Response of rat hindlimb muscles to 12 hours recovery from tail-cast suspension

Previous work has shown a number of biochemical changes which accompany atrophy or reduced muscle growth in hindlimb of tail-casted, suspended rats. These results clearly show that altered muscle growth was due to changes in protein turnover. Accordingly, the rise in soleus tyrosine following unloading reflects the more negative protein balance. Other major changes we found included slower synthesis of glutamine as indicated by lower ratios of glutamine/glutamate and reduced levels of aspartate which coincide with slower aspartate and ammonia metabolism in vitro. In conjunction with the study of SL-3 rats, which were subjected to 12 h of post-flight gravity, a study of the effects of 12 h eight bearing on metabolism of 6-day unloaded hindlimb muscles was carried out

Responses of skeletal muscle to unloading, a review

Suspension models were used to study muscle response to reduced activity. During 6 days of tail casting, the soleus (SOL) atrophies while the extensor digitorum longus grows relatively normally. After discounting those changes in both muscles due primarily to increased secretion of adrenal hormones, the following conclusions regarding the specific responses of the SOL could be drawn: (1) Atrophy is probably due primarily to increased protein degradation; (2) Decreased synthesis of glutamine may result from reduced availability of ammonia due to diminished use of ATP; (3) Greater muscle glycogen seems to reflect an increased response to insulin of glucose uptake which leads to greater glucose metabolism; and (4) Faster catabolism of branched-chain amino acids can be attributed to enhanced flux through ketoacid dehydrogenase. Studies by others using tail casted suspended rats showed in the SOL: (1) a gradual switch from type 1 to type 2 fibers; (2) increased acid protease activity; and (3) altered muscle function and contractile duration. Using harness suspended rats, others showed in the SOL: (1) significant atrophy; (2) increased numbers of glucocorticoid receptors; and (3) no change in muscle fatigability

Why the idea of framework propositions cannot contribute to an understanding of delusions

One of the tasks that recent philosophy of psychiatry has taken upon itself is to extend the range of understanding to some of those aspects of psychopathology that Jaspers deemed beyond its limits. Given the fundamental difficulties of offering a literal interpretation of the contents of primary delusions, a number of alternative strategies have been put forward including regarding them as abnormal versions of framework propositions described by Wittgenstein in On Certainty. But although framework propositions share some of the apparent epistemic features of primary delusions, their role in partially constituting the sense of inquiry rules out their role in helping to understand delusions

Critical behaviour in the nonlinear elastic response of hydrogels

In this paper we study the elastic response of synthetic hydrogels to an applied shear stress. The hydrogels studied here have previously been shown to mimic the behaviour of biopolymer networks when they are sufficiently far above the gel point. We show that near the gel point they exhibit an elastic response that is consistent with the predicted critical behaviour of networks near or below the isostatic point of marginal stability. This point separates rigid and floppy states, distinguished by the presence or absence of finite linear elastic moduli. Recent theoretical work has also focused on the response of such networks to finite or large deformations, both near and below the isostatic point. Despite this interest, experimental evidence for the existence of criticality in such networks has been lacking. Using computer simulations, we identify critical signatures in the mechanical response of sub-isostatic networks as a function of applied shear stress. We also present experimental evidence consistent with these predictions. Furthermore, our results show the existence of two distinct critical regimes, one of which arises from the nonlinear stretch response of semi-flexible polymers.

Can kinesiophobia predict the duration of neck symptoms in acute whiplash?

Objectives: In low back pain, clinical studies suggest that kinesiophobia (fear of movement/(re)injury) is important in the etiology of chronic symptoms. In this prospective cohort study, the predictive role of kinesiophobia in the development of late whiplash syndrome was examined. Methods: Victims of car collisions with neck symptoms who initiated compensation claim procedures with a Dutch insurance company were sent a questionnaire containing symptom-related questions and the Tampa Scale of Kinesiophobia (TSK-DV). Follow-up questionnaires were administered 6 and 12 months after the collision. Survival analysis was used to study the relationship between the duration of neck symptoms and explanatory variables. Results: Of the 889 questionnaires sent, 590 (66%) were returned and 367 used for analysis. The estimated percentage Of Subjects with neck symptoms persisting 1 year after the collision was 47% (SE 2.7%). In a regression model without symptom-related variables, kinesiophobia was found to be related to a longer duration of neck symptoms (P = 0.001). However, when symptom-related information was entered into the model, the effect of kinesiophobia did not reach statistical significance (P = 0.089). Conclusions: Although a higher score on the TSK-DV was found to be associated with a longer duration of neck symptoms, information on early kinesiophobia was not found to improve the ability to predict the duration of neck symptoms after motor vehicle collisions

Interactions of gelatinous zooplankton within marine food webs

Gelatinous zooplankton (GZ) comprise a taxonomically and functionally diverse group of marine organisms which includes ctenophores, cnidarians and pelagic tunicates, sharing a soft, mostly transparent body texture, a high body water content and a lack of exoskeleton. They range in size from less than a millimetre to nearly 2 m for the cnidarian jellyfish Nemopilema nomurai, and comprise some of the fastest growing metazoans on Earth (Hopcroft et al., 1998), sometimes surpassing crustacean zooplankton in their contribution to secondary production (i.e. in subtropical waters; Jaspers et al., 2009). They feed on a wide range of prey sizes, with predator–prey ratios comparable in some cases to those of baleen whales and krill (Deibel and Lee, 1992), and with prey removal rates which are similar to those of their non-gelatinous competitors (Acuña et al., 2011). In spite of early work pointing to gelatinous zooplankton as a trophic dead end (Verity and Smetacek, 1996), evidence is rapidly accumulating which shows that they may potentially channel energy from the picoplankton-sized, microbial loop organisms up to the higher trophic levels, including fish (Llopiz et al., 2010). However, this pathway is still largely neglected in most food web investigations even though it is now becoming clear that GZ represent a major fraction of the diet of several commercially important fish species such as bluefin tuna (Thunnus thynnus) (Cardona et al., 2012)

Carbon content of Mnemiopsis leidyi eggs and specific egg production rates in northern Europe

The comb jelly Mnemiopsis leidyi is considered to be a successful invasive species, partly due to its high reproduction potential. However, due to the absence of direct carbon measurements of eggs, specific reproduction rates remain uncertain. We show that egg carbon is 0.22 ± 0.02 µg C and up to 21 times higher than previously extrapolated. With maximum rates of 11 232 eggs ind−1 day−1, largest animals in northern Europe invest ∼10% day−1 of their body carbon into reproduction. The comb jelly M. leidyi has received wide public and scientific attention during the last decades due to its commonly observed formation of bloom abundances in native and invaded areas (e.g. Costello et al., 2012; Riisgård et al., 2012). One of the traits suggested to be responsible for M. leidyi's invasion success is its high fecundity. At the northern end of its distribution range in native areas, M. leidyi has been shown to produce up to 9380 and 14 233 eggs ind−1 day−1 (Kremer, 1976a; Graham et al., 2009), with similar rates of 9910 eggs ind−1 day−1 for the native southern population in Biscayne Bay, FL, USA (Baker and Reeve, 1974). Within invaded European waters, rates of up to 3000 and 12 000 eggs ind−1 day−1 have been recorded for northern and southern populations, respectively (Zaika and Revkov, 1994; Javidpour et al., 2009). Since M. leidyi is a simultaneous hermaphrodite and fertilized eggs are produced on a daily basis during favorable conditions (Jaspers, 2012), M. leidyi can circumvent the Allee effect and efficiently seed new populations even from few founding individuals. Although