Dandyism as a lifestyle

Bakalářská práce se zabývá dandysmem jako specifickým životním stylem. Představuje teoretické vymezení dandysmu a zároveň jeho významné představitele v západní společnosti 19. století, jimiž byli George Brummell, George Gordon Byron, Jules-Amédée Barbey d'Aurevilly, Charles Baudelaire, Oscar Wilde, Robert de Montesquiou a Artur Breisky. Pozornost je věnována charakteristikám doby a společnosti, ve které se postava dandyho objevila. Dále se práce věnuje postavě dandyho z hlediska jeho hodnotové orientace, vzhledu, způsobu chování, zálib a trávení času. Práce se zaměřuje také na obraz dandysmu jako životního stylu v literatuře. Detailněji jsou představena zejména díla Fanfarlo, Obraz Doriana Graye a Naruby. V závěrečné části práce je diskutována aktuálnost dandysmu ve 20. a 21. století a jsou představeny tři osobnosti, jejichž životní styl s principy dandysmu rezonuje. Klíčová slova: dandysmus, dandy, životní styl, individualismus, hédonismus, estetismus, elegance, revoltaTheses focuses on dandyism as a lifestyle. It presents the theoretical definition of dandyism as well as its 19. century important representatives such as George Brummell, George Gordon Byron, Jules-Amédée Barbey d'Aurevilly, Charles Baudelaire, Oscar Wilde, Robert de Montesquiou and Artur Breisky. Attention is paid to the characteristics of the historical period and the society in which the dandy figure appeared. Theses also focuses on the dandy figure in terms of his value orientation, appearance, behaviors, interests and time spending. Moreover, theses deals with the image of dandyism as a lifestyle in literature. More detailed attention is paid to literary works Fanfarlo, The Picture of Dorian Gray and Against Nature. In the final part of the theses is discused the topicality of dandyism in 20th and 21st century and presented three personalities whose lifestyle resonates with the principles of dandyism. Key words: dandyism, dandy, lifestyle, individulism, aestheticism, hedonism, elegance, revoltInstitute of EthnologyÚstav etnologieFilozofická fakultaFaculty of Art

Differentiation-dependent glycosylation of cells in squamous cell epithelia detected by a mammalian lectin

The squamous stratified epithelia contain a proliferative (harboring mitotic activity) and a differentiating compartment. Due to the potential of protein-carbohyd rate interactions to regulate cellular activities we introduced a mammalian lectin to cyto- and histochemical analysis. We answer the questions of whether and to what extent this new probe can pinpoint differentiation-dependent glycosylation changes in sections and in culture of keratinocytes. Material and Methods: Purification and labeling enabled monitoring of galectin-3 reactivity in frozen sections of human and pig epidermis and basal cell carcinomas as well as in culture of keratinocytes. The staining pattern of the lectin was correlated with the staining profile of other cell markers including desmosomal proteins, beta(1) integrin, and the proliferation marker Ki-67. The Dolichos biflorus agglutinin (DBA) sharing binding reactivity of galectin-3 to the A type histoblood group epitope was used for comparison. Results: Both lectins exhibit suprabasal binding. However, their profiles were not identical, substantiated by lack of coinhibition. Strong DBA reactivity was also observed in a limited number of basal layer cells, namely in cells without the expression of the proliferation marker Ki-67. Cultured mitotic epidermal cells have no reactivity for DBA. Presence of ligands for this plant lectin was connected with decreased positivity of nuclei for Ki-67 and the occurrence of ring-shaped nucleoli, micronucleoli or absence of nucleoli. Considering colocalization the pattern of galectin-3-binding sites coincided with the presence of desmosomal proteins such as desmoplakin-1 and desmoglein but not beta(1) integrin, a potential ligand. Interestingly, studied basal cell carcinomas expressed no binding sites for galectin-3, while a limited number of cells were DBA-reactive. Conclusion: The expression of galectin-3-binding sites and also DBA-reactive glycoligands correlates with an increased level of differentiation and/or cessation of proliferation in the examined squamous stratified epithelia. Further application of tissue lectins for characterizing ligand expression and its modulation is an important step to reveal functional relevance

Differentiation-dependent glycosylation of cells in squamous cell epithelia detected by a mammalian lectin

The squamous stratified epithelia contain a proliferative (harboring mitotic activity) and a differentiating compartment. Due to the potential of protein-carbohyd rate interactions to regulate cellular activities we introduced a mammalian lectin to cyto- and histochemical analysis. We answer the questions of whether and to what extent this new probe can pinpoint differentiation-dependent glycosylation changes in sections and in culture of keratinocytes. Material and Methods: Purification and labeling enabled monitoring of galectin-3 reactivity in frozen sections of human and pig epidermis and basal cell carcinomas as well as in culture of keratinocytes. The staining pattern of the lectin was correlated with the staining profile of other cell markers including desmosomal proteins, beta(1) integrin, and the proliferation marker Ki-67. The Dolichos biflorus agglutinin (DBA) sharing binding reactivity of galectin-3 to the A type histoblood group epitope was used for comparison. Results: Both lectins exhibit suprabasal binding. However, their profiles were not identical, substantiated by lack of coinhibition. Strong DBA reactivity was also observed in a limited number of basal layer cells, namely in cells without the expression of the proliferation marker Ki-67. Cultured mitotic epidermal cells have no reactivity for DBA. Presence of ligands for this plant lectin was connected with decreased positivity of nuclei for Ki-67 and the occurrence of ring-shaped nucleoli, micronucleoli or absence of nucleoli. Considering colocalization the pattern of galectin-3-binding sites coincided with the presence of desmosomal proteins such as desmoplakin-1 and desmoglein but not beta(1) integrin, a potential ligand. Interestingly, studied basal cell carcinomas expressed no binding sites for galectin-3, while a limited number of cells were DBA-reactive. Conclusion: The expression of galectin-3-binding sites and also DBA-reactive glycoligands correlates with an increased level of differentiation and/or cessation of proliferation in the examined squamous stratified epithelia. Further application of tissue lectins for characterizing ligand expression and its modulation is an important step to reveal functional relevance

The Treat-to-Target Project in Atopic Dermatitis: One Year On

Atopic dermatitis is a chronic skin condition for which a range of systemic treatments have recently been approved. A treat-to-target strategy has been deve loped previously alongside an algorithm to guide the management of patients with atopic dermatitis. Here, we review the strategy and algorithm in the context of the evolving therapeutic landscape, and identify areas for further refinement and development

The Role of Museums in Adult Education

The theses is focused on the topic of museums and their role in adult education and learning. It provides a basic overview of the development of museums and their educational role in history. A special attention is also paid to adults as a specific target group of the museum education, their specifics in terms of the leisure education and the educational process. It is also devoted to the educational offer of museums in the field of formal and non-formal education and informal learning. A part of the thesis is a qualitative survey that focuses on the educational offer of selected museums and to what extent the specifics of adults are taken into account by the staff in the area of the museum education. Key words museum, museum education, adult, adut education, leisure education, non-formal education, informal learnin

Dandyism as a lifestyle

Theses focuses on dandyism as a lifestyle. It presents the theoretical definition of dandyism as well as its 19. century important representatives such as George Brummell, George Gordon Byron, Jules-Amédée Barbey d'Aurevilly, Charles Baudelaire, Oscar Wilde, Robert de Montesquiou and Artur Breisky. Attention is paid to the characteristics of the historical period and the society in which the dandy figure appeared. Theses also focuses on the dandy figure in terms of his value orientation, appearance, behaviors, interests and time spending. Moreover, theses deals with the image of dandyism as a lifestyle in literature. More detailed attention is paid to literary works Fanfarlo, The Picture of Dorian Gray and Against Nature. In the final part of the theses is discused the topicality of dandyism in 20th and 21st century and presented three personalities whose lifestyle resonates with the principles of dandyism. Key words: dandyism, dandy, lifestyle, individulism, aestheticism, hedonism, elegance, revol

Dandyism as a lifestyle

Theses focuses on dandyism as a lifestyle. It presents the theoretical definition of dandyism as well as its 19. century important representatives such as George Brummell, George Gordon Byron, Jules-Amédée Barbey d'Aurevilly, Charles Baudelaire, Oscar Wilde, Robert de Montesquiou and Artur Breisky. Attention is paid to the characteristics of the historical period and the society in which the dandy figure appeared. Theses also focuses on the dandy figure in terms of his value orientation, appearance, behaviors, interests and time spending. Moreover, theses deals with the image of dandyism as a lifestyle in literature. More detailed attention is paid to literary works Fanfarlo, The Picture of Dorian Gray and Against Nature. In the final part of the theses is discused the topicality of dandyism in 20th and 21st century and presented three personalities whose lifestyle resonates with the principles of dandyism. Key words: dandyism, dandy, lifestyle, individulism, aestheticism, hedonism, elegance, revol

Sinecatechins and imiquimod as proactive sequential therapy of external genital and perianal warts in adults

This review about the proactive sequential therapy (PST) of external genital and perianal warts (EGW) is based on the most current available clinical literature and on the broad clinical experience of a group of international experts, physicians who are well versed in the treatment of human papillomavirus-associated diseases. It provides a practical guide for the treatment of EGW, including epidemiology, etiology, clinical appearance, and diagnostic procedures for these viral infections. Furthermore, the treatment goals and current treatment options, elucidating provider- and patient-applied therapies, and the parameters driving treatment decisions are summarized. Specifically, the mode of action of the topical treatments sinecatechins and imiquimod, as well as the PST for EGW to achieve rapid and sustained clearance is discussed. The group of experts has developed a treatment algorithm giving healthcare providers a practical tool for the treatment of EGW which is very valuable in the presence of many different treatment options