Acoustic signalling for mate attraction in crickets: Abdominal ganglia control the timing of the calling song pattern.

Decoding the neural basis of behaviour requires analysing how the nervous system is organised and how the temporal structure of motor patterns emerges from its activity. The stereotypical patterns of the calling song behaviour of male crickets, which consists of chirps and pulses, is an ideal model to study this question. We applied selective lesions to the abdominal nervous system of field crickets and performed long-term acoustic recordings of the songs. Specific lesions to connectives or ganglia abolish singing or reliably alter the temporal features of the chirps and pulses. Singing motor control appears to be organised in a modular and hierarchically fashion, where more posterior ganglia control the timing of the chirp pattern and structure and anterior ganglia the timing of the pulses. This modular organisation may provide the substrate for song variants underlying calling, courtship and rivalry behaviour and for the species-specific song patterns in extant crickets.PFJ was supported by the Fundação para a Ciência e a Tecnologia, Portugal (SFRH/BD/51901/2012) and the equipment used in this research was funded by the BBSRC (BB/G018723/1).This is the final version of the article. It first appeared from Elsevier via https://doi.org/10.1016/j.bbr.2016.04.02

Structure, Activity and Function of a Singing CPG Interneuron Controlling Cricket Species-Specific Acoustic Signaling.

The evolution of species-specific song patterns is a driving force in the speciation of acoustic communicating insects. It must be closely linked to adaptations of the neuronal network controlling the underlying singing motor activity. What are the cellular and network properties that allow generating different songs? In five cricket species, we analyzed the structure and activity of the identified abdominal ascending opener interneuron, a homologous key component of the singing central pattern generator. The structure of the interneuron, based on the position of the cell body, ascending axon, dendritic arborization pattern, and dye coupling, is highly similar across species. The neuron's spike activity shows a tight coupling to the singing motor activity. In all species, current injection into the interneuron drives artificial song patterns, highlighting the key functional role of this neuron. However, the pattern of the membrane depolarization during singing, the fine dendritic and axonal ramifications, and the number of dye-coupled neurons indicate species-specific adaptations of the neuronal network that might be closely linked to the evolution of species-specific singing.SIGNIFICANCE STATEMENT A fundamental question in evolutionary neuroscience is how species-specific behaviors arise in closely related species. We demonstrate behavioral, neurophysiological, and morphological evidence for homology of one key identified interneuron of the singing central pattern generator in five cricket species. Across-species differences of this interneuron are also observed, which might be important to the generation of the species-specific song patterns. This work offers a comprehensive and detailed comparative analysis addressing the neuronal basis of species-specific behavior

Modular timer networks: abdominal interneurons controlling the chirp and pulse pattern in a cricket calling song

Funder: University of CambridgeAbstract: Chirping male crickets combine a 30 Hz pulse pattern with a 3 Hz chirp pattern to drive the rhythmic opening-closing movements of the front wings for sound production. Lesion experiments suggest two coupled modular timer-networks located along the chain of abdominal ganglia, a network in A3 and A4 generating the pulse pattern, and a network organized along with ganglia A4–A6 controlling the generation of the chirp rhythm. We analyzed neurons of the timer-networks and their synaptic connections by intracellular recordings and staining. We identified neurons spiking in phase with the chirps and pulses, or that are inhibited during the chirps. Neurons share a similar “gestalt”, regarding the position of the cell body, the dendritic arborizations and the contralateral ascending axon. Activating neurons of the pulse-timer network elicits ongoing motor activity driving the generation of pulses; this activity is not structured in the chirp pattern. Activating neurons of the chirp-timer network excites pulse-timer neurons; it drives the generation of chirps and during the chirps the pulse pattern is produced. Our results support the hypothesis that two modular networks along the abdominal ganglion chain control the cricket calling song, a pattern generating network in the mesothoracic ganglion may not be required

Multisensory learning binds neurons into a cross-modal memory engram

Associating multiple sensory cues with objects and experience is a fundamental brain process that improves object recognition and memory performance. However, neural mechanisms that bind sensory features during learning and augment memory expression are unknown. Here we demonstrate multisensory appetitive and aversive memory in Drosophila. Combining colours and odours improved memory performance, even when each sensory modality was tested alone. Temporal control of neuronal function revealed visually selective mushroom body Kenyon cells (KCs) to be required for enhancement of both visual and olfactory memory after multisensory training. Voltage imaging in head-fixed flies showed that multisensory learning binds activity between streams of modality-specific KCs so that unimodal sensory input generates a multimodal neuronal response. Binding occurs between regions of the olfactory and visual KC axons, which receive valence-relevant dopaminergic reinforcement, and is propagated downstream. Dopamine locally releases GABAergic inhibition to permit specific microcircuits within KC-spanning serotonergic neurons to function as an excitatory bridge between the previously ‘modality-selective’ KC streams. Cross-modal binding thereby expands the KCs representing the memory engram for each modality into those representing the other. This broadening of the engram improves memory performance after multisensory learning and permits a single sensory feature to retrieve the memory of the multimodal experience

MLL5 improves ATRA driven differentiation and promotes xenotransplant engraftment in acute promyelocytic leukemia model

Although the mixed lineage leukemia 5 (MLL5) gene has prognostic implications in acute promyelocyte leukemia (APL), the underlying mechanism remains to be elucidated. Here, we demonstrate the critical role exerted by MLL5 in APL regarding cell proliferation and resistance to drug-induced apoptosis, through mtROS regulation. Additionally, MLL5 overexpression increased the responsiveness of APL leukemic cells to all-trans retinoic acid (ATRA)-induced differentiation, via regulation of the epigenetic modifiers SETD7 and LSD1. In silico analysis indicated that APL blasts with MLL5(high) transcript levels were associated with retinoic acid binding and downstream signaling, while MLL5(low) blasts displayed decreased expression of epigenetic modifiers (such as KMT2C, PHF8 and ARID4A). Finally, APL xenograft transplants demonstrated improved engraftment of MLL5-expressing cells and increased myeloid differentiation over time. Concordantly, evaluation of engrafted blasts revealed increased responsiveness of MLL5-expressing cells to ATRA-induced granulocytic differentiation. Together, we describe the epigenetic changes triggered by the interaction of MLL5 and ATRA resulting in enhanced granulocytic differentiation

Integration of Parallel Opposing Memories Underlies Memory Extinction.

Accurately predicting an outcome requires that animals learn supporting and conflicting evidence from sequential experience. In mammals and invertebrates, learned fear responses can be suppressed by experiencing predictive cues without punishment, a process called memory extinction. Here, we show that extinction of aversive memories in Drosophila requires specific dopaminergic neurons, which indicate that omission of punishment is remembered as a positive experience. Functional imaging revealed co-existence of intracellular calcium traces in different places in the mushroom body output neuron network for both the original aversive memory and a new appetitive extinction memory. Light and ultrastructural anatomy are consistent with parallel competing memories being combined within mushroom body output neurons that direct avoidance. Indeed, extinction-evoked plasticity in a pair of these neurons neutralizes the potentiated odor response imposed in the network by aversive learning. Therefore, flies track the accuracy of learned expectations by accumulating and integrating memories of conflicting events.S.W. was funded by a Wellcome Principal Research Fellowship (200846/Z/16/Z), by the Gatsby Charitable Foundation (GAT3237), and by the Bettencourt-Schueller Foundation. J.F. was supported by the DFG (FE 1563/1-1). G.S.X.E.J. was funded by Medical Research Council. D.D.B. funded by HHMI. G.S.X.E.J., D.D.B., and S.W. were funded by a Wellcome Collaborative Award (203261/Z/16/Z)

Influência da calagem e micronutrientes na nodulação da soja por Rhizobium japonicum em solos ácidos

Three experiments were performed in field conditions and in the glasshouse. Four different levels of lime and micronutrients were applied and the effect of inoculation with various strains of R. japonicum on the yield and symbiotic performance of soybean was studied. In the first experiment the Rhizobium inoculant used was prepared from a suspension of macerated nodules. In the other two experiments various inoculants were used. The effect of the higher rates of lime addition (4 and 6 t/ha) was to reduce dry weight of nodules, but did not affect plant dry weight and total plant nitrogen. The results show that an addition of 4 t/ha of lime is sufficient for maximum yield in the experimental conditions. There was a significant positive effect of zinc additions. An application of 50 kg FTE Br 12 supplied sufficient of this element. The different inoculants prepared, showed the same efficiency in the field conditions.Em três experimentos conduzidos em campo e em casa de vegetação, foram avaliados os efeitos de quatro níveis de calcário e diferentes estirpes de Rhizobium japonicum no rendimento de grãos e simbiose da soja. No primeiro experimento foi utilizado inoculante preparado a partir de suspensão de nódulos macerados e nos demais, vários inoculantes. Nos níveis mais elevados de calcário (4 e 6 t/ha), houve uma redução no peso seco de nódulos sem influenciar o peso seco da parte aérea e nitrogênio total acumulado nas plantas até o enchimento de grão. Os resultados demonstram ter sido suficiente a utilização de 4 t/ha de calcário, em termos de produção de grãos, para as condições experimentais. Houve efeito do zinco na nodulação e produção de grãos, sendo que o uso de 50 kg/ha de FTE Br-12 supriu plenamente a necessidade deste elemento. Os diferentes inoculantes utilizados apresentaram a mesma eficiência em campo

Feature selection in the reconstruction of complex network representations of spectral data

Complex networks have been extensively used in the last decade to characterize and analyze complex systems, and they have been recently proposed as a novel instrument for the analysis of spectra extracted from biological samples. Yet, the high number of measurements composing spectra, and the consequent high computational cost, make a direct network analysis unfeasible. We here present a comparative analysis of three customary feature selection algorithms, including the binning of spectral data and the use of information theory metrics. Such algorithms are compared by assessing the score obtained in a classification task, where healthy subjects and people suffering from different types of cancers should be discriminated. Results indicate that a feature selection strategy based on Mutual Information outperforms the more classical data binning, while allowing a reduction of the dimensionality of the data set in two orders of magnitud

Measurement of the cosmic ray spectrum above 4×10184{\times}10^{18} eV using inclined events detected with the Pierre Auger Observatory

A measurement of the cosmic-ray spectrum for energies exceeding $4{\times}10^{18}$ eV is presented, which is based on the analysis of showers with zenith angles greater than $60^{\circ}$ detected with the Pierre Auger Observatory between 1 January 2004 and 31 December 2013. The measured spectrum confirms a flux suppression at the highest energies. Above $5.3{\times}10^{18}$ eV, the "ankle", the flux can be described by a power law $E^{-\gamma}$ with index $\gamma=2.70 \pm 0.02 \,\text{(stat)} \pm 0.1\,\text{(sys)}$ followed by a smooth suppression region. For the energy ($E_\text{s}$) at which the spectral flux has fallen to one-half of its extrapolated value in the absence of suppression, we find $E_\text{s}=(5.12\pm0.25\,\text{(stat)}^{+1.0}_{-1.2}\,\text{(sys)}){\times}10^{19}$ eV.Comment: Replaced with published version. Added journal reference and DO