Evaluation of antibacterial and antifungal compounds for selective inhibition of denitrification in soils

Nitrous oxide (N2O) is an atmospheric constituent implicated in climate warming and stratospheric ozone depletion. Both bacteria and fungi participate in N2O production, but information is lacking with regard to the relative contribution of bacterial and fungal denitrifiers to the denitrification process in agricultural soils. The selective inhibition (SI) technique is widely used to assess the contribution of different groups of microbes to soil processes, but success of the technique depends on the effectiveness of the inhibitors. In this study, laboratory experiments were conducted to assess the contribution of bacteria and fungi to denitrification using soils from a woodlot, agricultural fields under conventional plowing (PT), and no-till for either 50 years (long-term) or 11 years (medium-term). A selective inhibition (SI) technique was developed using two bactericides (streptomycin and bronopol) and two fungicides (cycloheximide and captan) applied at different rates (0–32 mg per g soil). Regardless of the application rate, streptomycin and cycloheximide were not effective inhibitors of denitrification, with a degree of inhibition only between 2 and 20% relative to controls. These results are significant given the wide use of these products in SI studies. However, the bactericide bronopol and the fungicide captan effectively inhibited denitrification, with the strongest inhibition observed at an application rate of 16 mg per g soil. The ratio of fungal to bacterial denitrification activity (F : B) was generally less than 1, indicating a dominance of bacteria in denitrification activity in the soils investigated. However, an increase in the F : B ratio from 0.24 in medium-term NT to 0.87 in long-term NT soils was noted, suggesting perhaps a progressive increase in the role of fungal denitrifiers with a longer duration of NT farming

Hydro-geomorphic controls of greenhouse gas fluxes in riparian buffers of the White River watershed, IN (USA)

Riparian ecosystems are defined by the nature and regularity of the interactions between a given river system and its floodplains, and past studies have often presented vegetation cover as the exclusive expression of these interactions. There has been to our knowledge, no systematic attempt at linking greenhouse gases (GHG) fluxes and types of riparian buffers. The present study was conducted to investigate the intensity and seasonality of carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) fluxes in riparian buffers in three common hydro-geomorphic settings (HGM) across the White River watershed (Indiana, USA). These classes included riparian sites located: (i) in till plain depressions near 1st order streams (HGM-1), (ii) in incised narrow valleys with thin alluvium layers above glacial till (HGM-2), and (iii) along 3rd–4th order streams in broad floodplains with thick alluvial and glacial outwash deposits (HGM-3). For each class, 3 sites were selected and GHG fluxes were measured during the wet (May) and dry seasons (August). Strong relationships were found between GHG fluxes, soil properties and environmental factors, but these relationships varied with season and gas species, making it challenging to rely on these relationships for GHG fluxes upscaling. Analysis of variance and discriminant analysis showed that the HGM-defined riparian buffers were distinct in terms of GHG flux intensity. Regardless of season, the HGM-1 sites emitted CO2 at rates 1.6 times higher than at the other sites, likely due to difference in soil C quality. During the wet season, N2O emission was significantly higher at the HGM-3 than at the other sites (0.88 vs 0.27 mg N m− 2 d− 1), and was negatively related with the gradient of the adjacent channel (r2: 0.69). The riparian buffers acted as CH4 sinks, with the HGM-2 sites exhibiting CH4 uptake rates significantly greater than the other riparian types (− 0.80 vs − 0.34 mg CH4-C m− 2 d− 1). The consistency of these results underscores the potential of an HGM-based monitoring approach to derive watershed-scale GHG budgets for riparian buffers

Dissolved carbon and CDOM in lake ice and underlying waters along a salinity gradient in shallow lakes of Northeast China

The variations of DOC and DIC concentrations in lake ice and underlying waters were examined in 40 shallow lakes across the Songnen Plain, Northeast China. The lakes, frozen annually during winter, included freshwater and brackish systems (EC > 1000 μS cm−1; range: 171–12607 μS cm−1 in underlying water). Results showed that lake ice contained lower DOC (7.2 mg L−1) and DIC (6.7 mg L−1) concentration compared to the underlying waters (58.2 and 142.4 mg L−1, respectively). Large differences in DOC and DIC concentrations of underlying waters were also observed between freshwater (mean ± SD: 22.3 ± 11.5 mg L−1, 50.7 ± 20.6 mg L−1) and brackish lakes (83.3 ± 138.0 mg L−1, 247.0 ± 410.5 mg L−1). A mass balance model was developed to describe the relative distribution of solutes and chemical attributes between ice and the underlying waters. Results showed that water depth and ice thickness were the key factors regulating the spatial distribution of solutes in the frozen lakes. Chromophoric dissolved organic matter (CDOM) absorption coefficient at 320 nm, aCDOM(320) and specific UV absorbance (SUVA254) were used to characterize CDOM composition and quality. Compared to the underlying waters, CDOM present in ice largely included low aromaticity organic substances, an outcome perhaps facilitated by ice formation and photo-degradation. In ice and underlying freshwaters, CDOM predominantly included organic C fractions of high aromaticity, while low aromaticity organic substances were observed for brackish lakes. Results of this study suggest that, if water salinity increases due to climate change and anthropogenic activities, significant changes can occur in the dissolved carbon and fate of CDOM in these shallow lakes

Drought effects on root and tuber production: A meta-analysis

Roots and tubers such as potatoes and cassava rank within the top six among the world’s most important food crops, yet the extent to which their global production has been adversely affected by drought remains unclear. Greater uncertainties exist on how drought effects co-vary with: (1) root and tuber species, (2) soil texture, (3) agro-ecological region, and 4) drought timing. It is often assumed that potato is drought-sensitive whereas cassava and sweet potato are resistant to drought, but this assumption has not been quantitatively tested. To address these uncertainties, we collected literature data between 1980 and 2015 that reported monoculture root and tuber yield responses to drought under field conditions, and analyzed this large data set using meta-analysis technique. Our results showed that the amount of water reduction was positively related with yield reduction, but the extent of the impact varied with root or tuber species and the phenological phase during which drought occurred. In contrast to common assumptions regarding drought resistance of certain root and tuber crops, we found that yield reduction was similar between potato and species thought to be drought-resistant such as cassava and sweet potato. Here we suggest that drought-resistance in cassava and sweet potato could be more related to survival rather than yield. All root or tuber crops, however, experienced greater yield reduction when drought struck during the tuberization period compared to during their vegetative phase. The effect of soil texture on yield reduction was less obvious, and similarly we did not find any significant effects of region (and related climatic factors) on either yield reduction or drought sensitivity. Our study provides useful information that can inform agricultural planning, and influence the direction of research for improving the productivity and resilience of these under-utilized crops in the drought-prone regions of the world

Global synthesis of drought effects on food legume production

poster abstractFood legume crops play important roles in conservation farming systems, contribute to human nutrition and food security, yet in many regions of the world, their production has been adversely affected by drought. Currently, it remains unclear how the effects drought co-vary with legume species, soil texture, agroclimatic region, drought timing and intensity. To address these uncertainties, we collected literature data (1705 data points, averaged into 676 data points) between 1980 and 2014 that reported monoculture legume yield responses to drought under field conditions and analyzed this extensive data set using metaanalysis techniques. We performed unweighted analysis using the log response ratio (lnR) to calculate the bootstrapped confidence limits of those responses for each potential factor. Our results indicated that the amount of water reduction was positively related with yield reduction, but the extent of the impact varied with legume species and the phenological state during which drought occurred. Overall, field pea (Pisum sativum), groundnut (Arachis hypogea), and pigeon pea (Cajanus cajan) were found to experience lower yield reduction due to drought compared to legumes such as lablab beans (Dolichos lablab) or black grams (Vigna mungo). Although yield reduction was generally greater when legumes experienced drought during their reproductive stage compared to during their vegetative stage, legumes were sensitive to drought at all growth stages. Legumes planted in medium-textured soils also exhibited greater yield reduction compared to those planted in coarse- or fine-textured soils. In contrast, regions and their associated climatic factors were less associated with legume yield reduction. The study provides useful insights for legume agricultural planning and the direction of potential development of drought-resistant legume species to improve food security in the drought-prone regions of the world

Global synthesis of drought effects on cereal production

poster abstractAbstract Drought has been a major cause of agricultural disaster, yet how various factors (e.g., crop species, phenological phases) affect the vulnerability of cereal agriculture to drought remains unclear. Using a data synthesis approach, this study aims to better characterize the effects of these factors and to provide critical information on minimizing yield loss. We collected data from peer-reviewed publications between 1980 and 2015 which examined cereal yield responses to drought using field experiments. We performed unweighted analysis using the log response ratio to calculate the bootstrapped confidence limits of yield responses and calculated drought sensitivities for several key factors. Our results showed that yield reduction varied with species, with wheat having lower sensitivity to drought and yield reduction (20.6%) compared to maize (39.3%) at approximately 60% water reduction. Drought that occurred during the reproductive phase caused greater yield reduction (30%) than when it occurred during the vegetative phase (20%). While cereal cultivation in the drylands was more prone to yield loss than in the non-dryland regions, no difference was observed among sites of different soil texture. Informed by these results, we discuss possible causes and low-cost strategies that may minimize drought effect on crop yield

Global synthesis of drought effects on cereal, legume, tuber and root crops production: A review

As a result of climate change, drought is predicted to pose greater pressure on food production system than in the past. At the same time, crop yield co-varies with both environmental (e.g., water, temperature, aridity) and agronomic variables (i.e., crop species, soil texture, phenological phase). To improve our quantitative understanding on the effects of these co-varying factors on agricultural productivity, we synthesized previous meta-analysis studies summarizing the results of numerous independent field experiments on drought and its effect on the production of cereal, legume, root and/or tuber (root/tuber) crops. We also included new crops species that were not covered in previous meta-analyses and the effects of heat stress. Our results indicated that cereals tended to be more drought resistant than legumes and root/tubers. Most crops were more sensitive to drought during their reproductive (i.e., grains filling, tuber initiation) than during their vegetative phase, except for wheat, which was also sensitive during vegetative phase. Recovery from drought impact at reproductive phase was either: (i) unfeasible for crops experiencing damage to their reproductive organs (e.g., maize, rice) or (ii) limited for root/tuber crops, provided that water was abundant during the subsequent root/tuber bulking period. Across soil texture, the variability of yield reduction for cereals was also lower in comparison to legume or root/tuber crops, probably due to the extensive and deep rooting system of cereal crops. As crop species, plant phenology, and soil texture were important co-varying factors in determining drought-induced crop yield reduction, no single approach would be sufficient to improve crop performance during drought. Consequently, a combination of approaches, particularly site-specific management practices that consider soil conditions (i.e., intercropping, mulching, and crop rotation) and selection of crop varieties adjusted to the local climate should be adopted in order to improve the sustainability of agricultural production in a changing climate

Meta-Analysis of Phosphorus Loss from No-Till Soils

Agriculture is a significant contributor to phosphorus (P) enrichment in aquatic ecosystems. No-till (NT) farming has been proposed as an alternative approach to conventional tillage (CT) in reducing soil P export, but published data have shown contrasting impacts, likely due to the interacting effects of different physical (climate region, rainfall variability, transport pathway, slope gradient) and management variables (NT duration, crop species). We conducted a meta-analysis to understand the extent to which each of these variables controls the concentration and load of different P fractions (dissolved P, particulate P) in agricultural runoff and leaching. In comparison with CT, particulate P loss was significantly lower with NT adoption (45 and 55% reduction in concentration and load, respectively), but an increase in dissolved P loss was observed. The extent of the reduction or increase, however, varied with different physical and management variables. In comparison with CT, for example, NT was not effective in reducing particulate P concentration during wet years and particulate P load on steep slopes (4–9%). Total P concentration was also similar with CT at sites under prolonged NT duration (∼10 yr) and at NT fields planted with soybean [Glycine max (L.) Merr.]. Our results underscore the need to consider the covarying physical and management factors when assessing the potential of NT farming in controlling P loss in the environment. The limited impact of NT on dissolved P loss remains a serious impediment toward harnessing the water quality benefits of this management practice

Can ridge-furrow plastic mulching replace irrigation in dryland wheat and maize cropping systems?

Dryland crop production requires significant water investments, but problems associated with irrigation have been observed in many dryland regions (e.g., China, Australia and the Mediterranean basin). A key strategy for maintaining crop yields without over-exploiting the scarce water resource is by increasing water use efficiency (WUE). Plastic mulching technology for wheat and maize has been commonly used in China, but their effect on yield, soil water content, evapotranspiration (ET), and WUE has not been compared with traditional irrigation. Using a meta-analysis approach, we quantitatively examined the efficacy of plastic mulching in comparison with traditional irrigation in the same region. By covering the ridges with plastic and channeling rainwater into a very narrow planting zone (furrow), our results showed that plastic mulching resulted in a yield increase comparable to irrigated crops but used 24% less water in comparison with irrigation due primarily to a much greater WUE and better retention of soil water. The higher WUE in plastic-mulched croplands was likely a result of a greater proportion of available water being used for transpiration (T) than evaporation (E). Currently production costs and residual plastic pollution hinder worldwide adoption of the technique, despite being a promising strategy for dryland cropping systems

Impacts of no-tillage management on nitrate loss from corn, soybean and wheat cultivation: A meta-analysis

Although no-till (NT) has been promoted as an alternative land management practice to conventional tillage (CT), its impact on water quality, especially nitrate (NO3 −) loss remain controversial. We conducted a meta-analysis to compare NO3 − concentration and load in NT and CT systems via two major transport pathways: runoff and leaching. Rainfall variability, aridity, soil texture, tillage duration, crop species, and fertilizer type were used as co-varying factors. In comparison to CT, NT resulted in an overall increase of runoff NO3 − concentration, but similar runoff NO3 − load. In contrast, leachate NO3 − load was greater under NT than under CT, although leachate NO3 − concentration was similar under both tillage practices, indicating that the effect of NT on NO3 − load was largely determined by changes in water flux. Some deviations from these overall trends, however, were recorded with different co-varying variables. In comparison to CT, NT, for example, generated lower leachate NO3 − concentration and similar (instead of elevated) NO3 − leachate load from soybean fields (no N fertilizer applied). These results suggest NT needs to be complemented with other practices (e.g., cover crops, reduced N rate, split N application) in order to improve soil N retention and water quality benefits