Native and Non-Native Speaker Judgements on the Quality of Synthesized Speech

The difference between native speakers' and non-native speak- ers' naturalness judgements of synthetic speech is investigated. Similar/difference judgements are analysed via a multidimen- sional scaling analysis and compared to Mean opinion scores. It is shown that although the two groups generally behave in a similar manner the variance of non-native speaker judgements is generally higher. While both groups of subject can clearly distinguish natural speech from the best synthetic examples, the groups' responses to different artefacts present in the synthetic speech can vary

Further exploration of the possibilities and pitfalls of multidimensional scaling as a tool for the evaluation of the quality of synthesized speech

Multidimensional scaling (MDS) has been suggested as a useful tool for the evaluation of the quality of synthesized speech. However, it has not yet been extensively tested for its applica- tion in this specific area of evaluation. In a series of experiments based on data from the Blizzard Challenge 2008 the relations between Weighted Euclidean Distance Scaling and Simple Euclidean Distance Scaling is investigated to understand how aggregating data affects the MDS configuration. These results are compared to those collected as mean opinion scores (MOS). The ranks correspond, and MOS can be predicted from an object's space in the MDS generated stimulus space. The big advantage of MDS over MOS is its diagnostic value; dimensions along which stimuli vary are not correlated, as is the case in modular evaluation using MOS. Finally, it will be attempted to generalize from the MDS representations of the thoroughly tested subset to the aggregated data of the larger-scale Blizzard Challenge

Video Montage on Android

Cílem této práce je implementovat aplikaci umožňující editování videa na platformě Android. Při implementaci byl použit framework FFmpeg umožňující manipulaci s multimediálním obsahem. Během implementace byla aplikace iterativně vylepšována a přizpůsobována požadavkům uživatelů. Výsledná aplikace umožňuje střih, spojování několika videí a~aplikování efektů. Díky aplikaci může potencionální uživatel sestříhat natočená videa během pár minut.The main goal of this thesis is to implement an application enabling video editing on the Android platform. The FFmpeg framework was used in the implementation. It enables manipulation with multimedia content. The application was iteratively improved and adapted to user requirements during implementation. The resulting application allows video cutting, merging several videos, and applying effects. Thanks to the application a potential user can edit recorded videos in a few minutes.

Further Investigation of MDS as a Tool for Evaluation of Speech Quality of Synthesized Speech

The dissertation investigates MDS as a tool for the evaluation of the quality of synthesized speech. More specifically, it investigates the relations between Weighted Euclidean Distance Scaling and Simple Euclidean Distance Scaling, and how aggregating data affects the MDS configuration. It is investigated to what extent a subset of experimental participants and/or experimental stimuli are representative of a larger test set. For that purpose an experiment was conducted on the basis of a subset of stimuli used in the Blizzard Challenge 2008. Issues in the evaluation of Speech Synthesis are discussed and an overview of the basics of multi-dimensional scaling is given to an extent that allows comprehension of methods used in the application of Multi-dimensional scaling to speech synthesis evaluation. Based on the experimental findings, further experiments are suggested with the goal in mind that testing procedures can be optimized to such an extent that the number of experimental participants can be drastically reduced

Asymmetries in the perception of synthesized speech

It was previously observed [1] that the order of presentation of paired stimuli influenced the number of different responses in same-different tasks in speech synthesis evaluation. This paper investigates this phenomenon within the context of cognitive psychology and demonstrates that, as the cognitive psychology literature suggests, there is an effect relating to the prototypicality of the stimulus. Index Terms: speech synthesis, evaluation, perception, Blizzard Challeng

Dopamine modulates dynamic decision-making during foraging

The mesolimbic dopaminergic system exerts a crucial influence on incentive processing. However, the contribution of dopamine in dynamic, ecological situations where reward rates vary, and decisions evolve over time, remains unclear. In such circumstances, current (foreground) reward accrual needs to be compared continuously with potential rewards that could be obtained by travelling elsewhere (background reward rate), in order to determine the opportunity cost of staying versus leaving. We hypothesised that dopamine specifically modulates the influence of background – but not foreground – reward information when making a dynamic comparison of these variables for optimal behaviour. On a novel foraging task based on an ecological account of animal behaviour (marginal value theorem), human participants of either sex decided when to leave locations in situations where foreground rewards depleted at different rates, either in rich or poor environments with high or low background rates. In line with theoretical accounts, people’s decisions to move from current locations were independently modulated by changes in both foreground and background reward rates. Pharmacological manipulation of dopamine D2 receptor activity using the agonist cabergoline significantly affected decisions to move on, specifically modulating the effect of background reward rates. In particular, when on cabergoline, people left patches in poor environments much earlier. These results demonstrate a role of dopamine in signalling the opportunity cost of rewards, not value per se. Using this ecologically derived framework we uncover a specific mechanism by which D2 dopamine receptor activity modulates decision-making when foreground and background reward rates are dynamically compared

Temperature and Photoperiod Effects on Sterility in a Cytoplasmic Male-Sterile Soybean

Manual cross-pollination to produce large quantities of hybrid soybean seed is difficult and time consuming. An environmentally stable sterility system is one of the necessary components to produce large quantities of hybrid seed. The objective of this study was to subject cytoplasmic male-sterile (CMS) BC5F1 plants, from a cross of a Chinese Glycine max wild-type soybean with a Chinese wild annual soybean G. soja (male parent) and controls, to a variety of different temperature and photoperiod treatments to test whether CMS is stable under various environmental conditions. Plants were grown in growth chambers under controlled temperature, photoperiod, and irradiance regimes until pod set, and then they were transferred to a glasshouse until they matured. Plants were evaluated for time of anthesis after photoperiod induction (13 h light/11 h dark) and fertility or sterility. Anther squash and pod set data showed that sterility of the CMS line was stable under all environmental conditions tested, whereas fertility-restored control plants remained fertile. Extreme environmental conditions led to delayed floral induction and/or stunted growth

CMG-Pol epsilon dynamics suggests a mechanism for the establishment of leading-strand synthesis in the eukaryotic replisome

The replisome unwinds and synthesizes DNA for genome duplication. In eukaryotes, the Cdc45–MCM–GINS (CMG) helicase and the leading-strand polymerase, Pol epsilon, form a stable assembly. The mechanism for coupling DNA unwinding with synthesis is starting to be elucidated, however the architecture and dynamics of the replication fork remain only partially understood, preventing a molecular understanding of chromosome replication. To address this issue, we conducted a systematic single-particle EM study on multiple permutations of the reconstituted CMG–Pol epsilon assembly. Pol epsilon contains two flexibly tethered lobes. The noncatalytic lobe is anchored to the motor of the helicase, whereas the polymerization domain extends toward the side of the helicase. We observe two alternate configurations of the DNA synthesis domain in the CMG-bound Pol epsilon. We propose that this conformational switch might control DNA template engagement and release, modulating replisome progression.FWN – Publicaties zonder aanstelling Universiteit Leide

Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome

Eukaryotic origin firing depends on assembly of the Cdc45-MCM-GINS (CMG) helicase. A key step is the recruitment of GINS that requires the leading-strand polymerase Pol epsilon, composed of Pol2, Dpb2, Dpb3, Dpb4. While a truncation of the catalytic N-terminal Pol2 supports cell division, Dpb2 and C-terminal Pol2 (C-Pol2) are essential for viability. Dpb2 and C-Pol2 are non-catalytic modules, shown or predicted to be related to an exonuclease and DNA polymerase, respectively. Here, we present the cryo-EM structure of the isolated C-Pol2/Dpb2 heterodimer, revealing that C-Pol2 contains a DNA polymerase fold. We also present the structure of CMG/C-Pol2/Dpb2 on a DNA fork, and find that polymerase binding changes both the helicase structure and fork-junction engagement. Intersubunit contacts that keep the helicase-polymerase complex together explain several cellular phenotypes. At least some of these contacts are preserved during Pol epsilon-dependent CMG assembly on path to origin firing, as observed with DNA replication reconstituted in vitr

Evidence against Equimolarity of Large Repeat Arrangements and a Predominant Master Circle Structure of the Mitochondrial Genome from a Monkeyflower (Mimulus guttatus) Lineage with Cryptic CMS

Despite intense investigation for over 25 years, the in vivo structure of plant mitochondrial genomes remains uncertain. Mapping studies and genome sequencing generally produce large circular chromosomes, whereas electrophoretic and microscopic studies typically reveal linear and multibranched molecules. To more fully assess the structure of plant mitochondrial genomes, the complete sequence of the monkeyflower (Mimulus guttatus DC. line IM62) mitochondrial DNA was constructed from a large (35 kb) paired-end shotgun sequencing library to a high depth of coverage (∼30×). The complete genome maps as a 525,671 bp circular molecule and exhibits a fairly conventional set of features including 62 genes (encoding 35 proteins, 24 transfer RNAs, and 3 ribosomal RNAs), 22 introns, 3 large repeats (2.7, 9.6, and 29 kb), and 96 small repeats (40–293 bp). Most paired-end reads (71%) mapped to the consensus sequence at the expected distance and orientation across the entire genome, validating the accuracy of assembly. Another 10% of reads provided clear evidence of alternative genomic conformations due to apparent rearrangements across large repeats. Quantitative assessment of these repeat-spanning read pairs revealed that all large repeat arrangements are present at appreciable frequencies in vivo, although not always in equimolar amounts. The observed stoichiometric differences for some arrangements are inconsistent with a predominant master circular structure for the mitochondrial genome of M. guttatus IM62. Finally, because IM62 contains a cryptic cytoplasmic male sterility (CMS) system, an in silico search for potential CMS genes was undertaken. The three chimeric open reading frames (ORFs) identified in this study, in addition to the previously identified ORFs upstream of the nad6 gene, are the most likely CMS candidate genes in this line