Improved hydrogen gas production in microbial electrolysis cells using inexpensive recycled carbon fibre fabrics

Growing energy demands of wastewater treatment have made it vital for water companies to develop less energy intensive processes for treating wastewater if net zero emissions are to be achieved by 2050. Microbial electrolysis cells (MECs) have the potential to do this by treating water and producing renewable hydrogen gas as a product, but capital and operational costs have slowed their deployment. By using recycled carbon fibre mats, commercially viable MECs can brought closer to reality, where recycled carbon fibre anode MECs treating real wastewater (normalised ~3100 L d−1) were producing 66.77 L H2 d−1 while graphite felt anode MECs produced 3.65 L H2 d−1 per 1 m3 reactor, anodes costing £5.53 m−2 and £88.36 m−2 respectively, resulting in a total anode cost saving of 93%. This could incentivise the development of larger pilot systems, opening the door for generating greater value and a more sustainable wastewater treatment industry

Distribution of Protein I, a Synapse-Specific Phosphoprotein, and Adenylate Cyclase in the Rat Spinal Cord

The longitudinal and transverse distributions of the synapse-specific phosphoprotein Protein I and adenylate cyclase in the rat spinal cord were studied. Protein I was found to be enriched in all cervical and midlumbar (L 3 -L 5 ) segments, and sparse in midthoracic and sacral segments. Adenylate cyclase activity was high in all cervical and lumbosacral segments, and low in mid-thoracic segments. Cross-sectionally, both Protein I and adenylate cyclase were more enriched in the dorsal half than in the ventral half in the various segments studied. The similar topographical distributions of Protein 1 and adenylate cyclase in the spinal cord support the idea that adenylate cyclase may be intimately associated with Protein I in the nervous system, and could thereby regulate the state of in vivo phosphorylation of Protein I through formation of cyclic AMP.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66108/1/j.1471-4159.1981.tb02407.x.pd

Higher education and the Anthropocene - Towards an ecological approach to higher education policy in New Zealand

In this thesis I present an ecological direction for higher education policy in Aotearoa/New Zealand. This position is developed through an ecological approach to policy, which includes a postfoundational take on ecological theory, especially the work of Gregory Bateson and Felix Guattari. This ecological approach to higher education policy is in contrast to the neoliberal and technicist policy thinking which has informed New Zealand’s Tertiary Education Strategy (Ministry of Education, 2014c). As a contrast, the ecological approach in this thesis draws strength from ecological economics, environmental politics, critical policy analysis, ecological theory and philosophical pragmatism. The methodological core of this approach is described as Critical Eco Pragmatism (CEP). Following a discussion of ecological theory and an exploration of the Global Ecological Crisis (GEC) as an interconnected problem of natural, political, social, psychological, pedagogical and epistemological dimensions, I develop a theoretical framework for being ecological in higher education. This framework draws on a critique of Ron Barnett’s work on the ecological university (Barnett, 2010, 2018) and introduces the notion of ‘Anthropocene Intelligence’. Anthropocene Intelligence provides a way to pragmatically bring together a range of theoretical ideas about education – especially those ideas that have a claim on improving our psychological, social and natural ecologies. This includes educational discourses that have not always had a high level of interaction, such as environmental and sustainability education (ESE), indigenous education, eco-pedagogy, engaged scholarship, ecological humanities, human development education, and education for wellbeing (including the healthy university). The potential of an ecological approach is also considered in relation to the many practical possibilities that currently exist in higher education policy and practice both internationally and in New Zealand. Together with the theoretical approach taken in this thesis, these practical possibilities inform the alternative, ecological direction this thesis develops for higher education policy in New Zealand. Included in this ecological direction is the aspiration for New Zealand to develop as an ‘ecological democracy’ (Dryzek, 2013)

Wetland hydrological monitoring: overview and Boxford Water Meadows case study

The aim of this report is to provide the reader with the information required to make informed decisions about the best and most appropriate way to monitor a wetland site. To achieve this aim, the report has the following objectives: 1. To outline the need and purpose for monitoring. 2. To summarise the methods used to identify and categorise wetland types. 3. To describe the broad types of monitoring that may be undertaken. 4. To give detailed information about the range of wetland monitoring techniques available. 5. To provide guidance on how to select the most appropriate monitoring techniques. 6. To illustrate, using the Boxford wetland as a case study, how the techniques described in this report can be applied, and what challenges and solutions are encountered

Electrically induced bacterial membrane-potential dynamics correspond to cellular proliferation capacity

Membrane-potential dynamics mediate bacterial electrical signaling at both intra- and intercellular levels. Membrane potential is also central to cellular proliferation. It is unclear whether the cellular response to external electrical stimuli is influenced by the cellular proliferative capacity. A new strategy enabling electrical stimulation of bacteria with simultaneous monitoring of single-cell membrane- potential dynamics would allow bridging this knowledge gap and further extend electrophysiological studies into the field of microbi- ology. Here we report that an identical electrical stimulus can cause opposite polarization dynamics depending on cellular proliferation capacity. This was demonstrated using two model organisms, namely Bacillus subtilis and Escherichia coli, and by developing an apparatus enabling exogenous electrical stimulation and single-cell time-lapse microscopy. Using this bespoke apparatus, we show that a 2.5-sec- ond electrical stimulation causes hyperpolarization in unperturbed cells. Measurements of intracellular K+ and the deletion of the K+ channel suggested that the hyperpolarization response is caused by the K+ efflux through the channel. When cells are preexposed to 400 ± 8 nm wavelength light, the same electrical stimulation depolarizes cells instead of causing hyperpolarization. A mathematical model extended from the FitzHugh–Nagumo neuron model suggested that the opposite response dynamics are due to the shift in resting mem- brane potential. As predicted by the model, electrical stimulation only induced depolarization when cells are treated with antibiotics, protonophore, or alcohol. Therefore, electrically induced membrane- potential dynamics offer a reliable approach for rapid detection of proliferative bacteria and determination of their sensitivity to anti- microbial agents at the single-cell level

The mechanisms and processes of connection: developing a causal chain model capturing impacts of receiving recorded mental health recovery narratives.

BACKGROUND: Mental health recovery narratives are a core component of recovery-oriented interventions such as peer support and anti-stigma campaigns. A substantial number of recorded recovery narratives are now publicly available online in different modalities and in published books. Whilst the benefits of telling one's story have been investigated, much less is known about how recorded narratives of differing modalities impact on recipients. A previous qualitative study identified connection to the narrator and/or to events in the narrative to be a core mechanism of change. The factors that influence how individuals connect with a recorded narrative are unknown. The aim of the current study was to characterise the immediate effects of receiving recovery narratives presented in a range of modalities (text, video and audio), by establishing the mechanisms of connection and the processes by which connection leads to outcomes. METHOD: A study involving 40 mental health service users in England was conducted. Participants were presented with up to 10 randomly-selected recovery narratives and were interviewed on the immediate impact of each narrative. Thematic analysis was used to identify the mechanisms of connection and how connection leads to outcome. RESULTS: Receiving a recovery narrative led participants to reflect upon their own experiences or those of others, which then led to connection through three mechanisms: comparing oneself with the narrative and narrator; learning about other's experiences; and experiencing empathy. These mechanisms led to outcomes through three processes: the identification of change (through attending to narrative structure); the interpretation of change (through attending to narrative content); and the internalisation of interpretations. CONCLUSIONS: This is the first study to identify mechanisms and processes of connection with recorded recovery narratives. The empirically-based causal chain model developed in this study describes the immediate effects on recipients. This model can inform selection of narratives for use in interventions, and be used to support peer support workers in recounting their own recovery narratives in ways which are maximally beneficial to others

Mechanisms of apoptosis sensitivity and resistance to the BH3 mimetic ABT-737 in acute myeloid leukemia

SummaryBCL-2 proteins are critical for cell survival and are overexpressed in many tumors. ABT-737 is a small-molecule BH3 mimetic that exhibits single-agent activity against lymphoma and small-cell lung cancer in preclinical studies. We here report that ABT-737 effectively kills acute myeloid leukemia blast, progenitor, and stem cells without affecting normal hematopoietic cells. ABT-737 induced the disruption of the BCL-2/BAX complex and BAK-dependent but BIM-independent activation of the intrinsic apoptotic pathway. In cells with phosphorylated BCL-2 or increased MCL-1, ABT-737 was inactive. Inhibition of BCL-2 phosphorylation and reduction of MCL-1 expression restored sensitivity to ABT-737. These data suggest that ABT-737 could be a highly effective antileukemia agent when the mechanisms of resistance identified here are considered