Selectivity of DNA polymerases toward α and β nucleotide substrates of d and l series

AbstractThe substrate properties of four carbocyclic d and l nucleoside 5′-triphosphate analogs toward HIV and AMV reverse transcriptases and terminal deoxynucleotidyl transferase were evaluated. The compounds of the d-β and l-β series were found to be terminating substrates for these enzymes, while the derivatives of the d-α and l-α series were recognized only by terminal deoxynucleotidyl transferase, suggesting that for the template-independent enzyme the mutual orientation of the two fragments is of no significance. A hypothesis for binding of nucleotides to the DNA polymerase active center was proposed

OMERACT Definitions for Ultrasonographic Pathology and Elementary Lesions Of Rheumatic Disorders Fifteen Years On

Objective. The Outcome Measures in Rheumatology (OMERACT) ultrasound (US) working group (WG) operates research activities for the validation of US as an outcome measurement instrument according to the Filter 2.0 framework Methods. From the onset of the WG research in 2005 through now, original publications on definitions and scoring systems for pathophysiological manifestations and elementary lesions of various rheumatic disorders were reviewed Results. Definitions and scoring systems according to new terminology are provided Conclusions. We have redefined OMERACT definitions of US pathology and elementary lesions as well as scoring systems which are now proposed for OMERACT approval for application in clinical trial

The 2017 EULAR standardised procedures for ultrasound imaging in rheumatology

BACKGROUND: In 2001, the European League Against Rheumatism developed and disseminated the first guidelines for musculoskeletal (MS) ultrasound (US) in rheumatology. Fifteen years later, the dramatic expansion of new data on MSUS in the literature coupled with technological developments in US imaging has necessitated an update of these guidelines.OBJECTIVES: To update the existing MSUS guidelines in rheumatology as well as to extend their scope to other anatomic structures relevant for rheumatology.METHODS: The project consisted of the following steps: (1) a systematic literature review of MSUS evaluable structures; (2) a Delphi survey among rheumatologist and radiologist experts in MSUS to select MS and non-MS anatomic structures evaluable by US that are relevant to rheumatology, to select abnormalities evaluable by US and to prioritise these pathologies for rheumatology and (3) a nominal group technique to achieve consensus on the US scanning procedures and to produce an electronic illustrated manual (ie, App of these procedures).RESULTS: Structures from nine MS and non-MS areas (ie, shoulder, elbow, wrist and hand, hip, knee, ankle and foot, peripheral nerves, salivary glands and vessels) were selected for MSUS in rheumatic and musculoskeletal diseases (RMD) and their detailed scanning procedures (ie, patient position, probe placement, scanning method and bony/other landmarks) were used to produce the App. In addition, US evaluable abnormalities present in RMD for each anatomic structure and their relevance for rheumatology were agreed on by the MSUS experts.CONCLUSIONS: This task force has produced a consensus-based comprehensive and practical framework on standardised procedures for MSUS imaging in rheumatology

Global to local perspectives of early childhood education and care

peerreview_statement: The publishing and review policy for this title is described in its Aims & Scope. aims_and_scope_url: http://www.tandfonline.com/action/journalInformation?show=aimsScope&journalCode=gecd2

Specjacyjne formy fosforu w osadach dennych trzech wybranych zbiorników antropogenicznych o różnym stopniu trofii

The study presents the results of the research into different phosphorus forms in the bottom sediments of anthropogenic limnic ecosystems i.e. the reservoirs of Pławniowice, Rybnik and Goczałkowice (SP). The bottom sediments of dam reservoirs were investigated by chemical extraction procedure for phosphorus forms. The lowest value of the mean AAP form percentage in the Pławniowice bottom sediments reflected the effect of reclamation with the hypolimnetic removal that had been conducted in the reservoir since 2003. The highest percentage of the RDP form (2%) was found in the Goczałkowice bottom sediments. The order of the specific speciation forms in the bottom sediments of the examined reservoirs was: Rybnik: AAP > EP > WDP > RDP; 4,630> 3,740 > 117 > 65 > 3.5 mgP/kg Pławniowice: AAP > EP > WDP > RDP; 916 > 783 > 107 > 15 > 1.4 mgP/kg Goczałkowice: AAP > WDP > EP > RDP; 686 > 628 > 51 > 7 > 0.14 mgP/kg The mutual correlations between the phosphorus speciation forms (AAP : EP : WDP : RDP) were as follows: Rybnik: 1,323 : 1,068 : 33 : 18 : 1; Pławniowice: 654 : 559 : 76 : 11 : 1; Goczałkowice: 4,900 : 4,485 : 364 : 50 : 1. The comparison of the mean concentration values for specific phosphorus forms in the bottom sediments of the three investigated reservoirs demonstrated that the Rybnik sediments had the highest contents of phosphorus. The contents in Pławniowice and Goczałkowice were 5–7 times lower.Przedstawiono wyniki badań specjacyjnych form fosforu w osadach dennych antropogenicznych ekosystemów limnicznych. W badaniach wykorzystano chemiczną ekstraktację form fosforu oraz zasady analizy biodostępności. Osady zbiorników Pławniowice i Goczałkowice zawierają około 5–7 razy mniej fosforu niż zbiornik Rybnicki. Najniższa wartość średniego udziału formy AAP w osadach dennych zbiornika Pławniowice odzwierciedla skutek prowadzonej od 2003 roku rekultywacji tego zbiornika metoda usuwania hypolimnionu. Najwyższa ze wszystkich trzech zbiorników wartość udziału (2%) formy RDP, w osadach dennych zbiornika Goczałkowice może wskazywać na silniejsze właściwości sorpcyjne materii tworzącej osady tego zbiornika. Pod względem stężenia, kolejność form specjacyjnych w osadach dennych badanych zbiorników układała się: Zbiornik Rybnicki: AAP > EP > WDP > RDP; 4630.> 3740 > 117 > 65 > 3,5 mgP/kg Zbiornik Pławniowice: AAP > EP > WDP > RDP; 916 > 783 > 107 > 15 > 1,4 mgP/kd Zbiornik Goczałkowice: AAP > WDP > EP > RDP; 686 > 628 > 51 > 7 > 0,14 mgP/kg W zbiorniku Goczałkowice wyższe stężenie WDP od EP wskazuje niski potencjał kumulacyjny. Wzajemne relacje pomiędzy formami fosforu – AAP : EP : WDP : RDP – były następujące: Zbiornik Rybnicki: 1323 : 1068 : 33 : 18 : 1, Zbiornik Pławniowice: 654 : 559 : 76 : 11 : 1, Zbiornik Goczałkowice: 4900 : 4485 : 364 : 50 : 1. Wartości potencjału redox wskazują na środowisko redukcyjne w osadach zbiorników Pławniowice i Rybnik. Średnie stężenia specjacyjnych form fosforu w osadach dennych wykazało, że najbardziej zasobne w fosfor są osady zbiornika Rybnickiego