Regulation of transcription by the Arabidopsis UVR8 photoreceptor involves a specific histone modification

The photoreceptor UV RESISTANCE LOCUS 8 (UVR8) specifically mediates photomorphogenic responses to UV-B wavelengths. UVR8 acts by regulating transcription of a set of genes, but the underlying mechanisms are unknown. Previous research indicated that UVR8 can associate with chromatin, but the specificity and functional significance of this interaction are not clear. Here we show, by chromatin immunoprecipitation, that UV-B exposure of Arabidopsis increases acetylation of lysines K9 and/or K14 of histone H3 at UVR8-regulated gene loci in a UVR8-dependent manner. The transcription factors HY5 and/or HYH, which mediate UVR8-regulated transcription, are also required for this chromatin modification, at least for the ELIP1 gene. Furthermore, sequencing of the immunoprecipitated DNA revealed that all UV-B-induced enrichments in H3K9,14diacetylation across the genome are UVR8-dependent, and approximately 40 % of the enriched loci contain known UVR8-regulated genes. In addition, inhibition of histone acetylation by anacardic acid reduces the UV-B induced, UVR8 mediated expression of ELIP1 and CHS. No evidence was obtained in yeast 2-hybrid assays for a direct interaction between either UVR8 or HY5 and several proteins involved in light-regulated histone modification, nor for the involvement of these proteins in UVR8-mediated responses in plants, although functional redundancy between proteins could influence the results. In summary, this study shows that UVR8 regulates a specific chromatin modification associated with transcriptional regulation of a set of UVR8-target genes

Converting energy from fusion into useful forms

If fusion power reactors are to be feasible, it will still be necessary to convert the energy of the nuclear reaction into usable form. The heat produced will be removed from the reactor core by a primary coolant, which might be water, helium, molten lithium-lead, molten lithium-containing salt, or CO2. The heat could then be transferred to a conventional Rankine cycle or Brayton (gas turbine) cycle. Alternatively it could be used for thermochemical processes such as producing hydrogen or other transport fuels. Fusion presents new problems because of the high energy neutrons released. These affect the selection of materials and the operating temperature, ultimately determining the choice of coolant and working cycle. The limited temperature ranges allowed by present day irradiated structural materials, combined with the large internal power demand of the plant, will limit the overall thermal efficiency. The operating conditions of the fusion power source, the materials, coolant, and energy conversion system will all need to be closely integrated.Comment: 22 pages, 4 figures, Proceedings of the Institution of Mechanical Engineers, Part A: Journal of Power and Energy December 11, 201

Regulation of UVR8 photoreceptor dimer/monomer photo-equilibrium in Arabidopsis plants grown under photoperiodic conditions

The UV RESISTANCE LOCUS 8 (UVR8) photoreceptor specifically mediates photomorphogenic responses to UV-B. Photoreception induces dissociation of dimeric UVR8 into monomers to initiate responses. However, the regulation of dimer/monomer status in plants growing under photoperiodic conditions has not been examined. Here we show that UVR8 establishes a dimer/monomer photo-equilibrium in plants growing in diurnal photoperiods in both controlled environments and natural daylight. The photo-equilibrium is determined by the relative rates of photoreception and dark-reversion to the dimer. Experiments with mutants in REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 (RUP1) and RUP2 show that these proteins are crucial in regulating the photo-equilibrium because they promote reversion to the dimer. In plants growing in daylight, the UVR8 photo-equilibrium is most strongly correlated with low ambient fluence rates of UV-B (up to 1.5 µmol m−2 s−1), rather than higher fluence rates or the amount of photosynthetically active radiation. In addition, the rate of reversion of monomer to dimer is reduced at lower temperatures, promoting an increase in the relative level of monomer at approximately 8–10 °C. Thus, UVR8 does not behave like a simple UV-B switch under photoperiodic growth conditions but establishes a dimer/monomer photo-equilibrium that is regulated by UV-B and also influenced by temperature

On Putting Knowledge 'First'

There is a New Idea in epistemology. It goes by the name of ‘knowledge first,’ and it is particularly associated with Timothy Williamson’s book Knowledge and Its Limits. In slogan form, to put knowledge first is to treat knowledge as basic or fundamental, and to explain other states—belief, justification, maybe even content itself—in terms of knowledge, instead of vice versa. The idea has proven enormously interesting, and equally controversial. But deep foundational questions about its actual content remain relatively unexplored. We think that a wide variety of views travel under the banner of ‘knowledge first’ (and that the slogan doesn’t help much with differentiating them). Furthermore, we think it is far from straightforward to draw connections between certain of these views; they are more independent than they are often assumed to be. Our project here is exploratory and clarificatory. We mean to tease apart various ‘knowledge first’ claims, and explore what connections they do or do not have with one another. Our taxonomy is offered in §2, and connections are explored in §3. The result, we hope, will be a clearer understanding of just what the knowledge first theses are. We conclude, in §4, with some brief suggestions as to how we think the various theses might be evaluated

Giant planets around two intermediate-mass evolved stars and confirmation of the planetary nature of HIP67851 c

Precision radial velocities are required to discover and characterize planets orbiting nearby stars. Optical and near infrared spectra that exhibit many hundreds of absorption lines can allow the m/s precision levels required for such work. However, this means that studies have generally focused on solar-type dwarf stars. After the main-sequence, intermediate-mass stars (former A-F stars) expand and rotate slower than their progenitors, thus thousands of narrow absorption lines appear in the optical region, permitting the search for planetary Doppler signals in the data for these types of stars. We present the discovery of two giant planets around the intermediate-mass evolved star HIP65891 and HIP107773. The best Keplerian fit to the HIP65891 and HIP107773 radial velocities leads to the following orbital parameters: P=1084.5 d; m$_b$sin$i$ = 6.0 M$_{jup}$; $e$=0.13 and P=144.3 d; m$_b$sin$i$ = 2.0 M$_{jup}$; $e$=0.09, respectively. In addition, we confirm the planetary nature of the outer object orbiting the giant star HIP67851. The orbital parameters of HIP67851c are: P=2131.8 d, m$_c$sin$i$ = 6.0 M$_{jup}$ and $e$=0.17. With masses of 2.5 M$_\odot$ and 2.4 M$_\odot$ HIP65891 and HIP107773 are two of the most massive stars known to host planets. Additionally, HIP67851 is one of five giant stars that are known to host a planetary system having a close-in planet ($a <$ 0.7 AU). Based on the evolutionary states of those five stars, we conclude that close-in planets do exist in multiple systems around subgiants and slightly evolved giants stars, but probably they are subsequently destroyed by the stellar envelope during the ascent of the red giant branch phase. As a consequence, planetary systems with close-in objects are not found around horizontal branch stars.Comment: Accepted for publication in A&

Critical Analysis of Baryon Masses and Sigma-Terms in Heavy Baryon Chiral Perturbation Theory

We present an analysis of the octet baryon masses and the $\pi N$ and $KN$ $\sigma$--terms in the framework of heavy baryon chiral perturbation theory. At next-to-leading order, ${\cal O}(q^3)$, knowledge of the baryon masses and $\sigma_{\pi N}(0)$ allows to determine the three corresponding finite low--energy constants and to predict the the two $KN$ $\sigma$--terms $\sigma^{(1,2)}_{KN} (0)$. We also include the spin-3/2 decuplet in the effective theory. The presence of the non--vanishing energy scale due to the octet--decuplet splitting shifts the average octet baryon mass by an infinite amount and leads to infinite renormalizations of the low--energy constants. The first observable effect of the decuplet intermediate states to the baryon masses starts out at order $q^4$. We argue that it is not sufficient to retain only these but no other higher order terms to achieve a consistent description of the three--flavor scalar sector of baryon CHPT. In addition, we critically discuss an SU(2) result which allows to explain the large shift of $\sigma_{\pi N}(2M_\pi^2) - \sigma_{\pi N}(0)$ via intermediate $\Delta (1232)$ states.Comment: 18 pp, TeX, BUTP-93/05 and CRN-93-0

Electromagnetic Moments of the Baryon Decuplet

We compute the leading contributions to the magnetic dipole and electric quadrupole moments of the baryon decuplet in chiral perturbation theory. The measured value for the magnetic moment of the $\Omega^-$ is used to determine the local counterterm for the magnetic moments. We compare the chiral perturbation theory predictions for the magnetic moments of the decuplet with those of the baryon octet and find reasonable agreement with the predictions of the large--$N_c$ limit of QCD. The leading contribution to the quadrupole moment of the $\Delta$ and other members of the decuplet comes from one--loop graphs. The pionic contribution is shown to be proportional to $I_z$ (and so will not contribute to the quadrupole moment of $I=0$ nuclei), while the contribution from kaons has both isovector and isoscalar components. The chiral logarithmic enhancement of both pion and kaon loops has a coefficient that vanishes in the $SU(6)$ limit. The third allowed moment, the magnetic octupole, is shown to be dominated by a local counterterm with corrections arising at two loops. We briefly mention the strange counterparts of these moments.Comment: Uses harvmac.tex, 15 pages with 3 PostScript figures packed using uufiles. UCSD/PTH 93-22, QUSTH-93-05, Duke-TH-93-5

A Survey of Lepton Number Violation Via Effective Operators

We survey 129 lepton number violating effective operators, consistent with the minimal Standard Model gauge group and particle content, of mass dimension up to and including eleven. Upon requiring that each one radiatively generates the observed neutrino masses, we extract an associated characteristic cutoff energy scale which we use to calculate other observable manifestations of these operators for a number of current and future experimental probes, concentrating on lepton number violating phenomena. These include searches for neutrinoless double-beta decay and rare meson, lepton, and gauge boson decays. We also consider searches at hadron/lepton collider facilities in anticipation of the LHC and the future ILC. We find that some operators are already disfavored by current data, while more are ripe to be probed by next-generation experiments. We also find that our current understanding of lepton mixing disfavors a subset of higher dimensional operators. While neutrinoless double-beta decay is the most promising signature of lepton number violation for the majority of operators, a handful is best probed by other means. We argue that a combination of constraints from various independent experimental sources will help to pinpoint the ``correct'' model of neutrino mass, or at least aid in narrowing down the set of possibilities.Comment: 34 pages, 10 eps figures, 1 tabl

We Could, but Should We? Ethical Considerations for Providing Access to GeoCities and Other Historical Digital Collections

We live in an era in which the ways that we can make sense of our past are evolving as more artifacts from that past become digital. At the same time, the responsibilities of traditional gatekeepers who have negotiated the ethics of historical data collection and use, such as librarians and archivists, are increasingly being sidelined by the system builders who decide whether and how to provide access to historical digital collections, often without sufficient reflection on the ethical issues at hand. It is our aim to better prepare system builders to grapple with these issues. This paper focuses discussions around one such digital collection from the dawn of the web, asking what sorts of analyses can and should be conducted on archival copies of the GeoCities web hosting platform that dates to 1994.This research was supported by the Natural Sciences and Engineering Research Council of Canada, the Social Sciences and Humanities Research Council of Canada, the US National Science Foundation (grants 1618695 and 1704369), the Andrew W. Mellon Foundation, Start Smart Labs, and Compute Canada