Taiwan's New Southbound Policy : accomplishments and perceptions

For more about the East-West Center, see http://www.eastwestcenter.org/Ja Ian Chong, Associate Professor, National University of Singapore, explains that "NSP success requires... fostering greater public understanding and support domestically.

Reduced TCA cycle rates at high hydrostatic pressure hinder hydrocarbon degradation and obligate oil degraders in natural, deep-sea microbial communities

Petroleum hydrocarbons reach the deep-sea following natural and anthropogenic factors. The process by which they enter deep-sea microbial food webs and impact the biogeochemical cycling of carbon and other elements is unclear. Hydrostatic pressure (HP) is a distinctive parameter of the deep sea, although rarely investigated. Whether HP alone affects the assembly and activity of oil-degrading communities remains to be resolved. Here we have demonstrated that hydrocarbon degradation in deep-sea microbial communities is lower at native HP (10 MPa, about 1000 m below sea surface level) than at ambient pressure. In long-term enrichments, increased HP selectively inhibited obligate hydrocarbon-degraders and downregulated the expression of beta-oxidation-related proteins (i.e., the main hydrocarbon-degradation pathway) resulting in low cell growth and CO2 production. Short-term experiments with HP-adapted synthetic communities confirmed this data, revealing a HP-dependent accumulation of citrate and dihydroxyacetone. Citrate accumulation suggests rates of aerobic oxidation of fatty acids in the TCA cycle were reduced. Dihydroxyacetone is connected to citrate through glycerol metabolism and glycolysis, both upregulated with increased HP. High degradation rates by obligate hydrocarbon-degraders may thus be unfavourable at increased HP, explaining their selective suppression. Through lab-scale cultivation, the present study is the first to highlight a link between impaired cell metabolism and microbial community assembly in hydrocarbon degradation at high HP. Overall, this data indicate that hydrocarbons fate differs substantially in surface waters as compared to deep-sea environments, with in situ low temperature and limited nutrients availability expected to further prolong hydrocarbons persistence at deep sea

The Effect of Iron Limitation on the Transcriptome and Proteome of Pseudomonas fluorescens Pf-5

One of the most important micronutrients for bacterial growth is iron, whose bioavailability in soil is limited. Consequently, rhizospheric bacteria such as Pseudomonas fluorescens employ a range of mechanisms to acquire or compete for iron. We investigated the transcriptomic and proteomic effects of iron limitation on P. fluorescens Pf-5 by employing microarray and iTRAQ techniques, respectively. Analysis of this data revealed that genes encoding functions related to iron homeostasis, including pyoverdine and enantio-pyochelin biosynthesis, a number of TonB-dependent receptor systems, as well as some inner-membrane transporters, were significantly up-regulated in response to iron limitation. Transcription of a ribosomal protein L36-encoding gene was also highly up-regulated during iron limitation. Certain genes or proteins involved in biosynthesis of secondary metabolites such as 2,4-diacetylphloroglucinol (DAPG), orfamide A and pyrrolnitrin, as well as a chitinase, were over-expressed under iron-limited conditions. In contrast, we observed that expression of genes involved in hydrogen cyanide production and flagellar biosynthesis were down-regulated in an iron-depleted culture medium. Phenotypic tests revealed that Pf-5 had reduced swarming motility on semi-solid agar in response to iron limitation. Comparison of the transcriptomic data with the proteomic data suggested that iron acquisition is regulated at both the transcriptional and post-transcriptional levels

Herding Cats: Coordination Challenges in ASEAN’s Approach to China

China Review-An Interdisciplinary Journal on Greater China231307-339Hong Kon

The collective influence of smaller states in the US-China security dilemma

For more about the East-West Center, see http://www.eastwestcenter.org/Ja Ian Chong, Asia Studies Visiting Fellow at the East-West Center in Washington, explains that "Any ratcheting down of Sino-US security dilemmas in the Asia-Pacific is a task that ultimately falls to Washington and Beijing, but regional states should avoid fueling tensions.

Revisiting responses to power preponderance : going beyond the balancing-bandwagoning dichotomy

Since the 1990s, there has been a growing body of literature in international relations that looks at the unipolar world order that emerged from the ashes of the Cold War. Most of these works, however, tend to focus on describing the characteristics of this unipolar world or predicting its longevity. This working paper contends that such approaches do not pay adequate attention to how non-leading states in the international system are attempting to respond to American primacy of power in this age of unipolarity. The author argues that conventional conceptions of international politics that frame state reactions to superior power within the bounds of balancing and bandwagoning are inadequate to understand how state actors are trying to advance and preserve interests in relation to preponderant American power

China's influence in Southeast Asia: No easy answers

China's Influence and the Center-periphery Tug of War in Hong Kong, Taiwan and Indo-Pacific257-27

Seeking Strategic Options for Myanmar: Reviewing Five-Point Consensus and Anticipating the Future of Democracy in Myanmar A Policy Paper Compendium

Seeking Strategic Options for Myanmar: Reviewing Five-Point Consensus and Anticipating the Future of Democracy in Myanmar A Policy Paper Compendiu