664 research outputs found
Primary accumulation in the Soviet transition
The Soviet background to the idea of primary socialist accumulation is presented. The mobilisation of labour power and of products into public sector investment from outside are shown to have been the two original forms of the concept. In Soviet primary accumulation the mobilisation of labour power was apparently more decisive than the mobilisation of products. The primary accumulation process had both intended and unintended results. Intended results included bringing most of the economy into the public sector, and industrialisation of the economy as a whole. Unintended results included substantial economic losses, and the proliferation of coercive institutions damaging to attainment of the ultimate goal - the building of a communist society
Mitochondrial and chloroplast stress responses are modulated in distinct touch and chemical inhibition phases
Previous studies have identified a range of transcription factors that modulate retrograde regulation of mitochondrial and chloroplast functions in Arabidopsis (Arabidopsis thaliana). However, the relative importance of these regulators and whether they act downstream of separate or overlapping signaling cascades is still unclear. Here, we demonstrate that multiple stress-related signaling pathways, with distinct kinetic signatures, converge on overlapping gene sets involved in energy organelle function. The transcription factor ANAC017 is almost solely responsible for transcript induction of marker genes around 3 to 6 h after chemical inhibition of organelle function and is a key regulator of mitochondrial and specific types of chloroplast retrograde signaling. However, an independent and highly transient gene expression phase, initiated within 10 to 30 min after treatment, also targets energy organelle functions, and is related to touch and wounding responses. Metabolite analysis demonstrates that this early response is concurrent with rapid changes in tricarboxylic acid cycle intermediates and large changes in transcript abundance of genes encoding mitochondrial dicarboxylate carrier proteins. It was further demonstrated that transcription factors AtWRKY15 and AtWRKY40 have repressive regulatory roles in this touch-responsive gene expression. Together, our results show that several regulatory systems can independently affect energy organelle function in response to stress, providing different means to exert operational control
FLOWERING LOCUS C -dependent and -independent regulation of the circadian clock by the autonomous and vernalization pathways
Background
The circadian system drives pervasive biological rhythms in plants. Circadian clocks integrate endogenous timing information with environmental signals, in order to match rhythmic outputs to the local day/night cycle. Multiple signaling pathways affect the circadian system, in ways that are likely to be adaptively significant. Our previous studies of natural genetic variation in Arabidopsis thaliana accessions implicated FLOWERING LOCUS C (FLC) as a circadian-clock regulator. The MADS-box transcription factor FLC is best known as a regulator of flowering time. Its activity is regulated by many regulatory genes in the "autonomous" and vernalization-dependent flowering pathways. We tested whether these same pathways affect the circadian system.
Results
Genes in the autonomous flowering pathway, including FLC, were found to regulate circadian period in Arabidopsis. The mechanisms involved are similar, but not identical, to the control of flowering time. By mutant analyses, we demonstrate a graded effect of FLC expression upon circadian period. Related MADS-box genes had less effect on clock function. We also reveal an unexpected vernalization-dependent alteration of periodicity.
Conclusion
This study has aided in the understanding of FLC's role in the clock, as it reveals that the network affecting circadian timing is partially overlapping with the floral-regulatory network. We also show a link between vernalization and circadian period. This finding may be of ecological relevance for developmental programing in other plant species
The Aspergillus nidulans cnxABC locus is a single gene encoding two catalytic domains required for synthesis of precursor Z, an intermediate in molybdenum cofactor biosynthesis
Vestiges of the proto-Caribbean seaway: origin of the San Souci Volcanic Group, Trinidad
Outcrops of volcanic–hypabyssal rocks in Trinidad document the opening of the proto-Caribbean seaway during Jurassic–Cretaceous break-up of the Americas. The San Souci Group on the northern coast of Trinidad comprises the San Souci Volcanic Formation (SSVF) and passive margin sediments of the ~ 130–125 Ma Toco Formation. The Group was trapped at the leading edge of the Pacific-derived Caribbean Plate during the Cretaceous–Palaeogene, colliding with the para-autochthonous margin of Trinidad during the Oligocene–Miocene. In-situ U–Pb ion probe dating of micro-zircons from a mafic volcanic breccia reveal the SSVF crystallised at 135.0 ± 7.3 Ma. The age of the SSVF is within error of the age of the Toco Formation. Assuming a conformable contact, geodynamic models indicate a likely origin for the SSVF on the passive margin close to the northern tip of South America. Immobile element and Nd–Hf radiogenic isotope signatures of the mafic rocks indicate the SSVF was formed by ≪10% partial melting of a heterogeneous spinel peridotite source with no subduction or continental lithospheric mantle component. Felsic breccias within the SSVF are more enriched in incompatible elements, with isotope signatures that are less radiogenic than the mafic rocks of the SSVF. The felsic rocks may be derived from re-melting of mafic crust. Although geochemical comparisons are drawn here with proto-Caribbean igneous outcrops in Venezuela and elsewhere in the Caribbean more work is needed to elucidate the development of the proto-Caribbean seaway and its rifted margins. In particular, ion probe dating of micro-zircons may yield valuable insights into magmatism and metamorphism in the Caribbean, and in altered basaltic terranes more generally
Gemini Planet Imager Observational Calibrations VI: Photometric and Spectroscopic Calibration for the Integral Field Spectrograph
The Gemini Planet Imager (GPI) is a new facility instrument for the Gemini
Observatory designed to provide direct detection and characterization of
planets and debris disks around stars in the solar neighborhood. In addition to
its extreme adaptive optics and corona graphic systems which give access to
high angular resolution and high-contrast imaging capabilities, GPI contains an
integral field spectrograph providing low resolution spectroscopy across five
bands between 0.95 and 2.5 m. This paper describes the sequence of
processing steps required for the spectro-photometric calibration of GPI
science data, and the necessary calibration files. Based on calibration
observations of the white dwarf HD 8049B we estimate that the systematic error
in spectra extracted from GPI observations is less than 5%. The flux ratio of
the occulted star and fiducial satellite spots within coronagraphic GPI
observations, required to estimate the magnitude difference between a target
and any resolved companions, was measured in the -band to be in laboratory measurements and using
on-sky observations. Laboratory measurements for the , , and
filters are also presented. The total throughput of GPI, Gemini South and the
atmosphere of the Earth was also measured in each photometric passband, with a
typical throughput in -band of 18% in the non-coronagraphic mode, with some
variation observed over the six-month period for which observations were
available. We also report ongoing development and improvement of the data cube
extraction algorithm.Comment: 15 pages, 6 figures. Proceedings of the SPIE, 9147-30
Negative regulation of syntaxin4/SNAP-23/VAMP2-mediated membrane fusion by Munc18c <i>In Vitro</i>
Background: Translocation of the facilitative glucose transporter GLUT4 from an intracellular store to the plasma membrane is responsible for the increased rate of glucose transport into fat and muscle cells in response to insulin. This represents a specialised form of regulated membrane trafficking. Intracellular membrane traffic is subject to multiple levels of regulation by conserved families of proteins in all eukaryotic cells. Notably, all intracellular fusion events require SNARE proteins and Sec1p/Munc18 family members. Fusion of GLUT4-containing vesicles with the plasma membrane of insulin-sensitive cells involves the SM protein Munc18c, and is regulated by the formation of syntaxin 4/SNAP23/VAMP2 SNARE complexes. Methodology/Principal Findings Here we have used biochemical approaches to characterise the interaction(s) of Munc18c with its cognate SNARE proteins and to examine the role of Munc18c in regulating liposome fusion catalysed by syntaxin 4/SNAP23/VAMP2 SNARE complex formation. We demonstrate that Munc18c makes contacts with both t- and v-SNARE proteins of this complex, and directly inhibits bilayer fusion mediated by the syntaxin 4/SNAP23/VAMP2 SNARE complex. Conclusion/Significance Our reductionist approach has enabled us to ascertain a direct inhibitory role for Munc18c in regulating membrane fusion mediated by syntaxin 4/SNAP23/VAMP2 SNARE complex formation. It is important to note that two different SM proteins have recently been shown to stimulate liposome fusion mediated by their cognate SNARE complexes. Given the structural similarities between SM proteins, it seems unlikely that different members of this family perform opposing regulatory functions. Hence, our findings indicate that Munc18c requires a further level of regulation in order to stimulate SNARE-mediated membrane fusion
Bringing "The Moth" to Light: A Planet-Sculpting Scenario for the HD 61005 Debris Disk
The HD 61005 debris disk ("The Moth") stands out from the growing collection
of spatially resolved circumstellar disks by virtue of its unusual swept-back
morphology, brightness asymmetries, and dust ring offset. Despite several
suggestions for the physical mechanisms creating these features, no definitive
answer has been found. In this work, we demonstrate the plausibility of a
scenario in which the disk material is shaped dynamically by an eccentric,
inclined planet. We present new Keck NIRC2 scattered-light angular differential
imaging of the disk at 1.2-2.3 microns that further constrains its outer
morphology (projected separations of 27-135 AU). We also present complementary
Gemini Planet Imager 1.6 micron total intensity and polarized light detections
that probe down to projected separations less than 10 AU. To test our
planet-sculpting hypothesis, we employed secular perturbation theory to
construct parent body and dust distributions that informed scattered-light
models. We found that this method produced models with morphological and
photometric features similar to those seen in the data, supporting the premise
of a planet-perturbed disk. Briefly, our results indicate a disk parent body
population with a semimajor axis of 40-52 AU and an interior planet with an
eccentricity of at least 0.2. Many permutations of planet mass and semimajor
axis are allowed, ranging from an Earth mass at 35 AU to a Jupiter mass at 5
AU.Comment: Accepted to AJ; added Figure 5 and minor text edit
Structural Causes of Right Bundle Branch Block—Time for a Closer Look?
Right bundle branch block is an electrocardiographic phenomenon with specific criteria
- …