Activation peptide of the coagulation factor XIII (AP-F13A1) as a new biomarker for the screening of colorectal cancer

International audienceBackground: Colorectal cancer (CRC) remains a major cause of cancer fatalities in developed countries. The risk of death is correlated to the stage of CRC during the primary diagnosis. Early diagnosis is closely associated with enhanced survival rate. We therefore investigated the AP-F13A1 as a potential protein marker of CRC. Methods: The protein expression of FXIII in 40 serum samples was evaluated by enzyme-linked immunosorbent assays. Additionally, targeted proteomic assays (LC-PRM) were used to evaluate the expression of the activation peptide of F13A1 (AP-F13A1) in a further 113 serum samples. Results were analyzed by the Wilcoxon test and receiver operating characteristic curves generated to assess statistical differences and diagnostic factors between CRC patients and controls. Results: AP-F13A1 was quantified in human serum samples using calibration curves with excellent linearity. AP-F13A1 was reduced in CRC patients using PRM assays from two distinct biobanks. The AUC for AP-F13A1 were 0.95 and 0.93. Sensitivity/specificity values for the two sets of patients were 75%/95% and 71%/95% respectively. Conclusion: We have presented the proof of principle that in vivo release of AP-F13A1 can be measured by PRM-based strategies in CRC serum samples. AP-F13A1 may be an effective serological biomarker as part of a screening program of CRC detection

IDENTIFICATION PAR CDNA DIFFERENTIAL DISPLAY DE NOUVEAUX GENES IMPLIQUES DANS L'APOPTOSE INDUITE PAR LA PROTEINE P53 ETUDE FONCTIONNELLE DE LA PROTEINE HUMSIAH ET DE LA PRESENILINE-1 DANS LA SUPPRESSION TUMORALE

PARIS-BIUSJ-Physique recherche (751052113) / SudocCentre Technique Livre Ens. Sup. (774682301) / SudocSudocFranceF

Widespread glasses generated by cometary fireballs during the late Pleistocene in the Atacama Desert, Chile: COMMENT

International audienc

Surface vitrification caused by natural fires in Late Pleistocene wetlandsof the Atacama Desert

International audienceMelted rocks are a common feature in many of the 175 recognized terrestrial impact structures [1]. However, someglasses, like the Dakhleh Glass [2] or the Edeowie Glass [3] are also attributed to impacts despite the lack of otherdirect evidence. These cases have been attributed to low-altitude airbursts of cosmic bodies (asteroids, comets)during their entry in the Earth’s atmosphere but the identification and mechanism of formation of these glasses arehowever debated.Massive glass blocks were recently discovered [4] in the Tamarugal-Llamara basin of the Atacama desert in Chile.We show that these glasses, found near the town of Pica at four localities separated by up to 70 km, are neitherfulgurites, nor volcanic glasses, nor metallurgical slags related to anthropic activity, but show close similaritieswith other glasses, which have been attributed to large airbursts. However, most glasses contain numerous plantimprints and some glasses are mainly made of partially melted silicified plant twigs and field observations indicatethat the glasses are restricted to specific Late Pleistocene wetlands. Large oases did indeed form in the hyperaridAtacama desert due to elevated groundwater discharge and increased recharge during the Central Andean PluvialEvent (roughly coeval with the Mystery interval and Younger Dryas). 14C dating and paleomagnetic dataindicate that the glasses were formed during at least two distinct periods. The strong environmental control onthe distribution of the glasses and large differences in ages rule out the hypothesis of a single large airburst asthe cause of surface melting. The available data suggest that the Atacama desert surface glasses were formedin situ by natural fires in soils rich in dry organic matter and siliceous biological remains, at a time of strongclimate oscillations between wet (organic matter accumulation in soils) and dry periods (triggering fires) in desertwetlands.Our interpretation likely applies to other cases of silicate glasses attributed to airbursts, challenging the highairburst rate as well as the interpretation of fossils and organic matter in “impact glasses” [5]. Our study alsodemonstrates that exotic mineralogy with glass containing spherules of iron sulphides, metallic iron or ironphosphides may not necessarily imply an impact origin. This in turn cast doubts on some studies relating impactand climate change triggering the Younger Dryas cold event at the end of the Pleistocene [6].1. French, B. M. & Koeberl, C. Earth-Sci. Rev. 98, 123–170 (2010).2. Osinski, G. R. et al. Meteorit. Planet. Sci. 43, 2089–2107 (2008).3. Haines, P. W., Jenkins, R. J. F. & Kelley, S. P. Geology 29, 899 (2001).4. Blanco, N. & Tomlinsson, A. J. Carta Guatacondo, Región de Tarapacá. (2013).5. Schultz, P. H., Harris, R. S., Clemett, S. J., Thomas-Keprta, K. L. & Zarate, M. Geology 42, 515–518 (2014).6. Firestone, R. B. et al. Proc. Natl. Acad. Sci. 104, 16016–16021 (200

Surface vitrification caused by natural fires in Late Pleistocene wetlandsof the Atacama Desert

International audienceMelted rocks are a common feature in many of the 175 recognized terrestrial impact structures [1]. However, someglasses, like the Dakhleh Glass [2] or the Edeowie Glass [3] are also attributed to impacts despite the lack of otherdirect evidence. These cases have been attributed to low-altitude airbursts of cosmic bodies (asteroids, comets)during their entry in the Earth’s atmosphere but the identification and mechanism of formation of these glasses arehowever debated.Massive glass blocks were recently discovered [4] in the Tamarugal-Llamara basin of the Atacama desert in Chile.We show that these glasses, found near the town of Pica at four localities separated by up to 70 km, are neitherfulgurites, nor volcanic glasses, nor metallurgical slags related to anthropic activity, but show close similaritieswith other glasses, which have been attributed to large airbursts. However, most glasses contain numerous plantimprints and some glasses are mainly made of partially melted silicified plant twigs and field observations indicatethat the glasses are restricted to specific Late Pleistocene wetlands. Large oases did indeed form in the hyperaridAtacama desert due to elevated groundwater discharge and increased recharge during the Central Andean PluvialEvent (roughly coeval with the Mystery interval and Younger Dryas). 14C dating and paleomagnetic dataindicate that the glasses were formed during at least two distinct periods. The strong environmental control onthe distribution of the glasses and large differences in ages rule out the hypothesis of a single large airburst asthe cause of surface melting. The available data suggest that the Atacama desert surface glasses were formedin situ by natural fires in soils rich in dry organic matter and siliceous biological remains, at a time of strongclimate oscillations between wet (organic matter accumulation in soils) and dry periods (triggering fires) in desertwetlands.Our interpretation likely applies to other cases of silicate glasses attributed to airbursts, challenging the highairburst rate as well as the interpretation of fossils and organic matter in “impact glasses” [5]. Our study alsodemonstrates that exotic mineralogy with glass containing spherules of iron sulphides, metallic iron or ironphosphides may not necessarily imply an impact origin. This in turn cast doubts on some studies relating impactand climate change triggering the Younger Dryas cold event at the end of the Pleistocene [6].1. French, B. M. & Koeberl, C. Earth-Sci. Rev. 98, 123–170 (2010).2. Osinski, G. R. et al. Meteorit. Planet. Sci. 43, 2089–2107 (2008).3. Haines, P. W., Jenkins, R. J. F. & Kelley, S. P. Geology 29, 899 (2001).4. Blanco, N. & Tomlinsson, A. J. Carta Guatacondo, Región de Tarapacá. (2013).5. Schultz, P. H., Harris, R. S., Clemett, S. J., Thomas-Keprta, K. L. & Zarate, M. Geology 42, 515–518 (2014).6. Firestone, R. B. et al. Proc. Natl. Acad. Sci. 104, 16016–16021 (200

Surface vitrification caused by natural fires in Late Pleistocene wetlands of the Atacama Desert

International audience! Paleomagnetic laboratory-Geosciences Rennes-Measurements by P. Roperch ! Equipments 2G magnetometer-Molspin spinner-Agico JR6 ! 2G one measurement between two zeros : code C1 ! 2G four measurements between two zeros : code C4 ! Molspin spinner 6 positions : code Mo ! Jr6a spinner automated mode : code Ja ! Jr6/Jr5 spinner 2 positions : code J2 ! First measurement (or NRM) code N0, F0 or D0 in most cases ! MMTD Furnace for thermal demagnetization ! codes D+ and D-indicate that sample orientation changed in the furnace between ! two steps along +Z and-Z ! AF 2G Online three axis F+ = sequence coils X,Z,Y, F-= sequence coils Y,Z,X ! FX = after demagnetization along X; ! FY = along Y, FZ = along Z; combine FX,FY and FZ to remove GR

Architecture of low human and environmental impact.

Diante de um cenário de degradação ambiental global, escassez de matéria prima, água, energia, aumento da poluição, crise social e econômica, é feita a proposição de uma Arquitetura de Baixo Impacto Humano e Ambiental - ABIHA.Todas estas variáveis trazem novos elementos à Arquitetura aumentando a sua complexidade e fazendo com que haja necessidade de adaptação.Apesar de todas as incertezas e contradições envolvendo as questões de sustentabilidade, seus conceitos e sua aplicação na Arquitetura são abordados neste contexto.Além das questões conceituais, são apresentadas aplicações práticas da ABIHA, no Jardim Sustentável, na Reciclagem do Galpão da POLI e no MINI labiratório de Conforto e Eficiência Energética.Estas aplicações seriram de base para a proposta de Sistematização que é apresentada no final deste trabalho como parte das conclusões finais. Esta proposta é apenas início de muitas pesquisas que ainda devem ser feiras rumo a uma Arquitetura mais Sustentável.The actual global environmental context is one of fierce degradation : reflecting in the exhaustion of natural sources, increase of air, soil and water pollution, and social and economic crises.Facing such a scenario, it is proposed in this work, principles of architecture of low human environmental impact (arquitetura de baixo impacto humano e ambiental) - ABIHA.All these variables bring new parameters to the design of building, including their entire life cycles.Under these new ciecunstances it is observed an increae of the complexity in this design process, making necessary discussions for change.Despite all the uncertainties and contradictions about issues of sustainability, their concepts, as well as their applications are approached in the context of this work.Besides the conceptual matters, practions applications of ABIHA are carried out, in the example of the sustainable garden, warehouse recycling and the movable environmental laboratory (sensors and data lggers).Such experiences of practical application were fundamental to create the basis for the methodological assessment proposed as part of the final conclusions of this research.However, this procedure of evaluation is understood merely as the beginning of a big range of other researchs, which shlould be developed towards a more sustainable architecture