The F-actin filament capping protein CapG is a bona fide nucleolar protein

Actin works in concert with myosin I to regulate the transcription of ribosomal genes in the nucleolus. Recently, nucleolar actin has been shown to be active in its polymeric form raising the question how actin dynamics is regulated in the nucleolus. Here, we show that the actin capping protein CapG localizes in the nucleolus of cultured cells. CapG transport to the nucleolus is an active and ATP-dependent process. Association of CapG with the nucleolus requires active RNA Polymerase I transcription. In addition, we show that activated Ran GTPase, an interaction partner of CapG, is also transported to the nucleolus. A constitutively active Ran mutant promotes CapG accumulation in the nucleolus indicating that CapG transport to the nucleolus can be supported by Ran. Our results suggest that filamentous actin in the nucleolus might be regulated by actin binding proteins such as CapG. (C

Post-breakage behaviour of laminated glass in structural applications

Firstly is introduced what the post-breakage behaviour of laminated safety glass is, and why it's important to can model it for designing structural glass elements. A general description of post-breakage behaviour and the different possible mechanisms leading to failure are presented. We then focus on the mechanical properties of the interlayer material, more specifically in the perspective to model its behaviour at large strain up to break. Typical results of standard uniaxial tensile tests on SGP samples (SentryGlas (R) Plus, interlayer of DuPont de Nemours) are shortly presented, and we then explain why those are insufficient to calibrate numeric material models to use in finite elements softwares. Finally perspectives for further experimental investigation with aiming to calibrate material models are presented

A story about standardization for design of glass works

This contribution attempts to give an overview of the European standardization framework which is related to the design and calculation of glass works in buildings. The different work levels, institutions, technical committees and workgroups, and the various types of documents and their statute will be introduced, explaining the difference between draft, experimental and final standards, between harmonized, support and design standards, the general and particular meaning of harmonization and implementation. The standardization framework is explained firstly from the point of view of European standardization policy and history, and secondly regarding the standardization framework in Belgium. In particular, an attempt is made to highlight some particularities existing in harmonization efforts of design methods and codes for glass works

Failure behaviour of glass laminates with a "stiff" interlayer at room temperature

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