The Minimal Moose for a Little Higgs

Recently a new class of theories of electroweak symmetry breaking have been constructed. These models, based on deconstruction and the physics of theory space, provide the first alternative to weak-scale supersymmetry with naturally light Higgs fields and perturbative new physics at the TeV scale. The Higgs is light because it is a pseudo-Goldstone boson, and the quadratically divergent contributions to the Higgs mass are cancelled by new TeV scale ``partners'' of the {\em same} statistics. In this paper we present the minimal theory space model of electroweak symmetry breaking, with two sites and four link fields, and the minimal set of fermions. There are very few parameters and degrees of freedom beyond the Standard Model. Below a TeV, we have the Standard Model with two light Higgs doublets, and an additional complex scalar weak triplet and singlet. At the TeV scale, the new particles that cancel the 1-loop quadratic divergences in the Higgs mass are revealed. The entire Higgs potential needed for electroweak symmetry breaking--the quartic couplings as well as the familiar negative mass squared--can be generated by the top Yukawa coupling, providing a novel link between the physics of flavor and electroweak symmetry breaking.Comment: 15 pages. References added. Included clarifying comments on the origin of quartic couplings, and on power-counting. More elegant model for generating Higgs potential from top Yukawa coupling presente

A Phenomenological Description of (\pi^{-}\Delta^{++}) Photo- and Electroproduction in Nucleon Resonance Region

The (\pi^{-}\Delta^{++}) production on the nucleon by real and virtual photons is discussed as initial step in a simple model approach for the two pion photo- and electroproduction on the nucleon, with emphasis on nucleon resonance excitation which is of interest for new facilities like TJNAF. A calculation for (\pi^{-}\Delta^{++}) channel in resonance excitation region is presented and compared to existing experimental data along with a discussion of physical effects that we find to be of relevance. The calculation is proposed as a starting basis for the investigation of (N^{*}) electromagnetic form factors using experimental data about two pion production by real and virtual photons.Comment: 36 pages, 14 figures, to be published in Nucl. Phys.

Flow cytometry as a rapid analytical tool to determine physiological responses to changing O2 and iron concentration by Magnetospirillum gryphiswaldense strain MSR-1

Magnetotactic bacteria (MTB) are a diverse group of bacteria that synthesise magnetosomes, magnetic membrane-bound nanoparticles that have a variety of diagnostic, clinical and biotechnological applications. We present the development of rapid methods using flow cytometry to characterize several aspects of the physiology of the commonly-used MTB Magnetospirillum gryphiswaldense MSR-1. Flow cytometry is an optical technique that rapidly measures characteristics of individual bacteria within a culture, thereby allowing determination of population heterogeneity and also permitting direct analysis of bacteria. Scatter measurements were used to measure and compare bacterial size, shape and morphology. Membrane permeability and polarization were measured using the dyes propidium iodide and bis-(1,3-dibutylbarbituric acid) trimethine oxonol to determine the viability and ‘health’ of bacteria. Dyes were also used to determine changes in concentration of intracellular free iron and polyhydroxylakanoate (PHA), a bacterial energy storage polymer. These tools were then used to characterize the responses of MTB to different O2 concentrations and iron-sufficient or iron-limited growth. Rapid analysis of MTB physiology will allow development of bioprocesses for the production of magnetosomes, and will increase understanding of this fascinating and useful group of bacteria

Molecular Determinants and Dynamics of Hepatitis C Virus Secretion

The current model of hepatitis C virus (HCV) production involves the assembly of virions on or near the surface of lipid droplets, envelopment at the ER in association with components of VLDL synthesis, and egress via the secretory pathway. However, the cellular requirements for and a mechanistic understanding of HCV secretion are incomplete at best. We combined an RNA interference (RNAi) analysis of host factors for infectious HCV secretion with the development of live cell imaging of HCV core trafficking to gain a detailed understanding of HCV egress. RNAi studies identified multiple components of the secretory pathway, including ER to Golgi trafficking, lipid and protein kinases that regulate budding from the trans-Golgi network (TGN), VAMP1 vesicles and adaptor proteins, and the recycling endosome. Our results support a model wherein HCV is infectious upon envelopment at the ER and exits the cell via the secretory pathway. We next constructed infectious HCV with a tetracysteine (TC) tag insertion in core (TC-core) to monitor the dynamics of HCV core trafficking in association with its cellular cofactors. In order to isolate core protein movements associated with infectious HCV secretion, only trafficking events that required the essential HCV assembly factor NS2 were quantified. TC-core traffics to the cell periphery along microtubules and this movement can be inhibited by nocodazole. Sub-populations of TC-core localize to the Golgi and co-traffic with components of the recycling endosome. Silencing of the recycling endosome component Rab11a results in the accumulation of HCV core at the Golgi. The majority of dynamic core traffics in association with apolipoprotein E (ApoE) and VAMP1 vesicles. This study identifies many new host cofactors of HCV egress, while presenting dynamic studies of HCV core trafficking in infected cells