Effect of group size and chlortetracycline on Salmonella in swine

The objective of this study was to evaluate the effect of stocking density and subtherapeutic chlortetracycline (CTC) on Salmonella prevalence in swine

Occurrence of the invasion associated marker (iam) in Campylobacter jejuni isolated from cattle

Background The invasion associated marker (iam) has been detected in the majority of invasive Campylobacter jejuni retrieved from humans. Furthermore, the detection of iam in C. jejuni isolated from two important hosts, humans and chickens, suggested a role for this marker in C. jejuni\u27s colonization of multiple hosts. However, no data exist regarding the occurrence of this marker in C. jejuni isolated from non-poultry food-animals such as cattle, an increasingly important source for human infections. Since little is known about the genetics associated with C. jejuni\u27s capability for colonizing physiologically disparate hosts, we investigated the occurrence of the iam in C. jejuni isolated from cattle and assessed the potential of iam-containing cattle and human isolates for chicken colonization and human cell invasion. Results Simultaneous RAPD typing and iam-specific PCR analysis of 129 C. jejuni isolated from 1171 cattle fecal samples showed that 8 (6.2%) of the isolates were iam-positive, while 7 (54%) of human-associated isolates were iam-positive. The iam sequences were mostly heterogeneous and occurred in diverse genetic backgrounds. All iam-positive isolates were motile and possessed important genes (cadF, ciaB, cdtB) associated with adhesion and virulence. Although certain iam-containing isolates invaded and survived in INT-407 cells in high numbers and successfully colonized live chickens, there was no clear association between the occurrence, allelic sequence, and expression levels of the iam and the aforementioned phenotypes. Conclusions We show that the prevalence of iam in cattle C. jejuni is relatively lower as compared to isolates occurring in humans and chickens. In addition, iam was polymorphic and certain alleles occur in cattle isolates that were capable of colonizing and invading chickens and human intestinal cells, respectively. However, the iam did not appear to contribute to the cattle-associated C. jejuni\u27s potential for invasion and intracellular survival in human intestinal cells as well as chicken colonization

Which effective viscosity?

Magmas undergoing shear are prime examples of flows that involve the transport of solids and gases by a separate (silicate melt) carrier phase. Such flows are called multiphase, and have attracted much attention due to their important range of engineering applications. Where the volume fraction of the dispersed phase (crystals) is large, the influence of particles on the fluid motion becomes significant and must be taken into account in any explanation of the bulk behaviour of the mixture. For congested magma deforming well in excess of the dilute limit (particle concentrations >40% by volume), sudden changes in the effective or relative viscosity can be expected. The picture is complicated further by the fact that the melt phase is temperature- and shear-rate-dependent. In the absence of a constitutive law for the flow of congested magma under an applied force, it is far from clear which of the many hundreds of empirical formulae devised to predict the rheology of suspensions as the particle fraction increases with time are best suited. Some of the more commonly used expressions in geology and engineering are reviewed with an aim to home in on those variables key to an improved understanding of magma rheology. These include a temperature, compositional and shear-rate dependency of viscosity of the melt phase with the shear-rate dependency of the crystal (particle) packing arrangement. Building on previous formulations, a new expression for the effective (relative) viscosity of magma is proposed that gives users the option to define a packing fraction range as a function of shear stress. Comparison is drawn between processes (segregation, clustering, jamming), common in industrial slurries, and structures seen preserved in igneous rocks. An equivalence is made such that congested magma, viewed in purely mechanical terms as a high-temperature slurry, is an inherently non-equilibrium material where flow at large Péclet numbers may result in shear thinning and spontaneous development of layering

Comments on "Viscosity of high crystal content melts: dependence on solid fraction"

Here we propose a simple modification of the parameterisation presented in Costa (2005) [Viscosity of high crystal content melts: dependence on solid fraction, Geophys. Res. Lett., 32, 2005] which improves all the main positive features of it, yet at the same time, does not show a plateau region as the solid fraction tends to 1, generally increases the performance of the model, and exactly recovers the classical well established relationships valid for small fractions.Comment: 6 pages, 2 figure, comments on a previous paper published on Geophys. Res. Let

Human Escherichia coli O157:H7 Genetic Marker in Isolates of Bovine Origin

The antiterminator Q gene of bacteriophage 933W (Q933) was identified upstream of the stx2 gene in 90% of human disease–origin Escherichia coli O157:H7 isolates and in 44.5% of bovine isolates. Shiga toxin production was higher in Q933-positive isolates than Q933-negative isolates. This genetic marker may provide a useful molecular tool for epidemiologic studies

Multiwavelength Study of the Starburst Galaxy NGC 7714. II: The Balance between Young, Intermediate Age and Old Stars

We combine existing multiwavelength data (incl. an HST/GHRS UV spectrum, an optical spectrum, far-IR, Xray and radio fluxes) with new HST/WFPC2 images, near-IR photometry and K band spectroscopy. We use these data to constrain the young, the intermediate age and the old stellar populations in the central 330 pc of the starburst galaxy NGC 7714. [...] We find that the young burst responsible for the UV light is only a small part of an extended episode of enhanced star formation (SF) [...]. The mass of young and intermediate age stars thus formed equals at least 10% of the mass locked in pre-existing stars of the underlying galaxy nucleus [...]. The spectrophotometric SF timescale is long compared to the ~110 Myr elapsed since closest contact with NGC 7715. The trigger of the starburst remains elusive. NGC 7714 owes its brightness in the UV to a few low extinction lines of sight towards young stars. [...] The different extinction estimates obtained from different indicators result naturally from the coexistence of populations with various ages and obscurations. The near-IR continuum image looks smoothest, as a consequence of lower sensitivity to extinction and of a larger contribution of old stars. We compare the nuclear properties of NGC 7714 with results from studies in larger apertures. We emphasize that the global properties of starburst galaxies are the result of the averaging over many lines of sight with diverse properties in terms of obscuration and stellar ages.Comment: 29 pages (+20 figures and tables), Latex2e (figs. included), uses aastex.cls. To be published in ApJ (May 2001 issue

Global Distribution of Outbreaks of Water-Associated Infectious Diseases

Water is essential for maintaining life on Earth but can also serve as a media for many pathogenic organisms, causing a high disease burden globally. However, how the global distribution of water-associated infectious pathogens/diseases looks like and how such distribution is related to possible social and environmental factors remain largely unknown. In this study, we compiled a database on distribution, biology, and epidemiology of water-associated infectious diseases and collected data on population density, annual accumulated temperature, surface water areas, average annual precipitation, and per capita GDP at the global scale. From the database we extracted reported outbreak events from 1991 to 2008 and developed models to explore the association between the distribution of these outbreaks and social and environmental factors. A total of1,428 outbreaks had been reported and this number only reflected ‘the tip of the iceberg’ of the much bigger problem. We found that the outbreaks of water-associated infectious diseases are significantly correlated with social and environmental factors and that all regions are affected disproportionately by different categories of diseases. Relative risk maps are generated to show ‘hotspots’ of risks for different diseases. Despite certain limitations, the findings may be instrumental for future studies and prioritizing health resources

Quality indicators for patients with traumatic brain injury in European intensive care units

Background: The aim of this study is to validate a previously published consensus-based quality indicator set for the management of patients with traumatic brain injury (TBI) at intensive care units (ICUs) in Europe and to study its potential for quality measur