The Effect of Fertilizer Treatments at Three Compactness Levels on Qualitative Traits of Sport Lawn in Winter Season

Lawn quality, which introduces by good color, density, uniformity and texture varies depending on the species, maintenance operation, and time of the year. In the current study, the effect of organic fertilizers containing Leaf Mold (LM), Rice Husk (RH), manure, Spent Mushroom Compost (SMC), a mixture of LM, RH and SMC (mixture 1), a mixture of LM, RH and manure (mixture 2), with the ratio of 1:1:1 and control (no fertilizer) at three levels of soil compactness including roller weights of 36, 56, 76 kilograms on qualitative traits of sport lawn were investigated in winter season. Treatments were applied in a strip plot design with three replications, in research farm of Gorgan University of Agricultural Sciences and Natural Resources during fall 2008-2009. According to achieved results, manure and RH treatments in first and second compaction and manure treatment in third compaction level showed the highest amount of chlorophyll content. In visual assessment, the highest and lowest of lawn quality was observed in the manure and the control treatment respectively. In first and second compaction, the maximum height was observed in manure treatment. Control and LM treatments showed the minimum height. In third compaction, maximum and minimum heights were associated with manure and control treatment respectively

Effect of Several Organic Fertilizers Along with Some Soil Compactness Levels on Nutrients Content of Sport Lawn in Fall Season

The lawn has an incredible role in designing and construction of landscape areas and lawn bed provides aeration, moisture and nutrients, which is essential for having an acceptance lawn, so the role of organic matter and degree of soil compactness need to be studied. In the current study, the effect of some bed mixtures including Leaf Mold (LM), Rice Husk (RH), manure, Spent Mushroom Compost (SMC), a mixture of LM, RH and SMC (mixture 1), a mixture of LM, RH and manure (mixture 2) with the ratio of 1:1:1 and control (no fertilizer), also the effect of some different soil compactness (roller weights of 36, 56 and 76 kilograms) on the content of some nutrients of sport lawn in fall season were investigated. Hence, an experiment was conducted at the strip plot design with three replications, in research farm of Gorgan University of Agricultural Sciences and Natural Resources during 2008-09. According to the results of this study, interaction of fertilizer and soil compactness was significant for most measured factors. The manure and control treatments showed the most and the least amount of nitrogen in all compactness treatments respectively. The manure and SMC treatments and also treatments containing these organic matters increased plant phosphorus content in comparing with RH, LM and control treatments. Also in three compactness, manure and SMC treatments showed more plant potassium in contrast to control

Applying a User-centred Approach to Interactive Visualization Design

Analysing users in their context of work and finding out how and why they use different information resources is essential to provide interactive visualisation systems that match their goals and needs. Designers should actively involve the intended users throughout the whole process. This chapter presents a user-centered approach for the design of interactive visualisation systems. We describe three phases of the iterative visualisation design process: the early envisioning phase, the global specification hase, and the detailed specification phase. The whole design cycle is repeated until some criterion of success is reached. We discuss different techniques for the analysis of users, their tasks and domain. Subsequently, the design of prototypes and evaluation methods in visualisation practice are presented. Finally, we discuss the practical challenges in design and evaluation of collaborative visualisation environments. Our own case studies and those of others are used throughout the whole chapter to illustrate various approaches

Differential usage of transcriptional start sites and polyadenylation sites in FMR1 premutation alleles†

5′- and 3′-untranslated regions (UTRs) are important regulators of gene expression and play key roles in disease progression and susceptibility. The 5′-UTR of the fragile X mental retardation 1 (FMR1) gene contains a CGG repeat element that is expanded (>200 CGG repeats; full mutation) and methylated in fragile X syndrome (FXS), the most common form of inherited intellectual disability (ID) and known cause of autism. Significant phenotypic involvement has also emerged in some individuals with the premutation (55–200 CGG repeats), including fragile X-associated premature ovarian insufficiency (FXPOI) in females, and the neurodegenerative disorder, fragile X-associated tremor/ataxia syndrome (FXTAS), in older adult carriers. Here, we show that FMR1 mRNA in human and mouse brain is expressed as a combination of multiple isoforms that use alternative transcriptional start sites and different polyadenylation sites. Furthermore, we have identified a novel human transcription start site used in brain but not in lymphoblastoid cells, and have detected FMR1 isoforms generated through the use of both canonical and non-canonical polyadenylation signals. Importantly, in both human and mouse, a specific regulation of the UTRs is observed in brain of FMR1 premutation alleles, suggesting that the transcript variants may play a role in premutation-related pathologies

Interaction of the Transcription Start Site Core Region and Transcription Factor YY1 Determine Ascorbate Transporter SVCT2 Exon 1a Promoter Activity

Transcription of the ascorbate transporter, SVCT2, is driven by two distinct promoters in exon 1 of the transporter sequence. The exon 1a promoter lacks a classical transcription start site and little is known about regulation of promoter activity in the transcription start site core (TSSC) region. Here we present evidence that the TSSC binds the multifunctional initiator-binding protein YY1. Electrophoresis shift assays using YY1 antibody showed that YY1 is present as one of two major complexes that specifically bind to the TSSC. The other complex contains the transcription factor NF-Y. Mutations in the TSSC that decreased YY1 binding also impaired the exon 1a promoter activity despite the presence of an upstream activating NF-Y/USF complex, suggesting that YY1 is involved in the regulation of the exon 1a transcription. Furthermore, YY1 interaction with NF-Y and/or USF synergistically enhanced the exon 1a promoter activity in transient transfections and co-activator p300 enhanced their synergistic activation. We propose that the TSSC plays a vital role in the exon 1a transcription and that this function is partially carried out by the transcription factor YY1. Moreover, co-activator p300 might be able to synergistically enhance the TSSC function via a “bridge” mechanism with upstream sequences

Comparative analysis of the ATRX promoter and 5' regulatory region reveals conserved regulatory elements which are linked to roles in neurodevelopment, alpha-globin regulation and testicular function

BACKGROUND ATRX is a tightly-regulated multifunctional protein with crucial roles in mammalian development. Mutations in the ATRX gene cause ATR-X syndrome, an X-linked recessive developmental disorder resulting in severe mental retardation and mild alpha-thalassemia with facial, skeletal and genital abnormalities. Although ubiquitously expressed the clinical features of the syndrome indicate that ATRX is not likely to be a global regulator of gene expression but involved in regulating specific target genes. The regulation of ATRX expression is not well understood and this is reflected by the current lack of identified upstream regulators. The availability of genomic data from a range of species and the very highly conserved 5' regulatory regions of the ATRX gene has allowed us to investigate putative transcription factor binding sites (TFBSs) in evolutionarily conserved regions of the mammalian ATRX promoter. RESULTS We identified 12 highly conserved TFBSs of key gene regulators involved in biologically relevant processes such as neural and testis development and alpha-globin regulation. CONCLUSIONS Our results reveal potentially important regulatory elements in the ATRX gene which may lead to the identification of upstream regulators of ATRX and aid in the understanding of the molecular mechanisms that underlie ATR-X syndrome.This work was supported by Department of Zoology research grants