extracting the light scattered by transiting hot Jupiters from high resolution spectra to echelle spectra of HD 209458 and HD 189733. Unfortunately we are unable to improve on the previous upper limit of the planet-to-star flux for HD 209458b set by space-based observations. A 1{\sigma}upper limit on the planet-to-star flux of HD 189733b is measured in the wavelength range of 558.83-599.56 nm yielding {\epsilon} < 4.5 \times 10-4. Improvement in the measurement of the upper limit of the planet-to-star flux of this system, with ground-based capabilities, requires data with a higher signal-to-noise ratio, and increased stability of the telescope.Comment: 15 pages, 8 figures, 2 tables. Monthly Notices of the Royal Astronomical Society, in press. Accepted 2011 March 17. Received 2011 March 17; in original form 2010 June 2

Lexical profiles of comprehensible second language speech: the role of appropriateness, fluency, variation, sophistication, abstractness and sense relations

This study examined contributions of lexical factors to native-speaking raters’ assessments of comprehensibility (ease of understanding) of second language (L2) speech. Extemporaneous oral narratives elicited from 40 French speakers of L2 English were transcribed and evaluated for comprehensibility by 10 raters. Subsequently, the samples were analyzed for 12 lexical variables targeting diverse domains of lexical usage (appropriateness, fluency, variation, sophistication, abstractness, and sense relations). For beginner-to-intermediate speakers, comprehensibility was related to basic uses of L2 vocabulary (fluent and accurate use of concrete words). For intermediate-to-advanced speakers, comprehensibility was linked to sophisticated uses of L2 lexis (morphologically accurate use of complex, less familiar, polysemous words). These findings, which highlight complex associations between lexical variables and L2 comprehensibility, suggest that improving comprehensibility requires attention to multiple lexical domains of L2 performance