Robust Output Feedback Disturbance Rejection Control by Simultaneously Estimating State and Disturbance

This paper tackles the problem of simultaneous estimation of the state and the unknown disturbance of an MIMO disturbed system and designs the disturbance rejection controller according to the estimation information. Through a series of transformations, we can transform the original system into two subsystems and then propose a sliding mode observer and a descriptor system form observer, respectively. Our algorithm can simultaneously estimate the state and the unknown disturbance. The estimation error is shown to be bounded within a small region. Moreover, the controller algorithm developed in this paper can effectively avoid the peaking phenomenon. Finally, the feasibility and the performance using the proposed method are analyzed and demonstrated with two simulated examples

On Chattering-Free Dynamic Sliding Mode Controller Design

For a class of linear MIMO uncertain systems, a dynamic sliding mode control algorithm that avoids the chattering problem is proposed in this paper. Without using any differentiator, we develop a modified asymptotically stable second-order sliding mode control law in which the proposed controller can guarantee the finite time convergence to the sliding mode and can show that the system states asymptotically approach to zero. Finally, a numerical example is explained for demonstrating the applicability of the proposed scheme

Robust Output Feedback Disturbance Rejection Control by Simultaneously Estimating State and Disturbance

This paper tackles the problem of simultaneous estimation of the state and the unknown disturbance of an MIMO disturbed system and designs the disturbance rejection controller according to the estimation information. Through a series of transformations, we can transform the original system into two subsystems and then propose a sliding mode observer and a descriptor system form observer, respectively. Our algorithm can simultaneously estimate the state and the unknown disturbance. The estimation error is shown to be bounded within a small region. Moreover, the controller algorithm developed in this paper can effectively avoid the peaking phenomenon. Finally, the feasibility and the performance using the proposed method are analyzed and demonstrated with two simulated examples

Output Feedback Variable Structure Control Design for Uncertain Nonlinear Lipschitz Systems

This paper develops a full-order compensator-based output feedback variable structure control law for uncertain nonlinear Lipschitz systems having matched perturbations. Given that the sufficient condition is satisfied, the developed control scheme, with the observer-like technique incorporated into the design of the compensator, can achieve global exponential stabilization. An illustrative example is provided with simulation results to show the effectiveness of the proposed method

Epstein–Barr Virus DNase (BGLF5) induces genomic instability in human epithelial cells

Epstein–Barr Virus (EBV) DNase (BGLF5) is an alkaline nuclease and has been suggested to be important in the viral life cycle. However, its effect on host cells remains unknown. Serological and histopathological studies implied that EBV DNase seems to be correlated with carcinogenesis. Therefore, we investigate the effect of EBV DNase on epithelial cells. Here, we report that expression of EBV DNase induces increased formation of micronucleus, an indicator of genomic instability, in human epithelial cells. We also demonstrate, using γH2AX formation and comet assay, that EBV DNase induces DNA damage. Furthermore, using host cell reactivation assay, we find that EBV DNase expression repressed damaged DNA repair in various epithelial cells. Western blot and quantitative PCR analyses reveal that expression of repair-related genes is reduced significantly in cells expressing EBV DNase. Host shut-off mutants eliminate shut-off expression of repair genes and repress damaged DNA repair, suggesting that shut-off function of BGLF5 contributes to repression of DNA repair. In addition, EBV DNase caused chromosomal aberrations and increased the microsatellite instability (MSI) and frequency of genetic mutation in human epithelial cells. Together, we propose that EBV DNase induces genomic instability in epithelial cells, which may be through induction of DNA damage and also repression of DNA repair, subsequently increases MSI and genetic mutations, and may contribute consequently to the carcinogenesis of human epithelial cells

Output Feedback Variable Structure Control Design for Uncertain Nonlinear Lipschitz Systems

This paper develops a full-order compensator-based output feedback variable structure control law for uncertain nonlinear Lipschitz systems having matched perturbations. Given that the sufficient condition is satisfied, the developed control scheme, with the observer-like technique incorporated into the design of the compensator, can achieve global exponential stabilization. An illustrative example is provided with simulation results to show the effectiveness of the proposed method