Early maturation and substance use across adolescence and young adulthood : A longitudinal study of Finnish twins

Early maturation, indexed by pubertal development (PD), has been associated with earlier initiation and greater frequency of adolescent substance use, but this relationship may be biased by confounding factors and effects that change across development. Using a population-based Finnish twin sample (N = 3,632 individuals), we conducted twin modeling and multilevel structural equation modeling of the relationship between PD and substance use at ages 12-22. Shared environmental factors contributed to early PD and heavier substance use for females. Biological father absence was associated with early PD for boys but not girls, and did not account for the relationship between PD and substance use. The association between early PD and heavier substance use was partially due to between-family confounds, although early PD appeared to qualitatively alter long-term trajectories for some substances (nicotine), but not others (alcohol). Mediation by peer and parental factors did not explain this relationship within families. However, higher peer substance use and lower parental monitoring were themselves associated with heavier substance use, strengthening the existing evidence for these factors as targets for prevention/intervention efforts. Early maturation was not supported as a robust determinant of alcohol use trajectories in adolescence and young adulthood, but may require longer term follow-up. Subtle effects of early PD on nicotine and illicit drug use trajectories throughout adolescence and adulthood merit further investigation.Peer reviewe

Schistosoma mansoni : use of a fluorescent indicator to detect nitric oxide and related species in living parasites

Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Experimental Parasitology 113 (2006): 130-133, doi:10.1016/j.exppara.2005.12.013.Nitric oxide (NO) is synthesized enzymatically by nitric oxide synthase (NOS). Several groups have previously presented evidence for NOS activity and immunoreactivity in several parasitic platyhelminths, including schistosomes. Here, we use 4,5-diaminofluorescein-2 diacetate (DAF-2 DA), a fluorescent indicator of NO, to detect NO in living schistosomes. In adult worms, DAF-2 fluorescence is found selectively in epithelial-like cells. Fluorescence increases when worms are incubated in L-arginine, the precursor of NO synthesis, and decreases dramatically in the presence of the NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME), indicating that predicted NO release may be NOS-dependent, and that enzymatic NO signaling pathways may play an important role in schistosome physiology.This work was supported by NIH grant NS 39103 and NSF grants 0304569 (LLM), and NIH grant AI 40522 and the Neal Cornell Research Fund at the Marine Biological Laboratory (RMG)

RiskBenefit4EU project – a strategy for risk-benefit assessment of foods in Portugal

O balanço entre os riscos e benefícios para a saúde resultante do consumo de alimentos é um importante contributo para apoiar a definição de políticas de saúde e a promoção da literacia dos consumidores. No âmbito do projeto RiskBenefit4EU, financiado pela European Food Safety Authority e coordenado pelo Departamento de Alimentação e Nutrição do Instituto Nacional de Saúde Doutor Ricardo Jorge, o presente trabalho pretende descrever a estratégia implementada para a capacitação das equipas portuguesas em avaliação de risco-benefício de alimentos (RBA). Concretizada pelos parceiros do Institut National de la Recherche Agronomique e da Technical University of Denmark, a capacitação consiste em três atividades principais: 1) Formação teórica, focando os conceitos-chave para a avaliação de RBA; 2) Formação prática, aplicando os conceitos adquiridos e as metodologias transmitidas a um estudo de caso; e 3) Missões científicas, de curta duração, para formação avançada em domínios específicos da avaliação de RBA. No que diz respeito à formação prática, e com o objetivo de consolidar os conhecimentos adquiridos em avaliação de RBA, está previsto o desenvolvimento de um estudo de caso português sobre alimentos à base de cereais habitualmente consumidos por crianças. A estratégia de capacitação seguida neste projeto servirá de modelo para outras equipas e países, contribuindo para a disseminação de uma cultura de avaliação de RBA nas vertentes toxicológica, microbiológica e nutricional a nível internacional.The balance of risks and health benefits from food consumption constitutes a crucial topic to consumer literacy and health policy-makers. Through the RiskBenefit4EU project, funded by the European Food Safety Authority, and coordinated by the Food and Nutrition Department of Portuguese National Institute of Health Dr. Ricardo Jorge, the present work intends to identify the applied strategy to capacitate the Portuguese teams for the development and implementation of risk-benefit assessment (RBA) in food. The training of the Portuguese team is being accomplished by the French National Institute for Agricultural Research and the Technical University of Denmark members, through three main capacity building activities: 1) Theoretical training, focusing on the key concepts for RBA; 2) Practical training, applying the concepts acquired and the methodologies transmitted to a concrete case study; and 3) Short-term scientific missions for advanced training in specific areas of RBA. In order to complete the training of the Portuguese teams and consolidate the knowledge acquired in RBA, a Portuguese case study on cereal-based foods usually consumed by children is planned. The training strategy followed in this project will contribute as a model of capacity building for disseminating a culture of risk-benefit assessment in the toxicological, microbiological and nutritional aspects at the international level.Trabalho desenvolvido no âmbito do projeto “RiskBenefit4EU – Partnering to strengthen the risk-benefit assessment within EU using a holistic approach” financiado pela EFSA Partnering Grants (Grant Agreement Number A/EFSA/AFSCO/2017/01 – GA02).info:eu-repo/semantics/publishedVersio

Adolescent to young adult longitudinal development of subcortical volumes in two European sites with four waves

Adolescent subcortical structural brain development might underlie psychopathological symptoms, which often emerge in adolescence. At the same time, sex differences exist in psychopathology, which might be mirrored in underlying sex differences in structural development. However, previous studies showed inconsistencies in subcortical trajectories and potential sex differences. Therefore, we aimed to investigate the subcortical structural trajectories and their sex differences across adolescence using for the first time a single cohort design, the same quality control procedure, software, and a general additive mixed modeling approach. We investigated two large European sites from ages 14 to 24 with 503 participants and 1408 total scans from France and Germany as part of the IMAGEN project including four waves of data acquisition. We found significantly larger volumes in males versus females in both sites and across all seven subcortical regions. Sex differences in age-related trajectories were observed across all regions in both sites. Our findings provide further evidence of sex differences in longitudinal adolescent brain development of subcortical regions and thus might eventually support the relationship of underlying brain development and different adolescent psychopathology in boys and girls.</p

Consumption of cereal-based foods by Portuguese children: a risk-benefit assessment

De uma forma geral, o consumo de alimentos pode apresentar potenciais riscos e benefícios para os consumidores. Os alimentos à base de cereais, incluindo os cereais de pequeno-almoço e os cereais infantis, representam componentes importantes da dieta humana e estão entre os primeiros alimentos sólidos que são introduzidos na dieta. Estes alimentos constituem uma fonte importante de vários nutrientes, incluindo o sódio, fibras e açúcares livres, que podem estar associados a efeitos benéficos e adversos para a saúde. Simultaneamente, os alimentos à base de cereais podem também ser veículo de contaminantes químicos (por exemplo, micotoxinas) e microbiológicos (por exemplo, Bacillus cereus). As aflatoxinas, um grupo de micotoxinas que apresenta maior potencial tóxico, são frequentemente detetadas em cereais. No âmbito do projeto RiskBenefit4EU, o presente estudo teve como objetivo avaliar os riscos e os benefícios associados ao consumo de alimentos à base de cereais pelas crianças portuguesas. Os riscos (teores de aflatoxinas e B. cereus, sódio e açúcares livres) e os benefícios (teor de fibra) associados ao consumo de cereais de pequeno-almoço (CPA) foram comparados com aqueles associados ao consumo de cereais infantis (CI). Os resultados obtidos revelaram que a mudança do consumo atual para os cenários alternativos considerados (em especial para o consumo de cereais de pequeno-almoço com características semelhantes ao “Melhor CPA”) poderia resultar num ganho de anos de vida saudável.Foodstuffs, as cereal-based products, may present both potential risks and benefits to consumers. Cereal-based foods including breakfast and infant cereals represent important components of human diets and are among the first solid foods that young children usually eat. Cereal-based products constitute a source of several nutrients, including sodium, fibre and free sugars, which could be associated to beneficial and hazardous effects. Simultaneously, cereal-based foods could also present chemical (e.g. mycotoxins) and microbiological (e.g. Bacillus cereus) contaminants. Aflatoxins, a frequent mycotoxin found in cereals, are undoubtedly the most toxic. Developed under RiskBenefit4EU project, the present study aimed to assess risks and benefits associated with the consumption of cereal-based products by the Portuguese young children. The risks posed by the consumption of breakfast cereals (BC) versus infant cereals (IC), due to aflatoxins, B. cereus, sodium and free sugars, were evaluated against the benefits of the intake of fibre. Obtained results showed that moving from the current consumption to the considered alternative scenarios (especially if considered the consumption of breakfast cereals similar to “Best BC”) could result in a gain of healthy-life years.Este trabalho foi desenvolvido no âmbito do projeto RiskBenefit4EU – Partnering to strengthen the risk-benefit assessment within EU using a holistic approach financiado pelas EFSA Partnering Grants (Grant Agreement Number – GA/EFSA/AFSCO/2017/01 – GA02) 1.info:eu-repo/semantics/publishedVersio

Decreased mitochondrial respiration in aneurysmal aortas of Fibulin-4 mutant mice is linked to PGC1A regulation

Aim Thoracic aortic aneurysms are a life-threatening condition often diagnosed too late. To discover novel robust biomarkers, we aimed to better understand the molecular mechanisms underlying aneurysm formation. Methods and results In Fibulin-4R/R mice, the extracellular matrix protein Fibulin-4 is 4-fold reduced, resulting in progressive ascending aneurysm formation and early death around 3 months of age. We performed proteomics and genomics studies on Fibulin-4R/R mouse aortas. Intriguingly, we observed alterations in mitochondrial protein composition in Fibulin-4R/R aortas. Consistently, functional studies in Fibulin-4R/R vascular smooth muscle cells (VSMCs) revealed lower oxygen consumption rates, but increased acidification rates. Yet, mitochondria in Fibulin-4R/R VSMCs showed no aberrant cytoplasmic localization. We found similar reduced mitochondrial respiration in Tgfbr-1M318R/+ VSMCs, a mouse model for Loeys-Dietz syndrome (LDS). Interestingly, also human fibroblasts from Marfan (FBN1) and LDS (TGFBR2 and SMAD3) patients showed lower oxygen consumption. While individual mitochondrial Complexes I–V activities were unaltered in Fibulin-4R/R heart and muscle, these tissues showed similar decreased oxygen consumption. Furthermore, aortas of aneurysmal Fibulin-4R/R mice displayed increased reactive oxygen species (ROS) levels. Consistent with these findings, gene expression analyses revealed dysregulation of metabolic pathways. Accordingly, blood ketone levels of Fibulin-4R/R mice were reduced and liver fatty acids were decreased, while liver glycogen was increased, indicating dysregulated metabolism at the organismal level. As predicted by gene expression analysis, the activity of PGC1α, a key regulator between mitochondrial function and organismal metabolism, was downregulated in Fibulin-4R/R VSMCs. Increased TGFβ reduced PGC1α levels, indicating involvement of TGFβ signalling in PGC1α regulation. Activation of PGC1α restored the decreased oxygen consumption in Fibulin-4R/R VSMCs and improved their reduced growth potential, emphasizing the importance of this key regulator. Conclusion Our data indicate altered mitochondrial function and metabolic dysregulation, leading to increased ROS levels and altered energy production, as a novel mechanism, which may contribute to thoracic aortic aneurysm formation

Spectrum of mutational signatures in T-cell lymphoma reveals a key role for UV radiation in cutaneous T-cell lymphoma

Funder: Galderma; doi: http://dx.doi.org/10.13039/501100009754Funder: NIHR-BRC Cambridge core grantFunder: National Institute for Health Research; doi: http://dx.doi.org/10.13039/501100000272Funder: NHS EnglandAbstract: T-cell non-Hodgkin’s lymphomas develop following transformation of tissue resident T-cells. We performed a meta-analysis of whole exome sequencing data from 403 patients with eight subtypes of T-cell non-Hodgkin’s lymphoma to identify mutational signatures and associated recurrent gene mutations. Signature 1, indicative of age-related deamination, was prevalent across all T-cell lymphomas, reflecting the derivation of these malignancies from memory T-cells. Adult T-cell leukemia-lymphoma was specifically associated with signature 17, which was found to correlate with the IRF4 K59R mutation that is exclusive to Adult T-cell leukemia-lymphoma. Signature 7, implicating UV exposure was uniquely identified in cutaneous T-cell lymphoma (CTCL), contributing 52% of the mutational burden in mycosis fungoides and 23% in Sezary syndrome. Importantly this UV signature was observed in CD4 + T-cells isolated from the blood of Sezary syndrome patients suggesting extensive re-circulation of these T-cells through skin and blood. Analysis of non-Hodgkin’s T-cell lymphoma cases submitted to the national 100,000 WGS project confirmed that signature 7 was only identified in CTCL strongly implicating UV radiation in the pathogenesis of cutaneous T-cell lymphoma

Spray drying of pomegranate juice using maltodextrin/cyclodextrin blends as the wall material

Microencapsulation protects sensitive nutrients, masks flavors, or enhances delivery. Ratios of maltodextrin and γ‐cyclodextrin (20:0, 19:1, and 17:3% w/w) were dissolved in water and mixed with pomegranate juice for spray drying with inlet temperatures of 120, 140, and 160°C. The effects on physical properties (water activity, % water content, color, pH, soluble solids (Brix), and methyl cellulose precipitable tannin assay (MCPTA) were examined. Based on the principle component analysis, formulation influenced color parameters and pH accounted for 46.8% of the variation in the data. Temperature influenced Chroma and water‐holding capacity with 31.8% of the variation. The pH of the reconstituted spray‐dried powder significantly influenced color. Blending of γ‐cyclodextrins to maltodextrins slightly increased the water‐holding capacity, increased pH, slightly affected color, and preserved the color over time, slightly better. Increased inlet temperature affected color, decreased water‐holding capacity, and decreased astringency index. Small additions of γ‐cyclodextrin affect spray‐dried powders

Properties of bone from Catfish heads and frames

The objective of this paper was to evaluate methods of producing purified Catfish bone fractions from Catfish frames and heads and determine the composition of the purified bone fraction. Fresh samples of Catfish frames and heads were obtained from a large commercial Catfish processor. Triplicate samples were processed for all treatments. Two methods were developed to remove nonbone tissue from the frames: (a) use of a proteolytic enzyme to digest the nonbone tissues and (b) after boiling the frames, removal of the nonbone tissues with high‐pressure water. The ash, protein, and lipid contents of unprocessed dried frames were 17%, 33%, and 41%, respectively. After the enzymatic or high‐pressure water treatment processes, the frame bone compositions for the two processes were 62% and 54% ash, 35% and 33% protein, and 9% and 2% lipid, respectively. Bone from both processing treatments had a calcium content of 21%–25%, phosphorus content of 10%–11%, and contents of magnesium, manganese, zinc, and nickel were increased. Hydroxyproline content increased from 4% of the amino acids in the untreated bone to 7%–8% for the processed treatments. Tissues were removed from Catfish heads by digestion with a proteolytic enzyme and collection of the bone with a sieve. After the longest digestion period, dried head bone was 51% ash, 38% protein, and 7% lipid. The amino acid profiles showed high levels of hydroxyproline and lower levels of many essential amino acids. With increased enzymatic hydrolysis time, percent calcium and phosphorus increased. Results from this study will be used in the development of new value‐added food and feed ingredients from Catfish bone