Dextran sulfate enhances the level of an oxidative DNA damage biomarker, 8-oxo-7,8-dihydro-2 0-deoxyguanosine, in rat colonic mucosa

Dextran sodium sulfate (DSS) given in drinking water can induce colonic Inflammation and produce colorectal tumors in rodents, although it is not directly genotoxic. The hypothesis that DSS can produce free radicals and induce oxidative DNA damage in colonic mucosa has been tested. In rats fed for 2 days with water containing 3% and 6% DSS, colonic Inflammation manifestations were recorded and 8-oxo-7,8-dihydro-2 0-deoxyguanosine (8-oxodGuo), a major biomarker of oxidative DNA damage, was assayed in colonic mucosa. As compared with control rats given pure water, inflammatory manifestations were seen in rats given DSS. At the same time, 8-oxodGuo levels in colonic mucosa were doubled (P , 0:001). These results suggest that formation of oxidative DNA damage in colonic mucosa depends on inflammation and maybe on the production of reactive oxygen species. This study shows that DSS can induce oxidative DNA damage within only 2 days, which could explain in part its carcinogenic properties

Tolerance analysis and synthesis by means of deviation domains, axi-symmetric cases

The small displacement torsors are generally used for the represeolation of the geometrical deviations. The standardised tolerances can then be translated by a set of inequalities between the components of a deviation torsor. hi me case of cylindrical possible to reduce the space to three dimensions at the maximum instead of six in the general case. Topological operations like the Minkowski sum to carry OUT the domains presented application relates to metro-logic inspection for a specification with maximum material condition on both the toleranced surface and the datum. The second example makes it possible to determine the deviation between two surfaces belonging to two different parts after mating them by two contact features

Electrical and optical measurements of the bandgap energy of a light-emitting diode

International audienceSemiconductors materials are at the basis of electronics. Most electronic devices are made of semiconductors. The operation of these components is well described by quantum physics which is a little mysterious to students. One of the intrinsic parameters of semiconductors is their bandgap energy Eg. In the case of light-emitting diodes (LEDs) Eg fixes the colour of the light emitted by the diodes. We propose in this article a lab work allowing a comparison of Eg of a green LED obtained by both electrical and optical measurements. The two slightly different results can be explained by the theoritical knowledges of the students on solid physics and electronic devices internal architecture

Introduction. L’agriculture en famille : travailler, réinventer, transmettre

En 2014, l’année internationale de l’agriculture familiale aura été l’occasion d’une abondante production scientifique sur les diverses problématiques que recouvre cette forme sociale d’agriculture très largement majoritaire sur la planète, en particulier sous forme d’ouvrages(Bélières et al., 2014 ; Bosc et al. (à paraître) ; Guétat-Bernard, 2014 ; Sourisseau, 2014). Les travaux ne manquent pas non plus par le passé, que ce soit sur l’agriculture familiale (Caron et Sabourin, 2001 ; Lamarche, 1992, 1994 ; Mazoyer et Roudard, 1997) ou plus spécifiquement sur l’exploitation agricole familiale (Barthez, 2003 ; Brossier et al., 2003 ; Chia et Dugué, 2006 ; Dedieu et al., 2008 ; Gafsi et al., 2007 ; Laurent et Rémy, 2000 ; Mayaud, 1999 ; Penot, 2012 ; Rémy, 2013). En France comme ailleurs, les recherches sur l’exploitation agricole interrogent régulièrement son caractère familial, sa permanence, ses recompositions ou son effacement (Hervieu et Purseigle, 2013 ; Mundler et Rémy, 2012 ; Rémy, 2011). Unité de base de recensement et de création de données statistiques (Barthez, 1986 ; Laurent et al., 1994), l’exploitation est aussi une cible privilégiée des politiques publiques agricoles, rurales, environnementales, et désormais alimentaires, ce qui en fait un objet de connaissance de premier ordre. Dans ce contexte, quelles sont les ambitions de ce nouvel ouvrage sur l’exploitation, l’agriculteur et l’activité agricole en famille

Two-dimensional ultrasonic computed tomography of growth bone

International audienceUltrasonography is the main first-line imaging technique used to diagnose various pediatric pathologies. Pediatric radiologists are very keen on ultrasonography since it is a non-invasive, non-irradiant, painless, inexpensive imaging modality, which is also practicable on bedside. In bone diseases, this technique has also proved to be a very effective tool to assess congenital (i.e., hip dysphasia), infectious (i.e., sub-periostal abscess), inflammatory (i.e., chronic arthritis), and even traumatic (i.e., ankle sprain) processes. However, with standard devices, this exam is not suitable for diagnosis or monitoring bone tumors, classically explored by other more effective techniques, which presented various disadvantages (cost, irradiation, sedation, accessibility). Many authors have dealt with the ultrasonic imaging of bones. Their main aim has usually been to assess the thickness of the diaphysis and to calculate the speed of sound of a wave crossing the structure. Our group has been focusing on the cross-sectional radial imaging process, using ultrasonic computed tomography. Known to be a potentially valuable method of imaging objects with a similar acoustical impedance, difficulties arise when it is proposed to obtain quantitative tomograms of more highly contrasted media (hard bone tissues). Finding solutions here involves either using non-linear schemes and/or performing extensive studies on the limitation of the initial approximation. In this paper, we recall the limits of the ultrasonic computed tomography when dealing with highly contrasted scatterers, using a high-order tomographic method. The performances and limitations are evaluated in the basis of simulated and experimental data, obtained using an ultrasonic scanner, to one geometrical-mimicking phantom and to a real children fibula. Sample thicknesses and speed-of-sound varied as a function of radial position and the experimental procedure fulfilled the criteria of the diffraction tomography. Results are promising and suggest that the geometrical and acoustical characteristics of the bones, which are known to be one of the main factors contributing to the growth bone disease, can be determined using this ultrasonic computed tomography

Evolutionary history of the alpha2,8-sialyltransferase (ST8Sia) gene family: Tandem duplications in early deuterostomes explain most of the diversity found in the vertebrate ST8Sia genes

<p>Abstract</p> <p>Background</p> <p>The animal sialyltransferases, which catalyze the transfer of sialic acid to the glycan moiety of glycoconjugates, are subdivided into four families: ST3Gal, ST6Gal, ST6GalNAc and ST8Sia, based on acceptor sugar specificity and glycosidic linkage formed. Despite low overall sequence identity between each sialyltransferase family, all sialyltransferases share four conserved peptide motifs (L, S, III and VS) that serve as hallmarks for the identification of the sialyltransferases. Currently, twenty subfamilies have been described in mammals and birds. Examples of the four sialyltransferase families have also been found in invertebrates. Focusing on the ST8Sia family, we investigated the origin of the three groups of α2,8-sialyltransferases demonstrated in vertebrates to carry out poly-, oligo- and mono-α2,8-sialylation.</p> <p>Results</p> <p>We identified in the genome of invertebrate deuterostomes, orthologs to the common ancestor for each of the three vertebrate ST8Sia groups and a set of novel genes named ST8Sia EX, not found in vertebrates. All these ST8Sia sequences share a new conserved family-motif, named "C-term" that is involved in protein folding, via an intramolecular disulfide bridge. Interestingly, sequences from <it>Branchiostoma floridae </it>orthologous to the common ancestor of polysialyltransferases possess a polysialyltransferase domain (PSTD) and those orthologous to the common ancestor of oligosialyltransferases possess a new ST8Sia III-specific motif similar to the PSTD. In osteichthyans, we have identified two new subfamilies. In addition, we describe the expression profile of ST8Sia genes in <it>Danio rerio</it>.</p> <p>Conclusion</p> <p>Polysialylation appeared early in the deuterostome lineage. The recent release of several deuterostome genome databases and paralogons combined with synteny analysis allowed us to obtain insight into events at the gene level that led to the diversification of the ST8Sia genes, with their corresponding enzymatic activities, in both invertebrates and vertebrates. The initial expansion and subsequent divergence of the ST8Sia genes resulted as a consequence of a series of ancient duplications and translocations in the invertebrate genome long before the emergence of vertebrates. A second subset of ST8sia genes in the vertebrate genome arose from whole genome duplication (WGD) R1 and R2. Subsequent selective ST8Sia gene loss is responsible for the characteristic ST8Sia gene expression pattern observed today in individual species.</p

Estimated stress and friction distributions on tool rake face in the medium density fiberboard cutting process

This paper presents a model of load distribution on the cutting edge of a tool during machining Medium Density Fibreboard (MDF). A series of cutting tests has been carried out with tools having designated rake face contact lengths. Utilising the experimental data and a mechanics approach developed earlier by the third author, a model to estimate the distribution of stresses and friction on the rake face is developed. The model provides an essential step in the design and development of cutting edge geometry to prevent early edge failure and to control and reduce thermal/mechanical loading of the tool wedge

The Dual Origin Of The Nitrogen Deficiency In Comets: Selective Volatile Trapping In The Nebula And Postaccretion Radiogenic Heating

We propose a scenario that explains the apparent nitrogen deficiency in comets in away that is consistent with the fact that the surfaces of Pluto and Triton are dominated by nitrogen-rich ice. We use a statistical thermodynamic model to investigate the composition of the successive multiple guest clathrates that may have formed during the cooling of the primordial nebula from the most abundant volatiles present in the gas phase. These clathrates agglomerated with the other ices (pure condensates or stoichiometric hydrates) and formed the building blocks of comets. We report that molecular nitrogen is a poor clathrate former, when we consider a plausible gas-phase composition of the primordial nebula. This implies that its trapping into cometesimals requires a low disk temperature (similar to 20 K) in order to allow the formation of its pure condensate. We find that it is possible to explain the lack of molecular nitrogen in comets as a consequence of their postformation internal heating engendered by the decay of short-lived radiogenic nuclides. This scenario is found to be consistent with the presence of nitrogen-rich ice covers on Pluto and Triton. Our model predicts that comets should present xenon-to-water and krypton-to-water ratios close to solar xenon-to-oxygen and krypton-to-oxygen ratios, respectively. In contrast, the argon-to-water ratio is predicted to be depleted by a factor of similar to 300 in comets compared to solar argon-to-oxygen, as a consequence of poor trapping efficiency and radiogenic heating.CNESJPLAstronom

An introduction to photocatalysis through methylene blue photodegradation

International audienceWe described a simple experimental setup for a lab work on the photocatalytic degradation of methylene blue by T iO 2 nanoparticles. The photocatalysis process can be used for many applications. Treatments for diluted wastewaters industries, air purifying in underground car parks, preventing fouling on glass surfaces, these are some of the things this phenomenon has the ability to do. The described experiment is easy to perform and the interpretation can be easily adapted to different level of students: from high school students demonstrating their interests on a sustainable development, to students who are getting Masters in the science department that want to propose a full explanation of all the phenomenon of the photocatalytic process. Starting by a description of the experimental setup , we analysed the photocatalyst nanoparticles and applied the Langmuir-Hinshelwood model to our experimental data. Finally we discussed shortly on the respective energetic levels of the photocatalyst semiconductor and the methylene blue